DNASU Plasmid Repository • 480.965.5697 | Email

RAMP3 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  RAMP3
Gene Name:  receptor (G protein-coupled) activity modifying protein 3
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072023)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00353036

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 444nts         Open reading frame : 1 to 444
Reference Sequence Alignment

ATGGAGACTGGAGCGCTGCGGCGCCCGCAACTTCTCCCGTTGCTGCTGCTGCTCTGCGGT
GGGTGTCCCAGAGCAGGCGGCTGCAACGAGACAGGCCTGTTGGAGAGGCTGCCCCTGTGT
GGGAAGGCTTTCGCAGACATGATGGGCAAGGTGGACGTCTGGAAGTGGTGCAACCTGTCC
GAGTTCATCGTGTACTATGAGAGTTTCACCAACTGCACCGAGATGGAGGCCAATGTCGTG
GGCTGCTACTGGCCCAACCCCCTGGCCCAGGGCTTCATCACCGGCATCCACAGGCAGTTC
TTCTCCAACTGCACCGTGGACAGGGTCCACTTGGAGGACCCCCCAGACGAGGTTCTCATC
CCGCTGATCGTTATACCCGTCGTTCTGACTGTCGCCATGGCTGGCCTGGTGGTGTGGCGC
AGCAAACGCACCGACACGCTGCTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RAMP3
Symbol Nomenclature : RAMP3
Designation : CRLR activity-modifying protein 3|calcitonin receptor-like receptor activity modifying protein 3|calcitonin-receptor-like receptor activity-modifying protein 3|receptor (calcitonin) activity modifying protein 3|receptor activity modifying protein 3|receptor activity-modifying protein 3|receptor-activity-modifying protein 3
Full Nomenclature : receptor (G protein-coupled) activity modifying protein 3
GENEID : 10268
GenBank Accession : 565643
HGNC : 9845
MIM : 605155
Vega : OTTHUMG00000023729
Target GenBank: HQ448585

 

Reference Sequence Alignment

  

HsCD00353036      ATGGAGACTGGAGCGCTGCGGCGCCCGCAACTTCTCCCGTTGCTGCTGCTGCTCTGCGGT
NM_005856.2       ATGGAGACTGGAGCGCTGCGGCGCCCGCAACTTCTCCCGTTGCTGCTGCTGCTCTGCGGT
                  ************************************************************

HsCD00353036      GGGTGTCCCAGAGCAGGCGGCTGCAACGAGACAGGCCTGTTGGAGAGGCTGCCCCTGTGT
NM_005856.2       GGGTGTCCCAGAGCAGGCGGCTGCAACGAGACAGGCATGTTGGAGAGGCTGCCCCTGTGT
                  ************************************.***********************

HsCD00353036      GGGAAGGCTTTCGCAGACATGATGGGCAAGGTGGACGTCTGGAAGTGGTGCAACCTGTCC
NM_005856.2       GGGAAGGCTTTCGCAGACATGATGGGCAAGGTGGACGTCTGGAAGTGGTGCAACCTGTCC
                  ************************************************************

HsCD00353036      GAGTTCATCGTGTACTATGAGAGTTTCACCAACTGCACCGAGATGGAGGCCAATGTCGTG
NM_005856.2       GAGTTCATCGTGTACTATGAGAGTTTCACCAACTGCACCGAGATGGAGGCCAATGTCGTG
                  ************************************************************

HsCD00353036      GGCTGCTACTGGCCCAACCCCCTGGCCCAGGGCTTCATCACCGGCATCCACAGGCAGTTC
NM_005856.2       GGCTGCTACTGGCCCAACCCCCTGGCCCAGGGCTTCATCACCGGCATCCACAGGCAGTTC
                  ************************************************************

HsCD00353036      TTCTCCAACTGCACCGTGGACAGGGTCCACTTGGAGGACCCCCCAGACGAGGTTCTCATC
NM_005856.2       TTCTCCAACTGCACCGTGGACAGGGTCCACTTGGAGGACCCCCCAGACGAGGTTCTCATC
                  ************************************************************

HsCD00353036      CCGCTGATCGTTATACCCGTCGTTCTGACTGTCGCCATGGCTGGCCTGGTGGTGTGGCGC
NM_005856.2       CCGCTGATCGTTATACCCGTCGTTCTGACTGTCGCCATGGCTGGCCTGGTGGTGTGGCGC
                  ************************************************************

HsCD00353036      AGCAAACGCACCGACACGCTGCTG---
NM_005856.2       AGCAAACGCACCGACACGCTGCTGTGA
                  ************************   

Pathways

Reactome : Calcitonin-like ligand receptors
Reactome : Class B/2 (Secretin family receptors)
Reactome : G alpha (s) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : Myometrial Relaxation and Contraction Pathways

Protein

UniProt : A4D2L1
UniProt : O60896
HPRD : 05517

Clone

Cloning Information : 565643

Organism


TAX_ID : 9606
Species Specific ID: 10268

Genome

Chromosome : 7
Map Location : 7p13-p12
Ensembl : ENSG00000122679

Reagents

Labome : RAMP3-antibody