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UGT2B15 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UGT2B15
Gene Name:  UDP glucuronosyltransferase 2 family, polypeptide B15
Sequence             
Original Clone ID: None
Keyword: partial cds (N and C terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358265

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1413nts         Open reading frame : 1 to 1413
Reference Sequence Alignment

GGAAAGGTGCTAGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAGACAATCCTG
GAAGAGCTTGTTCAGAGGGGTCATGAGGTGACTGTGTTGACATCTTCGGCTTCTACTCTT
GTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCTTTAACTAAA
AATTATTTGGAAGATTCTCTTCTGAAAATTCTCGATAGATGGATATATGGTGTTTCAAAA
AATACATTTTGGTCATATTTTTCACAATTACAAGAATTGTGTTGGGAATATTATGACTAC
AGTAACAAGCTCTGTAAAGATGCAGTTTTGAATAAGAAACTTATGATGAAACTACAAGAG
TCAAAGTTTGATGTCATTCTGGCAGATGCCCTTAATCCCTGTGGTGAGCTACTGGCTGAA
CTATTTAACATACCCTTTCTGTACAGTCTTCGATTCTCTGTTGGCTACACATTTGAGAAG
AATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCAGAATTAAGT
GATCAAATGATTTTCATGGAGAGGATAAAAAATATGATACATATGCTTTATTTTGACTTT
TGGTTTCAAATTTATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTTCTAGGAAGA
CCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGAACCTATTGG
GATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGACTTCACTGT
AAACCAGCCAAACCCCTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCTGGAGAAAAT
GGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAAAGTGCCAAC
ATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTTGATGGCAAG
AAGCCAAATACTTTAGGTTCCAATACTCGACTGTACAAGTGGTTACCCCAGAATGACCTT
CTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGCATCTATGAG
GCGATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAACATGATAAC
ATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACCATGTCAAGT
AGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTGTCTATAAAGAGAATGTC
ATGAAATTATCAAGAATTCATCATGACCAACCAATGAAGCCCCTGGATCGAGCAGTCTTC
TGGATTGAGTTTGTCATGCGCCACAAAGGAGCCAAGCACCTTCGAGTCGCAGCTCACAAC
CTCACCTGGATCCAGTACCACTCTTTGGATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,69; del@1480,114

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UGT2B15
Symbol Nomenclature : UGT2B15
SYNONYM : HLUG4; UDPGT 2B8; UDPGT2B15; UDPGTH3; UGT2B8
Designation : UDP glycosyltransferase 2B15|UDP-glucuronosyltransferase 2B15|UDP-glucuronosyltransferase 2B8|UDP-glucuronosyltransferase UGT2B15|UDP-glucuronyltransferase, family 2, beta-15|UDPGT 2B15|UDPGTh-3
Full Nomenclature : UDP glucuronosyltransferase 2 family, polypeptide B15
GENEID : 7366
GenBank Accession : BC146570
HGNC : 12546
MIM : 600069
Vega : OTTHUMG00000161507
Target GenBank: BC146570

 

Reference Sequence Alignment

  

HsCD00358265      ------------------------------------------------------------
NM_001076.3       ATGTCTCTGAAATGGACGTCAGTCTTTCTGCTGATACAGCTCAGTTGTTACTTTAGCTCT
NM_001077.3       ATGTCTCTGAAATGGATGTCAGTCTTTCTGCTGATGCAGCTCAGTTGTTACTTTAGCTCT
                                                                              

HsCD00358265      ---------GGAAAGGTGCTAGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAG
NM_001076.3       GGAAGCTGTGGAAAGGTGCTAGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAG
NM_001077.3       GGGAGTTGTGGAAAGGTGCTGGTGTGGCCCACAGAATACAGCCATTGGATAAATATGAAG
                           ***********.***************************************

HsCD00358265      ACAATCCTGGAAGAGCTTGTTCAGAGGGGTCATGAGGTGACTGTGTTGACATCTTCGGCT
NM_001076.3       ACAATCCTGGAAGAGCTTGTTCAGAGGGGTCATGAGGTGACTGTGTTGACATCTTCGGCT
NM_001077.3       ACAATCCTGGAAGAGCTTGTTCAGAGGGGTCATGAGGTGATTGTGTTGACATCTTCGGCT
                  **************************************** *******************

HsCD00358265      TCTACTCTTGTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCT
NM_001076.3       TCTACTCTTGTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCT
NM_001077.3       TCTATTCTTGTCAATGCCAGTAAATCATCTGCTATTAAATTAGAAGTTTATCCTACATCT
                  **** *******************************************************

