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H1F0 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  H1F0
Gene Name:  H1 histone family, member 0
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100000263)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00350988

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 582nts         Open reading frame : 1 to 582

ATGACCGAGAATTCCACGTCCGCCCCTGCGGCCAAGCCCAAGCGGGCCAAGGCCTCCAAG
AAGTCCACAGACCACCCCAAGTATTCAGACATGATCGTGGCTGCCATCCAGGCCGAGAAG
AACCGCGCTGGCTCCTCGCGCCAGTCCATTCAGAAGTATATCAAGAGCCACTACAAGGTG
GGTGAGAACGCTGACTCGCAGATCAAGTTGTCCATCAAGCGCCTGGTCACCACCGGTGTC
CTCAAGCAGACCAAAGGGGTGGGGGCCTCGGGGTCCTTCCGGCTAGCCAAGAGCGACGAA
CCCAAGAAGTCAGTGGCCTTCAAGAAGACCAAGAAGGAAATCAAGAAGGTAGCCACGCCA
AAGAAGGCATCCAAGCCCAAGAAGGCTGCCTCCAAAGCCCCAACCAAGAAACCCAAAGCC
ACCCCGGTCAAGAAGGCCAAGAAGAAGCTGGCTGCCACGCCCAAGAAAGCCAAAAAACCC
AAGACTGTCAAAGCCAAGCCGGTCAAGGCATCCAAGCCCAAAAAGGCCAAACCAGTGAAA
CCCAAAGCAAAGTCCAGTGCCAAGAGGGCCGGCAAGAAGAAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : H1F0
Symbol Nomenclature : H1F0
SYNONYM : H10; H1FV
Designation : H1.0, H1(0), H1-0|histone H1'|histone H1(0)|histone H1.0
Full Nomenclature : H1 histone family, member 0
GENEID : 3005
GenBank Accession : 563595
HGNC : 4714
MIM : 142708
Vega : OTTHUMG00000150659
Target GenBank: CU013388

 

Pathways

Reactome : Activation of DNA fragmentation factor
Reactome : Apoptosis
Reactome : Apoptosis induced DNA fragmentation
Reactome : Apoptotic execution phase
Reactome : Cellular Senescence
Reactome : Cellular responses to stress
Reactome : DNA Damage/Telomere Stress Induced Senescence
Reactome : Formation of Senescence-Associated Heterochromatin Foci (SAHF)

Protein

SMART domain : H15 : Domain in histone families 1 and 5
UniProt : P07305
HPRD : 00819

Clone

Cloning Information : 563595

Organism


TAX_ID : 9606
Species Specific ID: 3005

Genome

Chromosome : 22
Map Location : 22q13.1
Ensembl : ENSG00000189060

Reagents

Labome : H1F0-antibody