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LGALS9C (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS9C
Gene Name:  lectin, galactoside-binding, soluble, 9C
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100071568)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00352083

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1068nts         Open reading frame : 1 to 1068
Reference Sequence Alignment

ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
CCTCCCATCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS9C
Symbol Nomenclature : LGALS9C
Designation : gal-9C|galectin 9 like|galectin-9-like protein B|galectin-9C|lectin, galactoside-binding, soluble, 9 (galectin 9) pseudogene
Full Nomenclature : lectin, galactoside-binding, soluble, 9C
GENEID : 654346
GenBank Accession : 564690
HGNC : 33874
Vega : OTTHUMG00000059251
Target GenBank: HQ448175

 

Reference Sequence Alignment

  

HsCD00352083        ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
NM_009587.2         ATGGCCTTCAGCGGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACT
NM_001040078.2      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
XM_005256769.1      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
XM_005256770.1      ATGGCCTTCAGCGGTTGCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
NM_001042685.1      ATGGCCTTCAGCGGTTCCCAGGCTCCCTATCTGAGCCCAGCCGTCCCCTTTTCTGGGACT
                    **************** ************ ***** ***** ******************

HsCD00352083        ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
NM_009587.2         ATTCAAGGAGGTCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCC
NM_001040078.2      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
XM_005256769.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
XM_005256770.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTGC
NM_001042685.1      ATCCAAGGGGGTCTCCAGGACGGATTTCAGATCACTGTCAATGGGGCCGTTCTCAGCTCC
                    ** *****.*************** ********************.************ *

HsCD00352083        AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
NM_009587.2         AGTGGAACCAGGTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTC
NM_001040078.2      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
XM_005256769.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
XM_005256770.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
NM_001042685.1      AGTGGAACCAGGTTTGCTGTGGACTTTCAGACGGGCTTCAGTGGAAACGACATTGCCTTC
                    *********************.********** ************** ************

HsCD00352083        CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
NM_009587.2         CACTTCAACCCTCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGA
NM_001040078.2      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
XM_005256769.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
XM_005256770.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
NM_001042685.1      CACTTCAACCCTCGGTTTGAAGACGGAGGGTATGTGGTGTGCAACACGAGGCAGAAAGGA
                    *********************** ******** ***********************.***

HsCD00352083        ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
NM_009587.2         AGCTGGGGGCCCGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGAC
NM_001040078.2      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
XM_005256769.1      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
XM_005256770.1      ACATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
NM_001042685.1      AGATGGGGGCCCGAGGAGAGGAAGATGCACATGCCCTTCCAGAAGGGGATGCCCTTTGAC
                    * .********************** .******** ************************

HsCD00352083        CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
NM_009587.2         CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTG
NM_001040078.2      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
XM_005256769.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
XM_005256770.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
NM_001042685.1      CTCTGCTTCCTGGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGAGCCTCTTCGTG
                    ************************************************* **********

HsCD00352083        CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_009587.2         CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_001040078.2      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
XM_005256769.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
XM_005256770.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
NM_001042685.1      CAGTACTTCCACCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTG
                    ************************************************************

HsCD00352083        CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
NM_009587.2         CAGCTGTCCTACATCAGCTTCCAGAACCCCCGCACAGTCCCTGTTCAGCCTGCCTTCTCC
NM_001040078.2      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
XM_005256769.1      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCGCAGTCCCCGTTCAGCCTGCCTTCTCC
XM_005256770.1      CAGCTGTCCTACATCAGCTTCCAGCCTCCCAGCGTG------------------------
NM_001042685.1      CAGCTGTCCTACATCAGCTTCCAGAATCCCCGCACAGTCCCCGTTCAGCCTGCCTTCTCC
                    ************************.. ***.**. .                        

HsCD00352083        ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_009587.2         ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
NM_001040078.2      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
XM_005256769.1      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
XM_005256770.1      ------------------------------------------------------------
NM_001042685.1      ACGGTGCCGTTCTCCCAGCCTGTCTGTTTCCCACCCAGGCCCAGGGGGCGCAGACAAAAA
                                                                                

HsCD00352083        CCTCCCATCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
NM_009587.2         CCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTG
NM_001040078.2      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
XM_005256769.1      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
XM_005256770.1      ------------CGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
NM_001042685.1      CCTCCCAGCGTGCGGCCTGCCAACCCAGCTCCCATTACCCAGACAGTCATCCACACGGTG
                                 *************.*****************************.***

HsCD00352083        CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
NM_009587.2         CAGAGCGCCCCTGGACAGATGTTCTCTACT---CCCGCCATCCCACCTATGATGTACCCC
NM_001040078.2      CAGAGTGCCTCTGGACAGATGTTCTCTCAGACTCCCGCCATCCCACCTATGATGTACCCC
XM_005256769.1      CAGAGTGCCTCTGGACAGATGTTCTCTAC---TCCCGCCATCCCACCTATGATGTACCCC
XM_005256770.1      CAGAGTGCCTCTGGACAGATGTTCTCTAC---TCCCGCCATCCCACCTATGATGTACCCC
NM_001042685.1      CAGAGCGCCTCTGGACAGATGTTCTCTACT---CCCGCCATCCCACCTATGATGTACCCC
                    ***** *** *****************..    ***************************

HsCD00352083        CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
NM_009587.2         CACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAG
NM_001040078.2      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
XM_005256769.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
XM_005256770.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
NM_001042685.1      CACCCTGCCTATCCGATGCCTTTCATCACCACCATTCCGGGAGGGCTGTACCCATCCAAG
                    ***** ******************************* **********************

HsCD00352083        TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_009587.2         TCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_001040078.2      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
XM_005256769.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
XM_005256770.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
NM_001042685.1      TCCATCATCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATCAACCTGTGC
                    ******.*****************************************************

HsCD00352083        TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
NM_009587.2         TCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGC
NM_001040078.2      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
XM_005256769.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
XM_005256770.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
NM_001042685.1      TCTGGGAGCCACATCGCCTTCCACATGAACCCCCGTTTTGATGAGAATGCTGTGGTCCGT
                    *******.****************.********************************** 

HsCD00352083        AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_009587.2         AACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_001040078.2      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
XM_005256769.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
XM_005256770.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
NM_001042685.1      AACACCCAGATCAACAACTCTTGGGGGTCTGAGGAGCGAAGTCTGCCCCGAAAAATGCCC
                    ************.******* ***************************************

HsCD00352083        TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_009587.2         TTCGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_001040078.2      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
XM_005256769.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
XM_005256770.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
NM_001042685.1      TTCGTCCGAGGCCAGAGCTTCTCGGTGTGGATCTTGTGTGAAGCTCACTGCCTCAAGGTG
                    ********:**************.************************************

HsCD00352083        GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_009587.2         GCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_001040078.2      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
XM_005256769.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
XM_005256770.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
NM_001042685.1      GCCGTGGATGGTCAGCACGTGTTTGAATACTACCATCGCCTGAGGAACCTGCCCACCATC
                    ****************** *****************************************

HsCD00352083        AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACA---
NM_009587.2         AACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATAG
NM_001040078.2      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
XM_005256769.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
XM_005256770.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
NM_001042685.1      AACAAACTGGAAGTGGGTGGCGACATCCAGCTGACCCACGTGCAGACATAG
                    ****.************ ******************** *********   

Clone

Cloning Information : 564690

Organism


TAX_ID : 9606
Species Specific ID: 654346

Genome

Chromosome : 17
Map Location : 17p11.2
Ensembl : ENSG00000171916

Reagents

Labome : LGALS9C-antibody