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TREH (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TREH
Gene Name:  trehalase (brush-border membrane glycoprotein)
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358284

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1590nts         Open reading frame : 1 to 1590
Reference Sequence Alignment

CTACCCCCACCCTGTGAGAGTGAGATTTACTGCCACGGGGAGCTCCTAAACCAAGTTCAA
ATGGCCAAGCTCTACCAGGATGACAAGCAGTTTGTGGACATGCCACTGTCTATAGCTCCA
GAACAAGTCCTGCAGACCTTCACTGAGCTGTCCAGGGACCACAATCACAGCATCCCCAGG
GAGCAGCTGCAGGCGTTTGTCCACGAACACTTCCAGGCCAAGGGGCAGGAGCTGCAGCCC
TGGACCCCTGCAGACTGGAAAGACAGCCCCCAGTTCCTGCAGAAGATTTCAGATGCCAAA
CTGCGTGCCTGGGCAGGGCAGCTGCATCAGCTCTGGAAGAAGCTGGGGAAGAAGATGAAG
CCAGAGGTTCTCAGCCACCCTGAGCGGTTCTCTCTCATCTACTCAGAACATCCCTTCATT
GTGCCTGGCGGTCGCTTTGTTGAGTTCTACTACTGCTATGGGCATGTCCCCAATGGTGGG
CGCGTGTACTACCTGCAGCGGAGCCAGCCCCCACTCTTGACCCTCATGATGGATTGCTAC
TTGACTCACACCAATGACACCGCCTTTCTACAGGAAAACATTGAAACACTAGCCTTGGAA
TTGGACTTTTGGACCAAGAACAGGACTGTCTCTGTGAGCTTGGAGGGAAAGAACTACCTC
CTGAATCGCTATTATGTCCCTTATGGGGGACCCAGGCCTGAGTCCTACAGCAAAGATGTG
GAGTTGGCTGACACCTTGCCAGAAGGAGACCGGGAGGCTCTGTGGGCTGAGCTCAAGGCT
GGGGCTGAGTCTGGCTGGGACTTCTCTTCACGCTGGCTCATTGGAGGCCCAAACCCCAAC
TCGCTTAGCGGCATCCGAACAAGCAAACTGGTGCCTGTTGACCTGAATGCCTTCCTATGC
CAAGCAGAGGAGCTGATGAGCAACTTCTATTCCAGGCTGGGGAACGACTCCCAGGCCACG
AAGTACAGAATCCTGCGGTCGCAGCGCTTGGCCGCCCTGAACACAGTCCTGTGGGATGAG
CAGACCGGAGCCTGGTTCGATTACGACCTTGAGAAGAAGAAGAAAAACCGGGAGTTTTAC
CCATCCAACCTCACTCCACTCTGGGCCGGGTGTTTCTCTGACCCTGGCGTGGCGGACAAG
GCTCTGAAATACCTGGAGGACAACCGGATCCTGACTTACCAGTATGGGATCCCGACCTCT
CTCCAGAAGACAGGCCAGCAGTGGGATTTCCCCAATGCCTGGGCCCCCCTGCAGGACCTG
GTCATCAGAGGCCTGGCCAAGGCACCTTTACGTCGGGCCCAGGAAGTGGCTTTCCAGCTG
GCTCAGAATTGGATCCGAACCAATTTTGATGTCTACTCGCAGAAGTCAGCCATGTATGAG
AAGTATGACGTCAGCAACGGTGGACAGCCCGGTGGGGGAGGAGAATATGAAGTTCAGGAG
GGATTTGGCTGGACGAATGGCGTGGTCCTGATGCTGCTGGACCGCTATGGTGACCGGCTG
ACCTCAGGGGCCAAGCTGGCTTTCCTGGAGCCCCACTGCCTGGCGGCCACCCTTCTGCCC
AGCCTCCTGCTCAGCCTCCTGCCATGGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,69

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TREH
Symbol Nomenclature : TREH
SYNONYM : TRE; TREA
Designation : alpha,alpha-trehalose glucohydrolase|trehalase
Full Nomenclature : trehalase (brush-border membrane glycoprotein)
GENEID : 11181
GenBank Accession : BC109206
HGNC : 12266
MIM : 275360
Vega : OTTHUMG00000166409
Target GenBank: BC109206

 

Reference Sequence Alignment

  

HsCD00358284        ------------------------------------------------------------
XM_005277678.1      ATGCCAGGGAGGACCTGGGAGCTGTGCCTGCTACTGCTGCTGGGGCTGGGACTGGGGTCC
NM_007180.2         ATGCCAGGGAGGACCTGGGAGCTGTGCCTGCTACTGCTGCTGGGGCTGGGACTGGGGTCC
                                                                                

