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UGT2B17 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UGT2B17
Gene Name:  UDP glucuronosyltransferase 2 family, polypeptide B17
Sequence             
Original Clone ID: None
Keyword: partial cds (N and C terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358264

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1413nts         Open reading frame : 1 to 1413
Reference Sequence Alignment

GGAAAGGTGCTGGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAGACAATCCTG
GAAGAGCTTGTTCAGAGGGGTCATGAGGTGATTGTGTTGACATCTTCGGCTTCTATTCTT
GTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCTTTAACTAAA
AATGATTTGGAAGATTTTTTTATGAAAATGTTCGATAGATGGACATATAGTATTTCAAAA
AATACATTTTGGTCATATTTTTCACAACTACAAGAATTGTGTTGGGAATATTCTGACTAT
AATATAAAGCTCTGTGAAGATGCAGTTTTGAACAAGAAACTTATGAGAAAACTACAAGAG
TCAAAATTTGATGTCCTTCTGGCAGATGCCGTTAATCCCTGTGGTGAGCTGCTGGCTGAA
CTACTTAACATACCCTTTCTGTACAGTCTCCGCTTCTCTGTTGGCTACACAGTTGAGAAG
AATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCAGAATTAAGT
GATCAAATGATTTTCATGGAGAGGATAAAAAATATGATATATATGCTTTATTTTGACTTT
TGGTTTCAAGCATATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTTCTAGGAAGA
CCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGAACCTATTGG
GATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGACTTCACTGT
AAACCAGCCAAACCCTTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCTGGAGAAAAT
GGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAAAGTGCCAAC
ATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTTGATGGCAAG
AAGCCAAATACTTTAGGTTCCAATACTCGACTGTATAAGTGGTTACCCCAGAATGACCTT
CTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGCATCTATGAG
GCGATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAACATGATAAC
ATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACCATGTCAAGT
AGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTATCTATAAAGAGAATATC
ATGAAATTATCAAGAATTCATCATGATCAACCGGTGAAGCCCCTGGATCGAGCAGTCTTC
TGGATTGAGTTTGTCATGCGCCATAAAGGAGCCAAGCACCTTCGGGTCGCAGCCCACAAC
CTCACCTGGATCCAGTACCACTCTTTGGATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,69; del@1480,111

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UGT2B17
Symbol Nomenclature : UGT2B17
SYNONYM : BMND12; UDPGT2B17
Designation : C19-steroid-specific UDP-glucuronosyltransferase|C19-steroid-specific UDPGT|UDP glycosyltransferase 2 family, member B17|UDP-glucuronosyltransferase 2B17|UDP-glucuronyltransferase, family 2, beta-17
Full Nomenclature : UDP glucuronosyltransferase 2 family, polypeptide B17
GENEID : 7367
GenBank Accession : BC141517
HGNC : 12547
MIM : 601903
Vega : OTTHUMG00000129305
Target GenBank: BC141517

 

Reference Sequence Alignment

  

HsCD00358264      ------------------------------------------------------------
NM_001076.3       ATGTCTCTGAAATGGACGTCAGTCTTTCTGCTGATACAGCTCAGTTGTTACTTTAGCTCT
NM_001077.3       ATGTCTCTGAAATGGATGTCAGTCTTTCTGCTGATGCAGCTCAGTTGTTACTTTAGCTCT
                                                                              

HsCD00358264      ---------GGAAAGGTGCTGGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAG
NM_001076.3       GGAAGCTGTGGAAAGGTGCTAGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAG
NM_001077.3       GGGAGTTGTGGAAAGGTGCTGGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAG
                           ***********.***************************************

HsCD00358264      ACAATCCTGGAAGAGCTTGTTCAGAGGGGTCATGAGGTGATTGTGTTGACATCTTCGGCT
NM_001076.3       ACAATCCTGGAAGAGCTTGTTCAGAGGGGTCATGAGGTGACTGTGTTGACATCTTCGGCT
NM_001077.3       ACAATCCTGGAAGAGCTTGTTCAGAGGGGTCATGAGGTGATTGTGTTGACATCTTCGGCT
                  **************************************** *******************

