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LCTL (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LCTL
Gene Name:  lactase-like
Sequence             
Original Clone ID: None
Keyword: partial cds (N and C terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358266

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1548nts         Open reading frame : 1 to 1548
Reference Sequence Alignment

CCAGAAGAGGCCTCCTTCTACTATGGAACCTTCCCTCTTGGCTTCTCCTGGGGCGTGGGC
AGTTCTGCCTACCAGACGGAGGGCGCCTGGGACCAGGACGGGAAAGGGCCTAGCATCTGG
GACGTCTTCACACACAGTGGGAAGGGGAAAGTGCTTGGGAATGAGACGGCAGATGTAGCC
TGTGACGGCTACTACAAGGTCCAGGAGGACATCATTCTGCTGAGGGAACTGCACGTCAAC
CACTACCGATTCTCCCTGTCTTGGCCCCGGCTCCTGCCCACAGGCATCCGAGCCGAGCAG
GTGAACAAGAAGGGAATCGAATTCTACAGTGATCTTATCGATGCCCTTCTGAGCAGCAAC
ATCACTCCCATCGTGACCTTGCACCACTGGGATCTGCCACAGCTGCTCCAGGTCAAATAC
GGTGGGTGGCAGAATGTGAGCATGGCCAACTACTTCAGAGACTACGCCAACCTGTGCTTT
GAGGCCTTTGGGGACCGTGTGAAGCACTGGATCACGTTCAGTGATCCTCGGGCAATGGCA
GAAAAAGGCTATGAGACGGGCCACCATGCGCCGGGCCTGAAGCTCCGCGGCACCGGCCTG
TACAAGGCAGCACACCACATCATTAAGGCCCACGCCAAAGCCTGGCATTCTTATAACACC
ACGTGGCGCAGCAAGCAGCAAGGTCTGGTGGGAATTTCATTGAACTGTGACTGGGGGGAA
CCTGTGGACATTAGTAACCCCAAGGACCTAGAGGCTGCCGAGAGATACCTACAGTTCTGT
CTGGGCTGGTTTGCCAACCCCATTTATGCCGGTGACTACCCCCAAGTCATGAAGGACTAC
ATTGGAAGAAAGAGTGCAGAGCAAGGCCTGGAGATGTCGAGGTTACCGGTGTTCTCACTC
CAGGAGAAGAGCTACATTAAAGGCACATCCGATTTCTTGGGATTAGGTCATTTTACTACT
CGGTACATCACGGAAAGGAACTACCCCTCCCGCCAGGGGCCCAGCTACCAGAACGATCGT
GACTTGATAGAGCTGGTTGACCCAAACTGGCCAGATCTGGGGTCTAAATGGCTATATTCT
GTGCCATGGGGATTTAGGAGGCTCCTTAACTTTGCTCAGACTCAATACGGTGATCCTCCC
ATATATGTGATGGAAAATGGAGCATCTCAAAAATTCCACTGTACTCAATTATGTGATGAG
TGGAGAATTCAATACCTTAAAGGATACATAAATGAAATGCTAAAAGCTATAAAAGATGGT
GCTAATATAAAGGGGTATACTTCCTGGTCTCTGTTGGATAAGTTTGAATGGGAGAAAGGA
TACTCAGATAGATATGGATTCTACTATGTTGAATTTAACGACAGAAATAAGCCTCGCTAT
CCAAAGGCTTCAGTTCAATATTACAAGAAGATTATCATTGCCAATGGGTTTCCCAATCCA
AGAGAGGTGGAAAGTTGGTACCTCAAAGCTTTGGAAACTTGCTCTATCAACAATCAGATG
CTTGCTGCAGAGCCCTTGCTAAGTCACATGCAAATGGTTACGGAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,78; del@1627,75

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LCTL
Symbol Nomenclature : LCTL
SYNONYM : KLG; KLPH
Designation : KL lactase phlorizin hydrolase|klotho gamma|klotho/lactase-phlorizin hydrolase-related protein|lactase-like protein
Full Nomenclature : lactase-like
GENEID : 197021
Locus Tag : UNQ3022/PRO9820
GenBank Accession : BC148354
HGNC : 15583
Vega : OTTHUMG00000133207
Target GenBank: BC148354

 

Reference Sequence Alignment

  

HsCD00358266      ------------------------------------------------------------
NM_207338.3       ATGAAGCCAGTGTGGGTCGCCACCCTTCTGTGGATGCTACTGCTGGTGCCCAGGCTGGGG
                                                                              

