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ALG5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ALG5
Gene Name:  ALG5, dolichyl-phosphate beta-glucosyltransferase
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358304

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 891nts         Open reading frame : 1 to 891
Reference Sequence Alignment

ACAACTGCTACAAAAATGCCAGCACTCCATCGACATGAAGAAGAGAAATTCTTCTTAAAT
GCCAAAGGCCAGAAAGAAACTTTACCCAGCATATGGGACTCACCTACCAAACAACTTTCT
GTCGTTGTGCCTTCATACAATGAAGAAAAACGGTTGCCTGTGATGATGGATGAAGCTCTG
AGCTATCTAGAGAAGAGACAGAAACGAGATCCTGCGTTCACTTATGAAGTGATAGTAGTT
GATGATGGCAGTAAAGATCAGACCTCAAAGGTAGCTTTTAAATATTGCCAGAAATATGGA
AGTGACAAAGTACGTGTGATAACCCTGGTGAAGAATCGTGGAAAAGGTGGAGCGATTAGA
ATGGGTATATTCAGTTCTCGAGGAGAAAAGATCCTTATGGCAGATGCTGATGGAGCCACA
AAGTTTCCAGATGTTGAGAAATTAGAAAAGGGGCTAAATGATCTACAGCCTTGGCCTAAT
CAAATGGCTATAGCATGTGGATCTCGAGCTCATTTAGAAAAAGAATCAATTGCTCAGCGT
TCTTACTTCCGTACTCTTCTCATGTATGGGTTCCACTTTCTGGTGTGGTTCCTTTGTGTC
AAAGGAATCAGGGACACACAGTGTGGGTTCAAATTATTTACTCGAGAAGCAGCTTCACGG
ACGTTTTCATCTCTACACGTTGAACGATGGGCATTTGATGTAGAACTACTGTACATAGCA
CAGTTCTTTAAAATTCCAATAGCAGAAATTGCTGTCAACTGGACAGAAATTGAAGGTTCT
AAATTAGTTCCATTCTGGAGCTGGCTACAAATGGGTAAAGACCTACTTTTTATACGACTT
CGATATTTGACTGGTGCCTGGAGGCTTGAGCAAACTCGGAAAATGAATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,84

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ALG5
Symbol Nomenclature : ALG5
SYNONYM : RP11-421P11.2; bA421P11.2
Designation : Alg5, S. cerevisiae, homolog of|asparagine-linked glycosylation 5 homolog (S. cerevisiae, dolichyl-phosphate beta-glucosyltransferase)|asparagine-linked glycosylation 5 homolog (yeast, dolichyl-phosphate beta-glucosyltransferase)|asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog|asparagine-linked glycosylation protein 5 homolog|dolP-glucosyltransferase|dolichyl phosphate glucosyltransferase|dolichyl-phosphate beta-glucosyltransferase
Full Nomenclature : ALG5, dolichyl-phosphate beta-glucosyltransferase
GENEID : 29880
Locus Tag : HSPC149
GenBank Accession : BC012531
HGNC : 20266
MIM : 604565
Vega : OTTHUMG00000016741
Target GenBank: BC012531

 

Reference Sequence Alignment

  

HsCD00358304      ------------------------------------------------------------
NM_013338.4       ATGGCTCCGCTTCTGTTGCAGCTGGCGGTGCTCGGCGCGGCGCTGGCGGCCGCAGCCCTC
                                                                              

HsCD00358304      ------------------------ACAACTGCTACAAAAATGCCAGCACTCCATCGACAT
NM_013338.4       GTACTGATTTCCATCGTTGCATTTACAACTGCTACAAAAATGCCAGCACTCCATCGACAT
                                          ************************************

HsCD00358304      GAAGAAGAGAAATTCTTCTTAAATGCCAAAGGCCAGAAAGAAACTTTACCCAGCATATGG
NM_013338.4       GAAGAAGAGAAATTCTTCTTAAATGCCAAAGGCCAGAAAGAAACTTTACCCAGCATATGG
                  ************************************************************

HsCD00358304      GACTCACCTACCAAACAACTTTCTGTCGTTGTGCCTTCATACAATGAAGAAAAACGGTTG
NM_013338.4       GACTCACCTACCAAACAACTTTCTGTCGTTGTGCCTTCATACAATGAAGAAAAACGGTTG
                  ************************************************************

HsCD00358304      CCTGTGATGATGGATGAAGCTCTGAGCTATCTAGAGAAGAGACAGAAACGAGATCCTGCG
NM_013338.4       CCTGTGATGATGGATGAAGCTCTGAGCTATCTAGAGAAGAGACAGAAACGAGATCCTGCG
                  ************************************************************

