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GLT6D1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GLT6D1
Gene Name:  glycosyltransferase 6 domain containing 1
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358263

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 735nts         Open reading frame : 1 to 735
Reference Sequence Alignment

CTCTCAGACTGGTTTCATCCTAGAAAACGCCCTGATGTTATAACGAAAACAGACTGGCTC
GCTCCTGTCCTATGGGAAGGGACTTTCGACAGGCGGGTCCTGGAAAAACATTACAGAAGG
CGGAATATCACTGTGGGCCTGGCCGTCTTTGCTACTGGCAGGTTTGCAGAGGAGTACCTG
AGGCCGTTCCTACACTCCGCAAATAAGCACTTCATGACAGGCTACCGAGTGATCTTCTAC
ATCATGGTGGACGCCTTCTTCAAGCTGCCTGACATAGAGCCCAGTCCTCTTCGAACGTTC
AAAGCATTTAAAGTGGGCACCGAGAGGTGGTGGCTCGATGGCCCCCTGGTGCATGTGAAG
AGCCTGGGTGAACACATCGCCAGTCACATCCAGGACGAGGTGGACTTCCTCTTCAGCATG
GCTGCCAACCAGGTCTTCCAGAATGAGTTCGGGGTGGAGACCCTGGGCCCGTTGGTGGCC
CAGCTCCACGCCTGGTGGTATTTCAGAAACACCAAGAACTTCCCTTATGAGAGGAGGCCG
ACCTCAGCAGCTTGCATCCCGTTTGGACAGGGAGATTTCTATTATGGCAACTTGATGGTT
GGTGGCACACCCCATAATATTTTAGACTTCATCAAAGAATATCTGAACGGAGTTATTCAT
GACATCAAAAATGGACTCAATAGCACTTATGAAAAGCACCTTAACAAATATTTTTACCTC
AATAAACCCACCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,96

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GLT6D1
Symbol Nomenclature : GLT6D1
SYNONYM : GLTDC1; GT6M7
Designation : galactosyltransferase family 6 domain containing 1|galactosyltransferase family 6 domain-containing 1|glycosyltransferase 6 domain-containing protein 1
Full Nomenclature : glycosyltransferase 6 domain containing 1
GENEID : 360203
Locus Tag : RP11-98L5.1
GenBank Accession : BC148647
HGNC : 23671
MIM : 613699
Vega : OTTHUMG00000020911
Target GenBank: BC148647

 

Reference Sequence Alignment

  

HsCD00358263      ------------------------------------------------------------
NM_182974.2       ATGAATTCTAAAAGAATGCTGTTATTGGTTTTATTTGCTTTTTCACTGATGTTGGTTGAG
                                                                              

HsCD00358263      ------------------------------------CTCTCAGACTGGTTTCATCCTAGA
NM_182974.2       CGTTATTTCAGGAATCACCAAGTAGAAGAACTTCGGCTCTCAGACTGGTTTCATCCTAGA
                                                      ************************

HsCD00358263      AAACGCCCTGATGTTATAACGAAAACAGACTGGCTCGCTCCTGTCCTATGGGAAGGGACT
NM_182974.2       AAACGCCCTGATGTTATAACGAAAACAGACTGGCTCGCTCCTGTCCTATGGGAAGGGACT
                  ************************************************************

HsCD00358263      TTCGACAGGCGGGTCCTGGAAAAACATTACAGAAGGCGGAATATCACTGTGGGCCTGGCC
NM_182974.2       TTCGACAGGCGGGTCCTGGAAAAACATTACAGAAGGCGGAATATCACTGTGGGCCTGGCC
                  ************************************************************

HsCD00358263      GTCTTTGCTACTGGCAGGTTTGCAGAGGAGTACCTGAGGCCGTTCCTACACTCCGCAAAT
NM_182974.2       GTCTTTGCTACTGGCAGGTTTGCAGAGGAGTACCTGAGGCCGTTCCTACACTCCGCAAAT
                  ************************************************************

HsCD00358263      AAGCACTTCATGACAGGCTACCGAGTGATCTTCTACATCATGGTGGACGCCTTCTTCAAG
NM_182974.2       AAGCACTTCATGACAGGCTACCGAGTGATCTTCTACATCATGGTGGACGCCTTCTTCAAG
                  ************************************************************

HsCD00358263      CTGCCTGACATAGAGCCCAGTCCTCTTCGAACGTTCAAAGCATTTAAAGTGGGCACCGAG
NM_182974.2       CTGCCTGACATAGAGCCCAGTCCTCTTCGAACGTTCAAAGCATTTAAAGTGGGCACCGAG
                  ************************************************************

HsCD00358263      AGGTGGTGGCTCGATGGCCCCCTGGTGCATGTGAAGAGCCTGGGTGAACACATCGCCAGT
NM_182974.2       AGGTGGTGGCTCGATGGCCCCCTGGTGCATGTGAAGAGCCTGGGTGAACACATCGCCAGT
                  ************************************************************

HsCD00358263      CACATCCAGGACGAGGTGGACTTCCTCTTCAGCATGGCTGCCAACCAGGTCTTCCAGAAT
NM_182974.2       CACATCCAGGACGAGGTGGACTTCCTCTTCAGCATGGCTGCCAACCAGGTCTTCCAGAAT
                  ************************************************************

HsCD00358263      GAGTTCGGGGTGGAGACCCTGGGCCCGTTGGTGGCCCAGCTCCACGCCTGGTGGTATTTC
NM_182974.2       GAGTTCGGGGTGGAGACCCTGGGCCCGTTGGTGGCCCAGCTCCACGCCTGGTGGTATTTC
                  ************************************************************

HsCD00358263      AGAAACACCAAGAACTTCCCTTATGAGAGGAGGCCGACCTCAGCAGCTTGCATCCCGTTT
NM_182974.2       AGAAACACCAAGAACTTCCCTTATGAGAGGAGGCCGACCTCAGCAGCTTGCATCCCGTTT
                  ************************************************************

HsCD00358263      GGACAGGGAGATTTCTATTATGGCAACTTGATGGTTGGTGGCACACCCCATAATATTTTA
NM_182974.2       GGACAGGGAGATTTCTATTATGGCAACTTGATGGTTGGTGGCACACCCCATAATATTTTA
                  ************************************************************

HsCD00358263      GACTTCATCAAAGAATATCTGAACGGAGTTATTCATGACATCAAAAATGGACTCAATAGC
NM_182974.2       GACTTCATCAAAGAATATCTGAACGGAGTTATTCATGACATCAAAAATGGACTCAATAGC
                  ************************************************************

HsCD00358263      ACTTATGAAAAGCACCTTAACAAATATTTTTACCTCAATAAACCCACCTAG
NM_182974.2       ACTTATGAAAAGCACCTTAACAAATATTTTTACCTCAATAAACCCACCTAG
                  ***************************************************

Protein

UniProt : Q7Z4J2
UniProt : Q5T6T3
HPRD : 13584

Clone

Cloning Information : 560362

Organism


TAX_ID : 9606
Species Specific ID: 360203

Genome

Chromosome : 9
Map Location : 9q34.3
Ensembl : ENSG00000204007

Reagents

Labome : GLT6D1-antibody