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C20orf185 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  BPIFB3
Gene Name:  BPI fold containing family B, member 3
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072869)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00353774

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1428nts         Open reading frame : 1 to 1428
Reference Sequence Alignment

ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
ACCAAAGTGGGCATGCATTGCTCTGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
ATGGTGCCACTGTACCTCTTCAACACCATGTTTGGACTCCTGCAGACCAACGGCGCCCTC
GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
AACGTGGCCCTGGATGTTGGAATTTCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB3
Symbol Nomenclature : BPIFB3
SYNONYM : C20orf185; LPLUNC3; RYA3; dJ726C3.4
Designation : BPI fold-containing family B member 3|ligand-binding protein RYA3|long palate, lung and nasal epithelium carcinoma associated 3|long palate, lung and nasal epithelium carcinoma-associated protein 3
Full Nomenclature : BPI fold containing family B, member 3
GENEID : 359710
GenBank Accession : 571776
HGNC : 16178
Vega : OTTHUMG00000032234
Target GenBank: HQ258440

 

Reference Sequence Alignment

  

HsCD00353774      ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
NM_182658.1       ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
                  ************************************************************

HsCD00353774      TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
NM_182658.1       TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
                  ************************************************************

HsCD00353774      AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
NM_182658.1       AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
                  ************************************************************

HsCD00353774      ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
NM_182658.1       ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
                  ************************************************************

HsCD00353774      GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
NM_182658.1       GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
                  ************************************************************

HsCD00353774      ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
NM_182658.1       ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
                  ************************************************************

HsCD00353774      ACCAAAGTGGGCATGCATTGCTCTGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
NM_182658.1       ACCAAAGTGGGCATGCATTGCTCCGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
                  *********************** ************************************

HsCD00353774      GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
NM_182658.1       GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
                  ************************************************************

HsCD00353774      CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
NM_182658.1       CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
                  ************************************************************

HsCD00353774      CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
NM_182658.1       CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
                  ************************************************************

HsCD00353774      GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
NM_182658.1       GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
                  ************************************************************

HsCD00353774      TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
NM_182658.1       TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
                  ************************************************************

HsCD00353774      CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
NM_182658.1       CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
                  ************************************************************

HsCD00353774      TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
NM_182658.1       TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
                  ************************************************************

HsCD00353774      ATGGTGCCACTGTACCTCTTCAACACCATGTTTGGACTCCTGCAGACCAACGGCGCCCTC
NM_182658.1       ATGGTGCCACTGTACCTCTTCAACACCACGTTTGGACTCCTGCAGACCAACGGCGCCCTC
                  **************************** *******************************

HsCD00353774      GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
NM_182658.1       GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
                  ************************************************************

HsCD00353774      GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
NM_182658.1       GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
                  ************************************************************

HsCD00353774      CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
NM_182658.1       CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
                  ************************************************************

HsCD00353774      CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
NM_182658.1       CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
                  ************************************************************

HsCD00353774      CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
NM_182658.1       CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
                  ************************************************************

HsCD00353774      TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
NM_182658.1       TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
                  ************************************************************

HsCD00353774      GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
NM_182658.1       GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
                  ************************************************************

HsCD00353774      AACGTGGCCCTGGATGTTGGAATTTCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
NM_182658.1       AACGTGGCCCTGGATGTTGGAATTCCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
                  ************************ ***********************************

HsCD00353774      TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCC---
NM_182658.1       TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCCTGA
                  ************************************************   

Protein

SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
SMART domain : BPI1 : BPI/LBP/CETP N-terminal domain
UniProt : P59826
HPRD : 16644

Clone

Cloning Information : 571776

Organism


TAX_ID : 9606
Species Specific ID: 359710

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000186190

Reagents

Labome : BPIFB3-antibody