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GABRR1 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GABRR1
Gene Name:  gamma-aminobutyric acid (GABA) A receptor, rho 1
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072570)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00353609

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1422nts         Open reading frame : 1 to 1422
Reference Sequence Alignment

ATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTGGCCACTGAAAGCAGAATG
CACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAGGCAGTAGGCCCCAAAGACAA
AGACGAGAAGTACATGAAGATGCCCACAAGCAAGTCAGCCCAATTCTGAGACGAAGTCCT
GACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTGAGGATAGATGACCATGAT
TTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTTGGTGTGGATGTGCAGGTG
GAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACGATGACCCTCTACCTGAGG
CACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAACAACCTCAGCATGACGTTT
GATGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTTTTCGTGCACTCCAAACGC
TCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGGGTCCAGCCTGATGGGAAA
GTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAACATGGACTTCAGCCGATTT
CCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTATGCCTATACAGAAGATGAC
CTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACAGATGAACGGATCTCACTC
TCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTGGCTTTCTACAGCAGCACA
GGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGCCACATCTTCTTCTTCTTG
CTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCCTGGGTGTCCTTCTGGATC
GACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACAACGGTGCTGACCATGTCC
ACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCCTACATCAAGGCCGTGGAC
ATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTGCTGGAGTATGCGGCCGTC
AACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTGCGGGAGAAGCTTCCCTGC
ACCAGCGGATTACCTCCGCCCCGCACTGCAATGCTGGACGGCAACTACAGTGATGGGGAG
GTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAGCCCGACAGGATGATGGTG
CAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAAAGTCAGAGAAGCAGCTAT
GTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCCAGGATCATCTTTCCAGCA
GCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GABRR1
Symbol Nomenclature : GABRR1
Designation : GABA(A) receptor subunit rho-1|GABA(A) receptor, rho 1|GABA(C) receptor|bA135P14.1 (gamma-aminobutyric acid (GABA) receptor, rho 1)|gamma-aminobutyric acid (GABA) A receptor, rho-1|gamma-aminobutyric acid (GABA) receptor, rho 1|gamma-aminobutyric acid receptor subunit rho-1
Full Nomenclature : gamma-aminobutyric acid (GABA) A receptor, rho 1
GENEID : 2569
Locus Tag : RP11-63L7.1
GenBank Accession : 571611
HGNC : 4090
MIM : 137161
Vega : OTTHUMG00000015195
Target GenBank: HQ258261

 

Reference Sequence Alignment

  

HsCD00353609        ------------------ATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTG
NM_002042.4         ATGTTGGCTGTCCCAAATATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTG
NM_001256703.1      ATGTTGGCTGTCCCAAATATGAGATTTGGCATCTTTCTTTTGTGGTGGGGATGGGTTTTG
                                      ******************************************

HsCD00353609        GCCACTGAAAGCAGAATGCACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAGGC
NM_002042.4         GCCACTGAAAGCAGAATGCACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAG--
NM_001256703.1      GCCACTGAAAGCAGAATGCACTGGCCCGGAAGAGAAGTCCACGAGATGTCTAAGAAAG--
                    **********************************************************  

HsCD00353609        AGTAGGCCCCAAAGACAAAGACGAGAAGTACATGAAGATGCCCACAAGCAAGTCAGCCCA
NM_002042.4         -GCAGGCCCCAAAGACAAAGACGAGAAGTACATGAAGATGCCCACAAGCAAGTCAGCCCA
NM_001256703.1      -GCAG---------------------------------------------------CCCA
                     * **                                                   ****

HsCD00353609        ATTCTGAGACGAAGTCCTGACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTG
NM_002042.4         ATTCTGAGACGAAGTCCTGACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTG
NM_001256703.1      ATTCTGAGACGAAGTCCTGACATCACCAAATCGCCTCTGACAAAGTCAGAACAGCTTCTG
                    ************************************************************

HsCD00353609        AGGATAGATGACCATGATTTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTT
NM_002042.4         AGGATAGATGACCATGATTTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTT
NM_001256703.1      AGGATAGATGACCATGATTTCAGCATGAGGCCTGGCTTTGGAGGCCCTGCCATTCCTGTT
                    ************************************************************

HsCD00353609        GGTGTGGATGTGCAGGTGGAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACG
NM_002042.4         GGTGTGGATGTGCAGGTGGAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACG
NM_001256703.1      GGTGTGGATGTGCAGGTGGAGAGTTTGGATAGCATCTCAGAGGTTGACATGGACTTTACG
                    ************************************************************

HsCD00353609        ATGACCCTCTACCTGAGGCACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAAC
NM_002042.4         ATGACCCTCTACCTGAGGCACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAAC
NM_001256703.1      ATGACCCTCTACCTGAGGCACTACTGGAAGGACGAGAGGCTGTCTTTTCCAAGCACCAAC
                    ************************************************************

HsCD00353609        AACCTCAGCATGACGTTTGATGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTT
NM_002042.4         AACCTCAGCATGACGTTTGACGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTT
NM_001256703.1      AACCTCAGCATGACGTTTGACGGCCGGCTGGTCAAGAAGATCTGGGTCCCTGACATGTTT
                    ******************** ***************************************

HsCD00353609        TTCGTGCACTCCAAACGCTCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGG
NM_002042.4         TTCGTGCACTCCAAACGCTCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGG
NM_001256703.1      TTCGTGCACTCCAAACGCTCCTTCATCCACGACACCACCACAGACAACGTCATGTTGCGG
                    ************************************************************

