DNASU Plasmid Repository • 480.965.5697 | Email

A4GNT (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  A4GNT
Gene Name:  alpha-1,4-N-acetylglucosaminyltransferase
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358275

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 915nts         Open reading frame : 1 to 915
Reference Sequence Alignment

AAGTCCCACCAGGGGCTGGAAGCCCTCCTGAGCCACAGACGTGGCATTGTGTTTCTAGAG
ACCTCAGAGAGAATGGAGCCACCCCATTTGGTCTCCTGTTCCGTAGAGTCTGCTGCCAAG
ATTTATCCTGAGTGGCCTGTGGTGTTCTTTATGAAGGGTCTTACTGATTCCACACCGATG
CCCTCAAACTCCACATACCCAGCTTTTTCCTTCCTGTCAGCAATAGACAACGTTTTCCTC
TTCCCTTTGGATATGAAAAGGCTGCTTGAAGACACACCATTGTTTTCATGGTACAATCAA
ATCAACGCCAGCGCAGAGAGAAACTGGCTCCACATCAGCTCGGATGCATCCCGCCTGGCC
ATCATCTGGAAATACGGTGGCATCTACATGGACACCGATGTCATCTCCATCAGGCCCATC
CCTGAGGAGAACTTTTTGGCTGCGCAGGCTTCTCGGTACTCTAGTAATGGAATATTTGGG
TTCCTCCCCCACCACCCCTTTTTGTGGGAATGCATGGAAAACTTTGTTGAACACTATAAT
TCAGACATTTGGGGCAACCAAGGCCCTGAGTTGATGACAAGGATGTTGAGGGTATGGTGT
AAACTTGAAGACTTCCAGGAGGTGAGCGACCTCAGGTGTCTGAACATATCCTTCTTACAC
CCCCAAAGATTTTACCCCATCTCCTATCGAGAGTGGAGGCGCTACTATGAAGTGTGGGAT
ACAGAGCCAAGCTTCAATGTCTCTTATGCCCTGCATTTGTGGAACCACATGAACCAGGAG
GGGCGGGCTGTGATTAGAGGAAGCAACACACTGGTGGAAAATCTCTATCGCAAGCACTGT
CCCAGGACTTACAGGGACCTGATTAAAGGCCCAGAGGGGTCAGTGACTGGGGAGCTGGGT
CCAGGTAACAAATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,108

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : A4GNT
Symbol Nomenclature : A4GNT
SYNONYM : alpha4GnT
Full Nomenclature : alpha-1,4-N-acetylglucosaminyltransferase
GENEID : 51146
GenBank Accession : BC119639
HGNC : 17968
Vega : OTTHUMG00000159820
Target GenBank: BC119639

 

Reference Sequence Alignment

  

HsCD00358275      ------------------------------------------------------------
NM_016161.2       ATGCGGAAGGAGCTCCAGCTCTCCCTGTCAGTCACCTTGCTGCTTGTCTGTGGCTTCCTC
                                                                              

HsCD00358275      ------------------------------------------------AAGTCCCACCAG
NM_016161.2       TACCAGTTCACCCTGAAGTCCAGCTGCCTCTTCTGTTTGCCTTCTTTCAAGTCCCACCAG
                                                                  ************

HsCD00358275      GGGCTGGAAGCCCTCCTGAGCCACAGACGTGGCATTGTGTTTCTAGAGACCTCAGAGAGA
NM_016161.2       GGGCTGGAAGCCCTCCTGAGCCACAGACGTGGCATTGTGTTTCTAGAGACCTCAGAGAGA
                  ************************************************************

HsCD00358275      ATGGAGCCACCCCATTTGGTCTCCTGTTCCGTAGAGTCTGCTGCCAAGATTTATCCTGAG
NM_016161.2       ATGGAGCCACCCCATTTGGTCTCCTGTTCCGTAGAGTCTGCTGCCAAGATTTATCCTGAG
                  ************************************************************

HsCD00358275      TGGCCTGTGGTGTTCTTTATGAAGGGTCTTACTGATTCCACACCGATGCCCTCAAACTCC
NM_016161.2       TGGCCTGTGGTGTTCTTTATGAAGGGTCTTACTGATTCCACACCGATGCCCTCAAACTCC
                  ************************************************************