HsCD00358284        ---------CTACCCCCACCCTGTGAGAGTGAGATTTACTGCCACGGGGAGCTCCTAAAC
XM_005277678.1      CAGGAGGCCCTACCCCCACCCTGTGAGAGTGAGATTTACTGCCACGGGGAGCTCCTAAAC
NM_007180.2         CAGGAGGCCCTACCCCCACCCTGTGAGAGTGAGATTTACTGCCACGGGGAGCTCCTAAAC
                             ***************************************************

HsCD00358284        CAAGTTCAAATGGCCAAGCTCTACCAGGATGACAAGCAGTTTGTGGACATGCCACTGTCT
XM_005277678.1      CAAGTTCAAATGGCCAAGCTCTACCAGGATGACAAGCAGTTTGTGGACATGCCACTGTCT
NM_007180.2         CAAGTTCAAATGGCCAAGCTCTACCAGGATGACAAGCAGTTTGTGGACATGCCACTGTCT
                    ************************************************************

HsCD00358284        ATAGCTCCAGAACAAGTCCTGCAGACCTTCACTGAGCTGTCCAGGGACCACAATCACAGC
XM_005277678.1      ATAGCTCCAGAACAAGTCCTGCAGACCTTCACTGAGCTGTCCAGGGACCACAATCACAGC
NM_007180.2         ATAGCTCCAGAACAAGTCCTGCAGACCTTCACTGAGCTGTCCAGGGACCACAATCACAGC
                    ************************************************************

HsCD00358284        ATCCCCAGGGAGCAGCTGCAGGCGTTTGTCCACGAACACTTCCAGGCCAAGGGGCAGGAG
XM_005277678.1      ATCCCCAGGGAGCAGCTGCAGGCGTTTGTCCACGAACACTTCCAGGCCAAGGGGCAGGAG
NM_007180.2         ATCCCCAGGGAGCAGCTGCAGGCGTTTGTCCACGAACACTTCCAGGCCAAGGGGCAGGAG
                    ************************************************************

HsCD00358284        CTGCAGCCCTGGACCCCTGCAGACTGGAAAGACAGCCCCCAGTTCCTGCAGAAGATTTCA
XM_005277678.1      CTGCAGCCCTGGACCCCTGCAGACTGGAAAGACAGCCCCCAGTTCCTGCAGAAGATTTCA
NM_007180.2         CTGCAGCCCTGGACCCCTGCAGACTGGAAAGACAGCCCCCAGTTCCTGCAGAAGATTTCA
                    ************************************************************

HsCD00358284        GATGCCAAACTGCGTGCCTGGGCAGGGCAGCTGCATCAGCTCTGGAAGAAGCTGGGGAAG
XM_005277678.1      GATGCCAAACTGCGTGCCTGGGCAGGGCAGCTGCATCAGCTCTGGAAGAAGCTGGGGAAG
NM_007180.2         GATGCCAAACTGCGTGCCTGGGCAGGGCAGCTGCATCAGCTCTGGAAGAAGCTGGGGAAG
                    ************************************************************

HsCD00358284        AAGATGAAGCCAGAGGTTCTCAGCCACCCTGAGCGGTTCTCTCTCATCTACTCAGAACAT
XM_005277678.1      AAGATGAAGCCAGAGGTTCTCAGCCACCCTGAGCGGTTCTCTCTCATCTACTCAGAACAT
NM_007180.2         AAGATGAAGCCAGAGGTTCTCAGCCACCCTGAGCGGTTCTCTCTCATCTACTCAGAACAT
                    ************************************************************

HsCD00358284        CCCTTCATTGTGCCTGGCGGTCGCTTTGTTGAGTTCTACTACTG----------------
XM_005277678.1      CCCTTCATTGTGCCTGGCGGTCGCTTTGTTGAGTTCTACTACTG----------------
NM_007180.2         CCCTTCATTGTGCCTGGCGGTCGCTTTGTTGAGTTCTACTACTGGGACTCCTACTGGGTC
                    ********************************************                

HsCD00358284        ------------------------------------------------------------
XM_005277678.1      ------------------------------------------------------------
NM_007180.2         ATGGAGGGTCTGCTCCTCTCAGAGATGGCTGAGACGGTGAAGGGCATGCTGCAGAACTTC
                                                                                

HsCD00358284        -----------------CTATGGGCATGTCCCCAATGGTGGGCGCGTGTACTACCTGCAG
XM_005277678.1      -----------------CTATGGGCATGTCCCCAATGGTGGGCGCGTGTACTACCTGCAG
NM_007180.2         TTGGACCTGGTGAAAACCTATGGGCATGTCCCCAATGGTGGGCGCGTGTACTACCTGCAG
                                     *******************************************

