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LGALS14 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS14
Gene Name:  lectin, galactoside-binding, soluble, 14
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100071049)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00358007

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 417nts         Open reading frame : 1 to 417
Reference Sequence Alignment

ATGTCATCACTACCCGTACCATACACACTGCCTGTTTCCTTGCCTGTTGGTTCGTGCGTG
ATAATCACAGGGACACCGATCCTCACTTTTGTCAAGGACCCACAGCTGGAGGTGAATTTC
TACACTGGGATGGATGAGGACTCAGATATTGCTTTCCAATTCCGACTGCACTTTGGTCAT
CCTGCAATCATGAACAGTCGTGTGTTTGGCATATGGAGATATGAGGAGAAATGCTACTAT
TTACCCTTTGAAGATGGCAAACCATTTGAGCTGTGCATCTATGTGCGTCACAAGGAATAC
AAGGTAATGGTAAATGGCCAACGCATTTACAACTTTGCCCATCGATTCCCGCCAGCATCT
GTGAAGATGCTGCAAGTCTTGAGAGATATCTCCCTGACCAGAGTGCTTATCAGCGAT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS14
Symbol Nomenclature : LGALS14
SYNONYM : CLC2; PPL13
Designation : Charcot-Leyden crystal protein 2|gal-14|galectin-14|placental protein 13-like|placental protein 13-like protein
Full Nomenclature : lectin, galactoside-binding, soluble, 14
GENEID : 56891
GenBank Accession : 570394
HGNC : 30054
MIM : 607260
Vega : OTTHUMG00000183143
Target GenBank: HQ447718

 

Reference Sequence Alignment

  

HsCD00358007      ------------------------------------------------------------
NM_020129.2       ------------------------------------------------------------
NM_203471.1       ATGTCCCTGACCCACAGGCTTCATTTGTGCAAGTACTGGGGCTGTGCTGTCAGTAATGTG
                                                                              

HsCD00358007      ---------------------------ATGTCATCACTACCCGTACCATACACACTGCCT
NM_020129.2       ---------------------------ATGTCATCACTACCCGTACCATACACACTGCCT
NM_203471.1       TGCCGCTTCTGGGAAGGACGTCCATTGCCCTTGATGATTGTGGTACCATACACACTGCCT
                                             .  * .: ..*:   ******************

HsCD00358007      GTTTCCTTGCCTGTTGGTTCGTGCGTGATAATCACAGGGACACCGATCCTCACTTTTGTC
NM_020129.2       GTTTCCTTGCCTGTTGGTTCGTGCGTGATAATCACAGGGACACCGATCCTCACTTTTGTC
NM_203471.1       GTTTCCTTGCCTGTTGGTTCGTGCGTGATAATCACAGGGACACCGATCCTCACTTTTGTC
                  ************************************************************

HsCD00358007      AAGGACCCACAGCTGGAGGTGAATTTCTACACTGGGATGGATGAGGACTCAGATATTGCT
NM_020129.2       AAGGACCCACAGCTGGAGGTGAATTTCTACACTGGGATGGATGAGGACTCAGATATTGCT
NM_203471.1       AAGGACCCACAGCTGGAGGTGAATTTCTACACTGGGATGGATGAGGACTCAGATATTGCT
                  ************************************************************

HsCD00358007      TTCCAATTCCGACTGCACTTTGGTCATCCTGCAATCATGAACAGTCGTGTGTTTGGCATA
NM_020129.2       TTCCAATTCCGACTGCACTTTGGTCATCCTGCAATCATGAACAGTTGTGTGTTTGGCATA
NM_203471.1       TTCCAATTCCGACTGCACTTTGGTCATCCTGCAATCATGAACAGTTGTGTGTTTGGCATA
                  ********************************************* **************

HsCD00358007      TGGAGATATGAGGAGAAATGCTACTATTTACCCTTTGAAGATGGCAAACCATTTGAGCTG
NM_020129.2       TGGAGATATGAGGAGAAATGCTACTATTTACCCTTTGAAGATGGCAAACCATTTGAGCTG
NM_203471.1       TGGAGATATGAGGAGAAATGCTACTATTTACCCTTTGAAGATGGCAAACCATTTGAGCTG
                  ************************************************************

HsCD00358007      TGCATCTATGTGCGTCACAAGGAATACAAGGTAATGGTAAATGGCCAACGCATTTACAAC
NM_020129.2       TGCATCTATGTGCGTCACAAGGAATACAAGGTAATGGTAAATGGCCAACGCATTTACAAC
NM_203471.1       TGCATCTATGTGCGTCACAAGGAATACAAGGTAATGGTAAATGGCCAACGCATTTACAAC
                  ************************************************************

HsCD00358007      TTTGCCCATCGATTCCCGCCAGCATCTGTGAAGATGCTGCAAGTCTTGAGAGATATCTCC
NM_020129.2       TTTGCCCATCGATTCCCGCCAGCATCTGTGAAGATGCTGCAAGTCTTCAGAGATATCTCC
NM_203471.1       TTTGCCCATCGATTCCCGCCAGCATCTGTGAAGATGCTGCAAGTCTTCAGAGATATCTCC
                  *********************************************** ************

HsCD00358007      CTGACCAGAGTGCTTATCAGCGAT---
NM_020129.2       CTGACCAGAGTGCTTATCAGCGATTGA
NM_203471.1       CTGACCAGAGTGCTTATCAGCGATTGA
                  ************************   

Protein

SMART domain : Gal-bind_lectin : Galactoside-binding lectin
SMART domain : GLECT : Galectin
UniProt : B2R530
UniProt : A8MUM7
UniProt : Q8TCE9
UniProt : A8MPV8
HPRD : 06268

Clone

Cloning Information : 570394

Organism


TAX_ID : 9606
Species Specific ID: 56891

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000006659

Reagents

Labome : LGALS14-antibody