DNASU Plasmid Repository • 480.965.5697 | Email

GLT8D1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  GLT8D1
Gene Name:  glycosyltransferase 8 domain containing 1
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358283

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 963nts         Open reading frame : 1 to 963
Reference Sequence Alignment

CCAAATGCTCTCCGACATGCAGTAGATGGGAGACAAGAGGAGATTCCTGTGGTCATCGCT
GCATCTGAAGACAGGCTTGGGGGGGCCATTGCAGCTATAAACAGCATTCAGCACAACACT
CGCTCCAATGTGATTTTCTACATTGTTACTCTCAACAATACAGCAGACCATCTCCGGTCC
TGGCTCAACAGTGATTCCCTGAAAAGCATCAGATACAAAATTGTCAATTTTGACCCTAAA
CTTTTGGAAGGAAAAGTAAAGGAGGATCCTGACCAGGGGGAATCCATGAAACCTTTAACC
TTTGCAAGGTTCTACTTGCCAATTCTGGTTCCCAGCGCAAAGAAGGCCATATACATGGAT
GATGATGTAATTGTGCAAGGTGATATTCTTGCCCTTTACAATACAGCACTGAAGCCAGGA
CATGCAGCTGCATTTTCAGAAGATTGTGATTCAGCCTCTACTAAAGTTGTCATCCGTGGA
GCAGGAAACCAGTACAATTACATTGGCTATCTTGACTATAAAAAGGAAAGAATTCGTAAG
CTTTCCATGAAAGCCAGCACTTGCTCATTTAATCCTGGAGTTTTTGTTGCAAACCTGACG
GAATGGAAACGACAGAATATAACTAACCAACTGGAAAAATGGATGAAACTCAATGTAGAA
GAGGGACTGTATAGCAGAACCCTGGCTGGTAGCATCACAACACCTCCTCTGCTTATCGTA
TTTTATCAACAGCACTCTACCATCGATCCTATGTGGAATGTCCGCCACCTTGGTTCCAGT
GCTGGAAAACGATATTCACCTCAGTTTGTAAAGGCTGCCAAGTTACTCCATTGGAATGGA
CATTTGAAGCCATGGGGAAGGACTGCTTCATATACTGATGTTTGGGAAAAATGGTATATT
CCAGACCCAACAGGCAAATTCAACCTAATCCGAAGATATACCGAGATCTCAAACATAAAG
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,153

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GLT8D1
Symbol Nomenclature : GLT8D1
SYNONYM : MSTP139
Designation : glycosyltransferase 8 domain-containing protein 1|glycosyltransferase AD-017
Full Nomenclature : glycosyltransferase 8 domain containing 1
GENEID : 55830
Locus Tag : AD-017
GenBank Accession : BC119670
HGNC : 24870
Vega : OTTHUMG00000158759
Target GenBank: BC119670

 

Reference Sequence Alignment

  

HsCD00358283        ------------------------------------------------------------
NM_001278280.1      ATGTCATTCCGTAAAGTAAACATCATCATCTTGGTCCTGGCTGTTGCTCTCTTCTTACTG
NM_152932.2         ATGTCATTCCGTAAAGTAAACATCATCATCTTGGTCCTGGCTGTTGCTCTCTTCTTACTG
NM_001278281.1      ATGTCATTCCGTAAAGTAAACATCATCATCTTGGTCCTGGCTGTTGCTCTCTTCTTACTG
NM_001010983.2      ATGTCATTCCGTAAAGTAAACATCATCATCTTGGTCCTGGCTGTTGCTCTCTTCTTACTG
NM_018446.3         ATGTCATTCCGTAAAGTAAACATCATCATCTTGGTCCTGGCTGTTGCTCTCTTCTTACTG
                                                                                

