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B3GALT5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  B3GALT5
Gene Name:  UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
Sequence             
Original Clone ID: None
Keyword: full-length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358278

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 933nts         Open reading frame : 1 to 933
Reference Sequence Alignment

ATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTGGGGGCTCTTTGT
TTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTTGTTTACAAGAAA
GACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCTCCCTTCCTCGTC
CTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATCCGGCAGACGTGG
GGGAAAGAGAGGACGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTCCTGGGGACCACC
AGCAGTGCAGCGGAAACAAAAGAGGTGGACCAGGAGAGCCAGCGACACGGGGACATTATC
CAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATGATGGGCATAGAA
TGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGACTCAGACATGTTC
ATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACAACCAGGTTTTTC
ACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGACAGCCATTCAGCAAGTGGTTTGTC
AGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGCACCGGCTACGTG
TTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTCCCATACATTAAA
CTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGATTGGAGGAGCTC
CACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGCCTCTTCAGGAGG
ATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGGCAGGCTCTAGAG
AATTCCCGGGGGGAAGATTGTCCGCCTGTCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : B3GALT5
Symbol Nomenclature : B3GALT5
SYNONYM : B3GalT-V; B3GalTx; B3T5; GLCT5; beta-1,3-GalTase 5; beta-3-Gx-T5; beta3Gal-T5
Designation : UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5|beta-1,3-galactosyltransferase 5|beta-3-galactosyltransferase 5|homolog of C. elegans Bt toxin resistance gene bre-5
Full Nomenclature : UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
GENEID : 10317
GenBank Accession : BC104862
HGNC : 920
MIM : 604066
Vega : OTTHUMG00000086725
Target GenBank: BC104862

 

Reference Sequence Alignment

  

HsCD00358278        ------------ATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTG
NM_033170.2         ------------ATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTG
NM_001278650.1      ------------ATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTG
NM_006057.2         ------------ATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTG
NM_033172.2         ATGTGTTTGGAGATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTG
XM_005260912.1      ------------ATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTG
NM_033171.2         ------------ATGGCTTTCCCGAAGATGAGATTGATGTATATTTGCCTTCTGGTTCTG
                                ************************************************

HsCD00358278        GGGGCTCTTTGTTTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTT
NM_033170.2         GGGGCTCTTTGTTTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTT
NM_001278650.1      GGGGCTCTTTGTTTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTT
NM_006057.2         GGGGCTCTTTGTTTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTT
NM_033172.2         GGGGCTCTTTGTTTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTT
XM_005260912.1      GGGGCTCTTTGTTTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTT
NM_033171.2         GGGGCTCTTTGTTTGTATTTTAGCATGTACAGTCTAAATCCTTTCAAAGAACAGTCCTTT
                    ************************************************************

HsCD00358278        GTTTACAAGAAAGACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCT
NM_033170.2         GTTTACAAGAAAGACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCT
NM_001278650.1      GTTTACAAGAAAGACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCT
NM_006057.2         GTTTACAAGAAAGACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCT
NM_033172.2         GTTTACAAGAAAGACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCT
XM_005260912.1      GTTTACAAGAAAGACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCT
NM_033171.2         GTTTACAAGAAAGACGGGAACTTCCTTAAGCTCCCAGATACAGACTGCAGGCAGACACCT
                    ************************************************************

HsCD00358278        CCCTTCCTCGTCCTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATC
NM_033170.2         CCCTTCCTCGTCCTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATC
NM_001278650.1      CCCTTCCTCGTCCTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATC
NM_006057.2         CCCTTCCTCGTCCTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATC
NM_033172.2         CCCTTCCTCGTCCTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATC
XM_005260912.1      CCCTTCCTCGTCCTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATC
NM_033171.2         CCCTTCCTCGTCCTGCTGGTGACCTCATCCCACAAACAGTTGGCTGAGCGCATGGCCATC
                    ************************************************************

