DNASU Plasmid Repository • 480.965.5697 | Email

ST8SIA2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ST8SIA2
Gene Name:  ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358279

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 951nts         Open reading frame : 1 to 951
Reference Sequence Alignment

AACGGCTCCTCATCACCAGCTGTTGTTGACAGAAGTAATGAAAGCATCAAGCACAACATC
CAGCCAGCCTCGTCCAAATGGAGACATAACCAGACGCTCTCTCTGAGGATCAGGAAGCAG
ATTTTAAAGTTCTTGGATGCTGAAAAGGACATTTCTGTCCTAAAGGGAACCCTGAAGCCT
GGAGATATTATTCATTACATCTTCGATCGAGACAGCACCATGAATGTGTCCCAGAACCTC
TACGAGCTCCTCCCCAGGACTTCGCCACTGAAGAATAAGCACTTTGGGACTTGTGCCATC
GTGGGCAACTCGGGGGTCTTGCTGAACAGCGGCTGTGGGCAGGAGATTGACGCCCACAGC
TTCGTCATCAGGTGCAACCTGGCCCCAGTACAGGAGTATGCCCGGGATGTGGGGCTCAAG
ACAGACCTGGTAACCATGAACCCGTCGGTCATCCAGCGGGCCTTTGAGGACTTGGTCAAT
GCCACGTGGCGGGAGAAGCTGCTGCAACGGCTGCACAGCCTCAATGGCAGCATCCTGTGG
ATCCCTGCCTTCATGGCCCGGGGCGGCAAGGAGCGTGTTGAGTGGGTCAACGAGCTTATC
CTGAAGCACCACGTCAACGTGCGCACTGCATACCCCTCGCTGCGCCTGCTGCACGCCGTT
CGCGGATACTGGCTGACCAACAAAGTCCACATCAAAAGACCCACCACCGGCCTCTTGATG
TATACCCTGGCCACACGTTTCTGCAAACAAATCTACCTCTACGGCTTCTGGCCCTTTCCG
CTGGATCAGAACCAGAACCCAGTCAAGTACCACTATTATGACAGCCTCAAGTATGGCTAC
ACCTCCCAGGCCAGCGCGCATACCATGCCCTTGGAGTTTAAGGCCCTCAAGAGCCTACAT
GAGCAGGGGGCTTTGAAACTGACTGTCGGCCAGTGCGATGGGGCCACGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ST8SIA2
Symbol Nomenclature : ST8SIA2
SYNONYM : HsT19690; SIAT8B; ST8SIA-II; STX
Designation : SIAT8-B|ST8SiaII|alpha-2,8-sialyltransferase 8B|alpha-2,8-sialyltransferase 8B 1|sialyltransferase 8 (alpha-2, 8-sialytransferase) B|sialyltransferase 8B|sialyltransferase St8Sia II|sialyltransferase X|sialytransferase St8Sia II
Full Nomenclature : ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
GENEID : 8128
Locus Tag : UNQ3057
GenBank Accession : BC096202
HGNC : 10870
MIM : 602546
Vega : OTTHUMG00000149843
Target GenBank: BC096202

 

Reference Sequence Alignment

  

HsCD00358279      ------------------------------------------------------------
NM_006011.3       ATGCAGCTGCAGTTCCGGAGCTGGATGCTGGCCGCGCTCACGCTGCTCGTGGTCTTCCTC
                                                                              

HsCD00358279      ------------------------------------------------------------
NM_006011.3       ATCTTCGCAGACATCTCAGAGATCGAAGAAGAAATCGGGAATTCGGGAGGCAGAGGTACA
                                                                              

HsCD00358279      ---------------------------------------------------------AAC
NM_006011.3       ATCAGATCAGCTGTGAACAGCTTACATAGCAAATCTAATAGAGCTGAAGTTGTAATAAAC
                                                                           ***

HsCD00358279      GGCTCCTCATCACCAGCTGTTGTTGACAGAAGTAATGAAAGCATCAAGCACAACATCCAG
NM_006011.3       GGCTCCTCATCACCAGCTGTTGTTGACAGAAGTAATGAAAGCATCAAGCACAACATCCAG
                  ************************************************************

HsCD00358279      CCAGCCTCGTCCAAATGGAGACATAACCAGACGCTCTCTCTGAGGATCAGGAAGCAGATT
NM_006011.3       CCAGCCTCGTCCAAATGGAGACATAACCAGACGCTCTCTCTGAGGATCAGGAAGCAGATT
                  ************************************************************

HsCD00358279      TTAAAGTTCTTGGATGCTGAAAAGGACATTTCTGTCCTAAAGGGAACCCTGAAGCCTGGA
NM_006011.3       TTAAAGTTCTTGGATGCTGAAAAGGACATTTCTGTCCTAAAGGGAACCCTGAAGCCTGGA
                  ************************************************************

HsCD00358279      GATATTATTCATTACATCTTCGATCGAGACAGCACCATGAATGTGTCCCAGAACCTCTAC
NM_006011.3       GATATTATTCATTACATCTTCGATCGAGACAGCACCATGAATGTGTCCCAGAACCTCTAC
                  ************************************************************