HsCD00358266      ------------------CCAGAAGAGGCCTCCTTCTACTATGGAACCTTCCCTCTTGGC
NM_207338.3       GCCGCCCGGAAGGGGTCCCCAGAAGAGGCCTCCTTCTACTATGGAACCTTCCCTCTTGGC
                                    ******************************************

HsCD00358266      TTCTCCTGGGGCGTGGGCAGTTCTGCCTACCAGACGGAGGGCGCCTGGGACCAGGACGGG
NM_207338.3       TTCTCCTGGGGCGTGGGCAGTTCTGCCTACCAGACGGAGGGCGCCTGGGACCAGGACGGG
                  ************************************************************

HsCD00358266      AAAGGGCCTAGCATCTGGGACGTCTTCACACACAGTGGGAAGGGGAAAGTGCTTGGGAAT
NM_207338.3       AAAGGGCCTAGCATCTGGGACGTCTTCACACACAGTGGGAAGGGGAAAGTGCTTGGGAAT
                  ************************************************************

HsCD00358266      GAGACGGCAGATGTAGCCTGTGACGGCTACTACAAGGTCCAGGAGGACATCATTCTGCTG
NM_207338.3       GAGACGGCAGATGTAGCCTGTGACGGCTACTACAAGGTCCAGGAGGACATCATTCTGCTG
                  ************************************************************

HsCD00358266      AGGGAACTGCACGTCAACCACTACCGATTCTCCCTGTCTTGGCCCCGGCTCCTGCCCACA
NM_207338.3       AGGGAACTGCACGTCAACCACTACCGATTCTCCCTGTCTTGGCCCCGGCTCCTGCCCACA
                  ************************************************************

HsCD00358266      GGCATCCGAGCCGAGCAGGTGAACAAGAAGGGAATCGAATTCTACAGTGATCTTATCGAT
NM_207338.3       GGCATCCGAGCCGAGCAGGTGAACAAGAAGGGAATCGAATTCTACAGTGATCTTATCGAT
                  ************************************************************

HsCD00358266      GCCCTTCTGAGCAGCAACATCACTCCCATCGTGACCTTGCACCACTGGGATCTGCCACAG
NM_207338.3       GCCCTTCTGAGCAGCAACATCACTCCCATCGTGACCTTGCACCACTGGGATCTGCCACAG
                  ************************************************************

HsCD00358266      CTGCTCCAGGTCAAATACGGTGGGTGGCAGAATGTGAGCATGGCCAACTACTTCAGAGAC
NM_207338.3       CTGCTCCAGGTCAAATACGGTGGGTGGCAGAATGTGAGCATGGCCAACTACTTCAGAGAC
                  ************************************************************

HsCD00358266      TACGCCAACCTGTGCTTTGAGGCCTTTGGGGACCGTGTGAAGCACTGGATCACGTTCAGT
NM_207338.3       TACGCCAACCTGTGCTTTGAGGCCTTTGGGGACCGTGTGAAGCACTGGATCACGTTCAGT
                  ************************************************************

HsCD00358266      GATCCTCGGGCAATGGCAGAAAAAGGCTATGAGACGGGCCACCATGCGCCGGGCCTGAAG
NM_207338.3       GATCCTCGGGCAATGGCAGAAAAAGGCTATGAGACGGGCCACCATGCGCCGGGCCTGAAG
                  ************************************************************

HsCD00358266      CTCCGCGGCACCGGCCTGTACAAGGCAGCACACCACATCATTAAGGCCCACGCCAAAGCC
NM_207338.3       CTCCGCGGCACCGGCCTGTACAAGGCAGCACACCACATCATTAAGGCCCACGCCAAAGCC
                  ************************************************************

HsCD00358266      TGGCATTCTTATAACACCACGTGGCGCAGCAAGCAGCAAGGTCTGGTGGGAATTTCATTG
NM_207338.3       TGGCATTCTTATAACACCACGTGGCGCAGCAAGCAGCAAGGTCTGGTGGGAATTTCATTG
                  ************************************************************

HsCD00358266      AACTGTGACTGGGGGGAACCTGTGGACATTAGTAACCCCAAGGACCTAGAGGCTGCCGAG
NM_207338.3       AACTGTGACTGGGGGGAACCTGTGGACATTAGTAACCCCAAGGACCTAGAGGCTGCCGAG
                  ************************************************************

HsCD00358266      AGATACCTACAGTTCTGTCTGGGCTGGTTTGCCAACCCCATTTATGCCGGTGACTACCCC
NM_207338.3       AGATACCTACAGTTCTGTCTGGGCTGGTTTGCCAACCCCATTTATGCCGGTGACTACCCC
                  ************************************************************

