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EDEM2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EDEM2
Gene Name:  ER degradation enhancer, mannosidase alpha-like 2
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358301

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 1674nts         Open reading frame : 1 to 1674
Reference Sequence Alignment

GCGCCAGGTCCCGACGGCTCCGCGCCAGATCCCGCCCACTACAGGGAGCGAGTCAAGGCC
ATGTTCTACCACGCCTACGACAGCTACCTGGAGAATGCCTTTCCCTTCGATGAGCTGCGA
CCTCTCACCTGTGACGGGCACGACACCTGGGGCAGTTTTTCTCTGACTCTAATTGATGCA
CTGGACACCTTGCTGATTTTGGGGAATGTCTCAGAATTCCAAAGAGTGGTTGAAGTGCTC
CAGGACAGCGTGGACTTTGATATTGATGTGAACGCCTCTGTGTTTGAAACAAACATTCGA
GTGGTAGGAGGACTCCTGTCTGCTCATCTGCTCTCCAAGAAGGCTGGGGTGGAAGTAGAG
GCTGGATGGCCCTGTTCCGGGCCTCTCCTGAGAATGGCTGAGGAGGCGGCCCGAAAACTC
CTCCCAGCCTTTCAGACCCCCACTGGCATGCCATATGGAACAGTGAACTTACTTCATGGC
GTGAACCCAGGAGAGACCCCTGTCACCTGTACGGCAGGGATTGGGACCTTCATTGTTGAA
TTTGCCACCCTGAGCAGCCTCACTGGTGACCCGGTGTTCGAAGATGTGGCCAGAGTGGCT
TTGATGCGCCTCTGGGAGAGCCGGTCAGATATCGGGCTGGTCGGCAACCACATTGATGTG
CTCACTGGCAAGTGGGTGGCCCAGGACGCAGGCATCGGGGCTGGCGTGGACTCCTACTTT
GAGTACTTGGTGAAAGGAGCCATCCTGCTTCAGGATAAGAAGCTCATGGCCATGTTCCTA
GAGTATAACAAAGCCATCCGGAACTACACCCGCTTCGATGACTGGTACCTGTGGGTTCAG
ATGTACAAGGGGACTGTGTCCATGCCAGTCTTCCAGTCCTTGGAGGCCTACTGGCCTGGT
CTTCAGAGCCTCATTGGAGACATTGACAATGCCATGAGGACCTTCCTCAACTACTACACT
GTATGGAAGCAGTTTGGGGGGCTCCCGGAATTCTACAACATTCCTCAGGGATACACAGTG
GAGAAGCGAGAGGGCTACCCACTTCGGCCAGAACTTATTGAAAGCGCAATGTACCTCTAC
CGTGCCACGGGGGATCCCACCCTCCTAGAACTCGGAAGAGATGCTGTGGAATCCATTGAA
AAAATCAGCAAGGTGGAGTGCGGATTTGCAACAATCAAAGATCTGCGAGACCACAAGCTG
GACAACCGCATGGAGTCGTTCTTCCTGGCCGAGACTGTGAAATACCTCTACCTCCTGTTT
GACCCAACCAACTTCATCCACAACAATGGGTCCACCTTCGACACGGTGATCACCCCCTAT
GGGGAGTGCATCCTGGGGGCTGGGGGGTACATCTTCAACACAGAAGCTCACCCCATCGAC
CCTGCCGCCCTGCACTGCTGCCAGAGGCTGAAGGAAGAGCAGTGGGAGGTGGAGGACTTG
ATGAGGGAATTCTACTCTCTCAAACGGAGCAGGTCGAAATTTCAGAAAAACACTGTTAGT
TCGGGGCCATGGGAACCTCCAGCAAGGCCAGGAACACTCTTCTCACCAGAAAACCATGAC
CAGGCAAGGGAGAGGAAGCCTGCCAAACAGAAGGTCCCACTTCTCAGCTGCCCCAGTCAG
CCCTTCACCTCCAAGTTGGCATTACTGGGACAGGTTTTCCTAGACTCCTCATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,63

