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FUT4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FUT4
Gene Name:  fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358289

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1056nts         Open reading frame : 1 to 1056
Reference Sequence Alignment

TGGGCGTCGCCAACCCCGTCGCGACCGGTGGGCGTGCTGCTGTGGTGGGAGCCCTTCGGG
GGGCGCGATAGCGCCCCGAGGCCGCCCCCTGACTGCCGGCTGCGCTTCAACATCAGCGGC
TGCCGCCTGCTCACCGACCGCGCGTCCTACGGAGAGGCTCAGGCCGTGCTTTTCCACCAC
CGCGACCTCGTGAAGGGGCCCCCCGACTGGCCCCCGCCCTGGGGCATCCAGGCGCACACT
GCCGAGGAGGTGGATCTGCGCGTGTTGGACTACGAGGAGGCAGCGGCGGCGGCAGAAGCC
CTGGCGACCTCCAGCCCCAGGCCCCCGGGCCAGCGCTGGGTTTGGATGAACTTCGAGTCG
CCCTCGCACTCCCCGGGGCTGCGAAGCCTGGCAAGTAACCTCTTCAACTGGACGCTCTCC
TACCGGGCGGACTCGGACGTCTTTGTGCCTTATGGCTACCTCTACCCCAGAAGCCACCCC
GGCGACCCGCCCTCAGGCCTGGCCCCGCCACTGTCCAGGAAACAGGGGCTGGTGGCATGG
GTGGTGAGCCACTGGGACGAGCGCCAGGCCCGGGTCCGCTACTACCACCAACTGAGCCAA
CATGTGACCGTGGACGTGTTCGGCCGGGGCGGGCCGGGGCAGCCGGTGCCCGAAATTGGG
CTCCTGCACACAGTGGCCCGCTACAAGTTCTACCTGGCTTTCGAGAACTCGCAGCACCTG
GATTATATCACCGAGAAGCTCTGGCGCAACGCGTTGCTCGCTGGGGCGGTGCCGGTGGTG
CTGGGCCCAGACCGTGCCAACTACGAGCGCTTTGTGCCCCGCGGCGCCTTCATCCACGTG
GACGACTTCCCAAGTGCCTCCTCCCTGGCCTCGTACCTGCTTTTCCTCGACCGCAACCCC
GCGGTCTATCGCCGCTACTTCCACTGGCGCCGGAGCTACGCTGTCCACATCACCTCCTTC
TGGGACGAGCCTTGGTGCCGGGTGTGCCAGGCTGTACAGAGGGCTGGGGACCGGCCCAAG
AGCATACGGAACTTGGCCAGCTGGTTCGAGCGGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FUT4
Symbol Nomenclature : FUT4
SYNONYM : CD15; ELFT; FCT3A; FUC-TIV; FUTIV; LeX; SSEA-1
Designation : ELAM ligand fucosyltransferase|ELAM-1 ligand fucosyltransferase|Lewis X|alpha-(1,3)-fucosyltransferase 4|fucT-IV|fucosyltransferase IV|galactoside 3-L-fucosyltransferase|stage-specific embryonic antigen 1
Full Nomenclature : fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
GENEID : 2526
GenBank Accession : BC136374
HGNC : 4015
MIM : 104230
Vega : OTTHUMG00000167795
Target GenBank: BC136374

 

Reference Sequence Alignment

  

HsCD00358289      ------------------------------------------------------------
NM_002033.3       ATGAGGCGCTTGTGGGGCGCGGCCCGGAAGCCCTCGGGCGCGGGCTGGGAGAAGGAGTGG
                                                                              

HsCD00358289      ------------------------------------------------------------
NM_002033.3       GCGGAGGCGCCGCAGGAGGCTCCCGGGGCCTGGTCGGGCCGGCTGGGCCCCGGGCGCAGT
                                                                              

HsCD00358289      ------------------------------------------------------------
NM_002033.3       GGAAGAAAGGGACGGGCGGTGCCCGGTTGGGCGTCCTGGCCAGCTCACCTTGCCCTGGCG
                                                                              

