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ST8SIA5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ST8SIA5
Gene Name:  ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358277

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 927nts         Open reading frame : 1 to 927
Reference Sequence Alignment

GAAGTGAAGGTGCTGTCCATGGTGAAGCAGTCAGAGCTGTTCGACAGGTGGAAGAGCCTC
CAGATGTGCAAATGGGCGATGAACATCTCTGAGGCCAACCAGTTCAAGTCTACTCTGTCC
AGGTGCTGCAACGCCCCTGCCTTTCTCTTCACCACCCAGAAGAACACTCCCCTGGGGACA
AAGCTCAAGTATGAGGTGGACACCAGTGGCATCTACCACATCAACCAGGAGATCTTCCGC
ATGTTTCCCAAGGACATGCCCTACTACCGGTCCCAGTTTAAGAAGTGTGCTGTAGTGGGC
AACGGAGGCATCTTGAAGAACAGCCGCTGCGGGAGGGAGATCAACAGCGCCGACTTCGTC
TTCCGGTGCAACCTGCCCCCCATCTCAGAGAAGTACACCATGGATGTGGGGGTGAAGACG
GATGTGGTCACTGTGAACCCCAGCATCATCACAGAGAGGTTCCACAAGCTGGAGAAGTGG
CGGCGGCCGTTCTATCGCGTGCTGCAGGTGTACGAGAACGCGTCGGTGCTGCTGCCTGCC
TTCTACAACACGCGCAACACCGACGTGTCCATCCGCGTCAAGTACGTGCTGGACGACTTC
GAATCGCCGCAAGCTGTCTACTACTTCCATCCGCAGTACCTGGTCAACGTGTCGCGCTAC
TGGCTCAGCCTGGGGGTGCGCGCCAAGCGCATCAGCACCGGCCTCATTCTGGTCACTGCG
GCGCTGGAGCTCTGTGAGGAGGTGCACCTCTTTGGCTTCTGGGCCTTCCCCATGAACCCC
TCGGGCCTCTACATCACTCACCACTACTATGACAACGTCAAGCCGCGTCCCGGCTTCCAC
GCCATGCCCTCTGAGATCTTCAACTTCCTGCACTTGCACAGCCGAGGCATCCTCCGCGTG
CACACGGGCACCTGCAGCTGCTGCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ST8SIA5
Symbol Nomenclature : ST8SIA5
SYNONYM : SIAT8E; ST8SiaV
Designation : SIAT8-E|alpha-2,8-sialyltransferase 8E|sialyltransferase 8E (alpha-2, 8-polysialytransferase)|sialyltransferase St8Sia V|sialytransferase St8Sia V
Full Nomenclature : ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
GENEID : 29906
GenBank Accession : BC108910
HGNC : 17827
MIM : 607162
Vega : OTTHUMG00000132643
Target GenBank: BC108910

 

Reference Sequence Alignment

  

HsCD00358277        ------------------------------------------------------------
NM_013305.4         ATGCGCTACGCGGACCCCTCGGCCAACCGGGATTTGTTGGGGAGCCGAACTTTGCTCTTC
XM_005258250.1      ATGCGCTACGCGGACCCCTCGGCCAACCGGGATTTGTTGGGGAGCCGAACTTTGCTCTTC
                                                                                

HsCD00358277        ------------------------------------------------------------
NM_013305.4         ATCTTCATCTGCGCCTTTGCCTTGGTGACCTTGCTGCAACAGATCCTGTATGGCAGGAAC
XM_005258250.1      ATCTTCATCTGCGCCTTTGCCTTGGTGACCTTGCTGCAACAGATCCTGTATGGCAGGAAC
                                                                                

HsCD00358277        ------------------------------------------------------------
NM_013305.4         TACATTAAGAGG------------------------------------------------
XM_005258250.1      TACATTAAGAGAGGCCTCCAGTTTGGTTGGCAGAGTGGTGACCAGCTGACCAATTGGACG
                                                                                

HsCD00358277        ------------------------------------------------------------
NM_013305.4         ------------------------------------------------------------
XM_005258250.1      GGGCTCTTTAATGTCACGGATGATCCAGCAGTGCAGAGCGGCAGTGACTCCAGCTCCAGG
                                                                                