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EDEM2
Symbol Nomenclature : EDEM2
SYNONYM : C20orf31; C20orf49; bA4204.1
Designation : ER degradation-enhancing alpha-mannosidase-like 2|ER degradation-enhancing alpha-mannosidase-like protein 2|ER degradation-enhancing-mannosidase-like protein 2
Full Nomenclature : ER degradation enhancer, mannosidase alpha-like 2
GENEID : 55741
Locus Tag : UNQ573/PRO1135
GenBank Accession : BC001371
HGNC : 15877
MIM : 610302
Vega : OTTHUMG00000032322
Target GenBank: BC001371

 

Reference Sequence Alignment

  

HsCD00358301        GCGCCAGGTCCCGACGGCTCCGCGCCAGATCCCGCCCACTACAGGGAGCGAGTCAAGGCC
NM_001145025.1      ----------ATGCCTTTCCGGCTGCTCATCCCGCTCGGCCTCCTGTGCGCGCTGCTGCC
NM_018217.2         ----------ATGCCTTTCCGGCTGCTCATCCCGCTCGGCCTCCTGTGCGCGCTGCTGCC
                              . *.*    * **  *: ******* *.  . .  *:***.*  .. ***

HsCD00358301        ATGTTCTACCACGCCTACGACAGCTACCTGGAGAATGCCTTTCCCTTCGATGAGCTGCGA
NM_001145025.1      TCAGCACCATGGTGCGCCAGGTCCCGACGGCTCCGCGCCAGATCCCGCCCACTACAG---
NM_018217.2         TCAGCACCATGGTGCGCCAGGTCCCGACGGCTCCGCGCCAGATCCCGCCCACTACAGGGA
                    : .  . .. .   * .*.. : * ..* * : .. ***: : **  * .: :.*:*   

HsCD00358301        CCTCTCACCTGTGACGGGCACG--------------------------------------
NM_001145025.1      ------------------------------------------------------------
NM_018217.2         GCGAGTCAAGGCCATGTTCTACCACGCCTACGACAGCTACCTGGAGAATGCCTTTCCCTT
                                                                                

HsCD00358301        -----------------------------------ACACCTGGGGCAGTTTTTCTCTGAC
NM_001145025.1      ------------------------------------------------TTTTTCTCTGAC
NM_018217.2         CGATGAGCTGCGACCTCTCACCTGTGACGGGCACGACACCTGGGGCAGTTTTTCTCTGAC
                                                                    ************

HsCD00358301        TCTAATTGATGCACTGGACACCTTGCTGATTTTGGGGAATGTCTCAGAATTCCAAAGAGT
NM_001145025.1      TCTAATTGATGCACTGGACACCTTGCTGATTTTGGGGAATGTCTCAGAATTCCAAAGAGT
NM_018217.2         TCTAATTGATGCACTGGACACCTTGCTGATTTTGGGGAATGTCTCAGAATTCCAAAGAGT
                    ************************************************************

HsCD00358301        GGTTGAAGTGCTCCAGGACAGCGTGGACTTTGATATTGATGTGAACGCCTCTGTGTTTGA
NM_001145025.1      GGTTGAAGTGCTCCAGGACAGCGTGGACTTTGATATTGATGTGAACGCCTCTGTGTTTGA
NM_018217.2         GGTTGAAGTGCTCCAGGACAGCGTGGACTTTGATATTGATGTGAACGCCTCTGTGTTTGA
                    ************************************************************

HsCD00358301        AACAAACATTCGAGTGGTAGGAGGACTCCTGTCTGCTCATCTGCTCTCCAAGAAGGCTGG
NM_001145025.1      AACAAACATTCGAGTGGTAGGAGGACTCCTGTCTGCTCATCTGCTCTCCAAGAAGGCTGG
NM_018217.2         AACAAACATTCGAGTGGTAGGAGGACTCCTGTCTGCTCATCTGCTCTCCAAGAAGGCTGG
                    ************************************************************