HsCD00358284        CGGAGCCAGCCCCCACTCTTGACCCTCATGATGGATTGCTACTTGACTCACACCAATGAC
XM_005277678.1      CGGAGCCAGCCCCCACTCTTGACCCTCATGATGGATTGCTACTTGACTCACACCAATGAC
NM_007180.2         CGGAGCCAGCCCCCACTCTTGACCCTCATGATGGATTGCTACTTGACTCACACCAATGAC
                    ************************************************************

HsCD00358284        ACCGCCTTTCTACAGGAAAACATTGAAACACTAGCCTTGGAATTGGACTTTTGGACCAAG
XM_005277678.1      ACCGCCTTTCTACAGGAAAACATTGAAACACTAGCCTTGGAATTGGACTTTTGGACCAAG
NM_007180.2         ACCGCCTTTCTACAGGAAAACATTGAAACACTAGCCTTGGAATTGGACTTTTGGACCAAG
                    ************************************************************

HsCD00358284        AACAGGACTGTCTCTGTGAGCTTGGAGGGAAAGAACTACCTCCTGAATCGCTATTATGTC
XM_005277678.1      AACAGGACTGTCTCTGTGAGCTTGGAGGGAAAGAACTACCTCCTGAATCGCTATTATGTC
NM_007180.2         AACAGGACTGTCTCTGTGAGCTTGGAGGGAAAGAACTACCTCCTGAATCGCTATTATGTC
                    ************************************************************

HsCD00358284        CCTTATGGGGGACCCAGGCCTGAGTCCTACAGCAAAGATGTGGAGTTGGCTGACACCTTG
XM_005277678.1      CCTTATGGGGGACCCAGGCCTGAGTCCTACAGCAAAGATGTGGAGTTGGCTGACACCTTG
NM_007180.2         CCTTATGGGGGACCCAGGCCTGAGTCCTACAGCAAAGATGTGGAGTTGGCTGACACCTTG
                    ************************************************************

HsCD00358284        CCAGAAGGAGACCGGGAGGCTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGG
XM_005277678.1      CCAGAAGGAGACCGGGAGGCTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGG
NM_007180.2         CCAGAAGGAGACCGGGAGGCTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGG
                    ************************************************************

HsCD00358284        GACTTCTCTTCACGCTGGCTCATTGGAGGCCCAAACCCCAACTCGCTTAGCGGCATCCGA
XM_005277678.1      GACTTCTCTTCACGCTGGCTCATTGGAGGCCCAAACCCCAACTCGCTTAGCGGCATCCGA
NM_007180.2         GACTTCTCTTCACGCTGGCTCATTGGAGGCCCAAACCCCAACTCGCTTAGCGGCATCCGA
                    ************************************************************

HsCD00358284        ACAAGCAAACTGGTGCCTGTTGACCTGAATGCCTTCCTATGCCAAGCAGAGGAGCTGATG
XM_005277678.1      ACAAGCAAACTGGTGCCTGTTGACCTGAATGCCTTCCTATGCCAAGCAGAGGAGCTGATG
NM_007180.2         ACAAGCAAACTGGTGCCTGTTGACCTGAATGCCTTCCTATGCCAAGCAGAGGAGCTGATG
                    ************************************************************

HsCD00358284        AGCAACTTCTATTCCAGGCTGGGGAACGACTCCCAGGCCACGAAGTACAGAATCCTGCGG
XM_005277678.1      AGCAACTTCTATTCCAGGCTGGGGAACGACTCCCAGGCCACGAAGTACAGAATCCTGCGG
NM_007180.2         AGCAACTTCTATTCCAGGCTGGGGAACGACTCCCAGGCCACGAAGTACAGAATCCTGCGG
                    ************************************************************

HsCD00358284        TCGCAGCGCTTGGCCGCCCTGAACACAGTCCTGTGGGATGAGCAGACCGGAGCCTGGTTC
XM_005277678.1      TCGCAGCGCTTGGCCGCCCTGAACACAGTCCTGTGGGATGAGCAGACCGGAGCCTGGTTC
NM_007180.2         TCGCAGCGCTTGGCCGCCCTGAACACAGTCCTGTGGGATGAGCAGACCGGAGCCTGGTTC
                    ************************************************************

HsCD00358284        GATTACGACCTTGAGAAGAAGAAGAAAAACCGGGAGTTTTACCCATCCAACCTCACTCCA
XM_005277678.1      GATTACGACCTTGAGAAGAAGAAGAAAAACCGGGAGTTTTACCCATCCAACCTCACTCCA
NM_007180.2         GATTACGACCTTGAGAAGAAGAAGAAAAACCGGGAGTTTTACCCATCCAACCTCACTCCA
                    ************************************************************