HsCD00358264      TCTATTCTTGTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCT
NM_001076.3       TCTACTCTTGTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCT
NM_001077.3       TCTATTCTTGTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCT
                  **** *******************************************************

HsCD00358264      TTAACTAAAAATGATTTGGAAGATTTTTTTATGAAAATGTTCGATAGATGGACATATAGT
NM_001076.3       TTAACTAAAAATTATTTGGAAGATTCTCTTCTGAAAATTCTCGATAGATGGATATATGGT
NM_001077.3       TTAACTAAAAATGATTTGGAAGATTTTTTTATGAAAATGTTCGATAGATGGACATATAGT
                  ************ ************ * **.*******  ************ ****.**

HsCD00358264      ATTTCAAAAAATACATTTTGGTCATATTTTTCACAACTACAAGAATTGTGTTGGGAATAT
NM_001076.3       GTTTCAAAAAATACATTTTGGTCATATTTTTCACAATTACAAGAATTGTGTTGGGAATAT
NM_001077.3       ATTTCAAAAAATACATTTTGGTCATATTTTTCACAACTACAAGAATTGTGTTGGGAATAT
                  .*********************************** ***********************

HsCD00358264      TCTGACTATAATATAAAGCTCTGTGAAGATGCAGTTTTGAACAAGAAACTTATGAGAAAA
NM_001076.3       TATGACTACAGTAACAAGCTCTGTAAAGATGCAGTTTTGAATAAGAAACTTATGATGAAA
NM_001077.3       TCTGACTATAATATAAAGCTCTGTGAAGATGCAGTTTTGAACAAGAAACTTATGAGAAAA
                  *.****** *.**:.*********.**************** ************* .***

HsCD00358264      CTACAAGAGTCAAAATTTGATGTCCTTCTGGCAGATGCCGTTAATCCCTGTGGTGAGCTG
NM_001076.3       CTACAAGAGTCAAAGTTTGATGTCATTCTGGCAGATGCCCTTAATCCCTGTGGTGAGCTA
NM_001077.3       CTACAAGAGTCAAAATTTGATGTCCTTCTGGCAGATGCCGTTAATCCCTGTGGTGAGCTG
                  **************.*********.************** *******************.

HsCD00358264      CTGGCTGAACTACTTAACATACCCTTTCTGTACAGTCTCCGCTTCTCTGTTGGCTACACA
NM_001076.3       CTGGCTGAACTATTTAACATACCCTTTCTGTACAGTCTTCGATTCTCTGTTGGCTACACA
NM_001077.3       CTGGCTGAGCTACTTAACATACCCTTTCTGTACAGTCTCCGCTTCTCTGTTGGCTACACA
                  ********.*** ************************* **.******************

HsCD00358264      GTTGAGAAGAATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCA
NM_001076.3       TTTGAGAAGAATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCA
NM_001077.3       GTTGAGAAGAATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCA
                   ***********************************************************

HsCD00358264      GAATTAAGTGATCAAATGATTTTCATGGAGAGGATAAAAAATATGATATATATGCTTTAT
NM_001076.3       GAATTAAGTGATCAAATGATTTTCATGGAGAGGATAAAAAATATGATACATATGCTTTAT
NM_001077.3       GAATTAAGTGATCAAATGATTTTCATGGAGAGGATAAAAAATATGATATATATGCTTTAT
                  ************************************************ ***********

HsCD00358264      TTTGACTTTTGGTTTCAAGCATATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTT
NM_001076.3       TTTGACTTTTGGTTTCAAATTTATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTT
NM_001077.3       TTTGACTTTTGGTTTCAAGCATATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTT
                  ******************. :***************************************

HsCD00358264      CTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGA
NM_001076.3       CTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGA
NM_001077.3       CTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGA
                  ************************************************************

HsCD00358264      ACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
NM_001076.3       ACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
NM_001077.3       ACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
                  ************************************************************

HsCD00358264      CTTCACTGTAAACCAGCCAAACCCTTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCT
NM_001076.3       CTTCACTGTAAACCAGCCAAACCCCTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCT
NM_001077.3       CTTCACTGTAAACCAGCCAAACCCTTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCT
                  ************************ ***********************************