HsCD00358301        GGTGGAAGTAGAGGCTGGATGGCCCTGTTCCGGGCCTCTCCTGAGAATGGCTGAGGAGGC
NM_001145025.1      GGTGGAAGTAGAGGCTGGATGGCCCTGTTCCGGGCCTCTCCTGAGAATGGCTGAGGAGGC
NM_018217.2         GGTGGAAGTAGAGGCTGGATGGCCCTGTTCCGGGCCTCTCCTGAGAATGGCTGAGGAGGC
                    ************************************************************

HsCD00358301        GGCCCGAAAACTCCTCCCAGCCTTTCAGACCCCCACTGGCATGCCATATGGAACAGTGAA
NM_001145025.1      GGCCCGAAAACTCCTCCCAGCCTTTCAGACCCCCACTGGCATGCCATATGGAACAGTGAA
NM_018217.2         GGCCCGAAAACTCCTCCCAGCCTTTCAGACCCCCACTGGCATGCCATATGGAACAGTGAA
                    ************************************************************

HsCD00358301        CTTACTTCATGGCGTGAACCCAGGAGAGACCCCTGTCACCTGTACGGCAGGGATTGGGAC
NM_001145025.1      CTTACTTCATGGCGTGAACCCAGGAGAGACCCCTGTCACCTGTACGGCAGGGATTGGGAC
NM_018217.2         CTTACTTCATGGCGTGAACCCAGGAGAGACCCCTGTCACCTGTACGGCAGGGATTGGGAC
                    ************************************************************

HsCD00358301        CTTCATTGTTGAATTTGCCACCCTGAGCAGCCTCACTGGTGACCCGGTGTTCGAAGATGT
NM_001145025.1      CTTCATTGTTGAATTTGCCACCCTGAGCAGCCTCACTGGTGACCCGGTGTTCGAAGATGT
NM_018217.2         CTTCATTGTTGAATTTGCCACCCTGAGCAGCCTCACTGGTGACCCGGTGTTCGAAGATGT
                    ************************************************************

HsCD00358301        GGCCAGAGTGGCTTTGATGCGCCTCTGGGAGAGCCGGTCAGATATCGGGCTGGTCGGCAA
NM_001145025.1      GGCCAGAGTGGCTTTGATGCGCCTCTGGGAGAGCCGGTCAGATATCGGGCTGGTCGGCAA
NM_018217.2         GGCCAGAGTGGCTTTGATGCGCCTCTGGGAGAGCCGGTCAGATATCGGGCTGGTCGGCAA
                    ************************************************************

HsCD00358301        CCACATTGATGTGCTCACTGGCAAGTGGGTGGCCCAGGACGCAGGCATCGGGGCTGGCGT
NM_001145025.1      CCACATTGATGTGCTCACTGGCAAGTGGGTGGCCCAGGACGCAGGCATCGGGGCTGGCGT
NM_018217.2         CCACATTGATGTGCTCACTGGCAAGTGGGTGGCCCAGGACGCAGGCATCGGGGCTGGCGT
                    ************************************************************

HsCD00358301        GGACTCCTACTTTGAGTACTTGGTGAAAGGAGCCATCCTGCTTCAGGATAAGAAGCTCAT
NM_001145025.1      GGACTCCTACTTTGAGTACTTGGTGAAAGGAGCCATCCTGCTTCAGGATAAGAAGCTCAT
NM_018217.2         GGACTCCTACTTTGAGTACTTGGTGAAAGGAGCCATCCTGCTTCAGGATAAGAAGCTCAT
                    ************************************************************

HsCD00358301        GGCCATGTTCCTAGAGTATAACAAAGCCATCCGGAACTACACCCGCTTCGATGACTGGTA
NM_001145025.1      GGCCATGTTCCTAGAGTATAACAAAGCCATCCGGAACTACACCCGCTTCGATGACTGGTA
NM_018217.2         GGCCATGTTCCTAGAGTATAACAAAGCCATCCGGAACTACACCCGCTTCGATGACTGGTA
                    ************************************************************