HsCD00358283        ------------------------------------------------------------
NM_001278280.1      GTTTTGCACCATAACTTCCTCAGCTTGAGCAGTTTGTTAAGGAATGAGGTTACAGATTCA
NM_152932.2         GTTTTGCACCATAACTTCCTCAGCTTGAGCAGTTTGTTAAGGAATGAGGTTACAGATTCA
NM_001278281.1      GTTTTGCACCATAACTTCCTCAGCTTGAGCAGTTTGTTAAGGAATGAGGTTACAGATTCA
NM_001010983.2      GTTTTGCACCATAACTTCCTCAGCTTGAGCAGTTTGTTAAGGAATGAGGTTACAGATTCA
NM_018446.3         GTTTTGCACCATAACTTCCTCAGCTTGAGCAGTTTGTTAAGGAATGAGGTTACAGATTCA
                                                                                

HsCD00358283        ---------------------------------CCAAATGCTCTCCGACATGCAGTAGAT
NM_001278280.1      GGAATTGTAGGGCCTCAACCTATAGACTTTGTCCCAAATGCTCTCCGACATGCAGTAGAT
NM_152932.2         GGAATTGTAGGGCCTCAACCTATAGACTTTGTCCCAAATGCTCTCCGACATGCAGTAGAT
NM_001278281.1      GGAATTGTAGGGCCTCAACCTATAGACTTTGTCCCAAATGCTCTCCGACATGCAGTAGAT
NM_001010983.2      GGAATTGTAGGGCCTCAACCTATAGACTTTGTCCCAAATGCTCTCCGACATGCAGTAGAT
NM_018446.3         GGAATTGTAGGGCCTCAACCTATAGACTTTGTCCCAAATGCTCTCCGACATGCAGTAGAT
                                                     ***************************

HsCD00358283        GGGAGACAAGAGGAGATTCCTGTGGTCATCGCTGCATCTGAAGACAGGCTTGGGGGGGCC
NM_001278280.1      GGGAGACAAGAGGAGATTCCTGTGGTCATCGCTGCATCTGAAGACAGGCTTGGGGGGGCC
NM_152932.2         GGGAGACAAGAGGAGATTCCTGTGGTCATCGCTGCATCTGAAGACAGGCTTGGGGGGGCC
NM_001278281.1      GGGAGACAAGAGGAGATTCCTGTGGTCATCGCTGCATCTGAAGACAGGCTTGGGGGGGCC
NM_001010983.2      GGGAGACAAGAGGAGATTCCTGTGGTCATCGCTGCATCTGAAGACAGGCTTGGGGGGGCC
NM_018446.3         GGGAGACAAGAGGAGATTCCTGTGGTCATCGCTGCATCTGAAGACAGGCTTGGGGGGGCC
                    ************************************************************

HsCD00358283        ATTGCAGCTATAAACAGCATTCAGCACAACACTCGCTCCAATGTGATTTTCTACATTGTT
NM_001278280.1      ATTGCAGCTATAAACAGCATTCAGCACAACACTCGCTCCAATGTGATTTTCTACATTGTT
NM_152932.2         ATTGCAGCTATAAACAGCATTCAGCACAACACTCGCTCCAATGTGATTTTCTACATTGTT
NM_001278281.1      ATTGCAGCTATAAACAGCATTCAGCACAACACTCGCTCCAATGTGATTTTCTACATTGTT
NM_001010983.2      ATTGCAGCTATAAACAGCATTCAGCACAACACTCGCTCCAATGTGATTTTCTACATTGTT
NM_018446.3         ATTGCAGCTATAAACAGCATTCAGCACAACACTCGCTCCAATGTGATTTTCTACATTGTT
                    ************************************************************

HsCD00358283        ACTCTCAACAATACAGCAGACCATCTCCGGTCCTGGCTCAACAGTGATTCCCTGAAAAGC
NM_001278280.1      ACTCTCAACAATACAGCAGACCATCTCCGGTCCTGGCTCAACAGTGATTCCCTGAAAAGC
NM_152932.2         ACTCTCAACAATACAGCAGACCATCTCCGGTCCTGGCTCAACAGTGATTCCCTGAAAAGC
NM_001278281.1      ACTCTCAACAATACAGCAGACCATCTCCGGTCCTGGCTCAACAGTGATTCCCTGAAAAGC
NM_001010983.2      ACTCTCAACAATACAGCAGACCATCTCCGGTCCTGGCTCAACAGTGATTCCCTGAAAAGC
NM_018446.3         ACTCTCAACAATACAGCAGACCATCTCCGGTCCTGGCTCAACAGTGATTCCCTGAAAAGC
                    ************************************************************