HsCD00358278        CGGCAGACGTGGGGGAAAGAGAGGACGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTC
NM_033170.2         CGGCAGACGTGGGGGAAAGAGAGGATGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTC
NM_001278650.1      CGGCAGACGTGGGGGAAAGAGAGGATGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTC
NM_006057.2         CGGCAGACGTGGGGGAAAGAGAGGATGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTC
NM_033172.2         CGGCAGACGTGGGGGAAAGAGAGGATGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTC
XM_005260912.1      CGGCAGACGTGGGGGAAAGAGAGGATGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTC
NM_033171.2         CGGCAGACGTGGGGGAAAGAGAGGATGGTGAAGGGAAAGCAGCTGAAGACATTCTTCCTC
                    ************************* **********************************

HsCD00358278        CTGGGGACCACCAGCAGTGCAGCGGAAACAAAAGAGGTGGACCAGGAGAGCCAGCGACAC
NM_033170.2         CTGGGGACCACCAGCAGTGCAGCGGAAACGAAAGAGGTGGACCAGGAGAGCCAGCGACAC
NM_001278650.1      CTGGGGACCACCAGCAGTGCAGCGGAAACGAAAGAGGTGGACCAGGAGAGCCAGCGACAC
NM_006057.2         CTGGGGACCACCAGCAGTGCAGCGGAAACGAAAGAGGTGGACCAGGAGAGCCAGCGACAC
NM_033172.2         CTGGGGACCACCAGCAGTGCAGCGGAAACGAAAGAGGTGGACCAGGAGAGCCAGCGACAC
XM_005260912.1      CTGGGGACCACCAGCAGTGCAGCGGAAACGAAAGAGGTGGACCAGGAGAGCCAGCGACAC
NM_033171.2         CTGGGGACCACCAGCAGTGCAGCGGAAACGAAAGAGGTGGACCAGGAGAGCCAGCGACAC
                    *****************************.******************************

HsCD00358278        GGGGACATTATCCAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATG
NM_033170.2         GGGGACATTATCCAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATG
NM_001278650.1      GGGGACATTATCCAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATG
NM_006057.2         GGGGACATTATCCAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATG
NM_033172.2         GGGGACATTATCCAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATG
XM_005260912.1      GGGGACATTATCCAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATG
NM_033171.2         GGGGACATTATCCAGAAGGATTTCCTAGACGTCTATTACAATCTGACCCTGAAGACCATG
                    ************************************************************

HsCD00358278        ATGGGCATAGAATGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGAC
NM_033170.2         ATGGGCATAGAATGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGAC
NM_001278650.1      ATGGGCATAGAATGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGAC
NM_006057.2         ATGGGCATAGAATGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGAC
NM_033172.2         ATGGGCATAGAATGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGAC
XM_005260912.1      ATGGGCATAGAATGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGAC
NM_033171.2         ATGGGCATAGAATGGGTCCATCGCTTTTGTCCTCAGGCGGCGTTTGTGATGAAAACAGAC
                    ************************************************************

HsCD00358278        TCAGACATGTTCATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACA
NM_033170.2         TCAGACATGTTCATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACA
NM_001278650.1      TCAGACATGTTCATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACA
NM_006057.2         TCAGACATGTTCATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACA
NM_033172.2         TCAGACATGTTCATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACA
XM_005260912.1      TCAGACATGTTCATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACA
NM_033171.2         TCAGACATGTTCATCAATGTTGACTATCTGACTGAACTGCTTCTGAAGAAAAACAGAACA
                    ************************************************************

HsCD00358278        ACCAGGTTTTTCACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGACAGCCATTCAGC
NM_033170.2         ACCAGGTTTTTCACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGGCAGCCATTCAGC
NM_001278650.1      ACCAGGTTTTTCACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGGCAGCCATTCAGC
NM_006057.2         ACCAGGTTTTTCACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGGCAGCCATTCAGC
NM_033172.2         ACCAGGTTTTTCACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGGCAGCCATTCAGC
XM_005260912.1      ACCAGGTTTTTCACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGGCAGCCATTCAGC
NM_033171.2         ACCAGGTTTTTCACTGGCTTCTTGAAACTCAATGAGTTTCCCATCAGGCAGCCATTCAGC
                    ***********************************************.************

