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LYSMD1 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LYSMD1
Gene Name:  LysM, putative peptidoglycan-binding, domain containing 1
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100070780)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00353161

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 681nts        
Open reading frame : 1 to 681
Reference Sequence Alignment

ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
GAGGATGAAATCTTCAAACTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LYSMD1
Symbol Nomenclature : LYSMD1
SYNONYM : RP11-68I18.5
Designation : lysM and putative peptidoglycan-binding domain-containing protein 1
Full Nomenclature : LysM, putative peptidoglycan-binding, domain containing 1
GENEID : 388695
Locus Tag : SB145
GenBank Accession : 565768
HGNC : 32070
Vega : OTTHUMG00000012260
Target GenBank: HQ447451

 

Reference Sequence Alignment

  

HsCD00353161      ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
NM_212551.4       ATGGCTTCCCCGTCTAGACAGCCCCCGCCAGGGGGGTCAGGACTGCTTCAAGGGAGCCGG
                  ************************************************************

HsCD00353161      GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
NM_212551.4       GCTCGTTCATATGGAAGCCTGGTGCAATCGGCCTGCTCCCCAGTGAGGGAAAGACGCCTG
                  ************************************************************

HsCD00353161      GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
NM_212551.4       GAGCATCAGTTGGAGCCCGGAGACACCCTGGCTGGACTAGCACTCAAATATGGGGTGACG
                  ************************************************************

HsCD00353161      ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
NM_212551.4       ATGGAACAGATTAAACGTGCAAACCGCCTTTATACTAATGACTCCATCTTCCTGAAGAAA
                  ************************************************************

HsCD00353161      ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
NM_212551.4       ACCCTCTACATCCCCATCCTGACAGAGCCCAGAGACCTGTTCAATGGTTTGGACTCTGAG
                  ************************************************************

HsCD00353161      GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
NM_212551.4       GAAGAGAAAGATGGAGAGGAAAAAGTACACCCAAGTAACAGTGAAGTTTGGCCACACTCA
                  ************************************************************

HsCD00353161      ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
NM_212551.4       ACTGAGAGGAAGAAACAAGAGACAGGAGCAGGACGTGCCAATGGTGAAGTCCTCCCCACA
                  ************************************************************

HsCD00353161      CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
NM_212551.4       CCTGGCCAGGAAACCCCCACGCCCATCCATGACCTCTCTGCCTCTGATTTCCTTAAGAAG
                  ************************************************************

HsCD00353161      CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
NM_212551.4       CTTGATTCACAGATCAGCCTGTCCAAGAAGGCTGCTGCTCAGAAGCTGAAAAAAGGGGAA
                  ************************************************************

HsCD00353161      AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
NM_212551.4       AATGGGGTACCTGGGGAGGATGCAGGTCTCCACCTGAGCTCCCCTTGGATGCAGCAACGA
                  ************************************************************

HsCD00353161      GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
NM_212551.4       GCAGTCCTAGGTCCTGTGCCGCTGACCCGTACCTCTCGGACCCGGACACTACGGGACCAG
                  ************************************************************

HsCD00353161      GAGGATGAAATCTTCAAACTC---
NM_212551.4       GAGGATGAAATCTTCAAACTCTGA
                  *********************   

Protein

SMART domain : LysM : Lysin motif
UniProt : Q96S90
HPRD : 18017

Clone

Cloning Information : 565768

Organism


TAX_ID : 9606
Species Specific ID: 388695

Genome

Chromosome : 1
Map Location : 1q21.3
Ensembl : ENSG00000163155

Reagents

Labome : LYSMD1-antibody