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CAPN12 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN12
Gene Name:  calpain 12
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100016452)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00350793

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2160nts         Open reading frame : 1 to 2160
Reference Sequence Alignment

ATGGCATCCAGCAGTGGGAGGGTCACCATCCAGCTCGTGGATGAGGAGGCTGGGGTCGGA
GCCGGGCGCCTGCAGCTTTTTCGGGGCCAGAGCTATGAGGCAATTCGGGCAGCCTGCCTG
GATTCGGGGATCCTGTTCCGCGACCCTTACTTCCCTGCTGGCCCTGATGCCCTTGGCTAT
GACCAGCTGGGGCCGGACTCGGAGAAGGCCAAAGGCGTGAAATGGATGAGGCCCCATGAG
TTCTGTGCTGAGCCGAAGTTCATCTGTGAAGACATGAGCCGCACAGACGTGTGTCAGGGG
AGCCTGGGTAACTGCTGGTTCCTTGCAGCCGCCGCCTCCCTTACTCTGTATCCCCGGCTC
CTGCGCCGGGTGGTCCCTCCTGGACAGGATTTCCAGCATGGCTACGCAGGCGTCTTCCAC
TTCCAGCTCTGGCAGTTTGGCCGCTGGATGGACGTCGTGGTGGATGACAGGCTGCCCGTG
CGTGAGGGGAAGCTGATGTTCGTGCGCTCGGAACAGCGGAATGAGTTCTGGGCCCCACTC
CTGGAGAAGGCCTACGCCAAGCTCCACGGCTCCTATGAGGTGATGCGGGGCGGCCACATG
AATGAGGCTTTTGTGGATTTCACAGGCGGCGTGGGCGAGGTGCTCTATCTGAGACAAAAC
AGCATGGGGCTGTTCTCTGCCCTGCGCCATGCCCTGGCCAAGGAGTCCCTCGTGGGCGCC
ACTGCCCTGAGTGATCGGGGTGAGTACCGCACAGAAGAGGGCCTGGTAAAGGGACACGCG
TATTCCATCACGGGCACACACAAGGTGTTCCTGGGCTTCACCAAGGTGCGGCTGCTGCGG
CTGCGGAACCCATGGGGCTGCGTGGAGTGGACGGGGGCCTGGAGCGACAGCTGCCCACGC
TGGGACACACTCCCCACCGAGTGCCGCGATGCCCTGCTGGTGAAAAAGGAGGATGGCGAG
TTCTGGATGGAGCTGCGGGACTTCCTCCTCCATTTCGACACCGTGCAGATCTGCTCGCTG
AGCCCGGAGGTGCTGGGCCCCAGCCCGGAGGGGGGCGGCTGGCACGTCCACACCTTCCAA
GGCCGCTGGGTGCGTGGCTTCAACTCCGGCGGGAGCCAGCCTAATGCTGAAACCTTCTGG
ACCAATCCTCAGTTCCGTTTAACGCTGCTGGAGCCTGATGAGGAGGATGACGAGGATGAG
GAAGGGCCCTGGGGGGGCTGGGGGGCTGCAGGGGCACGGGGCCCAGCGCGGGGGGGCCGC
ACGCCCAAGTGCACGGTCCTTCTGTCCCTCATCCAGCGCAACCGGCGGCGCCTGAGAGCC
AAGGGCCTCACTTACCTCACCGTTGGCTTCCACGTGTTCCAGATTCCAGAGGAGCTGCTG
GGCCTCTGGGATTCCCCGCGCAGCCATGCGCTCCTGCCCCGGCTGCTGCGCGCCGACCGC
TCGCCCCTCAGCGCCCGCCGCGACGTGACCCGCCGCTGCTGCCTGCGTCCAGGCCACTAC
CTGGTGGTGCCGAGCACCGCCCACGCCGGCGACGAGGCTGACTTCACTCTGCGTGTCTTC
TCCGAGCGCCGCCACACGGCCGTGGAGATCGACGACGTGATCAGCGCAGACCTGCAGTCT
CTCCAGGGCCCCTACCTGCCCCTGGAGCTGGGGTTGGAGCAGCTGTTTCAGGAGCTGGCT
GGAGAGGAGGAAGAACTCAATGCCTCTCAGCTCCAGGCCTTACTAAGCATTGCCCTGGAG
CCTGCCAGGGCCCATACCTCCACCCCCAGAGAGATCGGGCTCAGGACCTGTGAGCAGCTG
CTGCAGTGTTTCGGGCATGGGCAAAGCCTGGCCTTACACCACTTCCAGCAGCTCTGGGGC
TACCTCCTGGAGTGGCAGGCCATATTTAACAAGTTCGATGAGGACACCTCTGGAACCATG
AACTCCTACGAGCTGAGGCTGGCACTGAATGCAGCAGGCTTCCACCTGAACAACCAGCTG
ACCCAGACCCTCACCAGCCGCTACCGGGATAGCCGTCTGCGTGTGGACTTCGAGCGGTTC
GTGTCCTGTGTGGCCCACCTCACCTGCATCTTCTGCCACTGCAGCCAGCACCTGGATGGG
GGTGAGGGGGTCATCTGCCTGACCCACAGACAGTGGATGGAGGTGGCCACCTTCTCCTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN12
Symbol Nomenclature : CAPN12
Designation : CANP 12|calcium-activated neutral proteinase 12|calpain-12
Full Nomenclature : calpain 12
GENEID : 147968
GenBank Accession : 563400
HGNC : 13249
MIM : 608839
Vega : OTTHUMG00000182525
Target GenBank: BC153093

