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SRMS (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SRMS
Gene Name:  src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100015619)
Mutation/
Discrepancy :
No / No
Publications: PMID: 23822091
Title: The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1
PMID: 23822091
Title: The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate Dok1
Authors: Center for Personalized Diagnostics
HsCD00356544

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1464nts         Open reading frame : 1 to 1464
Reference Sequence Alignment

ATGGAGCCGTTCCTCAGGAGGCGGCTGGCCTTCCTGTCCTTCTTCTGGGACAAGATCTGG
CCGGCGGGCGGCGAGCCGGACCATGGCACCCCCGGGTCCCTGGACCCCAACACTGACCCA
GTGCCCACGCTCCCCGCCGAGCCTTGCAGCCCCTTCCCTCAGCTCTTCCTTGCGCTCTAT
GACTTCACGGCGCGGTGTGGCGGGGAGCTGAGTGTCCGCCGCGGGGACAGGCTCTGTGCC
CTCGAAGAGGGGGGCGGCTACATCTTCGCACGCAGGCTTTCGGGCCAGCCCAGCGCCGGG
CTCGTGCCCATCACCCACGTGGCCAAGGCTTCTCCTGAGACGCTCTCAGACCAACCCTGG
TACTTTAGCGGGGTCAGTCGGACCCAGGCACAGCAGCTGCTCCTCTCCCCACCCAACGAA
CCAGGGGCCTTCCTCATCCGGCCCAGCGAGAGCAGCCTCGGGGGCTACTCACTGTCAGTC
CGGGCCCAGGCCAAGGTCTGCCACTACCGGGTCTCCATGGCAGCTGATGGCAGCCTCTAC
CTGCAGAAGGGACGGCTCTTTCCCGGCCTGGAGGAGCTGCTCACCTACTACAAGGCCAAC
TGGAAGCTGATCCAGAACCCCCTGCTGCAGCCCTGCATGCCCCAGAAGGCCCCGAGGCAG
GACGTGTGGGAGCGGCCACACTCCGAATTCGCCCTTGGGAGGAAGCTGGGTGAAGGCTAC
TTTGGGGAGGTGTGGGAAGGCCTGTGGCTGGGCTCCCTGCCCGTGGCGATCAAGGTCATC
AAGTCAGCCAACATGAAGCTCACTGACCTCGCCAAGGAGATCCAGACACTGAAGGGCCTG
CGGCACGAGCGGCTCATCCGGCTGCACGCAGTGTGCTCGGGCGGGGAGCCTGTGTACATC
GTCACGGAACTCATGCGCAAGGGGAACCTGCAGGCCTTCCTGGGCACCCCCGAGGGCCGG
GCCCTGCGTCTGCCGCCACTCCTGGGCTTTGCCTGCCAGGTGGCTGAGGGCATGAGCTAC
CTGGAGGAGCAGCGCGTTGTGCACCGGGACTTGGCCGCCCGGAACGTGCTCGTGGACGAC
GGCCTGGCCTGCAAGGTGGCTGACTTCGGCCTGGCCCGGCTGCTCAAGGACGACATCTAC
TCCCCGAGCAGCAGCTCCAAGATCCCGGTCAAGTGGACAGCGCCTGAGGCGGCCAATTAT
CGTGTCTTCTCCCAGAAGTCAGACGTCTGGTCCTTCGGCGTCCTGCTGCACGAGGTTTTC
ACCTATGGCCAGTGTCCCTATGAAGGGATGACCAACCACGAGACGCTGCAGCAGATCATG
CGAGGGTACCGGCTGCCGCGCCCGGCTGCCTGCCCGGCGGAGGTCTACGTGCTCATGCTG
GAGTGCTGGAGGAGCAGCCCCGAGGAACGGCCCTCCTTCGCCACGCTGCGGGAGAAGCTG
CACGCCATCCACAGATGCCACCCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRMS
Symbol Nomenclature : SRMS
SYNONYM : C20orf148; SRM; dJ697K14.1
Designation : tyrosine-protein kinase Srms
Full Nomenclature : src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
GENEID : 6725
GenBank Accession : 568338
HGNC : 11298
Vega : OTTHUMG00000032977
Target GenBank: BC148643

 

Reference Sequence Alignment

  