HsCD00358277        ------------------------------------------------------------
NM_013305.4         TACTTTGAATTTTATGAGGGGCCTTTTGAATATAACTCCACAAGATGCCTGGAGCTGAGG
XM_005258250.1      TACTTTGAATTTTATGAGGGGCCTTTTGAATATAACTCCACAAGATGCCTGGAGCTGAGG
                                                                                

HsCD00358277        ------------GAAGTGAAGGTGCTGTCCATGGTGAAGCAGTCAGAGCTGTTCGACAGG
NM_013305.4         CACGAAATATTGGAAGTGAAGGTGCTGTCCATGGTGAAGCAGTCAGAGCTGTTCGACAGG
XM_005258250.1      CACGAAATATTGGAAGTGAAGGTGCTGTCCATGGTGAAGCAGTCAGAGCTGTTCGACAGG
                                ************************************************

HsCD00358277        TGGAAGAGCCTCCAGATGTGCAAATGGGCGATGAACATCTCTGAGGCCAACCAGTTCAAG
NM_013305.4         TGGAAGAGCCTCCAGATGTGCAAATGGGCGATGAACATCTCTGAGGCCAACCAGTTCAAG
XM_005258250.1      TGGAAGAGCCTCCAGATGTGCAAATGGGCGATGAACATCTCTGAGGCCAACCAGTTCAAG
                    ************************************************************

HsCD00358277        TCTACTCTGTCCAGGTGCTGCAACGCCCCTGCCTTTCTCTTCACCACCCAGAAGAACACT
NM_013305.4         TCTACTCTGTCCAGGTGCTGCAACGCCCCTGCCTTTCTCTTCACCACCCAGAAGAACACT
XM_005258250.1      TCTACTCTGTCCAGGTGCTGCAACGCCCCTGCCTTTCTCTTCACCACCCAGAAGAACACT
                    ************************************************************

HsCD00358277        CCCCTGGGGACAAAGCTCAAGTATGAGGTGGACACCAGTGGCATCTACCACATCAACCAG
NM_013305.4         CCCCTGGGGACAAAGCTCAAGTATGAGGTGGACACCAGTGGCATCTACCACATCAACCAG
XM_005258250.1      CCCCTGGGGACAAAGCTCAAGTATGAGGTGGACACCAGTGGCATCTACCACATCAACCAG
                    ************************************************************

HsCD00358277        GAGATCTTCCGCATGTTTCCCAAGGACATGCCCTACTACCGGTCCCAGTTTAAGAAGTGT
NM_013305.4         GAGATCTTCCGCATGTTTCCCAAGGACATGCCCTACTACCGGTCCCAGTTTAAGAAGTGT
XM_005258250.1      GAGATCTTCCGCATGTTTCCCAAGGACATGCCCTACTACCGGTCCCAGTTTAAGAAGTGT
                    ************************************************************

HsCD00358277        GCTGTAGTGGGCAACGGAGGCATCTTGAAGAACAGCCGCTGCGGGAGGGAGATCAACAGC
NM_013305.4         GCTGTAGTGGGCAACGGAGGCATCTTGAAGAACAGCCGCTGCGGGAGGGAGATCAACAGC
XM_005258250.1      GCTGTAGTGGGCAACGGAGGCATCTTGAAGAACAGCCGCTGCGGGAGGGAGATCAACAGC
                    ************************************************************

HsCD00358277        GCCGACTTCGTCTTCCGGTGCAACCTGCCCCCCATCTCAGAGAAGTACACCATGGATGTG
NM_013305.4         GCCGACTTCGTCTTCCGGTGCAACCTGCCCCCCATCTCAGAGAAGTACACCATGGATGTG
XM_005258250.1      GCCGACTTCGTCTTCCGGTGCAACCTGCCCCCCATCTCAGAGAAGTACACCATGGATGTG
                    ************************************************************

HsCD00358277        GGGGTGAAGACGGATGTGGTCACTGTGAACCCCAGCATCATCACAGAGAGGTTCCACAAG
NM_013305.4         GGGGTGAAGACGGATGTGGTCACTGTGAACCCCAGCATCATCACAGAGAGGTTCCACAAG
XM_005258250.1      GGGGTGAAGACGGATGTGGTCACTGTGAACCCCAGCATCATCACAGAGAGGTTCCACAAG
                    ************************************************************