 

Reference Sequence Alignment

  

HsCD00350793      ATGGCATCCAGCAGTGGGAGGGTCACCATCCAGCTCGTGGATGAGGAGGCTGGGGTCGGA
NM_144691.3       ATGGCATCCAGCAGTGGGAGGGTCACCATCCAGCTCGTGGATGAGGAGGCTGGGGTCGGA
                  ************************************************************

HsCD00350793      GCCGGGCGCCTGCAGCTTTTTCGGGGCCAGAGCTATGAGGCAATTCGGGCAGCCTGCCTG
NM_144691.3       GCCGGGCGCCTGCAGCTTTTTCGGGGCCAGAGCTATGAGGCAATTCGGGCAGCCTGCCTG
                  ************************************************************

HsCD00350793      GATTCGGGGATCCTGTTCCGCGACCCTTACTTCCCTGCTGGCCCTGATGCCCTTGGCTAT
NM_144691.3       GATTCGGGGATCCTGTTCCGCGACCCTTACTTCCCTGCTGGCCCTGATGCCCTTGGCTAT
                  ************************************************************

HsCD00350793      GACCAGCTGGGGCCGGACTCGGAGAAGGCCAAAGGCGTGAAATGGATGAGGCCCCATGAG
NM_144691.3       GACCAGCTGGGGCCGGACTCGGAGAAGGCCAAAGGCGTGAAATGGATGAGGCCCCATGAG
                  ************************************************************

HsCD00350793      TTCTGTGCTGAGCCGAAGTTCATCTGTGAAGACATGAGCCGCACAGACGTGTGTCAGGGG
NM_144691.3       TTCTGTGCTGAGCCGAAGTTCATCTGTGAAGACATGAGCCGCACAGACGTGTGTCAGGGG
                  ************************************************************

HsCD00350793      AGCCTGGGTAACTGCTGGTTCCTTGCAGCCGCCGCCTCCCTTACTCTGTATCCCCGGCTC
NM_144691.3       AGCCTGGGTAACTGCTGGTTCCTTGCAGCCGCCGCCTCCCTTACTCTGTATCCCCGGCTC
                  ************************************************************

HsCD00350793      CTGCGCCGGGTGGTCCCTCCTGGACAGGATTTCCAGCATGGCTACGCAGGCGTCTTCCAC
NM_144691.3       CTGCGCCGGGTGGTCCCTCCTGGACAGGATTTCCAGCATGGCTACGCAGGCGTCTTCCAC
                  ************************************************************

HsCD00350793      TTCCAGCTCTGGCAGTTTGGCCGCTGGATGGACGTCGTGGTGGATGACAGGCTGCCCGTG
NM_144691.3       TTCCAGCTCTGGCAGTTTGGCCGCTGGATGGACGTCGTGGTGGATGACAGGCTGCCCGTG
                  ************************************************************

HsCD00350793      CGTGAGGGGAAGCTGATGTTCGTGCGCTCGGAACAGCGGAATGAGTTCTGGGCCCCACTC
NM_144691.3       CGTGAGGGGAAGCTGATGTTCGTGCGCTCGGAACAGCGGAATGAGTTCTGGGCCCCACTC
                  ************************************************************

HsCD00350793      CTGGAGAAGGCCTACGCCAAGCTCCACGGCTCCTATGAGGTGATGCGGGGCGGCCACATG
NM_144691.3       CTGGAGAAGGCCTACGCCAAGCTCCACGGCTCCTATGAGGTGATGCGGGGCGGCCACATG
                  ************************************************************