HsCD00358278        AAGTGGTTTGTCAGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGC
NM_033170.2         AAGTGGTTTGTCAGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGC
NM_001278650.1      AAGTGGTTTGTCAGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGC
NM_006057.2         AAGTGGTTTGTCAGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGC
NM_033172.2         AAGTGGTTTGTCAGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGC
XM_005260912.1      AAGTGGTTTGTCAGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGC
NM_033171.2         AAGTGGTTTGTCAGTAAATCTGAATATCCGTGGGACAGGTACCCACCATTCTGCTCCGGC
                    ************************************************************

HsCD00358278        ACCGGCTACGTGTTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTC
NM_033170.2         ACCGGCTACGTGTTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTC
NM_001278650.1      ACCGGCTACGTGTTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTC
NM_006057.2         ACCGGCTACGTGTTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTC
NM_033172.2         ACCGGCTACGTGTTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTC
XM_005260912.1      ACCGGCTACGTGTTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTC
NM_033171.2         ACCGGCTACGTGTTTTCTGGCGACGTGGCGAGTCAGGTGTACAATGTCTCCAAGAGCGTC
                    ************************************************************

HsCD00358278        CCATACATTAAACTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGA
NM_033170.2         CCATACATTAAACTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGA
NM_001278650.1      CCATACATTAAACTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGA
NM_006057.2         CCATACATTAAACTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGA
NM_033172.2         CCATACATTAAACTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGA
XM_005260912.1      CCATACATTAAACTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGA
NM_033171.2         CCATACATTAAACTGGAAGACGTGTTTGTGGGGCTCTGCCTCGAAAGGCTGAACATCAGA
                    ************************************************************

HsCD00358278        TTGGAGGAGCTCCACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGC
NM_033170.2         TTGGAGGAGCTCCACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGC
NM_001278650.1      TTGGAGGAGCTCCACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGC
NM_006057.2         TTGGAGGAGCTCCACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGC
NM_033172.2         TTGGAGGAGCTCCACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGC
XM_005260912.1      TTGGAGGAGCTCCACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGC
NM_033171.2         TTGGAGGAGCTCCACTCCCAGCCGACCTTTTTTCCAGGGGGCTTACGCTTCTCCGTATGC
                    ************************************************************

HsCD00358278        CTCTTCAGGAGGATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGG
NM_033170.2         CTCTTCAGGAGGATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGG
NM_001278650.1      CTCTTCAGGAGGATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGG
NM_006057.2         CTCTTCAGGAGGATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGG
NM_033172.2         CTCTTCAGGAGGATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGG
XM_005260912.1      CTCTTCAGGAGGATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGG
NM_033171.2         CTCTTCAGGAGGATCGTGGCCTGCCACTTCATCAAGCCTCGGACTCTCTTGGACTACTGG
                    ************************************************************

HsCD00358278        CAGGCTCTAGAGAATTCCCGGGGGGAAGATTGTCCGCCTGTCTAG
NM_033170.2         CAGGCTCTAGAGAATTCCCGGGGGGAAGATTGTCCGCCTGTCTGA
NM_001278650.1      CAGGCTCTAGAGAATTCCCGGGGGGAAGATTGTCCGCCTGTCTGA
NM_006057.2         CAGGCTCTAGAGAATTCCCGGGGGGAAGATTGTCCGCCTGTCTGA
NM_033172.2         CAGGCTCTAGAGAATTCCCGGGGGGAAGATTGTCCGCCTGTCTGA
XM_005260912.1      CAGGCTCTAGAGAATTCCCGGGGGGAAGATTGTCCGCCTGTCTGA
NM_033171.2         CAGGCTCTAGAGAATTCCCGGGGGGAAGATTGTCCGCCTGTCTGA
                    *******************************************..

Protein

UniProt : Q9Y2C3
HPRD : 06818

Clone

Cloning Information : 560450

Organism


TAX_ID : 9606
Species Specific ID: 10317

Genome

Chromosome : 21
Map Location : 21q22.3
Ensembl : ENSG00000183778

Reagents

Labome : B3GALT5-antibody