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HYAL4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HYAL4
Gene Name:  hyaluronoglucosaminidase 4
Sequence             
Original Clone ID: None
Keyword: partial cds (N and C terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358291

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1275nts         Open reading frame : 1 to 1275
Reference Sequence Alignment

AAGTCTATCTCTTGTCTAAAACCTGCTCGACTTCCAATTTATCAAAGGAAACCTTTTATA
GCTGCTTGGAATGCTCCAACAGATCAGTGTTTGATAAAATATAATTTAAGACTAAATTTG
AAAATGTTTCCTGTGATTGGAAGCCCACTGGCCAAGGCCAGGGGGCAAAATGTCACTATA
TTTTATGTCAACAGATTGGGATACTATCCGTGGTATACATCACAAGGGGTCCCCATTAAT
GGAGGTCTCCCACAGAACATAAGTTTACAAGTACATCTGGAAAAAGCTGACCAAGATATT
AATTATTACATCCCTGCTGAAGATTTCAGTGGACTTGCTGTTATAGATTGGGAATATTGG
CGACCACAGTGGGCCCGGAACTGGAACTCAAAAGATGTTTACAGACAGAAGTCAAGAAAG
CTTATTTCCGATATGGGAAAGAATGTATCAGCTACCGATATTGAATATTTAGCCAAAGTG
ACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAGGAAACCATCAAATTGGGAATTAAGAGC
CGACCCAAAGGCCTTTGGGGTTATTATTTATATCCTGATTGCCACAATTATAACGTTTAT
GCCCCAAACTACTCTGGGTCATGCCCAGAAGACGAAGTCTTGAGGAACAATGAGCTCTCT
TGGCTCTGGAACAGCAGTGCTGCTTTATATCCTTCTATCGGTGTCTGGAAATCCCTTGGA
GACAGTGAAAACATTTTGCGCTTCTCCAAATTTCGGGTGCATGAATCCATGAGGATCTCC
ACCATGACATCTCATGATTATGCTCTGCCTGTATTTGTCTACACAAGGCTAGGGTACAGA
GATGAACCTTTATTTTTCCTTTCTAAGCAAGATCTAGTCAGCACCATAGGAGAAAGTGCT
GCCTTGGGAGCTGCAGGCATTGTTATTTGGGGAGACATGAATTTAACTTCATCCAAGGCC
AACTGTACAAAGGTGAAGCAGTTTGTGAGTTCTGATTTAGGGAGCTACATAGCCAATGTG
ACCAGAGCTGCTGAGGTATGCAGCCTTCACCTCTGCAGGAACAATGGCAGGTGCATAAGG
AAGATGTGGAACGCGCCCAGTTACCTTCACTTGAACCCTGCAAGTTACCACATAGAGGCC
TCTGAGGACGGGGAGTTTACTGTGAAAGGAAAAGCATCTGATACAGACCTGGCAGTGATG
GCAGATACATTTTCCTGTCATTGTTATCAGGGATATGAAGGAGCTGATTGCAGAGAAATA
AAGACGGCTGATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,87; del@1363,84

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HYAL4
Symbol Nomenclature : HYAL4
SYNONYM : CSHY
Designation : chondroitin sulfate endo-beta-N-acetylgalactosaminidase|chondroitin sulfate hydrolase|hyal-4|hyaluronidase 4|hyaluronidase-4|hyaluronoglucosaminidase-4
Full Nomenclature : hyaluronoglucosaminidase 4
GENEID : 23553
GenBank Accession : BC104788
HGNC : 5323
MIM : 604510
Vega : OTTHUMG00000157349
Target GenBank: BC104788

 

Reference Sequence Alignment

  

HsCD00358291        ------------------------------------------------------------
XM_005250238.1      ATGAAAGTATTATCTGAAGGACAGTTAAAGCTTTGTGTTGTTCAACCAGTACATCTCACT
NM_012269.2         ATGAAAGTATTATCTGAAGGACAGTTAAAGCTTTGTGTTGTTCAACCAGTACATCTCACT
                                                                                

HsCD00358291        ---------------------------AAGTCTATCTCTTGTCTAAAACCTGCTCGACTT
XM_005250238.1      TCATGGCTCCTTATATTTTTTATTCTAAAGTCTATCTCTTGTCTAAAACCTGCTCGACTT
NM_012269.2         TCATGGCTCCTTATATTTTTTATTCTAAAGTCTATCTCTTGTCTAAAACCTGCTCGACTT
                                               *********************************

HsCD00358291        CCAATTTATCAAAGGAAACCTTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTG
XM_005250238.1      CCAATTTATCAAAGGAAACCTTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTG
NM_012269.2         CCAATTTATCAAAGGAAACCTTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTG
                    ************************************************************

HsCD00358291        ATAAAATATAATTTAAGACTAAATTTGAAAATGTTTCCTGTGATTGGAAGCCCACTGGCC
XM_005250238.1      ATAAAATATAATTTAAGACTAAATTTGAAAATGTTTCCTGTGATTGGAAGCCCACTGGCC
NM_012269.2         ATAAAATATAATTTAAGACTAAATTTGAAAATGTTTCCTGTGATTGGAAGCCCACTGGCC
                    ************************************************************

