DNASU Plasmid Repository • 480.965.5697 | Email

SOCS4 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  SOCS4
Gene Name:  suppressor of cytokine signaling 4
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100071950)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00352938

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1320nts         Open reading frame : 1 to 1320
Reference Sequence Alignment

ATGGCAGAAAATAATGAAAATATTAGTAAAAATGTAGATGTAAGGCCCAAAACTAGTCGG
AGCAGAAGTGCCGACAGAAAAGACGGTTATGTGTGGAGTGGAAAGAAGTTATCTTGGTCA
AAAAAGAGTGAGAGTTATTCAGATGCTGAGACAGTGAATGGTATAGAGAAAACCGAAGTG
TCTTTAAGGAACCAAGAAAGGAAGCACAGCTGTTCATCCATTGAGTTGGACTTAGATCAT
TCCTGTGGGCATCGATTTTTAGGCCGATCTCTTAAACAGAAACTGCAAGATGCCGTGGGG
CAGTGTTTTCCAATAAAGAATTGTAGTAGTCGGCACTCTTCAGGGCTTCCGTCTAAAAGG
AAAATTCATATCAGTGAACTCATGTTAGATAAGTGTCCTTTCCCACCTCGATCAGATTTA
GCCTTTAGGTGGCATTTTATTAAACGACACACTGCTCCTATAAATTCCAAATCAGATGAA
TGGGTAAGCACAGACTTGTCTCAGACTGAATTGAGGGATGGTCAGCTAAAACGAAGAAAT
ATGGAAGAAAATATAAACTGTTTCTCACATACCAATGTTCAGCCCTGTGTCATAACCACC
GACAATGCTTTGTGTAGAGAAGGTCCTATGACTGGCTCTGTGATGAACCTGGTTTCAAAT
AACAGTATAGAAGATAGTGATATGGATTCCGATGATGAAATTCTAACACTTTGCACAAGT
TCCAGAAAAAGAAACAAACCCAAATGGGATTTGGATGATGAAATCCTGCAGTTGGAAACA
CCTCCTAAATACCACACGCAGATTGATTATGTCCACTGTCTTGTACCAGACCTCCTTCAG
ATCAATAACAACCCATGTTACTGGGGAGTGATGGATAAATACGCAGCCGAAGCACTACTG
GAAGGAAAACCAGAGGGTACCTTTTTACTTCGAGACTCAGCACAGGAAGACTATTTATTC
TCTGTTAGTTTTAGACGCTATAGTCGTTCTCTTCATGCTAGAATTGAACAGTGGAATCAC
AACTTTAGCTTTGATGCACATGACCCCTGTGTCTTCCATTCTCCTGACATTACTGGGCTC
CTAGAACATTATAAGGACCCAAGCGCCTGTATGTTCTTTGAACCACTTCTATCCACTCCC
TTAATTCGGACTTTCCCTTTTTCCCTGCAGCATATATGCAGAACAGTTATTTGTAACTGT
ACAACTTATGATGGCATCGATGCCCTTCCAATTCCTTCTTCTATGAAATTATATCTGAAG
GAATATCATTATAAATCAAAAGTTAGAGTACTCAGGATTGATGCACCAGAACAGCAATGC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SOCS4
Symbol Nomenclature : SOCS4
SYNONYM : SOCS7
Designation : SH2 domain containing SOCS box protein|SOCS-4|SOCS-7|suppressor of cytokine signaling 7
Full Nomenclature : suppressor of cytokine signaling 4
GENEID : 122809
GenBank Accession : 565545
HGNC : 19392
Vega : OTTHUMG00000140311
Target GenBank: HQ448516

 

Reference Sequence Alignment

  

HsCD00352938      ATGGCAGAAAATAATGAAAATATTAGTAAAAATGTAGATGTAAGGCCCAAAACTAGTCGG
NM_080867.2       ATGGCAGAAAATAATGAAAATATTAGTAAAAATGTAGATGTAAGGCCCAAAACTAGTCGG
NM_199421.1       ATGGCAGAAAATAATGAAAATATTAGTAAAAATGTAGATGTAAGGCCCAAAACTAGTCGG
                  ************************************************************

HsCD00352938      AGCAGAAGTGCCGACAGAAAAGACGGTTATGTGTGGAGTGGAAAGAAGTTATCTTGGTCA
NM_080867.2       AGCAGAAGTGCCGACAGAAAAGACGGTTATGTGTGGAGTGGAAAGAAGTTATCTTGGTCA
NM_199421.1       AGCAGAAGTGCCGACAGAAAAGACGGTTATGTGTGGAGTGGAAAGAAGTTATCTTGGTCA
                  ************************************************************

HsCD00352938      AAAAAGAGTGAGAGTTATTCAGATGCTGAGACAGTGAATGGTATAGAGAAAACCGAAGTG
NM_080867.2       AAAAAGAGTGAGAGTTATTCAGATGCTGAGACAGTGAATGGTATAGAGAAAACCGAAGTG
NM_199421.1       AAAAAGAGTGAGAGTTATTCAGATGCTGAGACAGTGAATGGTATAGAGAAAACCGAAGTG
                  ************************************************************

