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UGT2B10 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UGT2B10
Gene Name:  UDP glucuronosyltransferase 2 family, polypeptide B10
Sequence             
Original Clone ID: None
Keyword: partial cds (N and C terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358274

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1407nts         Open reading frame : 1 to 1407
Reference Sequence Alignment

AAGGTGCTGGTATGGGCCGCAGAATACAGCCTTTGGATGAATATGAAGACAATCCTGAAA
GAACTTGTTCAGAGAGGTCATGAGGTGACTGTACTGGCATCTTCAGCTTCCATTCTTTTT
GATCCCAACGACTCATCCACTCTTAAACTTGAAGTTTATCCTACATCTTTAACTAAAACT
GAATTTGAGAATATCATCATGCAATTGGTTAAGAGATTGTCAGAAATTCAAAAAGATACA
TTTTGGTTACCTTTTTCACAAGAACAAGAAATCCTGTGGGCAATTAATGACATAATTAGA
AACTTCTGTAAAGATGTAGTTTCAAATAAGAAACTTATGAAAAAACTACAAGAGTCAAGA
TTTGACATCGTTTTTGCAGATGCTTATTTACCCTGTGGTGAGCTGCTGGCTGAGCTATTT
AACATACCCTTTGTGTACAGTCACAGCTTCAGTCCTGGCTACTCATTTGAAAGGCACAGT
GGAGGATTTATTTTCCCTCCTTCCTACGTACCTGTTGTTATGTCAAAATTAAGTGATCAA
ATGACTTTCATGGAGAGGGTAAAAAATATGCTCTATGTGCTTTATTTTGACTTTTGGTTC
CAAATATTTAATATGAAGAAGTGGGATCAGTTTTACAGTGAAGTTTTAGGAAGACCCACT
ACATTATCTGAGACAATGAGGAAAGCTGACATATGGCTTATGCGAAACTCCTGGAATTTT
AAATTTCCTCATCCATTCTTACCAAATGTTGATTTTGTTGGAGGACTCCACTGCAAACCT
GCCAAACCCCTACCTAAGGAAATGGAGGAGTTTGTACAGAGCTCTGGAGAAAATGGTGTT
GTGGTGTTTTCTCTGGGGTCAATGGTCAGTAACATGACAGAAGAAAGGGCCAACGTAATT
GCAACAGCCCTTGCCAAGATCCCACAAAAGGTTCTTTGGAGATTTGATGGGAATAAACCA
GATGCCTTAGGTCTCAATACTCGACTGTACAAGTGGATACCCCAGAATGACCTTCTAGGT
CATCCAAAAACCAGAGCTTTTATAACTCATGGTGGAGCCAATGGCATCTATGAGGCAATC
TACCATGGGATCCCTATGGTGGGCATTCCATTGTTTTTTGATCAACCTGATAATATTGCT
CACATGAAGGCCAAGGGAGCAGCTGTTAGAGTGGACTTCAACACAATGTCGAGTACAGAC
CTGCTGAATGCACTGAAGACAGTAATTAATGATCCTTCATATAAAGAGAATATTATGAAA
TTATCAAGAATTCAACATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATT
GAATTTGTCATGCGCCACAAAGGAGCCAAACATCTTCGAGTTGCAGCCCACAACCTCACC
TGGTTCCAGTACCACTCTTTGGATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,69; del@1474,114

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UGT2B10
Symbol Nomenclature : UGT2B10
SYNONYM : UDPGT2B10
Designation : UDP glucuronosyltransferase 2B10|UDP-glucuronosyltransferase 2B10|UDPGT 2B10
Full Nomenclature : UDP glucuronosyltransferase 2 family, polypeptide B10
GENEID : 7365
GenBank Accession : BC113649
HGNC : 12544
MIM : 600070
Target GenBank: BC113649

 

Reference Sequence Alignment

  

HsCD00358274      ------------------------------------------------------------
NM_001073.1       ATGACTCTGAAATGGACTTCAGTTCTTCTGCTGATACATCTCAGTTGTTACTTTAGCTCT
                                                                              

HsCD00358274      ------------AAGGTGCTGGTATGGGCCGCAGAATACAGCCTTTGGATGAATATGAAG
NM_001073.1       GGGAGTTGTGGAAAAGTGCTGGTGTGGGCCGCAGAATACAGCCATTGGATGAATATGAAG
                              **.********.*******************:****************