HsCD00353609        GTCCAGCCTGATGGGAAAGTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAAC
NM_002042.4         GTCCAGCCTGATGGGAAAGTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAAC
NM_001256703.1      GTCCAGCCTGATGGGAAAGTGCTCTATAGTCTCAGGGTTACAGTAACTGCAATGTGCAAC
                    ************************************************************

HsCD00353609        ATGGACTTCAGCCGATTTCCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTAT
NM_002042.4         ATGGACTTCAGCCGATTTCCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTAT
NM_001256703.1      ATGGACTTCAGCCGATTTCCCTTGGACACACAAACGTGCTCTCTTGAAATTGAAAGCTAT
                    ************************************************************

HsCD00353609        GCCTATACAGAAGATGACCTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACA
NM_002042.4         GCCTATACAGAAGATGACCTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACA
NM_001256703.1      GCCTATACAGAAGATGACCTCATGCTGTACTGGAAAAAGGGCAATGACTCCTTAAAGACA
                    ************************************************************

HsCD00353609        GATGAACGGATCTCACTCTCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTG
NM_002042.4         GATGAACGGATCTCACTCTCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTG
NM_001256703.1      GATGAACGGATCTCACTCTCCCAGTTCCTCATTCAGGAATTCCACACCACCACCAAACTG
                    ************************************************************

HsCD00353609        GCTTTCTACAGCAGCACAGGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGC
NM_002042.4         GCTTTCTACAGCAGCACAGGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGC
NM_001256703.1      GCTTTCTACAGCAGCACAGGCTGGTACAACCGTCTCTACATTAATTTCACGTTGCGTCGC
                    ************************************************************

HsCD00353609        CACATCTTCTTCTTCTTGCTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCC
NM_002042.4         CACATCTTCTTCTTCTTGCTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCC
NM_001256703.1      CACATCTTCTTCTTCTTGCTCCAAACTTATTTCCCCGCTACCCTGATGGTCATGCTGTCC
                    ************************************************************

HsCD00353609        TGGGTGTCCTTCTGGATCGACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACA
NM_002042.4         TGGGTGTCCTTCTGGATCGACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACA
NM_001256703.1      TGGGTGTCCTTCTGGATCGACCGCAGAGCCGTGCCTGCCAGAGTCCCCTTAGGTATCACA
                    ************************************************************

HsCD00353609        ACGGTGCTGACCATGTCCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCC
NM_002042.4         ACGGTGCTGACCATGTCCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCC
NM_001256703.1      ACGGTGCTGACCATGTCCACCATCATCACGGGCGTGAATGCCTCCATGCCGCGCGTCTCC
                    ************************************************************

HsCD00353609        TACATCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTG
NM_002042.4         TACATCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTG
NM_001256703.1      TACATCAAGGCCGTGGACATCTACCTCTGGGTCAGCTTTGTGTTCGTGTTCCTCTCGGTG
                    ************************************************************

HsCD00353609        CTGGAGTATGCGGCCGTCAACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTG
NM_002042.4         CTGGAGTATGCGGCCGTCAACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTG
NM_001256703.1      CTGGAGTATGCGGCCGTCAACTACCTGACCACTGTGCAGGAGAGGAAGGAACAGAAGCTG
                    ************************************************************

HsCD00353609        CGGGAGAAGCTTCCCTGCACCAGCGGATTACCTCCGCCCCGCACTGCAATGCTGGACGGC
NM_002042.4         CGGGAGAAGCTTCCCTGCACCAGCGGATTACCTCCGCCCCGCACTGCGATGCTGGACGGC
NM_001256703.1      CGGGAGAAGCTTCCCTGCACCAGCGGATTACCTCCGCCCCGCACTGCGATGCTGGACGGC
                    ***********************************************.************

HsCD00353609        AACTACAGTGATGGGGAGGTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAG
NM_002042.4         AACTACAGTGATGGGGAGGTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAG
NM_001256703.1      AACTACAGTGATGGGGAGGTGAATGACCTGGACAACTACATGCCAGAGAATGGAGAGAAG
                    ************************************************************

HsCD00353609        CCCGACAGGATGATGGTGCAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAA
NM_002042.4         CCCGACAGGATGATGGTGCAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAA
NM_001256703.1      CCCGACAGGATGATGGTGCAGCTGACCCTGGCCTCAGAGAGGAGCTCCCCACAGAGGAAA
                    ************************************************************

HsCD00353609        AGTCAGAGAAGCAGCTATGTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCC
NM_002042.4         AGTCAGAGAAGCAGCTATGTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCC
NM_001256703.1      AGTCAGAGAAGCAGCTATGTGAGCATGAGAATCGACACCCACGCCATTGATAAATACTCC
                    ************************************************************

HsCD00353609        AGGATCATCTTTCCAGCAGCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC
NM_002042.4         AGGATCATCTTTCCAGCAGCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC
NM_001256703.1      AGGATCATCTTTCCAGCAGCATACATTTTATTCAATTTAATATACTGGTCTATTTTCTCC
                    ************************************************************

HsCD00353609        ---
NM_002042.4         TAG
NM_001256703.1      TAG
                       

Pathways

Reactome : GABA A (rho) receptor activation
Reactome : GABA receptor activation
Reactome : Ion channel transport
Reactome : Ligand-gated ion channel transport
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Transmembrane transport of small molecules
Reactome : Transmission across Chemical Synapses

Protein

UniProt : P24046
HPRD : 15921

Clone

Cloning Information : 571611

Organism


TAX_ID : 9606
Species Specific ID: 2569

Genome

Chromosome : 6
Map Location : 6q15
Ensembl : ENSG00000146276

Reagents

Labome : GABRR1-antibody