B3GALT1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  B3GALT1
Gene Name:  UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00358273
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 876nts
Open reading frame: 1 to 876

AAACCATTCAGCCACCTAACAGTTGCCAGGAAAAACTTCACCTTTGGCAACATAAGAACT
CGACCTATCAACCCACATTCTTTTGAATTTCTTATCAACGAGCCCAATAAATGTGAGAAA
AACATTCCTTTTCTTGTTATCCTCATCAGCACCACTCACAAGGAATTTGATGCCCGTCAG
GCAATCAGAGAGACGTGGGGGGATGAGAACAACTTTAAGGGGATCAAGATAGCCACCCTG
TTCCTCCTGGGCAAGAATGCTGATCCTGTTCTCAATCAGATGGTGGAGCAAGAGAGCCAA
ATCTTCCATGATATCATCGTGGAGGACTTTATTGACTCCTACCATAACCTTACCCTCAAA
ACATTAATGGGGATGAGATGGGTGGCCACTTTTTGTTCAAAAGCCAAGTATGTCATGAAA
ACAGACAGCGACATTTTTGTAAACATGGACAATCTTATTTATAAATTACTGAAACCCTCC
ACCAAGCCACGAAGAAGGTATTTTACTGGCTATGTCATTAATGGAGGACCGATTCGGGAT
GTCCGCAGTAAGTGGTATATGCCCAGGGATTTGTACCCAGACAGTAACTACCCACCTTTC
TGTTCGGGGACTGGCTACATCTTTTCAGCCGATGTAGCTGAACTCATTTACAAGACCTCA
CTCCACACAAGGCTGCTTCACCTTGAAGACGTATATGTGGGACTGTGTCTTCGAAAGCTG
GGCATACATCCTTTCCAGAACAGTGGCTTCAATCACTGGAAAATGGCCTACAGTTTGTGT
AGGTATCGCCGAGTTATCACTGTGCATCAGATCTCTCCAGAAGAAATGCACAGAATCTGG
AATGACATGTCAAGCAAGAAACATCTCAGATGTTAG

Protein sequence predicted based on nucleotide sequence

KPFSHLTVARKNFTFGNIRTRPINPHSFEFLINEPNKCEKNIPFLVILISTTHKEFDARQ
AIRETWGDENNFKGIKIATLFLLGKNADPVLNQMVEQESQIFHDIIVEDFIDSYHNLTLK
TLMGMRWVATFCSKAKYVMKTDSDIFVNMDNLIYKLLKPSTKPRRRYFTGYVINGGPIRD
VRSKWYMPRDLYPDSNYPPFCSGTGYIFSADVAELIYKTSLHTRLLHLEDVYVGLCLRKL
GIHPFQNSGFNHWKMAYSLCRYRRVITVHQISPEEMHRIWNDMSSKKHLRC*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,105

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable