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C1GALT1C1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  C1GALT1C1
Gene Name:  C1GALT1-specific chaperone 1
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358302

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 873nts         Open reading frame : 1 to 873
Reference Sequence Alignment

AGGATTGGTCATGGAAATAGAATGCACCACCATGAGCATCATCACCTACAAGCTCCTAAC
AAAGAAGATATCTTGAAAATTTCAGAGGATGAGCGCATGGAGCTCAGTAAGAGCTTTCGA
GTATACTGTATTATCCTTGTAAAACCCAAAGATGTGAGTCTTTGGGCTGCAGTAAAGGAG
ACTTGGACCAAACACTGTGACAAAGCAGAGTTCTTCAGTTCTGAAAATGTTAAAGTGTTT
GAGTCAATTAATATGGACACAAATGACATGTGGTTAATGATGAGAAAAGCTTACAAATAC
GCCTTTGATAAGTATAGAGACCAATACAACTGGTTCTTCCTTGCACGCCCCACTACGTTT
GCTATCATTGAAAACCTAAAGTATTTTTTGTTAAAAAAGGATCCATCACAGCCTTTCTAT
CTAGGCCACACTATAAAATCTGGAGACCTTGAATATGTGGGTATGGAAGGAGGAATTGTC
TTAAGTGTAGAATCAATGAAAAGACTTAACAGCCTTCTCAATATCCCAGAAAAGTGTCCT
GAACAGGGAGGGATGATTTGGAAGATATCTGAAGATAAACAGCTAGCAGTTTGCCTGAAA
TATGCTGGAGTATTTGCAGAAAATGCAGAAGATGCTGATGGAAAAGATGTATTTAATACC
AAATCTGTTGGGCTTTCTATTAAAGAGGCAATGACTTATCACCCCAACCAGGTAGTAGAA
GGCTGTTGTTCAGATATGGCTGTTACTTTTAATGGACTGACTCCAAATCAGATGCATGTG
ATGATGTATGGGGTATACCGCCTTAGGGCATTTGGGCATATTTTCAATGATGCATTGGTT
TTCTTACCTCCAAATGGTTCTGACAATGACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,84

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : C1GALT1C1
Symbol Nomenclature : C1GALT1C1
SYNONYM : C1GALT2; C38H2-L1; COSMC; MST143; TNPS
Designation : C1Gal-T2|C38H2-like protein 1|beta 1,3-galactosyltransferase 2|core 1 beta1,3-galactosyltransferase 2|core 1 beta3-Gal-T2|core 1 beta3-galactosyltransferase-specific molecular chaperone
Full Nomenclature : C1GALT1-specific chaperone 1
GENEID : 29071
Locus Tag : HSPC067
GenBank Accession : BC011930
HGNC : 24338
MIM : 300611
Vega : OTTHUMG00000022305
Target GenBank: BC011930

 

Reference Sequence Alignment

  

HsCD00358302        ------------------------------------------------------------
NM_001011551.2      ATGCTTTCTGAAAGCAGCTCCTTTTTGAAGGGTGTGATGCTTGGAAGCATTTTCTGTGCT
NM_152692.4         ATGCTTTCTGAAAGCAGCTCCTTTTTGAAGGGTGTGATGCTTGGAAGCATTTTCTGTGCT
                                                                                

HsCD00358302        ------------------------AGGATTGGTCATGGAAATAGAATGCACCACCATGAG
NM_001011551.2      TTGATCACTATGCTAGGACACATTAGGATTGGTCATGGAAATAGAATGCACCACCATGAG
NM_152692.4         TTGATCACTATGCTAGGACACATTAGGATTGGTCATGGAAATAGAATGCACCACCATGAG
                                            ************************************

HsCD00358302        CATCATCACCTACAAGCTCCTAACAAAGAAGATATCTTGAAAATTTCAGAGGATGAGCGC
NM_001011551.2      CATCATCACCTACAAGCTCCTAACAAAGAAGATATCTTGAAAATTTCAGAGGATGAGCGC
NM_152692.4         CATCATCACCTACAAGCTCCTAACAAAGAAGATATCTTGAAAATTTCAGAGGATGAGCGC
                    ************************************************************

HsCD00358302        ATGGAGCTCAGTAAGAGCTTTCGAGTATACTGTATTATCCTTGTAAAACCCAAAGATGTG
NM_001011551.2      ATGGAGCTCAGTAAGAGCTTTCGAGTATACTGTATTATCCTTGTAAAACCCAAAGATGTG
NM_152692.4         ATGGAGCTCAGTAAGAGCTTTCGAGTATACTGTATTATCCTTGTAAAACCCAAAGATGTG
                    ************************************************************

HsCD00358302        AGTCTTTGGGCTGCAGTAAAGGAGACTTGGACCAAACACTGTGACAAAGCAGAGTTCTTC
NM_001011551.2      AGTCTTTGGGCTGCAGTAAAGGAGACTTGGACCAAACACTGTGACAAAGCAGAGTTCTTC
NM_152692.4         AGTCTTTGGGCTGCAGTAAAGGAGACTTGGACCAAACACTGTGACAAAGCAGAGTTCTTC
                    ************************************************************

HsCD00358302        AGTTCTGAAAATGTTAAAGTGTTTGAGTCAATTAATATGGACACAAATGACATGTGGTTA
NM_001011551.2      AGTTCTGAAAATGTTAAAGTGTTTGAGTCAATTAATATGGACACAAATGACATGTGGTTA
NM_152692.4         AGTTCTGAAAATGTTAAAGTGTTTGAGTCAATTAATATGGACACAAATGACATGTGGTTA
                    ************************************************************

HsCD00358302        ATGATGAGAAAAGCTTACAAATACGCCTTTGATAAGTATAGAGACCAATACAACTGGTTC
NM_001011551.2      ATGATGAGAAAAGCTTACAAATACGCCTTTGATAAGTATAGAGACCAATACAACTGGTTC
NM_152692.4         ATGATGAGAAAAGCTTACAAATACGCCTTTGATAAGTATAGAGACCAATACAACTGGTTC
                    ************************************************************

HsCD00358302        TTCCTTGCACGCCCCACTACGTTTGCTATCATTGAAAACCTAAAGTATTTTTTGTTAAAA
NM_001011551.2      TTCCTTGCACGCCCCACTACGTTTGCTATCATTGAAAACCTAAAGTATTTTTTGTTAAAA
NM_152692.4         TTCCTTGCACGCCCCACTACGTTTGCTATCATTGAAAACCTAAAGTATTTTTTGTTAAAA
                    ************************************************************

HsCD00358302        AAGGATCCATCACAGCCTTTCTATCTAGGCCACACTATAAAATCTGGAGACCTTGAATAT
NM_001011551.2      AAGGATCCATCACAGCCTTTCTATCTAGGCCACACTATAAAATCTGGAGACCTTGAATAT
NM_152692.4         AAGGATCCATCACAGCCTTTCTATCTAGGCCACACTATAAAATCTGGAGACCTTGAATAT
                    ************************************************************

HsCD00358302        GTGGGTATGGAAGGAGGAATTGTCTTAAGTGTAGAATCAATGAAAAGACTTAACAGCCTT
NM_001011551.2      GTGGGTATGGAAGGAGGAATTGTCTTAAGTGTAGAATCAATGAAAAGACTTAACAGCCTT
NM_152692.4         GTGGGTATGGAAGGAGGAATTGTCTTAAGTGTAGAATCAATGAAAAGACTTAACAGCCTT
                    ************************************************************

HsCD00358302        CTCAATATCCCAGAAAAGTGTCCTGAACAGGGAGGGATGATTTGGAAGATATCTGAAGAT
NM_001011551.2      CTCAATATCCCAGAAAAGTGTCCTGAACAGGGAGGGATGATTTGGAAGATATCTGAAGAT
NM_152692.4         CTCAATATCCCAGAAAAGTGTCCTGAACAGGGAGGGATGATTTGGAAGATATCTGAAGAT
                    ************************************************************

HsCD00358302        AAACAGCTAGCAGTTTGCCTGAAATATGCTGGAGTATTTGCAGAAAATGCAGAAGATGCT
NM_001011551.2      AAACAGCTAGCAGTTTGCCTGAAATATGCTGGAGTATTTGCAGAAAATGCAGAAGATGCT
NM_152692.4         AAACAGCTAGCAGTTTGCCTGAAATATGCTGGAGTATTTGCAGAAAATGCAGAAGATGCT
                    ************************************************************

HsCD00358302        GATGGAAAAGATGTATTTAATACCAAATCTGTTGGGCTTTCTATTAAAGAGGCAATGACT
NM_001011551.2      GATGGAAAAGATGTATTTAATACCAAATCTGTTGGGCTTTCTATTAAAGAGGCAATGACT
NM_152692.4         GATGGAAAAGATGTATTTAATACCAAATCTGTTGGGCTTTCTATTAAAGAGGCAATGACT
                    ************************************************************

HsCD00358302        TATCACCCCAACCAGGTAGTAGAAGGCTGTTGTTCAGATATGGCTGTTACTTTTAATGGA
NM_001011551.2      TATCACCCCAACCAGGTAGTAGAAGGCTGTTGTTCAGATATGGCTGTTACTTTTAATGGA
NM_152692.4         TATCACCCCAACCAGGTAGTAGAAGGCTGTTGTTCAGATATGGCTGTTACTTTTAATGGA
                    ************************************************************

HsCD00358302        CTGACTCCAAATCAGATGCATGTGATGATGTATGGGGTATACCGCCTTAGGGCATTTGGG
NM_001011551.2      CTGACTCCAAATCAGATGCATGTGATGATGTATGGGGTATACCGCCTTAGGGCATTTGGG
NM_152692.4         CTGACTCCAAATCAGATGCATGTGATGATGTATGGGGTATACCGCCTTAGGGCATTTGGG
                    ************************************************************

HsCD00358302        CATATTTTCAATGATGCATTGGTTTTCTTACCTCCAAATGGTTCTGACAATGACTAG
NM_001011551.2      CATATTTTCAATGATGCATTGGTTTTCTTACCTCCAAATGGTTCTGACAATGACTGA
NM_152692.4         CATATTTTCAATGATGCATTGGTTTTCTTACCTCCAAATGGTTCTGACAATGACTGA
                    *******************************************************..

Pathways

Reactome : Metabolism of proteins
Reactome : O-linked glycosylation
Reactome : O-linked glycosylation of mucins
Reactome : Post-translational protein modification

Protein

UniProt : Q96EU7
HPRD : 06462

Clone

Cloning Information : 560629

Organism


TAX_ID : 9606
Species Specific ID: 29071

Genome

Chromosome : X
Map Location : Xq24
Ensembl : ENSG00000171155

Reagents

Labome : C1GALT1C1-antibody