HsCD00358283        ATCAGATACAAAATTGTCAATTTTGACCCTAAACTTTTGGAAGGAAAAGTAAAGGAGGAT
NM_001278280.1      ATCAGATACAAAATTGTCAATTTTGACCCTAAACTTTTGGAAGGAAAAGTAAAGGAGGAT
NM_152932.2         ATCAGATACAAAATTGTCAATTTTGACCCTAAACTTTTGGAAGGAAAAGTAAAGGAGGAT
NM_001278281.1      ATCAGATACAAAATTGTCAATTTTGACCCTAAACTTTTGGAAGGAAAAGTAAAGGAGGAT
NM_001010983.2      ATCAGATACAAAATTGTCAATTTTGACCCTAAACTTTTGGAAGGAAAAGTAAAGGAGGAT
NM_018446.3         ATCAGATACAAAATTGTCAATTTTGACCCTAAACTTTTGGAAGGAAAAGTAAAGGAGGAT
                    ************************************************************

HsCD00358283        CCTGACCAGGGGGAATCCATGAAACCTTTAACCTTTGCAAGGTTCTACTTGCCAATTCTG
NM_001278280.1      CCTGACCAGGGGGAATCCATGAAACCTTTAACCTTTGCAAGGTTCTACTTGCCAATTCTG
NM_152932.2         CCTGACCAGGGGGAATCCATGAAACCTTTAACCTTTGCAAGGTTCTACTTGCCAATTCTG
NM_001278281.1      CCTGACCAGGGGGAATCCATGAAACCTTTAACCTTTGCAAGGTTCTACTTGCCAATTCTG
NM_001010983.2      CCTGACCAGGGGGAATCCATGAAACCTTTAACCTTTGCAAGGTTCTACTTGCCAATTCTG
NM_018446.3         CCTGACCAGGGGGAATCCATGAAACCTTTAACCTTTGCAAGGTTCTACTTGCCAATTCTG
                    ************************************************************

HsCD00358283        GTTCCCAGCGCAAAGAAGGCCATATACATGGATGATGATGTAATTGTGCAAGGTGATATT
NM_001278280.1      GTTCCCAGCGCAAAGAAGGCCATATACATGGATGATGATGTAATTGTGCAAGGTGATATT
NM_152932.2         GTTCCCAGCGCAAAGAAGGCCATATACATGGATGATGATGTAATTGTGCAAGGTGATATT
NM_001278281.1      GTTCCCAGCGCAAAGAAGGCCATATACATGGATGATGATGTAATTGTGCAAGGTGATATT
NM_001010983.2      GTTCCCAGCGCAAAGAAGGCCATATACATGGATGATGATGTAATTGTGCAAGGTGATATT
NM_018446.3         GTTCCCAGCGCAAAGAAGGCCATATACATGGATGATGATGTAATTGTGCAAGGTGATATT
                    ************************************************************

HsCD00358283        CTTGCCCTTTACAATACAGCACTGAAGCCAGGACATGCAGCTGCATTTTCAGAAGATTGT
NM_001278280.1      CTTGCCCTTTACAATACAGCACTGAAGCCAGGACATGCAGCTGCATTTTCAGAAGATTGT
NM_152932.2         CTTGCCCTTTACAATACAGCACTGAAGCCAGGACATGCAGCTGCATTTTCAGAAGATTGT
NM_001278281.1      CTTGCCCTTTACAATACAGCACTGAAGCCAGGACATGCAGCTGCATTTTCAGAAGATTGT
NM_001010983.2      CTTGCCCTTTACAATACAGCACTGAAGCCAGGACATGCAGCTGCATTTTCAGAAGATTGT
NM_018446.3         CTTGCCCTTTACAATACAGCACTGAAGCCAGGACATGCAGCTGCATTTTCAGAAGATTGT
                    ************************************************************

