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MRI1 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MRI1
Gene Name:  methylthioribose-1-phosphate isomerase 1
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100071642)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00352176

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1107nts         Open reading frame : 1 to 1107
Reference Sequence Alignment

ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
GATGGAACCCTAGATGGACCCCAGATG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MRI1
Symbol Nomenclature : MRI1
SYNONYM : MRDI; MTNA; Ypr118w
Designation : M1Pi|MTR-1-P isomerase|S-methyl-5-thioribose-1-phosphate isomerase 1|mediator of RhoA-dependent invasion|methylthioribose-1-phosphate isomerase|methylthioribose-1-phosphate isomerase homolog|translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Full Nomenclature : methylthioribose-1-phosphate isomerase 1
GENEID : 84245
Locus Tag : UNQ6390/PRO21135
GenBank Accession : 564783
HGNC : 28469
MIM : 615105
Vega : OTTHUMG00000180867
Target GenBank: HQ448241

 

Reference Sequence Alignment

  

HsCD00352176        ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
NM_001031727.2      ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
                    ************************************************************

HsCD00352176        CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
NM_001031727.2      CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
                    ************************************************************

HsCD00352176        CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
NM_001031727.2      CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
                    ************************************************************

HsCD00352176        GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
NM_001031727.2      GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
                    ************************************************************

HsCD00352176        GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
NM_001031727.2      GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
                    ************************************************************

HsCD00352176        CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
NM_001031727.2      CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
                    ************************************************************

HsCD00352176        GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
NM_001031727.2      GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
                    ************************************************************

HsCD00352176        CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
NM_001031727.2      CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
                    ************************************************************

HsCD00352176        AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
NM_001031727.2      AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
                    ************************************************************

HsCD00352176        GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
NM_001031727.2      GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
                    ************************************************************

HsCD00352176        GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
NM_001031727.2      GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
                    ************************************************************

HsCD00352176        ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
NM_001031727.2      ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
                    ************************************************************

HsCD00352176        TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
NM_001031727.2      TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
                    ************************************************************

HsCD00352176        GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
NM_001031727.2      GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
                    ************************************************************

HsCD00352176        CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
NM_001031727.2      CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
                    ************************************************************

HsCD00352176        CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
NM_001031727.2      CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
                    ************************************************************

HsCD00352176        TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
NM_001031727.2      TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
                    ************************************************************

HsCD00352176        CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
NM_001031727.2      CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
                    ************************************************************

HsCD00352176        GATGGAACCCTAGATGGACCCCAGATG---
NM_001031727.2      GATGGAACCCTAGATGGACCCCAGATGTAA
                    ***************************   

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Protein

UniProt : Q9BV20
HPRD : 14548

Clone

Cloning Information : 564783

Organism


TAX_ID : 9606
Species Specific ID: 84245

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000037757

Reagents

Labome : MRI1-antibody