HsCD00352938      TCTTTAAGGAACCAAGAAAGGAAGCACAGCTGTTCATCCATTGAGTTGGACTTAGATCAT
NM_080867.2       TCTTTAAGGAACCAAGAAAGGAAGCACAGCTGTTCATCCATTGAGTTGGACTTAGATCAT
NM_199421.1       TCTTTAAGGAACCAAGAAAGGAAGCACAGCTGTTCATCCATTGAGTTGGACTTAGATCAT
                  ************************************************************

HsCD00352938      TCCTGTGGGCATCGATTTTTAGGCCGATCTCTTAAACAGAAACTGCAAGATGCCGTGGGG
NM_080867.2       TCCTGTGGGCATCGATTTTTAGGCCGATCTCTTAAACAGAAACTGCAAGATGCCGTGGGG
NM_199421.1       TCCTGTGGGCATCGATTTTTAGGCCGATCTCTTAAACAGAAACTGCAAGATGCCGTGGGG
                  ************************************************************

HsCD00352938      CAGTGTTTTCCAATAAAGAATTGTAGTAGTCGGCACTCTTCAGGGCTTCCGTCTAAAAGG
NM_080867.2       CAGTGTTTTCCAATAAAGAATTGTAGTAGTCGGCACTCTTCAGGGCTTCCGTCTAAAAGG
NM_199421.1       CAGTGTTTTCCAATAAAGAATTGTAGTAGTCGGCACTCTTCAGGGCTTCCGTCTAAAAGG
                  ************************************************************

HsCD00352938      AAAATTCATATCAGTGAACTCATGTTAGATAAGTGTCCTTTCCCACCTCGATCAGATTTA
NM_080867.2       AAAATTCATATCAGTGAACTCATGTTAGATAAGTGTCCTTTCCCACCTCGATCAGATTTA
NM_199421.1       AAAATTCATATCAGTGAACTCATGTTAGATAAGTGTCCTTTCCCACCTCGATCAGATTTA
                  ************************************************************

HsCD00352938      GCCTTTAGGTGGCATTTTATTAAACGACACACTGCTCCTATAAATTCCAAATCAGATGAA
NM_080867.2       GCCTTTAGGTGGCATTTTATTAAACGACACACTGCTCCTATAAATTCCAAATCAGATGAA
NM_199421.1       GCCTTTAGGTGGCATTTTATTAAACGACACACTGCTCCTATAAATTCCAAATCAGATGAA
                  ************************************************************

HsCD00352938      TGGGTAAGCACAGACTTGTCTCAGACTGAATTGAGGGATGGTCAGCTAAAACGAAGAAAT
NM_080867.2       TGGGTAAGCACAGACTTGTCTCAGACTGAATTGAGGGATGGTCAGCTAAAACGAAGAAAT
NM_199421.1       TGGGTAAGCACAGACTTGTCTCAGACTGAATTGAGGGATGGTCAGCTAAAACGAAGAAAT
                  ************************************************************

HsCD00352938      ATGGAAGAAAATATAAACTGTTTCTCACATACCAATGTTCAGCCCTGTGTCATAACCACC
NM_080867.2       ATGGAAGAAAATATAAACTGTTTCTCACATACCAATGTTCAGCCCTGTGTCATAACCACC
NM_199421.1       ATGGAAGAAAATATAAACTGTTTCTCACATACCAATGTTCAGCCCTGTGTCATAACCACC
                  ************************************************************

HsCD00352938      GACAATGCTTTGTGTAGAGAAGGTCCTATGACTGGCTCTGTGATGAACCTGGTTTCAAAT
NM_080867.2       GACAATGCTTTGTGTAGAGAAGGTCCTATGACTGGCTCTGTGATGAACCTGGTTTCAAAT
NM_199421.1       GACAATGCTTTGTGTAGAGAAGGTCCTATGACTGGCTCTGTGATGAACCTGGTTTCAAAT
                  ************************************************************

HsCD00352938      AACAGTATAGAAGATAGTGATATGGATTCCGATGATGAAATTCTAACACTTTGCACAAGT
NM_080867.2       AACAGTATAGAAGATAGTGATATGGATTCCGATGATGAAATTCTAACACTTTGCACAAGT
NM_199421.1       AACAGTATAGAAGATAGTGATATGGATTCCGATGATGAAATTCTAACACTTTGCACAAGT
                  ************************************************************

HsCD00352938      TCCAGAAAAAGAAACAAACCCAAATGGGATTTGGATGATGAAATCCTGCAGTTGGAAACA
NM_080867.2       TCCAGAAAAAGAAACAAACCCAAATGGGATTTGGATGATGAAATCCTGCAGTTGGAAACA
NM_199421.1       TCCAGAAAAAGAAACAAACCCAAATGGGATTTGGATGATGAAATCCTGCAGTTGGAAACA
                  ************************************************************