HsCD00356544      ATGGAGCCGTTCCTCAGGAGGCGGCTGGCCTTCCTGTCCTTCTTCTGGGACAAGATCTGG
NM_080823.3       ATGGAGCCGTTCCTCAGGAGGCGGCTGGCCTTCCTGTCCTTCTTCTGGGACAAGATCTGG
                  ************************************************************

HsCD00356544      CCGGCGGGCGGCGAGCCGGACCATGGCACCCCCGGGTCCCTGGACCCCAACACTGACCCA
NM_080823.3       CCGGCGGGCGGCGAGCCGGACCATGGCACCCCCGGGTCCCTGGACCCCAACACTGACCCA
                  ************************************************************

HsCD00356544      GTGCCCACGCTCCCCGCCGAGCCTTGCAGCCCCTTCCCTCAGCTCTTCCTTGCGCTCTAT
NM_080823.3       GTGCCCACGCTCCCCGCCGAGCCTTGCAGCCCCTTCCCTCAGCTCTTCCTTGCGCTCTAT
                  ************************************************************

HsCD00356544      GACTTCACGGCGCGGTGTGGCGGGGAGCTGAGTGTCCGCCGCGGGGACAGGCTCTGTGCC
NM_080823.3       GACTTCACGGCGCGGTGTGGCGGGGAGCTGAGTGTCCGCCGCGGGGACAGGCTCTGTGCC
                  ************************************************************

HsCD00356544      CTCGAAGAGGGGGGCGGCTACATCTTCGCACGCAGGCTTTCGGGCCAGCCCAGCGCCGGG
NM_080823.3       CTCGAAGAGGGGGGCGGCTACATCTTCGCACGCAGGCTTTCGGGCCAGCCCAGCGCCGGG
                  ************************************************************

HsCD00356544      CTCGTGCCCATCACCCACGTGGCCAAGGCTTCTCCTGAGACGCTCTCAGACCAACCCTGG
NM_080823.3       CTCGTGCCCATCACCCACGTGGCCAAGGCTTCTCCTGAGACGCTCTCAGACCAACCCTGG
                  ************************************************************

HsCD00356544      TACTTTAGCGGGGTCAGTCGGACCCAGGCACAGCAGCTGCTCCTCTCCCCACCCAACGAA
NM_080823.3       TACTTTAGCGGGGTCAGTCGGACCCAGGCACAGCAGCTGCTCCTCTCCCCACCCAACGAA
                  ************************************************************

HsCD00356544      CCAGGGGCCTTCCTCATCCGGCCCAGCGAGAGCAGCCTCGGGGGCTACTCACTGTCAGTC
NM_080823.3       CCAGGGGCCTTCCTCATCCGGCCCAGCGAGAGCAGCCTCGGGGGCTACTCACTGTCAGTC
                  ************************************************************

HsCD00356544      CGGGCCCAGGCCAAGGTCTGCCACTACCGGGTCTCCATGGCAGCTGATGGCAGCCTCTAC
NM_080823.3       CGGGCCCAGGCCAAGGTCTGCCACTACCGGGTCTCCATGGCAGCTGATGGCAGCCTCTAC
                  ************************************************************

HsCD00356544      CTGCAGAAGGGACGGCTCTTTCCCGGCCTGGAGGAGCTGCTCACCTACTACAAGGCCAAC
NM_080823.3       CTGCAGAAGGGACGGCTCTTTCCCGGCCTGGAGGAGCTGCTCACCTACTACAAGGCCAAC
                  ************************************************************

HsCD00356544      TGGAAGCTGATCCAGAACCCCCTGCTGCAGCCCTGCATGCCCCAGAAGGCCCCGAGGCAG
NM_080823.3       TGGAAGCTGATCCAGAACCCCCTGCTGCAGCCCTGCATGCCCCAGAAGGCCCCGAGGCAG
                  ************************************************************

HsCD00356544      GACGTGTGGGAGCGGCCACACTCCGAATTCGCCCTTGGGAGGAAGCTGGGTGAAGGCTAC
NM_080823.3       GACGTGTGGGAGCGGCCACACTCCGAATTCGCCCTTGGGAGGAAGCTGGGTGAAGGCTAC
                  ************************************************************

HsCD00356544      TTTGGGGAGGTGTGGGAAGGCCTGTGGCTGGGCTCCCTGCCCGTGGCGATCAAGGTCATC
NM_080823.3       TTTGGGGAGGTGTGGGAAGGCCTGTGGCTGGGCTCCCTGCCCGTGGCGATCAAGGTCATC
                  ************************************************************