HsCD00358265      TTAACTAAAAATTATTTGGAAGATTCTCTTCTGAAAATTCTCGATAGATGGATATATGGT
NM_001076.3       TTAACTAAAAATTATTTGGAAGATTCTCTTCTGAAAATTCTCGATAGATGGATATATGGT
NM_001077.3       TTAACTAAAAATGATTTGGAAGATTTTTTTATGAAAATGTTCGATAGATGGACATATAGT
                  ************ ************ * **.*******  ************ ****.**

HsCD00358265      GTTTCAAAAAATACATTTTGGTCATATTTTTCACAATTACAAGAATTGTGTTGGGAATAT
NM_001076.3       GTTTCAAAAAATACATTTTGGTCATATTTTTCACAATTACAAGAATTGTGTTGGGAATAT
NM_001077.3       ATTTCAAAAAATACATTTTGGTCATATTTTTCACAACTACAAGAATTGTGTTGGGAATAT
                  .*********************************** ***********************

HsCD00358265      TATGACTACAGTAACAAGCTCTGTAAAGATGCAGTTTTGAATAAGAAACTTATGATGAAA
NM_001076.3       TATGACTACAGTAACAAGCTCTGTAAAGATGCAGTTTTGAATAAGAAACTTATGATGAAA
NM_001077.3       TCTGACTATAATATAAAGCTCTGTGAAGATGCAGTTTTGAACAAGAAACTTATGAGAAAA
                  *.****** *.**:.*********.**************** ************* .***

HsCD00358265      CTACAAGAGTCAAAGTTTGATGTCATTCTGGCAGATGCCCTTAATCCCTGTGGTGAGCTA
NM_001076.3       CTACAAGAGTCAAAGTTTGATGTCATTCTGGCAGATGCCCTTAATCCCTGTGGTGAGCTA
NM_001077.3       CTACAAGAGTCAAAATTTGATGTCCTTCTGGCAGATGCCGTTAATCCCTGTGGTGAGCTG
                  **************.*********.************** *******************.

HsCD00358265      CTGGCTGAACTATTTAACATACCCTTTCTGTACAGTCTTCGATTCTCTGTTGGCTACACA
NM_001076.3       CTGGCTGAACTATTTAACATACCCTTTCTGTACAGTCTTCGATTCTCTGTTGGCTACACA
NM_001077.3       CTGGCTGAGCTACTTAACATACCCTTTCTGTACAGTCTCCGCTTCTCTGTTGGCTACACA
                  ********.*** ************************* **.******************

HsCD00358265      TTTGAGAAGAATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCA
NM_001076.3       TTTGAGAAGAATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCA
NM_001077.3       GTTGAGAAGAATGGTGGAGGATTTCTGTTCCCTCCTTCCTATGTACCTGTTGTTATGTCA
                   ***********************************************************

HsCD00358265      GAATTAAGTGATCAAATGATTTTCATGGAGAGGATAAAAAATATGATACATATGCTTTAT
NM_001076.3       GAATTAAGTGATCAAATGATTTTCATGGAGAGGATAAAAAATATGATACATATGCTTTAT
NM_001077.3       GAATTAAGTGATCAAATGATTTTCATGGAGAGGATAAAAAATATGATATATATGCTTTAT
                  ************************************************ ***********

HsCD00358265      TTTGACTTTTGGTTTCAAATTTATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTT
NM_001076.3       TTTGACTTTTGGTTTCAAATTTATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTT
NM_001077.3       TTTGACTTTTGGTTTCAAGCATATGATCTGAAGAAGTGGGACCAGTTTTATAGTGAAGTT
                  ******************. :***************************************

HsCD00358265      CTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGA
NM_001076.3       CTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGA
NM_001077.3       CTAGGAAGACCCACTACATTATTTGAGACAATGGGGAAAGCTGAAATGTGGCTCATTCGA
                  ************************************************************

HsCD00358265      ACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
NM_001076.3       ACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
NM_001077.3       ACCTATTGGGATTTTGAATTTCCTCGCCCATTCTTACCAAATGTTGATTTTGTTGGAGGA
                  ************************************************************

HsCD00358265      CTTCACTGTAAACCAGCCAAACCCCTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCT
NM_001076.3       CTTCACTGTAAACCAGCCAAACCCCTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCT
NM_001077.3       CTTCACTGTAAACCAGCCAAACCCTTGCCTAAGGAAATGGAAGAGTTTGTGCAGAGCTCT
                  ************************ ***********************************