HsCD00358279      GAGCTCCTCCCCAGGACTTCGCCACTGAAGAATAAGCACTTTGGGACTTGTGCCATCGTG
NM_006011.3       GAGCTCCTCCCCAGGACTTCGCCACTGAAGAATAAGCACTTTGGGACTTGTGCCATCGTG
                  ************************************************************

HsCD00358279      GGCAACTCGGGGGTCTTGCTGAACAGCGGCTGTGGGCAGGAGATTGACGCCCACAGCTTC
NM_006011.3       GGCAACTCGGGGGTCTTGCTGAACAGCGGCTGTGGGCAGGAGATTGACGCCCACAGCTTC
                  ************************************************************

HsCD00358279      GTCATCAGGTGCAACCTGGCCCCAGTACAGGAGTATGCCCGGGATGTGGGGCTCAAGACA
NM_006011.3       GTCATCAGGTGCAACCTGGCCCCAGTACAGGAGTATGCCCGGGATGTGGGGCTCAAGACA
                  ************************************************************

HsCD00358279      GACCTGGTAACCATGAACCCGTCGGTCATCCAGCGGGCCTTTGAGGACTTGGTCAATGCC
NM_006011.3       GACCTGGTAACCATGAACCCCTCGGTCATCCAGCGGGCCTTTGAGGACTTGGTCAATGCC
                  ******************** ***************************************

HsCD00358279      ACGTGGCGGGAGAAGCTGCTGCAACGGCTGCACAGCCTCAATGGCAGCATCCTGTGGATC
NM_006011.3       ACGTGGCGGGAGAAGCTGCTGCAACGGCTGCACAGCCTCAATGGCAGCATCCTGTGGATC
                  ************************************************************

HsCD00358279      CCTGCCTTCATGGCCCGGGGCGGCAAGGAGCGTGTTGAGTGGGTCAACGAGCTTATCCTG
NM_006011.3       CCTGCCTTCATGGCCCGGGGCGGCAAGGAGCGTGTTGAGTGGGTCAACGAGCTTATCCTG
                  ************************************************************

HsCD00358279      AAGCACCACGTCAACGTGCGCACTGCATACCCCTCGCTGCGCCTGCTGCACGCCGTTCGC
NM_006011.3       AAGCACCACGTCAACGTGCGCACTGCATACCCCTCGCTGCGCCTGCTGCACGCCGTTCGC
                  ************************************************************

HsCD00358279      GGATACTGGCTGACCAACAAAGTCCACATCAAAAGACCCACCACCGGCCTCTTGATGTAT
NM_006011.3       GGATACTGGCTGACCAACAAAGTCCACATCAAAAGACCCACCACCGGCCTCTTGATGTAT
                  ************************************************************

HsCD00358279      ACCCTGGCCACACGTTTCTGCAAACAAATCTACCTCTACGGCTTCTGGCCCTTTCCGCTG
NM_006011.3       ACCCTGGCCACACGTTTCTGCAAACAAATCTACCTCTACGGCTTCTGGCCCTTTCCGCTG
                  ************************************************************

HsCD00358279      GATCAGAACCAGAACCCAGTCAAGTACCACTATTATGACAGCCTCAAGTATGGCTACACC
NM_006011.3       GATCAGAACCAGAACCCAGTCAAGTACCACTATTATGACAGCCTCAAGTATGGCTACACC
                  ************************************************************

HsCD00358279      TCCCAGGCCAGCGCGCATACCATGCCCTTGGAGTTTAAGGCCCTCAAGAGCCTACATGAG
NM_006011.3       TCCCAGGCCAGCCCGCATACCATGCCCTTGGAGTTTAAGGCCCTCAAGAGCCTACATGAG
                  ************ ***********************************************

HsCD00358279      CAGGGGGCTTTGAAACTGACTGTCGGCCAGTGCGATGGGGCCACGTAG
NM_006011.3       CAGGGGGCTTTGAAACTGACTGTCGGCCAGTGCGATGGGGCCACGTAG
                  ************************************************

Pathways

Reactome : Asparagine N-linked glycosylation
Reactome : Axon guidance
Reactome : Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome : Developmental Biology
Reactome : Metabolism of proteins
Reactome : N-Glycan antennae elongation
Reactome : N-glycan antennae elongation in the medial/trans-Golgi
Reactome : NCAM signaling for neurite out-growth
Reactome : NCAM1 interactions
Reactome : Post-translational protein modification
Reactome : Sialic acid metabolism
Reactome : Synthesis of substrates in N-glycan biosythesis
Reactome : Transport to the Golgi and subsequent modification

Protein

UniProt : B2R9U8
UniProt : Q92186
HPRD : 03969

Clone

Cloning Information : 560454

Organism


TAX_ID : 9606
Species Specific ID: 8128

Genome

Chromosome : 15
Map Location : 15q26
Ensembl : ENSG00000140557

Reagents

Labome : STX-antibody