HsCD00358284        CTCTGGGCCGGGTGTTTCTCTGACCCTGGCGTGGCGGACAAGGCTCTGAAATACCTGGAG
XM_005277678.1      CTCTGGGCCGGGTGTTTCTCTGACCCTGGCGTGGCGGACAAGGCTCTGAAATACCTGGAG
NM_007180.2         CTCTGGGCCGGGTGTTTCTCTGACCCTGGCGTGGCGGACAAGGCTCTGAAATACCTGGAG
                    ************************************************************

HsCD00358284        GACAACCGGATCCTGACTTACCAGTATGGGATCCCGACCTCTCTCCAGAAGACAGGCCAG
XM_005277678.1      GACAACCGGATCCTGACTTACCAGTATGGGATCCCGACCTCTCTCCAGAAGACAGGCCAG
NM_007180.2         GACAACCGGATCCTGACTTACCAGTATGGGATCCCGACCTCTCTCCAGAAGACAGGCCAG
                    ************************************************************

HsCD00358284        CAGTGGGATTTCCCCAATGCCTGGGCCCCCCTGCAGGACCTGGTCATCAGAGGCCTGGCC
XM_005277678.1      CAGTGGGATTTCCCCAATGCCTGGGCCCCCCTGCAGGACCTGGTCATCAGAGGCCTGGCC
NM_007180.2         CAGTGGGATTTCCCCAATGCCTGGGCCCCCCTGCAGGACCTGGTCATCAGAGGCCTGGCC
                    ************************************************************

HsCD00358284        AAGGCACCTTTACGTCGGGCCCAGGAAGTGGCTTTCCAGCTGGCTCAGAATTGGATCCGA
XM_005277678.1      AAGGCACCTTTACGTCGGGCCCAGGAAGTGGCTTTCCAGCTGGCTCAGAATTGGATCCGA
NM_007180.2         AAGGCACCTTTACGTCGGGCCCAGGAAGTGGCTTTCCAGCTGGCTCAGAATTGGATCCGA
                    ************************************************************

HsCD00358284        ACCAATTTTGATGTCTACTCGCAGAAGTCAGCCATGTATGAGAAGTATGACGTCAGCAAC
XM_005277678.1      ACCAATTTTGATGTCTACTCGCAGAAGTCAGCCATGTATGAGAAGTATGACGTCAGCAAC
NM_007180.2         ACCAATTTTGATGTCTACTCGCAGAAGTCAGCCATGTATGAGAAGTATGACGTCAGCAAC
                    ************************************************************

HsCD00358284        GGTGGACAGCCCGGTGGGGGAGGAGAATATGAAGTTCAGGAGGGATTTGGCTGGACGAAT
XM_005277678.1      GGTGGACAGCCCGGTGGGGGAGGAGAATATGAAGTTCAGGAGGGATTTGGCTGGACGAAT
NM_007180.2         GGTGGACAGCCCGGTGGGGGAGGAGAATATGAAGTTCAGGAGGGATTTGGCTGGACGAAT
                    ************************************************************

HsCD00358284        GGCGTGGTCCTGATGCTGCTGGACCGCTATGGTGACCGGCTGACCTCAGGGGCCAAGCTG
XM_005277678.1      GGCGTGGTCCTGATGCTGCTGGACCGCTATGGTGACCGGCTGACCTCAGGGGCCAAGCTG
NM_007180.2         GGCGTGGTCCTGATGCTGCTGGACCGCTATGGTGACCGGCTGACCTCAGGGGCCAAGCTG
                    ************************************************************

HsCD00358284        GCTTTCCTGGAGCCCCACTGCCTGGCGGCCACCCTTCTGCCCAGCCTCCTGCTCAGCCTC
XM_005277678.1      GCTTTCCTGGAGCCCCACTGCCTGGCGGCCACCCTTCTGCCCAGCCTCCTGCTCAGCCTC
NM_007180.2         GCTTTCCTGGAGCCCCACTGCCTGGCGGCCACCCTTCTGCCCAGCCTCCTGCTCAGCCTC
                    ************************************************************

HsCD00358284        CTGCCATGGTAG
XM_005277678.1      CTGCCATGGTGA
NM_007180.2         CTGCCATGGTGA
                    **********..

Pathways

Reactome : Digestion of dietary carbohydrate
Reactome : Disease
Reactome : Glycogen storage diseases
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Myoclonic epilepsy of Lafora

Protein

UniProt : O43280
HPRD : 02038

Clone

Cloning Information : 560472

Organism


TAX_ID : 9606
Species Specific ID: 11181

Genome

Chromosome : 11
Map Location : 11q23.3
Ensembl : ENSG00000118094

Reagents

Labome : trehalase-antibody