HsCD00358266      CAAGTCATGAAGGACTACATTGGAAGAAAGAGTGCAGAGCAAGGCCTGGAGATGTCGAGG
NM_207338.3       CAAGTCATGAAGGACTACATTGGAAGAAAGAGTGCAGAGCAAGGCCTGGAGATGTCGAGG
                  ************************************************************

HsCD00358266      TTACCGGTGTTCTCACTCCAGGAGAAGAGCTACATTAAAGGCACATCCGATTTCTTGGGA
NM_207338.3       TTACCGGTGTTCTCACTCCAGGAGAAGAGCTACATTAAAGGCACATCCGATTTCTTGGGA
                  ************************************************************

HsCD00358266      TTAGGTCATTTTACTACTCGGTACATCACGGAAAGGAACTACCCCTCCCGCCAGGGGCCC
NM_207338.3       TTAGGTCATTTTACTACTCGGTACATCACGGAAAGGAACTACCCCTCCCGCCAGGGGCCC
                  ************************************************************

HsCD00358266      AGCTACCAGAACGATCGTGACTTGATAGAGCTGGTTGACCCAAACTGGCCAGATCTGGGG
NM_207338.3       AGCTACCAGAACGATCGTGACTTGATAGAGCTGGTTGACCCAAACTGGCCAGATCTGGGG
                  ************************************************************

HsCD00358266      TCTAAATGGCTATATTCTGTGCCATGGGGATTTAGGAGGCTCCTTAACTTTGCTCAGACT
NM_207338.3       TCTAAATGGCTATATTCTGTGCCATGGGGATTTAGGAGGCTCCTTAACTTTGCTCAGACT
                  ************************************************************

HsCD00358266      CAATACGGTGATCCTCCCATATATGTGATGGAAAATGGAGCATCTCAAAAATTCCACTGT
NM_207338.3       CAATACGGTGATCCTCCCATATATGTGATGGAAAATGGAGCATCTCAAAAATTCCACTGT
                  ************************************************************

HsCD00358266      ACTCAATTATGTGATGAGTGGAGAATTCAATACCTTAAAGGATACATAAATGAAATGCTA
NM_207338.3       ACTCAATTATGTGATGAGTGGAGAATTCAATACCTTAAAGGATACATAAATGAAATGCTA
                  ************************************************************

HsCD00358266      AAAGCTATAAAAGATGGTGCTAATATAAAGGGGTATACTTCCTGGTCTCTGTTGGATAAG
NM_207338.3       AAAGCTATAAAAGATGGTGCTAATATAAAGGGGTATACTTCCTGGTCTCTGTTGGATAAG
                  ************************************************************

HsCD00358266      TTTGAATGGGAGAAAGGATACTCAGATAGATATGGATTCTACTATGTTGAATTTAACGAC
NM_207338.3       TTTGAATGGGAGAAAGGATACTCAGATAGATATGGATTCTACTATGTTGAATTTAACGAC
                  ************************************************************

HsCD00358266      AGAAATAAGCCTCGCTATCCAAAGGCTTCAGTTCAATATTACAAGAAGATTATCATTGCC
NM_207338.3       AGAAATAAGCCTCGCTATCCAAAGGCTTCAGTTCAATATTACAAGAAGATTATCATTGCC
                  ************************************************************

HsCD00358266      AATGGGTTTCCCAATCCAAGAGAGGTGGAAAGTTGGTACCTCAAAGCTTTGGAAACTTGC
NM_207338.3       AATGGGTTTCCCAATCCAAGAGAGGTGGAAAGTTGGTACCTCAAAGCTTTGGAAACTTGC
                  ************************************************************

HsCD00358266      TCTATCAACAATCAGATGCTTGCTGCAGAGCCCTTGCTAAGTCACATGCAAATGGTTACG
NM_207338.3       TCTATCAACAATCAGATGCTTGCTGCAGAGCCCTTGCTAAGTCACATGCAAATGGTTACG
                  ************************************************************

HsCD00358266      GAGTAG------------------------------------------------------
NM_207338.3       GAGATCGTGGTACCCACTGTCTGCTCCCTCTGTGTCCTCATCACTGCTGTTCTACTAATG
                  ***::                                                       

HsCD00358266      ------------------------
NM_207338.3       CTCCTCCTGAGGAGGCAGAGCTGA
                                          

Protein

UniProt : Q6UWM7
UniProt : B3KQY0
HPRD : 13930

Clone

Cloning Information : 560379

Organism


TAX_ID : 9606
Species Specific ID: 197021

Genome

Chromosome : 15
Map Location : 15q22.31
Ensembl : ENSG00000188501

Reagents

Labome : LCTL-antibody