HsCD00358289      ------------------------------------------------------------
NM_002033.3       GCTCGCCCCGCCCGGCACTTGGGAGGAGCAGGGCAGGGCCCGCGGCCTTTGCATTCTGGG
                                                                              

HsCD00358289      ------------------------------------------------------------
NM_002033.3       ACCGCCCCCTTCCATTCCCGGGCCAGCGGCGAGCGGCAGCGACGGCTGGAGCCGCAGCTA
                                                                              

HsCD00358289      ------------------------------------------------------------
NM_002033.3       CAGCATGAGAGCCGGTGCCGCTCCTCCACGCCTGCGGACGCGTGGCGAGCGGAGGCAGCG
                                                                              

HsCD00358289      ------------------------------------------------------------
NM_002033.3       CTGCCTGTTCGCGCCATGGGGGCACCGTGGGGCTCGCCGACGGCGGCGGCGGGCGGGCGG
                                                                              

HsCD00358289      ------------------------------------------------------------
NM_002033.3       CGCGGGTGGCGCCGAGGCCGGGGGCTGCCATGGACCGTCTGTGTGCTGGCGGCCGCCGGC
                                                                              

HsCD00358289      ---------------------------------------------------------TGG
NM_002033.3       TTGACGTGTACGGCGCTGATCACCTACGCTTGCTGGGGGCAGCTGCCGCCGCTGCCCTGG
                                                                           ***

HsCD00358289      GCGTCGCCAACCCCGTCGCGACCGGTGGGCGTGCTGCTGTGGTGGGAGCCCTTCGGGGGG
NM_002033.3       GCGTCGCCAACCCCGTCGCGACCGGTGGGCGTGCTGCTGTGGTGGGAGCCCTTCGGGGGG
                  ************************************************************

HsCD00358289      CGCGATAGCGCCCCGAGGCCGCCCCCTGACTGCCGGCTGCGCTTCAACATCAGCGGCTGC
NM_002033.3       CGCGATAGCGCCCCGAGGCCGCCCCCTGACTGCCGGCTGCGCTTCAACATCAGCGGCTGC
                  ************************************************************

HsCD00358289      CGCCTGCTCACCGACCGCGCGTCCTACGGAGAGGCTCAGGCCGTGCTTTTCCACCACCGC
NM_002033.3       CGCCTGCTCACCGACCGCGCGTCCTACGGAGAGGCTCAGGCCGTGCTTTTCCACCACCGC
                  ************************************************************

HsCD00358289      GACCTCGTGAAGGGGCCCCCCGACTGGCCCCCGCCCTGGGGCATCCAGGCGCACACTGCC
NM_002033.3       GACCTCGTGAAGGGGCCCCCCGACTGGCCCCCGCCCTGGGGCATCCAGGCGCACACTGCC
                  ************************************************************

HsCD00358289      GAGGAGGTGGATCTGCGCGTGTTGGACTACGAGGAGGCAGCGGCGGCGGCAGAAGCCCTG
NM_002033.3       GAGGAGGTGGATCTGCGCGTGTTGGACTACGAGGAGGCAGCGGCGGCGGCAGAAGCCCTG
                  ************************************************************

HsCD00358289      GCGACCTCCAGCCCCAGGCCCCCGGGCCAGCGCTGGGTTTGGATGAACTTCGAGTCGCCC
NM_002033.3       GCGACCTCCAGCCCCAGGCCCCCGGGCCAGCGCTGGGTTTGGATGAACTTCGAGTCGCCC
                  ************************************************************

HsCD00358289      TCGCACTCCCCGGGGCTGCGAAGCCTGGCAAGTAACCTCTTCAACTGGACGCTCTCCTAC
NM_002033.3       TCGCACTCCCCGGGGCTGCGAAGCCTGGCAAGTAACCTCTTCAACTGGACGCTCTCCTAC
                  ************************************************************