HsCD00358301        CCTGTGGGTTCAGATGTACAAGGGGACTGTGTCCATGCCAGTCTTCCAGTCCTTGGAGGC
NM_001145025.1      CCTGTGGGTTCAGATGTACAAGGGGACTGTGTCCATGCCAGTCTTCCAGTCCTTGGAGGC
NM_018217.2         CCTGTGGGTTCAGATGTACAAGGGGACTGTGTCCATGCCAGTCTTCCAGTCCTTGGAGGC
                    ************************************************************

HsCD00358301        CTACTGGCCTGGTCTTCAGAGCCTCATTGGAGACATTGACAATGCCATGAGGACCTTCCT
NM_001145025.1      CTACTGGCCTGGTCTTCAGAGCCTCATTGGAGACATTGACAATGCCATGAGGACCTTCCT
NM_018217.2         CTACTGGCCTGGTCTTCAGAGCCTCATTGGAGACATTGACAATGCCATGAGGACCTTCCT
                    ************************************************************

HsCD00358301        CAACTACTACACTGTATGGAAGCAGTTTGGGGGGCTCCCGGAATTCTACAACATTCCTCA
NM_001145025.1      CAACTACTACACTGTATGGAAGCAGTTTGGGGGGCTCCCGGAATTCTACAACATTCCTCA
NM_018217.2         CAACTACTACACTGTATGGAAGCAGTTTGGGGGGCTCCCGGAATTCTACAACATTCCTCA
                    ************************************************************

HsCD00358301        GGGATACACAGTGGAGAAGCGAGAGGGCTACCCACTTCGGCCAGAACTTATTGAAAGCGC
NM_001145025.1      GGGATACACAGTGGAGAAGCGAGAGGGCTACCCACTTCGGCCAGAACTTATTGAAAGCGC
NM_018217.2         GGGATACACAGTGGAGAAGCGAGAGGGCTACCCACTTCGGCCAGAACTTATTGAAAGCGC
                    ************************************************************

HsCD00358301        AATGTACCTCTACCGTGCCACGGGGGATCCCACCCTCCTAGAACTCGGAAGAGATGCTGT
NM_001145025.1      AATGTACCTCTACCGTGCCACGGGGGATCCCACCCTCCTAGAACTCGGAAGAGATGCTGT
NM_018217.2         AATGTACCTCTACCGTGCCACGGGGGATCCCACCCTCCTAGAACTCGGAAGAGATGCTGT
                    ************************************************************

HsCD00358301        GGAATCCATTGAAAAAATCAGCAAGGTGGAGTGCGGATTTGCAACAATCAAAGATCTGCG
NM_001145025.1      GGAATCCATTGAAAAAATCAGCAAGGTGGAGTGCGGATTTGCAACAATCAAAGATCTGCG
NM_018217.2         GGAATCCATTGAAAAAATCAGCAAGGTGGAGTGCGGATTTGCAACAATCAAAGATCTGCG
                    ************************************************************

HsCD00358301        AGACCACAAGCTGGACAACCGCATGGAGTCGTTCTTCCTGGCCGAGACTGTGAAATACCT
NM_001145025.1      AGACCACAAGCTGGACAACCGCATGGAGTCGTTCTTCCTGGCCGAGACTGTGAAATACCT
NM_018217.2         AGACCACAAGCTGGACAACCGCATGGAGTCGTTCTTCCTGGCCGAGACTGTGAAATACCT
                    ************************************************************

HsCD00358301        CTACCTCCTGTTTGACCCAACCAACTTCATCCACAACAATGGGTCCACCTTCGACACGGT
NM_001145025.1      CTACCTCCTGTTTGACCCAACCAACTTCATCCACAACAATGGGTCCACCTTCGACGCGGT
NM_018217.2         CTACCTCCTGTTTGACCCAACCAACTTCATCCACAACAATGGGTCCACCTTCGACGCGGT
                    *******************************************************.****