HsCD00356544      AAGTCAGCCAACATGAAGCTCACTGACCTCGCCAAGGAGATCCAGACACTGAAGGGCCTG
NM_080823.3       AAGTCAGCCAACATGAAGCTCACTGACCTCGCCAAGGAGATCCAGACACTGAAGGGCCTG
                  ************************************************************

HsCD00356544      CGGCACGAGCGGCTCATCCGGCTGCACGCAGTGTGCTCGGGCGGGGAGCCTGTGTACATC
NM_080823.3       CGGCACGAGCGGCTCATCCGGCTGCACGCAGTGTGCTCGGGCGGGGAGCCTGTGTACATC
                  ************************************************************

HsCD00356544      GTCACGGAACTCATGCGCAAGGGGAACCTGCAGGCCTTCCTGGGCACCCCCGAGGGCCGG
NM_080823.3       GTCACGGAACTCATGCGCAAGGGGAACCTGCAGGCCTTCCTGGGCACCCCCGAGGGCCGG
                  ************************************************************

HsCD00356544      GCCCTGCGTCTGCCGCCACTCCTGGGCTTTGCCTGCCAGGTGGCTGAGGGCATGAGCTAC
NM_080823.3       GCCCTGCGTCTGCCGCCACTCCTGGGCTTTGCCTGCCAGGTGGCTGAGGGCATGAGCTAC
                  ************************************************************

HsCD00356544      CTGGAGGAGCAGCGCGTTGTGCACCGGGACTTGGCCGCCCGGAACGTGCTCGTGGACGAC
NM_080823.3       CTGGAGGAGCAGCGCGTTGTGCACCGGGACTTGGCCGCCCGGAACGTGCTCGTGGACGAC
                  ************************************************************

HsCD00356544      GGCCTGGCCTGCAAGGTGGCTGACTTCGGCCTGGCCCGGCTGCTCAAGGACGACATCTAC
NM_080823.3       GGCCTGGCCTGCAAGGTGGCTGACTTCGGCCTGGCCCGGCTGCTCAAGGACGACATCTAC
                  ************************************************************

HsCD00356544      TCCCCGAGCAGCAGCTCCAAGATCCCGGTCAAGTGGACAGCGCCTGAGGCGGCCAATTAT
NM_080823.3       TCCCCGAGCAGCAGCTCCAAGATCCCGGTCAAGTGGACAGCGCCTGAGGCGGCCAATTAT
                  ************************************************************

HsCD00356544      CGTGTCTTCTCCCAGAAGTCAGACGTCTGGTCCTTCGGCGTCCTGCTGCACGAGGTTTTC
NM_080823.3       CGTGTCTTCTCCCAGAAGTCAGACGTCTGGTCCTTCGGCGTCCTGCTGCACGAGGTTTTC
                  ************************************************************

HsCD00356544      ACCTATGGCCAGTGTCCCTATGAAGGGATGACCAACCACGAGACGCTGCAGCAGATCATG
NM_080823.3       ACCTATGGCCAGTGTCCCTATGAAGGGATGACCAACCACGAGACGCTGCAGCAGATCATG
                  ************************************************************

HsCD00356544      CGAGGGTACCGGCTGCCGCGCCCGGCTGCCTGCCCGGCGGAGGTCTACGTGCTCATGCTG
NM_080823.3       CGAGGGTACCGGCTGCCGCGCCCGGCTGCCTGCCCGGCGGAGGTCTACGTGCTCATGCTG
                  ************************************************************

HsCD00356544      GAGTGCTGGAGGAGCAGCCCCGAGGAACGGCCCTCCTTCGCCACGCTGCGGGAGAAGCTG
NM_080823.3       GAGTGCTGGAGGAGCAGCCCCGAGGAACGGCCCTCCTTCGCCACGCTGCGGGAGAAGCTG
                  ************************************************************

HsCD00356544      CACGCCATCCACAGATGCCACCCC---
NM_080823.3       CACGCCATCCACAGATGCCACCCCTGA
                  ************************   

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen activates B Cell Receptor (BCR) leading to generation of second messengers
Reactome : Immune System
Reactome : Signaling by the B Cell Receptor (BCR)
WikiPathway : Focal Adhesion

Clone

Cloning Information : 568338

Organism


TAX_ID : 9606
Species Specific ID: 6725

Genome

Chromosome : 20
Ensembl : ENSG00000125508

Reagents

Labome : SRMS-antibody