HsCD00358289      CGGGCGGACTCGGACGTCTTTGTGCCTTATGGCTACCTCTACCCCAGAAGCCACCCCGGC
NM_002033.3       CGGGCGGACTCGGACGTCTTTGTGCCTTATGGCTACCTCTACCCCAGAAGCCACCCCGGC
                  ************************************************************

HsCD00358289      GACCCGCCCTCAGGCCTGGCCCCGCCACTGTCCAGGAAACAGGGGCTGGTGGCATGGGTG
NM_002033.3       GACCCGCCCTCAGGCCTGGCCCCGCCACTGTCCAGGAAACAGGGGCTGGTGGCATGGGTG
                  ************************************************************

HsCD00358289      GTGAGCCACTGGGACGAGCGCCAGGCCCGGGTCCGCTACTACCACCAACTGAGCCAACAT
NM_002033.3       GTGAGCCACTGGGACGAGCGCCAGGCCCGGGTCCGCTACTACCACCAACTGAGCCAACAT
                  ************************************************************

HsCD00358289      GTGACCGTGGACGTGTTCGGCCGGGGCGGGCCGGGGCAGCCGGTGCCCGAAATTGGGCTC
NM_002033.3       GTGACCGTGGACGTGTTCGGCCGGGGCGGGCCGGGGCAGCCGGTGCCCGAAATTGGGCTC
                  ************************************************************

HsCD00358289      CTGCACACAGTGGCCCGCTACAAGTTCTACCTGGCTTTCGAGAACTCGCAGCACCTGGAT
NM_002033.3       CTGCACACAGTGGCCCGCTACAAGTTCTACCTGGCTTTCGAGAACTCGCAGCACCTGGAT
                  ************************************************************

HsCD00358289      TATATCACCGAGAAGCTCTGGCGCAACGCGTTGCTCGCTGGGGCGGTGCCGGTGGTGCTG
NM_002033.3       TATATCACCGAGAAGCTCTGGCGCAACGCGTTGCTCGCTGGGGCGGTGCCGGTGGTGCTG
                  ************************************************************

HsCD00358289      GGCCCAGACCGTGCCAACTACGAGCGCTTTGTGCCCCGCGGCGCCTTCATCCACGTGGAC
NM_002033.3       GGCCCAGACCGTGCCAACTACGAGCGCTTTGTGCCCCGCGGCGCCTTCATCCACGTGGAC
                  ************************************************************

HsCD00358289      GACTTCCCAAGTGCCTCCTCCCTGGCCTCGTACCTGCTTTTCCTCGACCGCAACCCCGCG
NM_002033.3       GACTTCCCAAGTGCCTCCTCCCTGGCCTCGTACCTGCTTTTCCTCGACCGCAACCCCGCG
                  ************************************************************

HsCD00358289      GTCTATCGCCGCTACTTCCACTGGCGCCGGAGCTACGCTGTCCACATCACCTCCTTCTGG
NM_002033.3       GTCTATCGCCGCTACTTCCACTGGCGCCGGAGCTACGCTGTCCACATCACCTCCTTCTGG
                  ************************************************************

HsCD00358289      GACGAGCCTTGGTGCCGGGTGTGCCAGGCTGTACAGAGGGCTGGGGACCGGCCCAAGAGC
NM_002033.3       GACGAGCCTTGGTGCCGGGTGTGCCAGGCTGTACAGAGGGCTGGGGACCGGCCCAAGAGC
                  ************************************************************

HsCD00358289      ATACGGAACTTGGCCAGCTGGTTCGAGCGGTAG
NM_002033.3       ATACGGAACTTGGCCAGCTGGTTCGAGCGGTGA
                  *******************************..

Protein

UniProt : P22083
HPRD : 00083

Clone

Cloning Information : 560506

Organism


TAX_ID : 9606
Species Specific ID: 2526

Genome

Chromosome : 11
Map Location : 11q21
Ensembl : ENSG00000196371

Reagents

Labome : CD15-antibody