NPHS1 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NPHS1
Gene Name:  nephrosis 1, congenital, Finnish type (nephrin)
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100061574)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00350640
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3723nts
Open reading frame: 1 to 3723

ATGGCCCTGGGGACGACGCTCAGGGCTTCTCTCCTGCTCCTGGGGCTGCTGACTGAAGGC
CTGGCGCAGTTGGCGATTCCTGCCTCCGTTCCCCGGGGCTTCTGGGCCCTGCCTGAAAAC
CTGACGGTGGTGGAGGGGGCCTCAGTGGAGCTGCGTTGTGGGGTCAGCACCCCTGGCAGT
GCGGTGCAATGGGCCAAAGATGGGCTGCTCCTGGGCCCCGACCCCAGGATCCCAGGCTTC
CCGAGGTACCGCCTGGAAGGGGACCCTGCTAGAGGTGAATTCCACCTGCACATCGAGGCC
TGTGACCTCAGCGATGACGCGGAGTATGAGTGCCAGGTCGGCCGCTCTGAGATGGGGCCC
GAGCTCGTGTCTCCCAGAGTGATCCTCTCCATCCTGGTTCCTCCCAAGCTGCTCCTGCTG
ACCCCAGAGGCAGGCACCATGGTCACCTGGGTAGCTGGGCAGGAGTACGTGGTCAACTGT
GTGTCTGGGGACGCGAAGCCAGCACCTGACATCACCATTCTCCTGAGTGGACAGACAATA
TCTGACATCTCTGCAAACGTGAACGAGGGCTCCCAGCAGAAACTCTTCACTGTGGAGGCC
ACAGCCAGGGTGACACCCCGGAGCTCAGATAATAGGCAGTTGCTGGTCTGTGAGGCGTCT
AGCCCAGCACTGGAGGCCCCCATCAAGGCCTCATTCACCGTGAATGTTCTGTTCCCTCCA
GGACCCCCTGTCATCGAGTGGCCAGGCCTGGATGAGGGGCACGTGCGGGCAGGACAGAGC
TTGGAGCTGCCGTGCGTGGCCCGAGGGGGTAATCCCTTAGCCACACTGCAGTGGCTGAAG
AATGGCCAGCCGGTGTCCACAGCGTGGGGCACAGAGCACACCCAGGCGGTGGCCCGCAGT
GTGCTGGTGATGACCGTGAGGCCAGAAGACCATGGAGCGCAGCTCAGCTGCGAGGCCCAC
AACAGCGTGTCTGCAGGGACCCAGGAGCACGGCATCACACTGCAGGTCACCTTTCCCCCT
AGTGCCATTATTATCTTGGGATCTGCATCCCAGACTGAGAACAAGAACGTGACACTCTCC
TGTGTCAGCAAGTCCAGTCGCCCGCGGGTTCTGCTACGATGGTGGCTGGGCTGGCGGCAG
CTGCTGCCCATGGAGGAGACAGTCATGGATGGACTGCATGGCGGTCACATCTCCATGTCC
AACCTGACATTCCTGGCGCGGCGGGAGGACAACGGTCTGACCCTCACATGTGAGGCCTTC
AGTGAAGCCTTCACCAAGGAGACCTTCAAGAAGTCGCTCATCCTGAACGTAAAATATCCC
GCCCAGAAACTGTGGATTGAGGGTCCCCCAGAGGGCCAGAAGCTCCGGGCTGGGACCCGG
GTGAGGCTGGTGTGTTTGGCTATCGGGGGCAACCCAGAGCCCTCCCTCATGTGGTACAAG
GACTCGCGCACCGTGACCGAGTCGCGGCTGCCGCAGGAGTCGCGGCGCGTGCATCTCGGC
AGCGTGGAGAAATCTGGGAGCACCTTCTCCCGAGAGCTGGTGCTGGTCACAGGGCCGTCG
GACAACCAGGCCAAGTTCACGTGCAAGGCTGGACAGCTCAGCGCGTCCACGCAGCTGGCG
GTGCAGTTTCCCCCAACTAACGTGACGATCCTGGCCAACGCATCCGCACTGCGCCCGGGA
GACGCCTTAAACTTGACATGCGTCAGCGTCAGCAGCAATCCGCCGGTCAACTTGTCCTGG
GACAAGGAAGGGGAGAGGCTGGAGGGCGTGGCCGCCCCACCCCGGAGAGCCCCATTCAAA
GGCTCCGCCGCCGCCAGGAGCGTCCTTCTGCAAGTGTCATCCCGCGATCATGGCCAGCGC
GTGACCTGCCGCGCCCACAGCGCCGAGCTCCGCGAAACCGTGAGCTCCTTCTATCGCCTC
AACGTACTGTACCGTCCAGAGTTCCTGGGGGAGCAGGTGCTGGTGGTGACCGCGGTGGAG
CAGGGCGAGGCGTTGCTGCCCGTGTCCGTGTCCGCTAACCCCGCCCCCGAGGCCTTCAAC
TGGACCTTCCGCGGCTATCGCCTCAGTCCAGCGGGCGGCCCCCGGCATCGCATCCTGTCC
AGCGGGGCTCTGCATCTGTGGAATGTGACCCGCGCGGACGACGGCCTCTATCAGCTGCAC
TGCCAGAACTCTGAGGGCACCGCGGAAGCGCGGCTGCGGCTGGACGTGCACTATGCTCCC
ACCATCCGTGCCCTCCAGGACCCCACTGAGGTGAACGTCGGGGGTTCTGTGGACATAGTC
TGCACTGTCGATGCCAATCCCATCCTCCCGGGCATGTTCAACTGGGAGAGACTGGGAGAA
GATGAGGAGGACCAGAGCCTGGATGACATGGAGAAGATATCCAGGGGACCAACGGGGCGC
CTGCGGATTCACCATGCCAAACTGGCCCAGGCTGGCGCTTACCAGTGCATTGTGGACAAT
GGGGTGGCGCCTCCAGCACGACGGCTGCTCCGTCTTGTTGTCAGATTTGCCCCCCAGGTG
GAGCACCCCACTCCCCTAACTAAGGTGGCTGCAGCTGGAGACAGCACCAGTTCTGCCACC
CTCCACTGCCGTGCCCGAGGTGTCCCCAACATCGTTTTCACTTGGACAAAAAACGGGGTC
CCTCTGGATCTCCAAGATCCCAGGTACACGGAGCACACATACCACCAGGGTGGTGTCCAC
AGCAGCCTCCTGACCATTGCCAACGTGTCTGCCGCCCAGGATTACGCCCTCTTCACATGT
ACAGCCACCAACGCCCTTGGCTCGGACCAAACCAACATTCAACTTGTCAGCATCAGCCGC
CCTGACCCTCCATCAGGATTAAAGGTTGTGAGTCTGACCCCACACTCCGTGGGGCTGGAG
TGGAAGCCTGGCTTTGATGGGGGCCTGCCACAGAGGTTCTGCATCAGGTATGAGGCCCTG
GGGACTCCAGGGTTCCACTATGTGGATGTCGTACCACCCCAGGCCACCACCTTCACGCTG
ACTGGTCTACAGCCTTCTACAAGATACAGGGTCTGGCTGCTGGCCAGTAATGCCTTGGGG
GACAGTGGACTGGCTGACAAAGGGACCCAGCTTCCCATCACTACCCCAGGTCTCCACCAG
CCTTCTGGAGAACCTGAAGACCAGCTGCCCACAGAGCCACCTTCAGGACCCTCGGGGCTG
CCCCTGCTGCCTGTGCTGTTCGCTCTTGGGGGGCTTCTGCTCCTCTCCAATGCCTCCTGT
GTCGGGGGGGTCCTCTGGCAGCGGAGACTCAGGCGTCTTGCTGAGGGCATCTCAGAGAAG
ACAGAGGCAGGGTCGGAAGAGGACCGAGTCAGGAACGAATATGAGGAGAGCCAGTGGACA
GGAGAGCGGGACACTCAGAGCTCCACGGTCAGCACAACAGAGGCAGAGCCGTATTACCGC
TCCCTGAGGGACTTCAGCCCCCAGCTGCCCCCGACGCAGGAGGAGGTGTCTTATTCCCGA
GGTTTCACAGGTGAAGATGAGGATATGGCCTTCCCTGGGCACTTGTATGATGAGGTAGAA
AGAACGTACCCCCCGTCTGGAGCCTGGGGACCCCTCTACGATGAAGTGCAGATGGGACCC
TGGGACCTCCACTGGCCTGAAGACACATATCAGGATCCAAGAGGAATCTATGACCAGGTG
GCCGGAGACTTGGACACTCTGGAACCCGATTCTCTGCCCTTCGAGCTGAGGGGACATCTG
GTG