HsCD00358304      TTCACTTATGAAGTGATAGTAGTTGATGATGGCAGTAAAGATCAGACCTCAAAGGTAGCT
NM_013338.4       TTCACTTATGAAGTGATAGTAGTTGATGATGGCAGTAAAGATCAGACCTCAAAGGTAGCT
                  ************************************************************

HsCD00358304      TTTAAATATTGCCAGAAATATGGAAGTGACAAAGTACGTGTGATAACCCTGGTGAAGAAT
NM_013338.4       TTTAAATATTGCCAGAAATATGGAAGTGACAAAGTACGTGTGATAACCCTGGTGAAGAAT
                  ************************************************************

HsCD00358304      CGTGGAAAAGGTGGAGCGATTAGAATGGGTATATTCAGTTCTCGAGGAGAAAAGATCCTT
NM_013338.4       CGTGGAAAAGGTGGAGCGATTAGAATGGGTATATTCAGTTCTCGAGGAGAAAAGATCCTT
                  ************************************************************

HsCD00358304      ATGGCAGATGCTGATGGAGCCACAAAGTTTCCAGATGTTGAGAAATTAGAAAAGGGGCTA
NM_013338.4       ATGGCAGATGCTGATGGAGCCACAAAGTTTCCAGATGTTGAGAAATTAGAAAAGGGGCTA
                  ************************************************************

HsCD00358304      AATGATCTACAGCCTTGGCCTAATCAAATGGCTATAGCATGTGGATCTCGAGCTCATTTA
NM_013338.4       AATGATCTACAGCCTTGGCCTAATCAAATGGCTATAGCATGTGGATCTCGAGCTCATTTA
                  ************************************************************

HsCD00358304      GAAAAAGAATCAATTGCTCAGCGTTCTTACTTCCGTACTCTTCTCATGTATGGGTTCCAC
NM_013338.4       GAAAAAGAATCAATTGCTCAGCGTTCTTACTTCCGTACTCTTCTCATGTATGGGTTCCAC
                  ************************************************************

HsCD00358304      TTTCTGGTGTGGTTCCTTTGTGTCAAAGGAATCAGGGACACACAGTGTGGGTTCAAATTA
NM_013338.4       TTTCTGGTGTGGTTCCTTTGTGTCAAAGGAATCAGGGACACACAGTGTGGGTTCAAATTA
                  ************************************************************

HsCD00358304      TTTACTCGAGAAGCAGCTTCACGGACGTTTTCATCTCTACACGTTGAACGATGGGCATTT
NM_013338.4       TTTACTCGAGAAGCAGCTTCACGGACGTTTTCATCTCTACACGTTGAACGATGGGCATTT
                  ************************************************************

HsCD00358304      GATGTAGAACTACTGTACATAGCACAGTTCTTTAAAATTCCAATAGCAGAAATTGCTGTC
NM_013338.4       GATGTAGAACTACTGTACATAGCACAGTTCTTTAAAATTCCAATAGCAGAAATTGCTGTC
                  ************************************************************

HsCD00358304      AACTGGACAGAAATTGAAGGTTCTAAATTAGTTCCATTCTGGAGCTGGCTACAAATGGGT
NM_013338.4       AACTGGACAGAAATTGAAGGTTCTAAATTAGTTCCATTCTGGAGCTGGCTACAAATGGGT
                  ************************************************************

HsCD00358304      AAAGACCTACTTTTTATACGACTTCGATATTTGACTGGTGCCTGGAGGCTTGAGCAAACT
NM_013338.4       AAAGACCTACTTTTTATACGACTTCGATATTTGACTGGTGCCTGGAGGCTTGAGCAAACT
                  ************************************************************

HsCD00358304      CGGAAAATGAATTAG
NM_013338.4       CGGAAAATGAATTAG
                  ***************

Pathways

Reactome : Asparagine N-linked glycosylation
Reactome : Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome : Metabolism of proteins
Reactome : Post-translational protein modification
Reactome : Synthesis of dolichyl-phosphate-glucose
Reactome : Synthesis of substrates in N-glycan biosythesis

Protein

UniProt : Q9Y673
HPRD : 05193

Clone

Cloning Information : 560643

Organism


TAX_ID : 9606
Species Specific ID: 29880

Genome

Chromosome : 13
Map Location : 13q13.3
Ensembl : ENSG00000120697

Reagents

Labome : ALG5-antibody