HsCD00358283        GATTCAGCCTCTACTAAAGTTGTCATCCGTGGAGCAGGAAACCAGTACAATTACATTGGC
NM_001278280.1      GATTCAGCCTCTACTAAAGTTGTCATCCGTGGAGCAGGAAACCAGTACAATTACATTGGC
NM_152932.2         GATTCAGCCTCTACTAAAGTTGTCATCCGTGGAGCAGGAAACCAGTACAATTACATTGGC
NM_001278281.1      GATTCAGCCTCTACTAAAGTTGTCATCCGTGGAGCAGGAAACCAGTACAATTACATTGGC
NM_001010983.2      GATTCAGCCTCTACTAAAGTTGTCATCCGTGGAGCAGGAAACCAGTACAATTACATTGGC
NM_018446.3         GATTCAGCCTCTACTAAAGTTGTCATCCGTGGAGCAGGAAACCAGTACAATTACATTGGC
                    ************************************************************

HsCD00358283        TATCTTGACTATAAAAAGGAAAGAATTCGTAAGCTTTCCATGAAAGCCAGCACTTGCTCA
NM_001278280.1      TATCTTGACTATAAAAAGGAAAGAATTCGTAAGCTTTCCATGAAAGCCAGCACTTGCTCA
NM_152932.2         TATCTTGACTATAAAAAGGAAAGAATTCGTAAGCTTTCCATGAAAGCCAGCACTTGCTCA
NM_001278281.1      TATCTTGACTATAAAAAGGAAAGAATTCGTAAGCTTTCCATGAAAGCCAGCACTTGCTCA
NM_001010983.2      TATCTTGACTATAAAAAGGAAAGAATTCGTAAGCTTTCCATGAAAGCCAGCACTTGCTCA
NM_018446.3         TATCTTGACTATAAAAAGGAAAGAATTCGTAAGCTTTCCATGAAAGCCAGCACTTGCTCA
                    ************************************************************

HsCD00358283        TTTAATCCTGGAGTTTTTGTTGCAAACCTGACGGAATGGAAACGACAGAATATAACTAAC
NM_001278280.1      TTTAATCCTGGAGTTTTTGTTGCAAACCTGACGGAATGGAAACGACAGAATATAACTAAC
NM_152932.2         TTTAATCCTGGAGTTTTTGTTGCAAACCTGACGGAATGGAAACGACAGAATATAACTAAC
NM_001278281.1      TTTAATCCTGGAGTTTTTGTTGCAAACCTGACGGAATGGAAACGACAGAATATAACTAAC
NM_001010983.2      TTTAATCCTGGAGTTTTTGTTGCAAACCTGACGGAATGGAAACGACAGAATATAACTAAC
NM_018446.3         TTTAATCCTGGAGTTTTTGTTGCAAACCTGACGGAATGGAAACGACAGAATATAACTAAC
                    ************************************************************

HsCD00358283        CAACTGGAAAAATGGATGAAACTCAATGTAGAAGAGGGACTGTATAGCAGAACCCTGGCT
NM_001278280.1      CAACTGGAAAAATGGATGAAACTCAATGTAGAAGAGGGACTGTATAGCAGAACCCTGGCT
NM_152932.2         CAACTGGAAAAATGGATGAAACTCAATGTAGAAGAGGGACTGTATAGCAGAACCCTGGCT
NM_001278281.1      CAACTGGAAAAATGGATGAAACTCAATGTAGAAGAGGGACTGTATAGCAGAACCCTGGCT
NM_001010983.2      CAACTGGAAAAATGGATGAAACTCAATGTAGAAGAGGGACTGTATAGCAGAACCCTGGCT
NM_018446.3         CAACTGGAAAAATGGATGAAACTCAATGTAGAAGAGGGACTGTATAGCAGAACCCTGGCT
                    ************************************************************