Protein sequence predicted based on nucleotide sequence

MALGTTLRASLLLLGLLTEGLAQLAIPASVPRGFWALPENLTVVEGASVELRCGVSTPGS
AVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGP
ELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTI
SDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPP
GPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARS
VLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLS
CVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAF
SEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYK
DSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLA
VQFPPTNVTILANASALRPGDALNLTCVSVSSNPPVNLSWDKEGERLEGVAAPPRRAPFK
GSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFYRLNVLYRPEFLGEQVLVVTAVE
QGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLH
CQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLGE
DEEDQSLDDMEKISRGPTGRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQV
EHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVH
SSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLE
WKPGFDGGLPQRFCIRYEALGTPGFHYVDVVPPQATTFTLTGLQPSTRYRVWLLASNALG
DSGLADKGTQLPITTPGLHQPSGEPEDQLPTEPPSGPSGLPLLPVLFALGGLLLLSNASC
VGGVLWQRRLRRLAEGISEKTEAGSEEDRVRNEYEESQWTGERDTQSSTVSTTEAEPYYR
SLRDFSPQLPPTQEEVSYSRGFTGEDEDMAFPGHLYDEVERTYPPSGAWGPLYDEVQMGP
WDLHWPEDTYQDPRGIYDQVAGDLDTLEPDSLPFELRGHLV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable