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FABP6 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FABP6
Gene Name:  fatty acid binding protein 6, ileal
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100071757)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00351968

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 384nts         Open reading frame : 1 to 384
Reference Sequence Alignment

ATGGCTTTCACCGGCAAGTTCGAGATGGAGAGTGAGAAGAATTATGATGAGTTCATGAAG
CTCCTTGGGATCTCCAGCGATGTAATCGAAAAGGCCCACAACTTCAAGATCGTCACGGAG
GTGCAGCAGGATGGGCAGGACTTCACTTGGTCCCAGCACTACTACGGGGGCCACACCATG
ACCAACAAGTTCACTGTTGGCAAGGAAAGCAACATACAGACAATGGGGGGCAAGACGTTC
AAGGCCACTGTGCAGATGGAGGGCGGGAAGCTGGTGGTGAATTTCCCCAACTATCACCAG
ACCTCAGAGATCGTGGGTGACAAGCTGGTGGAGGTCTCCACCATCGGAGGCGTGACCTAT
GAGCGCGTGAGCAAGAGACTGGCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FABP6
Symbol Nomenclature : FABP6
SYNONYM : I-15P; I-BABP; I-BALB; I-BAP; ILBP; ILBP3; ILLBP
Designation : GT|gastrotropin|ileal bile acid binding protein|ileal lipid-binding protein|illeal lipid-binding protein|intestinal 15 kDa protein
Full Nomenclature : fatty acid binding protein 6, ileal
GENEID : 2172
GenBank Accession : 564575
HGNC : 3561
MIM : 600422
Vega : OTTHUMG00000130329
Target GenBank: HQ448339

 

Reference Sequence Alignment

  

HsCD00351968        ------------------------------------------------------------
NM_001040442.1      ATGAAGACAGTGACGATGATGATGGTGGTGGAGATGCAGGCGCTGACTCAGGTTCTGAGA
NM_001130958.1      ATGAAGACAGTGACGATGATGATGGTGGTGGAGATGCAGGCGCTGACTCAGGTTCTGAGA
NM_001445.2         ------------------------------------------------------------
                                                                                

HsCD00351968        ------------------------------------------------------------
NM_001040442.1      GCTGTGTTGTCTGCGTGCACATGGGTGAGCCGGAAAGGAGACCTGCAGAGAATGAAACAG
NM_001130958.1      GCTGTGTTGTCTGCGTGCACATGGGTGAGCCGGAAAGGAGACCTGCAGAGAATGAAACAG
NM_001445.2         ------------------------------------------------------------
                                                                                

HsCD00351968        ---------------------------ATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
NM_001040442.1      ACACATAAAGGAAAGCCTCCCAGCAGCATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
NM_001130958.1      ACACATAAAGGAAAGCCTCCCAGCAGCATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
NM_001445.2         ---------------------------ATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
                                               *********************************

HsCD00351968        GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
NM_001040442.1      GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
NM_001130958.1      GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
NM_001445.2         GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
                    ************************************************************

HsCD00351968        GCCCACAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
NM_001040442.1      GCCCGCAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
NM_001130958.1      GCCCGCAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
NM_001445.2         GCCCGCAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
                    ****.*******************************************************

HsCD00351968        CAGCACTACTACGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
NM_001040442.1      CAGCACTACTCCGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
NM_001130958.1      CAGCACTACTCCGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
NM_001445.2         CAGCACTACTCCGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
                    **********.*************************************************

HsCD00351968        ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
NM_001040442.1      ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
NM_001130958.1      ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
NM_001445.2         ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
                    ************************************************************

HsCD00351968        GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
NM_001040442.1      GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
NM_001130958.1      GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
NM_001445.2         GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
                    ************************************************************

HsCD00351968        GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCC---
NM_001040442.1      GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCCTAA
NM_001130958.1      GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCCTAA
NM_001445.2         GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCCTAA
                    ***************************************************   

Pathways

Reactome : Bile acid and bile salt metabolism
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Recycling of bile acids and salts

Protein

UniProt : P51161
HPRD : 02689

Clone

Cloning Information : 564575

Organism


TAX_ID : 9606
Species Specific ID: 2172

Genome

Chromosome : 5
Map Location : 5q33.3-q34
Ensembl : ENSG00000170231

Reagents

Labome : FABP6-antibody