HsCD00358264      GGAGAAAATGGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAA
NM_001076.3       GGAGAAAATGGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAA
NM_001077.3       GGAGAAAATGGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAA
                  ************************************************************

HsCD00358264      AGTGCCAACATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTT
NM_001076.3       AGTGCCAACATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTT
NM_001077.3       AGTGCCAACATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTT
                  ************************************************************

HsCD00358264      GATGGCAAGAAGCCAAATACTTTAGGTTCCAATACTCGACTGTATAAGTGGTTACCCCAG
NM_001076.3       GATGGCAAGAAGCCAAATACTTTAGGTTCCAATACTCGACTGTACAAGTGGTTACCCCAG
NM_001077.3       GATGGCAAGAAGCCAAATACTTTAGGTTCCAATACTCGACTGTATAAGTGGTTACCCCAG
                  ******************************************** ***************

HsCD00358264      AATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGC
NM_001076.3       AATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGC
NM_001077.3       AATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGC
                  ************************************************************

HsCD00358264      ATCTATGAGGCGATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAA
NM_001076.3       ATCTATGAGGCGATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAA
NM_001077.3       ATCTATGAGGCAATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAA
                  ***********.************************************************

HsCD00358264      CATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
NM_001076.3       CATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
NM_001077.3       CATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
                  ************************************************************

HsCD00358264      ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTATCTATAAA
NM_001076.3       ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTGTCTATAAA
NM_001077.3       ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTATCTATAAA
                  ***************************************************.********

HsCD00358264      GAGAATATCATGAAATTATCAAGAATTCATCATGATCAACCGGTGAAGCCCCTGGATCGA
NM_001076.3       GAGAATGTCATGAAATTATCAAGAATTCATCATGACCAACCAATGAAGCCCCTGGATCGA
NM_001077.3       GAGAATATCATGAAATTATCAAGAATTCATCATGATCAACCGGTGAAGCCCCTGGATCGA
                  ******.**************************** *****..*****************

HsCD00358264      GCAGTCTTCTGGATTGAGTTTGTCATGCGCCATAAAGGAGCCAAGCACCTTCGGGTCGCA
NM_001076.3       GCAGTCTTCTGGATTGAGTTTGTCATGCGCCACAAAGGAGCCAAGCACCTTCGAGTCGCA
NM_001077.3       GCAGTCTTCTGGATTGAGTTTGTCATGCGCCATAAAGGAGCCAAGCACCTTCGGGTCGCA
                  ******************************** ********************.******

HsCD00358264      GCCCACAACCTCACCTGGATCCAGTACCACTCTTTGGATTAG------------------
NM_001076.3       GCTCACAACCTCACCTGGATCCAGTACCACTCTTTGGATGTGATAGCATTCCTGCTGGCC
NM_001077.3       GCCCACAACCTCACCTGGATCCAGTACCACTCTTTGGATGTGATAGCATTCCTGCTGGCC
                  ** ************************************ :*                  

HsCD00358264      ------------------------------------------------------------
NM_001076.3       TGCGTGGCAACTGTGATATTTATCATCACAAAATTTTGCCTGTTTTGTTTCCGAAAGCTT
NM_001077.3       TGCGTGGCAACTATGATATTTATGATCACAAAATGTTGCCTGTTTTGTTTCCGAAAGCTT
                                                                              

HsCD00358264      ---------------------------------
NM_001076.3       GCCAAAAAAGGAAAGAAGAAGAAAAGAGATTAG
NM_001077.3       GCCAAAACAGGAAAGAAGAAGAAAAGGGATTAG
                                                   

Pathways

Reactome : Biological oxidations
Reactome : Glucuronidation
Reactome : Metabolism
Reactome : Phase II conjugation
WikiPathway : Glucuronidation
WikiPathway : metapathway biotransformation

Protein

UniProt : O75795
HPRD : 03545

Clone

Cloning Information : 560363

Organism


TAX_ID : 9606
Species Specific ID: 7367

Genome

Chromosome : 4
Map Location : 4q13
Ensembl : ENSG00000197888

Reagents

Labome : UGT2B17-antibody