HsCD00358274      ACAATCCTGAAAGAACTTGTTCAGAGAGGTCATGAGGTGACTGTACTGGCATCTTCAGCT
NM_001073.1       ACAATCCTGAAAGAGCTTGTTCAGAGAGGTCATGAGGTGACTGTACTGGCATCTTCAGCT
                  **************.*********************************************

HsCD00358274      TCCATTCTTTTTGATCCCAACGACTCATCCACTCTTAAACTTGAAGTTTATCCTACATCT
NM_001073.1       TCCATTCTTTTTGATCCCAATGATGCATCCACTCTTAAATTTGAAGTTTATCCTACATCT
                  ******************** **  ************** ********************

HsCD00358274      TTAACTAAAACTGAATTTGAGAATATCATCATGCAATTGGTTAAGAGATTGTCAGAAATT
NM_001073.1       TTAACTAAAACTGAATTTGAGAATATCATCATGCAACAGGTTAAGAGATGGTCAGACATT
                  ************************************ :*********** ******.***

HsCD00358274      CAAAAAGATACATTTTGGTTACCTTTTTCACAAGAACAAGAAATCCTGTGGGCAATTAAT
NM_001073.1       CGAAAAGATAGCTTTTGGTTATATTTTTCACAAGAACAAGAAATCCTGTGGGAATTATAT
                  *.******** .********* .*****************************.*:*::**

HsCD00358274      GACATAATTAGAAACTTCTGTAAAGATGTAGTTTCAAATAAGAAACTTATGAAAAAACTA
NM_001073.1       GACATATTTAGAAACTTCTGTAAAGATGTAGTTTCAAATAAGAAAGTTATGAAAAAACTA
                  ******:************************************** **************

HsCD00358274      CAAGAGTCAAGATTTGACATCGTTTTTGCAGATGCTTATTTACCCTGTGGTGAGCTGCTG
NM_001073.1       CAAGAGTCAAGATTTGACATCGTTTTTGCAGATGCTGTTTTTCCCTGTGGTGAGCTGCTG
                  ************************************ :***:******************

HsCD00358274      GCTGAGCTATTTAACATACCCTTTGTGTACAGTCACAGCTTCAGTCCTGGCTACTCATTT
NM_001073.1       GCTGCGCTACTTAACATACGGTTTGTGTACAGTCTCCGCTTTACTCCTGGCTACACAATT
                  ****.**** *********  *************:*.**** * **********:**:**

HsCD00358274      GAAAGGCACAGTGGAGGATTTATTTTCCCTCCTTCCTACGTACCTGTTGTTATGTCAAAA
NM_001073.1       GAAAGGCACAGTGGAGGACTGATTTTCCCTCCTTCCTACATACCTATTGTTATGTCAAAA
                  ****************** * ******************.*****.**************

HsCD00358274      TTAAGTGATCAAATGACTTTCATGGAGAGGGTAAAAAATATGCTCTATGTGCTTTATTTT
NM_001073.1       TTAAGTGATCAAATGACTTTCATGGAGAGGGTAAAAAATATGATCTATGTGCTTTATTTT
                  ******************************************.*****************

HsCD00358274      GACTTTTGGTTCCAAATATTTAATATGAAGAAGTGGGATCAGTTTTACAGTGAAGTTTTA
NM_001073.1       GACTTTTGGTTCCAAATGTCTGATATGAAGAAGTGGGATCAGTTTTACAGTGAAGTTTTA
                  *****************.* *.**************************************

HsCD00358274      GGAAGACCCACTACATTATCTGAGACAATGAGGAAAGCTGACATATGGCTTATGCGAAAC
NM_001073.1       GGAAGACCCACTACCTTATTTGAGACAATGGGAAAAGCTGACATATGGCTTATGCGAAAC
                  **************.**** **********.*.***************************

HsCD00358274      TCCTGGAATTTTAAATTTCCTCATCCATTCTTACCAAATGTTGATTTTGTTGGAGGACTC
NM_001073.1       TCCTGGAGTTTTCAATTTCCTCATCCATTCTTACCAAACGTTGATTTTGTTGGAGGATTC
                  *******.****.************************* ****************** **

HsCD00358274      CACTGCAAACCTGCCAAACCCCTACCTAAGGAAATGGAGGAGTTTGTACAGAGCTCTGGA
NM_001073.1       CACTGCAAACCTGCCAAACCCCTACCTAAGGAAATGGAGGAGTTTGTACAGAGCTCTGGA
                  ************************************************************

