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CDC37L1 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CDC37L1
Gene Name:  cell division cycle 37-like 1
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100070508)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00357194

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1011nts         Open reading frame : 1 to 1011
Reference Sequence Alignment

ATGGAACAACCGTGGCCGCCTCCGGGACCCTGGAGCCTCCCTCGGGCCGAGGGTGAGGCT
GAGGAAGAGAGTGACTTCGACGTGTTCCCCAGTTCTCCCCGCTGCCCGCAGCTGCCAGGC
GGCGGCGCCCAGATGTATAGCCATGGAATTGAATTGGCTTGCCAAAAGCAGAAAGAGTTT
GTGAAGAGCTCTGTGGCGTGCAAATGGAATCTTGCTGAAGCTCAACAGAAACTTGGTAGC
TTAGCACTGCATAATTCTGAGTCCTTGGATCAGGAGCATGCCAAAGCACAAACAGCAGTA
TCAGAACTGAGGCAACGGGAAGAAGAGTGGCGACAGAAAGAAGAAGCTCTAGTACAAAGA
GAGAAGATGTGTCTGTGGAGCACGGATGCCATTAGCAAGGATGTTTTTAATAAGAGTTTT
ATTAATCAAGATAAAAGAAAAGACACAGAAGATGAAGATAAATCAGAATCATTTATGCAA
AAATATGAGCAAAAAATCAGACATTTTGGTATGTTGAGTCGATGGGATGATAGCCAGAGA
TTTTTGTCTGACCATCCATACCTTGTATGTGAAGAAACTGCTAAATATCTTATTTTATGG
TGTTTTCACCTGGAAGCTGAGAAGAAAGGGGCTTTAATGGAACAAATAGCACATCAAGCT
GTTGTAATGCAGTTTATTATGGAAATGGCCAAAAACTGTAATGTGGATCCAAGAGGGTGT
TTTCGTTTATTTTTCCAGAAAGCCAAAGCAGAGGAAGAAGGTTATTTTGAAGCATTCAAA
AATGAACTTGAAGCTTTCAAGTCAAGAGTAAGACTTTATTCTCAATCACAAAGTTTTCAA
CCTATGACAGTTCAGAATCATGTTCCCCATTCTGGTGTTGGATCTATAGGTTTATTAGAA
TCCTTACCACAGAATCCAGATTATCTTCAGTATTCTATCAGTACAGCTCTCTGCAGCTTA
AACTCGGTGGTACATAAAGAAGATGATGAACCCAAAATGATGGACACTGTA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDC37L1
Symbol Nomenclature : CDC37L1
SYNONYM : CDC37B; HARC
Designation : CDC37 cell division cycle 37 homolog-like 1|Hsp90-associating relative of Cdc37|cell division cycle 37 homolog-like 1|hsp90 co-chaperone Cdc37-like 1
Full Nomenclature : cell division cycle 37-like 1
GENEID : 55664
Locus Tag : RP11-6J24.5
GenBank Accession : 569454
HGNC : 17179
MIM : 610346
Target GenBank: HQ447209

 

Reference Sequence Alignment

  

HsCD00357194      ATGGAACAACCGTGGCCGCCTCCGGGACCCTGGAGCCTCCCTCGGGCCGAGGGTGAGGCT
NM_017913.2       ATGGAACAACCGTGGCCGCCTCCGGGACCCTGGAGCCTCCCTCGGGCCGAGGGTGAGGCT
                  ************************************************************

HsCD00357194      GAGGAAGAGAGTGACTTCGACGTGTTCCCCAGTTCTCCCCGCTGCCCGCAGCTGCCAGGC
NM_017913.2       GAGGAAGAGAGTGACTTCGACGTGTTCCCCAGTTCTCCCCGCTGCCCGCAGCTGCCAGGC
                  ************************************************************

HsCD00357194      GGCGGCGCCCAGATGTATAGCCATGGAATTGAATTGGCTTGCCAAAAGCAGAAAGAGTTT
NM_017913.2       GGCGGCGCCCAGATGTATAGCCATGGAATTGAATTGGCTTGCCAAAAGCAGAAAGAGTTT
                  ************************************************************

HsCD00357194      GTGAAGAGCTCTGTGGCGTGCAAATGGAATCTTGCTGAAGCTCAACAGAAACTTGGTAGC
NM_017913.2       GTGAAGAGCTCTGTGGCGTGCAAATGGAATCTTGCTGAAGCTCAACAGAAACTTGGTAGC
                  ************************************************************