HsCD00358291        AAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACAGATTGGGATACTATCCGTGG
XM_005250238.1      AAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACAGATTGGGATACTATCCGTGG
NM_012269.2         AAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACAGATTGGGATACTATCCGTGG
                    ************************************************************

HsCD00358291        TATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAGTTTACAAGTA
XM_005250238.1      TATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAGTTTACAAGTA
NM_012269.2         TATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAGTTTACAAGTA
                    ************************************************************

HsCD00358291        CATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
XM_005250238.1      CATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
NM_012269.2         CATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
                    ************************************************************

HsCD00358291        CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAA
XM_005250238.1      CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAA
NM_012269.2         CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAA
                    ************************************************************

HsCD00358291        GATGTTTACAGACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCT
XM_005250238.1      GATGTTTACAGACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCT
NM_012269.2         GATGTTTACAGACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCT
                    ************************************************************

HsCD00358291        ACCGATATTGAATATTTAGCCAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAG
XM_005250238.1      ACCGATATTGAATATTTAGCCAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAG
NM_012269.2         ACCGATATTGAATATTTAGCCAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAG
                    ************************************************************

HsCD00358291        GAAACCATCAAATTGGGAATTAAGAGCCGACCCAAAGGCCTTTGGGGTTATTATTTATAT
XM_005250238.1      GAAACCATCAAATTGGGAATTAAGAGCCGACCCAAAGGCCTTTGGGGTTATTATTTATAT
NM_012269.2         GAAACCATCAAATTGGGAATTAAGAGCCGACCCAAAGGCCTTTGGGGTTATTATTTATAT
                    ************************************************************

HsCD00358291        CCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACTCTGGGTCATGCCCAGAAGAC
XM_005250238.1      CCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACTCTGGGTCATGCCCAGAAGAC
NM_012269.2         CCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACTCTGGGTCATGCCCAGAAGAC
                    ************************************************************

HsCD00358291        GAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGCTTTATATCCT
XM_005250238.1      GAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGCTTTATATCCT
NM_012269.2         GAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGCTTTATATCCT
                    ************************************************************

HsCD00358291        TCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
XM_005250238.1      TCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
NM_012269.2         TCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
                    ************************************************************

HsCD00358291        CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTA
XM_005250238.1      CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTA
NM_012269.2         CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTA
                    ************************************************************

HsCD00358291        TTTGTCTACACAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGAT
XM_005250238.1      TTTGTCTACACAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGAT
NM_012269.2         TTTGTCTACACAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGAT
                    ************************************************************

HsCD00358291        CTAGTCAGCACCATAGGAGAAAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGA
XM_005250238.1      CTAGTCAGCACCATAGGAGAAAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGA
NM_012269.2         CTAGTCAGCACCATAGGAGAAAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGA
                    ************************************************************

HsCD00358291        GACATGAATTTAACTTCATCCAAGGCCAACTGTACAAAGGTGAAGCAGTTTGTGAGTTCT
XM_005250238.1      GACATGAATTTAACTGCATCCAAGGCCAACTGTACAAAGGTGAAGCAGTTTGTGAGTTCT
NM_012269.2         GACATGAATTTAACTGCATCCAAGGCCAACTGTACAAAGGTGAAGCAGTTTGTGAGTTCT
                    *************** ********************************************

HsCD00358291        GATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTGAGGTATGCAGCCTTCACCTC
XM_005250238.1      GATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTGAGGTATGCAGCCTTCACCTC
NM_012269.2         GATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTGAGGTATGCAGCCTTCACCTC
                    ************************************************************

HsCD00358291        TGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTACCTTCACTTG
XM_005250238.1      TGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTACCTTCACTTG
NM_012269.2         TGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTACCTTCACTTG
                    ************************************************************

HsCD00358291        AACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
XM_005250238.1      AACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
NM_012269.2         AACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
                    ************************************************************

HsCD00358291        GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGA
XM_005250238.1      GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGA
NM_012269.2         GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGA
                    ************************************************************

HsCD00358291        TATGAAGGAGCTGATTGCAGAGAAATAAAGACGGCTGATTAG------------------
XM_005250238.1      TATGAAGGAGCTGATTGCAGAGAAATAAAGACGGCTGATGGCTGCTCTGGGGTTTCCCCT
NM_012269.2         TATGAAGGAGCTGATTGCAGAGAAATAAAGACGGCTGATGGCTGCTCTGGGGTTTCCCCT
                    *************************************** .                   

HsCD00358291        ------------------------------------------------------------
XM_005250238.1      TCTCCTGGTTCACTAATGACACTTTGTCTACTGCTTTTAGCAAGTTATCGAAGCATTCAG
NM_012269.2         TCTCCTGGTTCACTAATGACACTTTGTCTACTGCTTTTAGCAAGTTATCGAAGCATTCAG
                                                                                

HsCD00358291        ------
XM_005250238.1      TTGTGA
NM_012269.2         TTGTGA
                          

Protein

UniProt : Q2M3T9
UniProt : D0VXG1
HPRD : 05148

Clone

Cloning Information : 560524

Organism


TAX_ID : 9606
Species Specific ID: 23553

Genome

Chromosome : 7
Map Location : 7q31.3
Ensembl : ENSG00000106302

Reagents

Labome : HYAL4-antibody