HsCD00358274      GAAAATGGTGTTGTGGTGTTTTCTCTGGGGTCAATGGTCAGTAACATGACAGAAGAAAGG
NM_001073.1       GAAAATGGTGTTGTGGTGTTTTCTCTGGGGTCAGTGATAAGTAACATGACAGCAGAAAGG
                  *********************************.**.*.*************.*******

HsCD00358274      GCCAACGTAATTGCAACAGCCCTTGCCAAGATCCCACAAAAGGTTCTTTGGAGATTTGAT
NM_001073.1       GCCAATGTAATTGCAACAGCCCTTGCCAAGATCCCACAAAAGGTTCTGTGGAGATTTGAC
                  ***** ***************************************** *********** 

HsCD00358274      GGGAATAAACCAGATGCCTTAGGTCTCAATACTCGACTGTACAAGTGGATACCCCAGAAT
NM_001073.1       GGGAATAAACCAGATGCCTTAGGTCTCAATACTCGGCTGTACAAGTGGATACCCCAGAAT
                  ***********************************.************************

HsCD00358274      GACCTTCTAGGTCATCCAAAAACCAGAGCTTTTATAACTCATGGTGGAGCCAATGGCATC
NM_001073.1       GACCTTCTAGGTCATCCAAAAACCAGAGCTTTTATAACTCATGGTGGAGCCAATGGCATC
                  ************************************************************

HsCD00358274      TATGAGGCAATCTACCATGGGATCCCTATGGTGGGCATTCCATTGTTTTTTGATCAACCT
NM_001073.1       TATGAGGCAATCTACCATGGGATCCCTATGGTGGGCATTCCATTGTTTTTTGATCAACCT
                  ************************************************************

HsCD00358274      GATAATATTGCTCACATGAAGGCCAAGGGAGCAGCTGTTAGAGTGGACTTCAACACAATG
NM_001073.1       GATAACATTGCTCACATGAAGGCCAAGGGAGCAGCTGTTAGATTGGACTTCAACACAATG
                  ***** ************************************ *****************

HsCD00358274      TCGAGTACAGACCTGCTGAATGCACTGAAGACAGTAATTAATGATCCTTCATATAAAGAG
NM_001073.1       TCGAGTACAGACCTGCTGAATGCACTGAAGACAGTAATTAATGATCCTTTATATAAAGAG
                  ************************************************* **********

HsCD00358274      AATATTATGAAATTATCAAGAATTCAACATGATCAACCAGTGAAGCCCCTGGATCGAGCA
NM_001073.1       AATATTATGAAATTATCAAGAATTCAACATGATCAACCAGTAAAGCCCCTGGATCGAGCA
                  *****************************************.******************

HsCD00358274      GTCTTCTGGATTGAATTTGTCATGCGCCACAAAGGAGCCAAACATCTTCGAGTTGCAGCC
NM_001073.1       GTCTTCTGGATTGAATTTGTCATGCCCCACAAAGGAGCCAAACACCTTCGAGTTGCAGCC
                  ************************* ****************** ***************

HsCD00358274      CACAACCTCACCTGGTTCCAGTACCACTCTTTGGATTAG---------------------
NM_001073.1       CATGACCTCACCTGGTTCCAGTACCACTCTTTGGATGTGATTGGGTTTCTGCTGGCCTGT
                  ** .******************************** :*                     

HsCD00358274      ------------------------------------------------------------
NM_001073.1       GTGGCAACTGTGATATTTATCATCACAAAGTTTTGTCTGTTTTGTTTCTGGAAGTTTGCT
                                                                              

HsCD00358274      ------------------------------
NM_001073.1       AGAAAAGGGAAGAAGGGAAAAAGAGATTAG
                                                

Pathways

Reactome : Biological oxidations
Reactome : Glucuronidation
Reactome : Metabolism
Reactome : Phase II conjugation
WikiPathway : Glucuronidation

Protein

UniProt : P36537
UniProt : B4DPP1
UniProt : Q53GU2
HPRD : 07193

Clone

Cloning Information : 560427

Organism


TAX_ID : 9606
Species Specific ID: 7365

Genome

Chromosome : 4
Map Location : 4q13.2

Reagents

Labome : UGT2B10-antibody