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OPN5 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN5
Gene Name:  opsin 5
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072508)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00353588

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1062nts         Open reading frame : 1 to 1062
Reference Sequence Alignment

ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN5
Symbol Nomenclature : OPN5
SYNONYM : GPR136; PGR12; TMEM13
Designation : G-protein coupled receptor 136|neuropsin|opsin-5|transmembrane protein 13
Full Nomenclature : opsin 5
GENEID : 221391
GenBank Accession : 571590
HGNC : 19992
MIM : 609042
Target GenBank: HQ258199

 

Reference Sequence Alignment

  

HsCD00353588      ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
NM_181744.3       ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
                  ************************************************************

HsCD00353588      GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
NM_181744.3       GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
                  ************************************************************

HsCD00353588      ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
NM_181744.3       ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
                  ************************************************************

HsCD00353588      CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
NM_181744.3       CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
                  ************************************************************

HsCD00353588      GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
NM_181744.3       GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
                  ************************************************************

HsCD00353588      TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
NM_181744.3       TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
                  ************************************************************

HsCD00353588      CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
NM_181744.3       CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
                  ************************************************************

HsCD00353588      GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
NM_181744.3       GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
                  ************************************************************

HsCD00353588      TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
NM_181744.3       TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
                  ************************************************************

HsCD00353588      ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
NM_181744.3       ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
                  ************************************************************

HsCD00353588      CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
NM_181744.3       CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
                  ************************************************************

HsCD00353588      AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
NM_181744.3       AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
                  ************************************************************

HsCD00353588      CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
NM_181744.3       CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
                  ************************************************************

HsCD00353588      TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
NM_181744.3       TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
                  ************************************************************

HsCD00353588      GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
NM_181744.3       GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
                  ************************************************************

HsCD00353588      TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
NM_181744.3       TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
                  ************************************************************

HsCD00353588      TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
NM_181744.3       TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
                  ************************************************************

HsCD00353588      AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA---
NM_181744.3       AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAATAA
                  ******************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR

Protein

UniProt : Q6U736
HPRD : 12358

Clone

Cloning Information : 571590

Organism


TAX_ID : 9606
Species Specific ID: 221391

Genome

Chromosome : 6
Map Location : 6p12.3

Reagents

Labome : OPN5-antibody