HsCD00358277        CTGGAGAAGTGGCGGCGGCCGTTCTATCGCGTGCTGCAGGTGTACGAGAACGCGTCGGTG
NM_013305.4         CTGGAGAAGTGGCGGCGGCCGTTCTATCGCGTGCTGCAGGTGTACGAGAACGCGTCGGTG
XM_005258250.1      CTGGAGAAGTGGCGGCGGCCGTTCTATCGCGTGCTGCAGGTGTACGAGAACGCGTCGGTG
                    ************************************************************

HsCD00358277        CTGCTGCCTGCCTTCTACAACACGCGCAACACCGACGTGTCCATCCGCGTCAAGTACGTG
NM_013305.4         CTGCTGCCTGCCTTCTACAACACGCGCAACACCGACGTGTCCATCCGCGTCAAGTACGTG
XM_005258250.1      CTGCTGCCTGCCTTCTACAACACGCGCAACACCGACGTGTCCATCCGCGTCAAGTACGTG
                    ************************************************************

HsCD00358277        CTGGACGACTTCGAATCGCCGCAAGCTGTCTACTACTTCCATCCGCAGTACCTGGTCAAC
NM_013305.4         CTGGACGACTTCGAATCGCCGCAAGCTGTCTACTACTTCCATCCGCAGTACCTGGTCAAC
XM_005258250.1      CTGGACGACTTCGAATCGCCGCAAGCTGTCTACTACTTCCATCCGCAGTACCTGGTCAAC
                    ************************************************************

HsCD00358277        GTGTCGCGCTACTGGCTCAGCCTGGGGGTGCGCGCCAAGCGCATCAGCACCGGCCTCATT
NM_013305.4         GTGTCGCGCTACTGGCTCAGCCTGGGGGTGCGCGCCAAGCGCATCAGCACCGGCCTCATT
XM_005258250.1      GTGTCGCGCTACTGGCTCAGCCTGGGGGTGCGCGCCAAGCGCATCAGCACCGGCCTCATT
                    ************************************************************

HsCD00358277        CTGGTCACTGCGGCGCTGGAGCTCTGTGAGGAGGTGCACCTCTTTGGCTTCTGGGCCTTC
NM_013305.4         CTGGTCACTGCGGCGCTGGAGCTCTGTGAGGAGGTGCACCTCTTTGGCTTCTGGGCCTTC
XM_005258250.1      CTGGTCACTGCGGCGCTGGAGCTCTGTGAGGAGGTGCACCTCTTTGGCTTCTGGGCCTTC
                    ************************************************************

HsCD00358277        CCCATGAACCCCTCGGGCCTCTACATCACTCACCACTACTATGACAACGTCAAGCCGCGT
NM_013305.4         CCCATGAACCCCTCGGGCCTCTACATCACTCACCACTACTATGACAACGTCAAGCCGCGT
XM_005258250.1      CCCATGAACCCCTCGGGCCTCTACATCACTCACCACTACTATGACAACGTCAAGCCGCGT
                    ************************************************************

HsCD00358277        CCCGGCTTCCACGCCATGCCCTCTGAGATCTTCAACTTCCTGCACTTGCACAGCCGAGGC
NM_013305.4         CCCGGCTTCCACGCCATGCCCTCTGAGATCTTCAACTTCCTGCACTTGCACAGCCGAGGC
XM_005258250.1      CCCGGCTTCCACGCCATGCCCTCTGAGATCTTCAACTTCCTGCACTTGCACAGCCGAGGC
                    ************************************************************

HsCD00358277        ATCCTCCGCGTGCACACGGGCACCTGCAGCTGCTGCTAG
NM_013305.4         ATCCTCCGCGTGCACACGGGCACCTGCAGCTGCTGCTGA
XM_005258250.1      ATCCTCCGCGTGCACACGGGCACCTGCAGCTGCTGCTGA
                    *************************************..

Pathways

Reactome : Asparagine N-linked glycosylation
Reactome : Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome : Metabolism of proteins
Reactome : Post-translational protein modification
Reactome : Sialic acid metabolism
Reactome : Synthesis of substrates in N-glycan biosythesis
WikiPathway : Ganglio Sphingolipid Metabolism

Protein

UniProt : O15466
UniProt : B3KSU2
HPRD : 06199

Clone

Cloning Information : 560442

Organism


TAX_ID : 9606
Species Specific ID: 29906

Genome

Chromosome : 18
Map Location : 18q21.1
Ensembl : ENSG00000101638

Reagents

Labome : ST8SIA5-antibody