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APIP (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  APIP
Gene Name:  APAF1 interacting protein
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100071545)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00357385

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 726nts         Open reading frame : 1 to 726
Reference Sequence Alignment

ATGTCTGGCTGTGATGCTTGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTATGATATAAATGAA
AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
GCTGTGATGGCCACCCTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGCA
ATTGTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : APIP
Symbol Nomenclature : APIP
SYNONYM : APIP2; CGI29; MMRP19; hAPIP
Designation : APAF1-interacting protein|MTRu-1-P dehydratase|methylthioribulose-1-phosphate dehydratase|probable methylthioribulose-1-phosphate dehydratase
Full Nomenclature : APAF1 interacting protein
GENEID : 51074
Locus Tag : CGI-29
GenBank Accession : 569664
HGNC : 17581
MIM : 612491
Vega : OTTHUMG00000166455
Target GenBank: HQ448158

 

Reference Sequence Alignment

  

HsCD00357385      ATGTCTGGCTGTGATGCTTGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
NM_015957.3       ATGTCTGGCTGTGATGCTCGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGAC
                  ****************** *****************************************

HsCD00357385      AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
NM_015957.3       AAGGAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGG
                  ************************************************************

HsCD00357385      GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
NM_015957.3       GTCACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCT
                  ************************************************************

HsCD00357385      TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTATGATATAAATGAA
NM_015957.3       TCAGGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTGTGATATAAATGAA
                  **********************************************.*************

HsCD00357385      AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
NM_015957.3       AAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTT
                  ************************************************************

HsCD00357385      TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
NM_015957.3       TTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCT
                  ************************************************************

HsCD00357385      GCTGTGATGGCCACCCTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
NM_015957.3       GCTGTGATGGCCACACTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG
                  **************.*********************************************

HsCD00357385      ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
NM_015957.3       ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTG
                  ************************************************************

HsCD00357385      GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
NM_015957.3       GTACCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCA
                  ************************************************************

HsCD00357385      ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
NM_015957.3       ATGAATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGG
                  ************************************************************

HsCD00357385      GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
NM_015957.3       GGGGAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATT
                  ************************************************************

HsCD00357385      GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGCA
NM_015957.3       GCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGCTCCCAGTTGGAGAAAATGGA
                  ********************************************************** *

HsCD00357385      ATTGTC---
NM_015957.3       ATTGTCTAA
                  ******   

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Clone

Cloning Information : 569664

Organism


TAX_ID : 9606
Species Specific ID: 51074

Genome

Chromosome : 11
Map Location : 11p13
Ensembl : ENSG00000149089

Reagents

Labome : APIP-antibody