HsCD00350793      AATGAGGCTTTTGTGGATTTCACAGGCGGCGTGGGCGAGGTGCTCTATCTGAGACAAAAC
NM_144691.3       AATGAGGCTTTTGTGGATTTCACAGGCGGCGTGGGCGAGGTGCTCTATCTGAGACAAAAC
                  ************************************************************

HsCD00350793      AGCATGGGGCTGTTCTCTGCCCTGCGCCATGCCCTGGCCAAGGAGTCCCTCGTGGGCGCC
NM_144691.3       AGCATGGGGCTGTTCTCTGCCCTGCGCCATGCCCTGGCCAAGGAGTCCCTCGTGGGCGCC
                  ************************************************************

HsCD00350793      ACTGCCCTGAGTGATCGGGGTGAGTACCGCACAGAAGAGGGCCTGGTAAAGGGACACGCG
NM_144691.3       ACTGCCCTGAGTGATCGGGGTGAGTACCGCACAGAAGAGGGCCTGGTAAAGGGACACGCG
                  ************************************************************

HsCD00350793      TATTCCATCACGGGCACACACAAGGTGTTCCTGGGCTTCACCAAGGTGCGGCTGCTGCGG
NM_144691.3       TATTCCATCACGGGCACACACAAGGTGTTCCTGGGCTTCACCAAGGTGCGGCTGCTGCGG
                  ************************************************************

HsCD00350793      CTGCGGAACCCATGGGGCTGCGTGGAGTGGACGGGGGCCTGGAGCGACAGCTGCCCACGC
NM_144691.3       CTGCGGAACCCATGGGGCTGCGTGGAGTGGACGGGGGCCTGGAGCGACAGCTGCCCACGC
                  ************************************************************

HsCD00350793      TGGGACACACTCCCCACCGAGTGCCGCGATGCCCTGCTGGTGAAAAAGGAGGATGGCGAG
NM_144691.3       TGGGACACACTCCCCACCGAGTGCCGCGATGCCCTGCTGGTGAAAAAGGAGGATGGCGAG
                  ************************************************************

HsCD00350793      TTCTGGATGGAGCTGCGGGACTTCCTCCTCCATTTCGACACCGTGCAGATCTGCTCGCTG
NM_144691.3       TTCTGGATGGAGCTGCGGGACTTCCTCCTCCATTTCGACACCGTGCAGATCTGCTCGCTG
                  ************************************************************

HsCD00350793      AGCCCGGAGGTGCTGGGCCCCAGCCCGGAGGGGGGCGGCTGGCACGTCCACACCTTCCAA
NM_144691.3       AGCCCGGAGGTGCTGGGCCCCAGCCCGGAGGGGGGCGGCTGGCACGTCCACACCTTCCAA
                  ************************************************************

HsCD00350793      GGCCGCTGGGTGCGTGGCTTCAACTCCGGCGGGAGCCAGCCTAATGCTGAAACCTTCTGG
NM_144691.3       GGCCGCTGGGTGCGTGGCTTCAACTCCGGCGGGAGCCAGCCTAATGCTGAAACCTTCTGG
                  ************************************************************

HsCD00350793      ACCAATCCTCAGTTCCGTTTAACGCTGCTGGAGCCTGATGAGGAGGATGACGAGGATGAG
NM_144691.3       ACCAATCCTCAGTTCCGTTTAACGCTGCTGGAGCCTGATGAGGAGGATGACGAGGATGAG
                  ************************************************************

HsCD00350793      GAAGGGCCCTGGGGGGGCTGGGGGGCTGCAGGGGCACGGGGCCCAGCGCGGGGGGGCCGC
NM_144691.3       GAAGGGCCCTGGGGGGGCTGGGGGGCTGCAGGGGCACGGGGCCCAGCGCGGGGGGGCCGC
                  ************************************************************

HsCD00350793      ACGCCCAAGTGCACGGTCCTTCTGTCCCTCATCCAGCGCAACCGGCGGCGCCTGAGAGCC
NM_144691.3       ACGCCCAAGTGCACGGTCCTTCTGTCCCTCATCCAGCGCAACCGGCGGCGCCTGAGAGCC
                  ************************************************************

HsCD00350793      AAGGGCCTCACTTACCTCACCGTTGGCTTCCACGTGTTCCAGATTCCAGAGGAGCTGCTG
NM_144691.3       AAGGGCCTCACTTACCTCACCGTTGGCTTCCACGTGTTCCAGATTCCAGAGGAGCTGCTG
                  ************************************************************