HsCD00357194      TTAGCACTGCATAATTCTGAGTCCTTGGATCAGGAGCATGCCAAAGCACAAACAGCAGTA
NM_017913.2       TTAGCACTGCATAATTCTGAGTCCTTGGATCAGGAGCATGCCAAAGCACAAACAGCAGTA
                  ************************************************************

HsCD00357194      TCAGAACTGAGGCAACGGGAAGAAGAGTGGCGACAGAAAGAAGAAGCTCTAGTACAAAGA
NM_017913.2       TCAGAACTGAGGCAACGGGAAGAAGAGTGGCGACAGAAAGAAGAAGCTCTAGTACAAAGA
                  ************************************************************

HsCD00357194      GAGAAGATGTGTCTGTGGAGCACGGATGCCATTAGCAAGGATGTTTTTAATAAGAGTTTT
NM_017913.2       GAGAAGATGTGTCTGTGGAGCACGGATGCCATTAGCAAGGATGTTTTTAATAAGAGTTTT
                  ************************************************************

HsCD00357194      ATTAATCAAGATAAAAGAAAAGACACAGAAGATGAAGATAAATCAGAATCATTTATGCAA
NM_017913.2       ATTAATCAAGATAAAAGAAAAGACACAGAAGATGAAGATAAATCAGAATCATTTATGCAA
                  ************************************************************

HsCD00357194      AAATATGAGCAAAAAATCAGACATTTTGGTATGTTGAGTCGATGGGATGATAGCCAGAGA
NM_017913.2       AAATATGAGCAAAAAATCAGACATTTTGGTATGTTGAGTCGATGGGATGATAGCCAGAGA
                  ************************************************************

HsCD00357194      TTTTTGTCTGACCATCCATACCTTGTATGTGAAGAAACTGCTAAATATCTTATTTTATGG
NM_017913.2       TTTTTGTCTGACCATCCATACCTTGTATGTGAAGAAACTGCTAAATATCTTATTTTATGG
                  ************************************************************

HsCD00357194      TGTTTTCACCTGGAAGCTGAGAAGAAAGGGGCTTTAATGGAACAAATAGCACATCAAGCT
NM_017913.2       TGTTTTCACCTGGAAGCTGAGAAGAAAGGGGCTTTAATGGAACAAATAGCACATCAAGCT
                  ************************************************************

HsCD00357194      GTTGTAATGCAGTTTATTATGGAAATGGCCAAAAACTGTAATGTGGATCCAAGAGGGTGT
NM_017913.2       GTTGTAATGCAGTTTATTATGGAAATGGCCAAAAACTGTAATGTGGATCCAAGAGGGTGT
                  ************************************************************

HsCD00357194      TTTCGTTTATTTTTCCAGAAAGCCAAAGCAGAGGAAGAAGGTTATTTTGAAGCATTCAAA
NM_017913.2       TTTCGTTTATTTTTCCAGAAAGCCAAAGCAGAGGAAGAAGGTTATTTTGAAGCATTCAAA
                  ************************************************************

HsCD00357194      AATGAACTTGAAGCTTTCAAGTCAAGAGTAAGACTTTATTCTCAATCACAAAGTTTTCAA
NM_017913.2       AATGAACTTGAAGCTTTCAAGTCAAGAGTAAGACTTTATTCTCAATCACAAAGTTTTCAA
                  ************************************************************

HsCD00357194      CCTATGACAGTTCAGAATCATGTTCCCCATTCTGGTGTTGGATCTATAGGTTTATTAGAA
NM_017913.2       CCTATGACAGTTCAGAATCATGTTCCCCATTCTGGTGTTGGATCTATAGGTTTATTAGAA
                  ************************************************************

HsCD00357194      TCCTTACCACAGAATCCAGATTATCTTCAGTATTCTATCAGTACAGCTCTCTGCAGCTTA
NM_017913.2       TCCTTACCACAGAATCCAGATTATCTTCAGTATTCTATCAGTACAGCTCTCTGCAGCTTA
                  ************************************************************

HsCD00357194      AACTCGGTGGTACATAAAGAAGATGATGAACCCAAAATGATGGACACTGTA---
NM_017913.2       AACTCGGTGGTACATAAAGAAGATGATGAACCCAAAATGATGGACACTGTATAA
                  ***************************************************   

Protein

SMART domain : CDC37_M : Cdc37 Hsp90 binding domain
UniProt : Q7L3B6
HPRD : 07120

Clone

Cloning Information : 569454

Organism


TAX_ID : 9606
Species Specific ID: 55664

Genome

Chromosome : 9
Map Location : 9p24.1