HsCD00358283        GGTAGCATCACAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACCATCGAT
NM_001278280.1      GGTAGCATCACAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACCATCGAT
NM_152932.2         GGTAGCATCACAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACCATCGAT
NM_001278281.1      GGTAGCATCACAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACCATCGAT
NM_001010983.2      GGTAGCATCACAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACCATCGAT
NM_018446.3         GGTAGCATCACAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACCATCGAT
                    ************************************************************

HsCD00358283        CCTATGTGGAATGTCCGCCACCTTGGTTCCAGTGCTGGAAAACGATATTCACCTCAGTTT
NM_001278280.1      CCTATGTGGAATGTCCGCCACCTTGGTTCCAGTGCTGGAAAACGATATTCACCTCAGTTT
NM_152932.2         CCTATGTGGAATGTCCGCCACCTTGGTTCCAGTGCTGGAAAACGATATTCACCTCAGTTT
NM_001278281.1      CCTATGTGGAATGTCCGCCACCTTGGTTCCAGTGCTGGAAAACGATATTCACCTCAGTTT
NM_001010983.2      CCTATGTGGAATGTCCGCCACCTTGGTTCCAGTGCTGGAAAACGATATTCACCTCAGTTT
NM_018446.3         CCTATGTGGAATGTCCGCCACCTTGGTTCCAGTGCTGGAAAACGATATTCACCTCAGTTT
                    ************************************************************

HsCD00358283        GTAAAGGCTGCCAAGTTACTCCATTGGAATGGACATTTGAAGCCATGGGGAAGGACTGCT
NM_001278280.1      GTAAAGGCTGCCAAGTTACTCCATTGGAATGGACATTTGAAGCCATGGGGAAGGACTGCT
NM_152932.2         GTAAAGGCTGCCAAGTTACTCCATTGGAATGGACATTTGAAGCCATGGGGAAGGACTGCT
NM_001278281.1      GTAAAGGCTGCCAAGTTACTCCATTGGAATGGACATTTGAAGCCATGGGGAAGGACTGCT
NM_001010983.2      GTAAAGGCTGCCAAGTTACTCCATTGGAATGGACATTTGAAGCCATGGGGAAGGACTGCT
NM_018446.3         GTAAAGGCTGCCAAGTTACTCCATTGGAATGGACATTTGAAGCCATGGGGAAGGACTGCT
                    ************************************************************

HsCD00358283        TCATATACTGATGTTTGGGAAAAATGGTATATTCCAGACCCAACAGGCAAATTCAACCTA
NM_001278280.1      TCATATACTGATGTTTGGGAAAAATGGTATATTCCAGACCCAACAGGCAAATTCAACCTA
NM_152932.2         TCATATACTGATGTTTGGGAAAAATGGTATATTCCAGACCCAACAGGCAAATTCAACCTA
NM_001278281.1      TCATATACTGATGTTTGGGAAAAATGGTATATTCCAGACCCAACAGGCAAATTCAACCTA
NM_001010983.2      TCATATACTGATGTTTGGGAAAAATGGTATATTCCAGACCCAACAGGCAAATTCAACCTA
NM_018446.3         TCATATACTGATGTTTGGGAAAAATGGTATATTCCAGACCCAACAGGCAAATTCAACCTA
                    ************************************************************

HsCD00358283        ATCCGAAGATATACCGAGATCTCAAACATAAAGTAG
NM_001278280.1      ATCCGAAGATATACCGAGATCTCAAACATAAAGTGA
NM_152932.2         ATCCGAAGATATACCGAGATCTCAAACATAAAGTGA
NM_001278281.1      ATCCGAAGATATACCGAGATCTCAAACATAAAGTGA
NM_001010983.2      ATCCGAAGATATACCGAGATCTCAAACATAAAGTGA
NM_018446.3         ATCCGAAGATATACCGAGATCTCAAACATAAAGTGA
                    **********************************..

Protein

UniProt : Q68CQ7
HPRD : 12423

Clone

Cloning Information : 560469

Organism


TAX_ID : 9606
Species Specific ID: 55830

Genome

Chromosome : 3
Map Location : 3p21.1
Ensembl : ENSG00000016864

Reagents

Labome : GLT8D1-antibody