HsCD00350793      GGCCTCTGGGATTCCCCGCGCAGCCATGCGCTCCTGCCCCGGCTGCTGCGCGCCGACCGC
NM_144691.3       GGCCTCTGGGATTCCCCGCGCAGCCATGCGCTCCTGCCCCGGCTGCTGCGCGCCGACCGC
                  ************************************************************

HsCD00350793      TCGCCCCTCAGCGCCCGCCGCGACGTGACCCGCCGCTGCTGCCTGCGTCCAGGCCACTAC
NM_144691.3       TCGCCCCTCAGCGCCCGCCGCGACGTGACCCGCCGCTGCTGCCTGCGTCCAGGCCACTAC
                  ************************************************************

HsCD00350793      CTGGTGGTGCCGAGCACCGCCCACGCCGGCGACGAGGCTGACTTCACTCTGCGTGTCTTC
NM_144691.3       CTGGTGGTGCCGAGCACCGCCCACGCCGGCGACGAGGCTGACTTCACTCTGCGTGTCTTC
                  ************************************************************

HsCD00350793      TCCGAGCGCCGCCACACGGCCGTGGAGATCGACGACGTGATCAGCGCAGACCTGCAGTCT
NM_144691.3       TCCGAGCGCCGCCACACGGCCGTGGAGATCGACGACGTGATCAGCGCAGACCTGCAGTCT
                  ************************************************************

HsCD00350793      CTCCAGGGCCCCTACCTGCCCCTGGAGCTGGGGTTGGAGCAGCTGTTTCAGGAGCTGGCT
NM_144691.3       CTCCAGGGCCCCTACCTGCCCCTGGAGCTGGGGTTGGAGCAGCTGTTTCAGGAGCTGGCT
                  ************************************************************

HsCD00350793      GGAGAGGAGGAAGAACTCAATGCCTCTCAGCTCCAGGCCTTACTAAGCATTGCCCTGGAG
NM_144691.3       GGAGAGGAGGAAGAACTCAATGCCTCTCAGCTCCAGGCCTTACTAAGCATTGCCCTGGAG
                  ************************************************************

HsCD00350793      CCTGCCAGGGCCCATACCTCCACCCCCAGAGAGATCGGGCTCAGGACCTGTGAGCAGCTG
NM_144691.3       CCTGCCAGGGCCCATACCTCCACCCCCAGAGAGATCGGGCTCAGGACCTGTGAGCAGCTG
                  ************************************************************

HsCD00350793      CTGCAGTGTTTCGGGCATGGGCAAAGCCTGGCCTTACACCACTTCCAGCAGCTCTGGGGC
NM_144691.3       CTGCAGTGTTTCGGGCATGGGCAAAGCCTGGCCTTACACCACTTCCAGCAGCTCTGGGGC
                  ************************************************************

HsCD00350793      TACCTCCTGGAGTGGCAGGCCATATTTAACAAGTTCGATGAGGACACCTCTGGAACCATG
NM_144691.3       TACCTCCTGGAGTGGCAGGCCATATTTAACAAGTTCGATGAGGACACCTCTGGAACCATG
                  ************************************************************

HsCD00350793      AACTCCTACGAGCTGAGGCTGGCACTGAATGCAGCAGGCTTCCACCTGAACAACCAGCTG
NM_144691.3       AACTCCTACGAGCTGAGGCTGGCACTGAATGCAGCAGGCTTCCACCTGAACAACCAGCTG
                  ************************************************************

HsCD00350793      ACCCAGACCCTCACCAGCCGCTACCGGGATAGCCGTCTGCGTGTGGACTTCGAGCGGTTC
NM_144691.3       ACCCAGACCCTCACCAGCCGCTACCGGGATAGCCGTCTGCGTGTGGACTTCGAGCGGTTC
                  ************************************************************

HsCD00350793      GTGTCCTGTGTGGCCCACCTCACCTGCATCTTCTGCCACTGCAGCCAGCACCTGGATGGG
NM_144691.3       GTGTCCTGTGTGGCCCACCTCACCTGCATCTTCTGCCACTGCAGCCAGCACCTGGATGGG
                  ************************************************************

HsCD00350793      GGTGAGGGGGTCATCTGCCTGACCCACAGACAGTGGATGGAGGTGGCCACCTTCTCCTAA
NM_144691.3       GGTGAGGGGGTCATCTGCCTGACCCACAGACAGTGGATGGAGGTGGCCACCTTCTCCTAG
                  ***********************************************************.

Pathways

Panther : Huntington disease

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
UniProt : Q6ZSI9
HPRD : 07007

Clone

Cloning Information : 563400

Organism


TAX_ID : 9606
Species Specific ID: 147968

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000182472

Reagents

Labome : CAPN12-antibody