HsCD00358275      ACATACCCAGCTTTTTCCTTCCTGTCAGCAATAGACAACGTTTTCCTCTTCCCTTTGGAT
NM_016161.2       ACATACCCAGCTTTTTCCTTCCTGTCAGCAATAGACAACGTTTTCCTCTTCCCTTTGGAT
                  ************************************************************

HsCD00358275      ATGAAAAGGCTGCTTGAAGACACACCATTGTTTTCATGGTACAATCAAATCAACGCCAGC
NM_016161.2       ATGAAAAGGCTGCTTGAAGACACACCATTGTTTTCATGGTACAATCAAATCAACGCCAGC
                  ************************************************************

HsCD00358275      GCAGAGAGAAACTGGCTCCACATCAGCTCGGATGCATCCCGCCTGGCCATCATCTGGAAA
NM_016161.2       GCAGAGAGAAACTGGCTCCACATCAGCTCGGATGCATCCCGCCTGGCCATCATCTGGAAA
                  ************************************************************

HsCD00358275      TACGGTGGCATCTACATGGACACCGATGTCATCTCCATCAGGCCCATCCCTGAGGAGAAC
NM_016161.2       TACGGTGGCATCTACATGGACACCGATGTCATCTCCATCAGGCCCATCCCTGAGGAGAAC
                  ************************************************************

HsCD00358275      TTTTTGGCTGCGCAGGCTTCTCGGTACTCTAGTAATGGAATATTTGGGTTCCTCCCCCAC
NM_016161.2       TTTTTGGCTGCGCAGGCTTCTCGGTACTCTAGTAATGGAATATTTGGGTTCCTCCCCCAC
                  ************************************************************

HsCD00358275      CACCCCTTTTTGTGGGAATGCATGGAAAACTTTGTTGAACACTATAATTCAGACATTTGG
NM_016161.2       CACCCCTTTTTGTGGGAATGCATGGAAAACTTTGTTGAACACTATAATTCAGCCATTTGG
                  ****************************************************.*******

HsCD00358275      GGCAACCAAGGCCCTGAGTTGATGACAAGGATGTTGAGGGTATGGTGTAAACTTGAAGAC
NM_016161.2       GGCAACCAAGGCCCTGAGTTGATGACAAGGATGTTGAGGGTATGGTGTAAACTTGAAGAC
                  ************************************************************

HsCD00358275      TTCCAGGAGGTGAGCGACCTCAGGTGTCTGAACATATCCTTCTTACACCCCCAAAGATTT
NM_016161.2       TTCCAGGAGGTGAGCGACCTCAGGTGTCTGAACATATCCTTCTTACACCCCCAAAGATTT
                  ************************************************************

HsCD00358275      TACCCCATCTCCTATCGAGAGTGGAGGCGCTACTATGAAGTGTGGGATACAGAGCCAAGC
NM_016161.2       TACCCCATCTCCTATCGAGAGTGGAGGCGCTACTATGAAGTGTGGGATACAGAGCCAAGC
                  ************************************************************

HsCD00358275      TTCAATGTCTCTTATGCCCTGCATTTGTGGAACCACATGAACCAGGAGGGGCGGGCTGTG
NM_016161.2       TTCAATGTCTCTTATGCCCTGCATTTGTGGAACCACATGAACCAGGAGGGGCGGGCTGTG
                  ************************************************************

HsCD00358275      ATTAGAGGAAGCAACACACTGGTGGAAAATCTCTATCGCAAGCACTGTCCCAGGACTTAC
NM_016161.2       ATTAGAGGAAGCAACACACTGGTGGAAAATCTCTATCGCAAGCACTGTCCCAGGACTTAC
                  ************************************************************

HsCD00358275      AGGGACCTGATTAAAGGCCCAGAGGGGTCAGTGACTGGGGAGCTGGGTCCAGGTAACAAA
NM_016161.2       AGGGACCTGATTAAAGGCCCAGAGGGGTCAGTGACTGGGGAGCTGGGTCCAGGTAACAAA
                  ************************************************************

HsCD00358275      TAG
NM_016161.2       TAA
                  **.

Protein

UniProt : Q9UNA3
HPRD : 12390

Clone

Cloning Information : 560433

Organism


TAX_ID : 9606
Species Specific ID: 51146

Genome

Chromosome : 3
Map Location : 3p14.3
Ensembl : ENSG00000118017

Reagents

Labome : A4GNT-antibody