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FBXO8 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FBXO8
Gene Name:  F-box protein 8
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100071176)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00352452

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 957nts         Open reading frame : 1 to 957
Reference Sequence Alignment

ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGT
GAGCAAGGCTACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAAC
ACCAATCATCGTAAACAAGTCCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGG
AAATCGAAAGAACAGGAAGGATTCATTAATTTGGAAATGTTGCCTCCTGAGCTAAGCTTT
ACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGGCTTCATGTGTTTGGCAGGAC
CTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGGTCACTGTTCC
ATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT
GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAG
GGTATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTA
AATTGGAAAAAACTGAGAATCTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTA
ACATTGCATAATTTTAGAAATCAGTTCTTGCCAAATGCACTGAGAGAATTTTTTCGTCAT
ATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTCTTATAACAAAGTTCTCACAT
AGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCCTGATGCTGTC
TATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG
AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGT
GAAGATTTTGTAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCTGCA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FBXO8
Symbol Nomenclature : FBXO8
SYNONYM : FBS; FBX8
Designation : F-box only protein 8|F-box protein Fbx8|F-box/SEC7 protein FBS
Full Nomenclature : F-box protein 8
GENEID : 26269
Locus Tag : DC10
GenBank Accession : 565059
HGNC : 13587
MIM : 605649
Vega : OTTHUMG00000160746
Target GenBank: HQ447833

 

Reference Sequence Alignment

  

HsCD00352452      ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGT
NM_012180.2       ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGT
                  ************************************************************

HsCD00352452      GAGCAAGGCTACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAAC
NM_012180.2       GAGCAAGGCTACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAAC
                  ************************************************************

HsCD00352452      ACCAATCATCGTAAACAAGTCCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGG
NM_012180.2       ACCAATCATCGTAAACAAGTCCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGG
                  ************************************************************

HsCD00352452      AAATCGAAAGAACAGGAAGGATTCATTAATTTGGAAATGTTGCCTCCTGAGCTAAGCTTT
NM_012180.2       AAATCGAAAGAACAGGAAGGATTCATTAATTTGGAAATGTTGCCTCCTGAGCTAAGCTTT
                  ************************************************************

HsCD00352452      ACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGGCTTCATGTGTTTGGCAGGAC
NM_012180.2       ACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGGCTTCATGTGTTTGGCAGGAC
                  ************************************************************

HsCD00352452      CTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGGTCACTGTTCC
NM_012180.2       CTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGGTCACTGTTCC
                  ************************************************************

HsCD00352452      ATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT
NM_012180.2       ATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT
                  ************************************************************

HsCD00352452      GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAG
NM_012180.2       GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAG
                  ************************************************************

HsCD00352452      GGTATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTA
NM_012180.2       GGTATCCTGGATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTA
                  ************************************************************

HsCD00352452      AATTGGAAAAAACTGAGAATCTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTA
NM_012180.2       AATTGGAAAAAACTGAGAATCTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTA
                  ************************************************************

HsCD00352452      ACATTGCATAATTTTAGAAATCAGTTCTTGCCAAATGCACTGAGAGAATTTTTTCGTCAT
NM_012180.2       ACATTGCATAATTTTAGAAATCAGTTCTTGCCAAATGCACTGAGAGAATTTTTTCGTCAT
                  ************************************************************

HsCD00352452      ATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTCTTATAACAAAGTTCTCACAT
NM_012180.2       ATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTCTTATAACAAAGTTCTCACAT
                  ************************************************************

HsCD00352452      AGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCCTGATGCTGTC
NM_012180.2       AGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCCTGATGCTGTC
                  ************************************************************

HsCD00352452      TATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG
NM_012180.2       TATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG
                  ************************************************************

HsCD00352452      AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGT
NM_012180.2       AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGT
                  ************************************************************

HsCD00352452      GAAGATTTTGTAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCTGCA---
NM_012180.2       GAAGATTTTGTAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCTGCATAA
                  *********************************************************   

Pathways

NCI : Arf6 signaling events

Protein

SMART domain : Sec7 : Sec7 domain
UniProt : Q9NRD0
UniProt : Q8IXA8
HPRD : 07293

Clone

Cloning Information : 565059

Organism


TAX_ID : 9606
Species Specific ID: 26269

Genome

Chromosome : 4
Map Location : 4q34.1
Ensembl : ENSG00000164117

Reagents

Labome : FBXO8-antibody