DNASU Plasmid Repository • 480.965.5697 | Email

UBE2L3 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  UBE2L3
Gene Name:  ubiquitin-conjugating enzyme E2L 3
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: Clone contains stop codon; generated from ORFeome entry clone (IMAGE:100000459)
Mutation/
Discrepancy :
No / No
Publications: PMID: 25023374
Title: Middle-Down Mass Spectrometry Enables Characterization of Branched Ubiquitin Chains.
Authors: Center for Personalized Diagnostics
HsCD00356713

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 

Insert sequence: 462nts         Open reading frame : 1 to 462
Reference Sequence Alignment

ATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAA
AACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTT
CCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAG
TACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGAC
GAAAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAA
ACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCG
CTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCT
GAAGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2L3
Symbol Nomenclature : UBE2L3
SYNONYM : E2-F1; L-UBC; UBCH7; UbcM4
Designation : ubiquitin carrier protein L3|ubiquitin-conjugating enzyme E2 L3|ubiquitin-conjugating enzyme E2-F1|ubiquitin-conjugating enzyme UBCH7|ubiquitin-protein ligase L3
Full Nomenclature : ubiquitin-conjugating enzyme E2L 3
GENEID : 7332
GenBank Accession : 568816
HGNC : 12488
MIM : 603721
Vega : OTTHUMG00000150823
Target GenBank: CU013490

 

Reference Sequence Alignment

  

HsCD00356713        ------------------------------------------------------------
NM_003347.3         ------------------------------------------------------------
NM_001256355.1      ATGCAGGTCGCTGCAGGGACCCGAGGCGACACGCGCCTGCAGGAGGTGGCACTCCTGCCG
                                                                                

HsCD00356713        ------------------------------------------------------------
NM_003347.3         ------------------------------------------------------------
NM_001256355.1      CAGCTCTTCGATCTGCTTGTCCTTGGCCAGCGGCGCGCTCGCCTGCTCCGACAGGTCCCG
                                                                                

HsCD00356713        ------------------------------------------------------ATGGCG
NM_003347.3         ------------------------------------------------------ATGGCG
NM_001256355.1      AGCGCGCTGGCGGGCAAAGACTTGGCACAGCTCCAGGCCGGCGCAACTCTCGCCGGGTAT
                                                                          . * . 

HsCD00356713        GCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAAAACTTC
NM_003347.3         GCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAAAACTTC
NM_001256355.1      AGGCGCGCTCACGGCCCGGAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAAAACTTC
                    .  .**.  ..    . *.*****************************************

HsCD00356713        CGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGAC
NM_003347.3         CGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGAC
NM_001256355.1      CGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGAC
                    ************************************************************

HsCD00356713        AACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCA
NM_003347.3         AACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCA
NM_001256355.1      AACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCA
                    ************************************************************

HsCD00356713        TTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAG
NM_003347.3         TTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAG
NM_001256355.1      TTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAG
                    ************************************************************

HsCD00356713        GGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGAC
NM_003347.3         GGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGAC
NM_001256355.1      GGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGAC
                    ************************************************************

HsCD00356713        CAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGG
NM_003347.3         CAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGG
NM_001256355.1      CAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCTTCGG
                    ************************************************************

HsCD00356713        GCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAAGAG
NM_003347.3         GCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAAGAG
NM_001256355.1      GCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAAGAG
                    ************************************************************

HsCD00356713        TTTACAAAGAAATATGGGGAAAAGCGACCTGTGGAC---
NM_003347.3         TTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAA
NM_001256355.1      TTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAA
                    ************************************   

Pathways

NCI : Alpha-synuclein signaling
NCI : BARD1 signaling events
Panther : Parkinson disease
Panther : Ubiquitin proteasome pathway
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Clone

Cloning Information : 568816

Organism


TAX_ID : 9606
Species Specific ID: 7332

Genome

Chromosome : 22
Map Location : 22q11.21
Ensembl : ENSG00000185651

Reagents

Labome : UbcH7-antibody