HsCD00358265      GGAGAAAATGGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAA
NM_001076.3       GGAGAAAATGGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAA
NM_001077.3       GGAGAAAATGGTATTGTGGTGTTTTCTCTGGGGTCGATGATCAGTAACATGTCAGAAGAA
                  ************************************************************

HsCD00358265      AGTGCCAACATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTT
NM_001076.3       AGTGCCAACATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTT
NM_001077.3       AGTGCCAACATGATTGCATCAGCCCTTGCCCAGATCCCACAAAAGGTTCTATGGAGATTT
                  ************************************************************

HsCD00358265      GATGGCAAGAAGCCAAATACTTTAGGTTCCAATACTCGACTGTACAAGTGGTTACCCCAG
NM_001076.3       GATGGCAAGAAGCCAAATACTTTAGGTTCCAATACTCGACTGTACAAGTGGTTACCCCAG
NM_001077.3       GATGGCAAGAAGCCAAATACTTTAGGTTCCAATACTCGACTGTATAAGTGGTTACCCCAG
                  ******************************************** ***************

HsCD00358265      AATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGC
NM_001076.3       AATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGC
NM_001077.3       AATGACCTTCTTGGTCATCCCAAAACCAAAGCTTTTATAACTCATGGTGGAACCAATGGC
                  ************************************************************

HsCD00358265      ATCTATGAGGCGATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAA
NM_001076.3       ATCTATGAGGCGATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAA
NM_001077.3       ATCTATGAGGCAATCTACCATGGGATCCCTATGGTGGGCATTCCCTTGTTTGCGGATCAA
                  ***********.************************************************

HsCD00358265      CATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
NM_001076.3       CATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
NM_001077.3       CATGATAACATTGCTCACATGAAAGCCAAGGGAGCAGCCCTCAGTGTGGACATCAGGACC
                  ************************************************************

HsCD00358265      ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTGTCTATAAA
NM_001076.3       ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTGTCTATAAA
NM_001077.3       ATGTCAAGTAGAGATTTGCTCAATGCATTGAAGTCAGTCATTAATGACCCTATCTATAAA
                  ***************************************************.********

HsCD00358265      GAGAATGTCATGAAATTATCAAGAATTCATCATGACCAACCAATGAAGCCCCTGGATCGA
NM_001076.3       GAGAATGTCATGAAATTATCAAGAATTCATCATGACCAACCAATGAAGCCCCTGGATCGA
NM_001077.3       GAGAATATCATGAAATTATCAAGAATTCATCATGATCAACCGGTGAAGCCCCTGGATCGA
                  ******.**************************** *****..*****************

HsCD00358265      GCAGTCTTCTGGATTGAGTTTGTCATGCGCCACAAAGGAGCCAAGCACCTTCGAGTCGCA
NM_001076.3       GCAGTCTTCTGGATTGAGTTTGTCATGCGCCACAAAGGAGCCAAGCACCTTCGAGTCGCA
NM_001077.3       GCAGTCTTCTGGATTGAGTTTGTCATGCGCCATAAAGGAGCCAAGCACCTTCGGGTCGCA
                  ******************************** ********************.******

HsCD00358265      GCTCACAACCTCACCTGGATCCAGTACCACTCTTTGGATTAG------------------
NM_001076.3       GCTCACAACCTCACCTGGATCCAGTACCACTCTTTGGATGTGATAGCATTCCTGCTGGCC
NM_001077.3       GCCCACAACCTCACCTGGATCCAGTACCACTCTTTGGATGTGATAGCATTCCTGCTGGCC
                  ** ************************************ :*                  

HsCD00358265      ------------------------------------------------------------
NM_001076.3       TGCGTGGCAACTGTGATATTTATCATCACAAAATTTTGCCTGTTTTGTTTCCGAAAGCTT
NM_001077.3       TGCGTGGCAACTATGATATTTATGATCACAAAATGTTGCCTGTTTTGTTTCCGAAAGCTT
                                                                              

HsCD00358265      ---------------------------------
NM_001076.3       GCCAAAAAAGGAAAGAAGAAGAAAAGAGATTAG
NM_001077.3       GCCAAAACAGGAAAGAAGAAGAAAAGGGATTAG
                                                   

Pathways

Reactome : Biological oxidations
Reactome : Glucuronidation
Reactome : Metabolism
Reactome : Phase II conjugation
WikiPathway : Glucuronidation

Protein

UniProt : P54855
HPRD : 07192

Clone

Cloning Information : 560371

Organism


TAX_ID : 9606
Species Specific ID: 7366

Genome

Chromosome : 4
Map Location : 4q13
Ensembl : ENSG00000196620

Reagents

Labome : UGT2B15-antibody