HsCD00352938      CCTCCTAAATACCACACGCAGATTGATTATGTCCACTGTCTTGTACCAGACCTCCTTCAG
NM_080867.2       CCTCCTAAATACCACACGCAGATTGATTATGTCCACTGTCTTGTACCAGACCTCCTTCAG
NM_199421.1       CCTCCTAAATACCACACGCAGATTGATTATGTCCACTGTCTTGTACCAGACCTCCTTCAG
                  ************************************************************

HsCD00352938      ATCAATAACAACCCATGTTACTGGGGAGTGATGGATAAATACGCAGCCGAAGCACTACTG
NM_080867.2       ATCAATAACAACCCATGTTACTGGGGAGTGATGGATAAATACGCAGCCGAAGCACTACTG
NM_199421.1       ATCAATAACAACCCATGTTACTGGGGAGTGATGGATAAATACGCAGCCGAAGCACTACTG
                  ************************************************************

HsCD00352938      GAAGGAAAACCAGAGGGTACCTTTTTACTTCGAGACTCAGCACAGGAAGACTATTTATTC
NM_080867.2       GAAGGAAAACCAGAGGGTACCTTTTTACTTCGAGACTCAGCACAGGAAGACTATTTATTC
NM_199421.1       GAAGGAAAACCAGAGGGTACCTTTTTACTTCGAGACTCAGCACAGGAAGACTATTTATTC
                  ************************************************************

HsCD00352938      TCTGTTAGTTTTAGACGCTATAGTCGTTCTCTTCATGCTAGAATTGAACAGTGGAATCAC
NM_080867.2       TCTGTTAGTTTTAGACGCTATAGTCGTTCTCTTCATGCTAGAATTGAACAGTGGAATCAC
NM_199421.1       TCTGTTAGTTTTAGACGCTATAGTCGTTCTCTTCATGCTAGAATTGAACAGTGGAATCAC
                  ************************************************************

HsCD00352938      AACTTTAGCTTTGATGCACATGACCCCTGTGTCTTCCATTCTCCTGACATTACTGGGCTC
NM_080867.2       AACTTTAGCTTTGATGCACATGACCCCTGTGTCTTCCATTCTCCTGACATTACTGGGCTC
NM_199421.1       AACTTTAGCTTTGATGCACATGACCCCTGTGTCTTCCATTCTCCTGACATTACTGGGCTC
                  ************************************************************

HsCD00352938      CTAGAACATTATAAGGACCCAAGCGCCTGTATGTTCTTTGAACCACTTCTATCCACTCCC
NM_080867.2       CTAGAACATTATAAGGACCCAAGCGCCTGTATGTTCTTTGAACCACTTCTATCCACTCCC
NM_199421.1       CTAGAACATTATAAGGACCCAAGCGCCTGTATGTTCTTTGAACCACTTCTATCCACTCCC
                  ************************************************************

HsCD00352938      TTAATTCGGACTTTCCCTTTTTCCCTGCAGCATATATGCAGAACAGTTATTTGTAACTGT
NM_080867.2       TTAATTCGGACTTTCCCTTTTTCCCTGCAGCATATATGCAGAACAGTTATTTGTAACTGT
NM_199421.1       TTAATTCGGACTTTCCCTTTTTCCCTGCAGCATATATGCAGAACAGTTATTTGTAACTGT
                  ************************************************************

HsCD00352938      ACAACTTATGATGGCATCGATGCCCTTCCAATTCCTTCTTCTATGAAATTATATCTGAAG
NM_080867.2       ACAACTTATGATGGCATCGATGCCCTTCCAATTCCTTCTTCTATGAAATTATATCTGAAG
NM_199421.1       ACAACTTATGATGGCATCGATGCCCTTCCAATTCCTTCTTCTATGAAATTATATCTGAAG
                  ************************************************************

HsCD00352938      GAATATCATTATAAATCAAAAGTTAGAGTACTCAGGATTGATGCACCAGAACAGCAATGC
NM_080867.2       GAATATCATTATAAATCAAAAGTTAGAGTACTCAGGATTGATGCACCAGAACAGCAATGC
NM_199421.1       GAATATCATTATAAATCAAAAGTTAGAGTACTCAGGATTGATGCACCAGAACAGCAATGC
                  ************************************************************

HsCD00352938      ---
NM_080867.2       TAG
NM_199421.1       TAG
                     

Pathways

Panther : Inflammation mediated by chemokine and cytokine signaling pathway
Panther : Interferon-gamma signaling pathway
WikiPathway : Type II diabetes mellitus

Protein

SMART domain : SH2 : Src homology 2 domains
SMART domain : SOCS : suppressors of cytokine signalling
SMART domain : SOCS_box :
UniProt : Q8WXH5
UniProt : Q5H9R6
HPRD : 18084

Clone

Cloning Information : 565545

Organism


TAX_ID : 9606
Species Specific ID: 122809

Genome

Chromosome : 14
Map Location : 14q22.1
Ensembl : ENSG00000180008

Reagents

Labome : SOCS4-antibody