HsCD00358301        GATCACCCCCTATGGGGAGTGCATCCTGGGGGCTGGGGGGTACATCTTCAACACAGAAGC
NM_001145025.1      GATCACCCCCTATGGGGAGTGCATCCTGGGGGCTGGGGGGTACATCTTCAACACAGAAGC
NM_018217.2         GATCACCCCCTATGGGGAGTGCATCCTGGGGGCTGGGGGGTACATCTTCAACACAGAAGC
                    ************************************************************

HsCD00358301        TCACCCCATCGACCCTGCCGCCCTGCACTGCTGCCAGAGGCTGAAGGAAGAGCAGTGGGA
NM_001145025.1      TCACCCCATCGACCCTGCCGCCCTGCACTGCTGCCAGAGGCTGAAGGAAGAGCAGTGGGA
NM_018217.2         TCACCCCATCGACCCTGCCGCCCTGCACTGCTGCCAGAGGCTGAAGGAAGAGCAGTGGGA
                    ************************************************************

HsCD00358301        GGTGGAGGACTTGATGAGGGAATTCTACTCTCTCAAACGGAGCAGGTCGAAATTTCAGAA
NM_001145025.1      GGTGGAGGACTTGATGAGGGAATTCTACTCTCTCAAACGGAGCAGGTCGAAATTTCAGAA
NM_018217.2         GGTGGAGGACTTGATGAGGGAATTCTACTCTCTCAAACGGAGCAGGTCGAAATTTCAGAA
                    ************************************************************

HsCD00358301        AAACACTGTTAGTTCGGGGCCATGGGAACCTCCAGCAAGGCCAGGAACACTCTTCTCACC
NM_001145025.1      AAACACTGTTAGTTCGGGGCCATGGGAACCTCCAGCAAGGCCAGGAACACTCTTCTCACC
NM_018217.2         AAACACTGTTAGTTCGGGGCCATGGGAACCTCCAGCAAGGCCAGGAACACTCTTCTCACC
                    ************************************************************

HsCD00358301        AGAAAACCATGACCAGGCAAGGGAGAGGAAGCCTGCCAAACAGAAGGTCCCACTTCTCAG
NM_001145025.1      AGAAAACCATGACCAGGCAAGGGAGAGGAAGCCTGCCAAACAGAAGGTCCCACTTCTCAG
NM_018217.2         AGAAAACCATGACCAGGCAAGGGAGAGGAAGCCTGCCAAACAGAAGGTCCCACTTCTCAG
                    ************************************************************

HsCD00358301        CTGCCCCAGTCAGCCCTTCACCTCCAAGTTGGCATTACTGGGACAGGTTTTCCTAGACTC
NM_001145025.1      CTGCCCCAGTCAGCCCTTCACCTCCAAGTTGGCATTACTGGGACAGGTTTTCCTAGACTC
NM_018217.2         CTGCCCCAGTCAGCCCTTCACCTCCAAGTTGGCATTACTGGGACAGGTTTTCCTAGACTC
                    ************************************************************

HsCD00358301        CTCATAG
NM_001145025.1      CTCATAA
NM_018217.2         CTCATAA
                    ******.

Pathways

Reactome : Asparagine N-linked glycosylation
Reactome : Calnexin/calreticulin cycle
Reactome : ER Quality Control Compartment (ERQC)
Reactome : Metabolism of proteins
Reactome : N-glycan trimming in the ER and Calnexin/Calreticulin cycle
Reactome : Post-translational protein modification

Protein

UniProt : Q9BV94
HPRD : 09841

Clone

Cloning Information : 560624

Organism


TAX_ID : 9606
Species Specific ID: 55741

Genome

Chromosome : 20
Map Location : 20q11.22
Ensembl : ENSG00000088298

Reagents

Labome : EDEM2-antibody