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GLT8D2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GLT8D2
Gene Name:  glycosyltransferase 8 domain containing 2
Sequence             
Original Clone ID: None
Keyword: partial cds (N-terminal deletion)
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: Glyco-enzyme clone collection
Description: All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments: for N-term fusion (no start codon)
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
University of Georgia
HsCD00358307

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 921nts         Open reading frame : 1 to 921
Reference Sequence Alignment

CCTGAAGAACTGGAAGAAGAGATTCCTGTGGTGATTTGTGCTGCAGCAGGGAGGATGGGT
GCCACTATGGCTGCCATCAATAGCATCTACAGCAACACTGACGCCAACATCTTGTTCTAT
GTAGTGGGACTCCGGAATACTCTGACTCGAATACGAAAATGGATTGAACATTCCAAACTG
AGAGAAATAAACTTTAAAATCGTGGAATTCAACCCGATGGTCCTCAAAGGGAAGATCAGA
CCAGACTCATCGAGGCCTGAATTGCTCCAGCCTCTGAACTTTGTTCGATTTTATCTCCCT
CTACTTATCCACCAACACGAGAAAGTCATCTATTTGGACGATGATGTAATTGTACAAGGT
GATATCCAAGAACTGTATGACACCACCTTGGCCCTGGGCCACGCGGCGGCTTTCTCAGAT
GACTGCGATTTGCCCTCTGCTCAGGACATAAACAGACTCGTGGGACTTCAGAACACATAT
ATGGGCTATCTGGACTACCGGAAGAAGGCCATCAAGGACCTTGGCATCAGCCCCAGCACC
TGCTCTTTCAATCCTGGTGTGATTGTTGCCAACATGACAGAATGGAAGCACCAGCGCATC
ACCAAGCAATTGGAGAAATGGATGCAAAAGAATGTGGAGGAAAACCTCTATAGCAGCTCC
CTGGGAGGAGGGGTGGCCACCTCCCCAATGCTGATTGTGTTTCATGGGAAATATTCCACA
ATTAACCCCCTGTGGCACATAAGGCACCTGGGCTGGAATCCAGATGCCAGATATTCGGAG
CATTTTCTGCAGGAAGCTAAATTACTCCACTGGAATGGAAGACATAAACCTTGGGACTTC
CCTAGTGTTCACAACGACTTATGGGAAAGCTGGTTTGTTCCTGACCCTGCAGGGATATTT
AAACTCAATCACCATAGCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
del@1,129

Discrepancy:
None
5' Linker Sequence:
AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATT
GATGAGCAATGCTTTTTTATAATGCCAATTTGTACAAAAAAGCAGGCTCT
GAAAACTTGTACTTTCAAGGC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTT
ATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTA
TT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GLT8D2
Symbol Nomenclature : GLT8D2
Designation : glycosyltransferase 8 domain-containing protein 2|gycosyltransferase
Full Nomenclature : glycosyltransferase 8 domain containing 2
GENEID : 83468
Locus Tag : UNQ1901/PRO4347
GenBank Accession : BC022343
HGNC : 24890
Vega : OTTHUMG00000170120
Target GenBank: BC022343

 

Reference Sequence Alignment

  

HsCD00358307        ------------------------------------------------------------
XM_005269171.1      ATGGCTCTGTTACGAAAAATTAATCAGGTGCTGCTGTTCCTTCTGATCGTGACCCTCTGT
NM_031302.3         ATGGCTCTGTTACGAAAAATTAATCAGGTGCTGCTGTTCCTTCTGATCGTGACCCTCTGT
                                                                                

HsCD00358307        ------------------------------------------------------------
XM_005269171.1      GTGATTCTGTATAAGAAAGTTCATAAGGGGACTGTGCCCAAGAATGACGCAGATGATGAA
NM_031302.3         GTGATTCTGTATAAGAAAGTTCATAAGGGGACTGTGCCCAAGAATGACGCAGATGATGAA
                                                                                

HsCD00358307        ---------CCTGAAGAACTGGAAGAAGAGATTCCTGTGGTGATTTGTGCTGCAGCAGGG
XM_005269171.1      TCCGAGACTCCTGAAGAACTGGAAGAAGAGATTCCTGTGGTGATTTGTGCTGCAGCAGGG
NM_031302.3         TCCGAGACTCCTGAAGAACTGGAAGAAGAGATTCCTGTGGTGATTTGTGCTGCAGCAGGG
                             ***************************************************

HsCD00358307        AGGATGGGTGCCACTATGGCTGCCATCAATAGCATCTACAGCAACACTGACGCCAACATC
XM_005269171.1      AGGATGGGTGCCACTATGGCTGCCATCAATAGCATCTACAGCAACACTGACGCCAACATC
NM_031302.3         AGGATGGGTGCCACTATGGCTGCCATCAATAGCATCTACAGCAACACTGACGCCAACATC
                    ************************************************************

HsCD00358307        TTGTTCTATGTAGTGGGACTCCGGAATACTCTGACTCGAATACGAAAATGGATTGAACAT
XM_005269171.1      TTGTTCTATGTAGTGGGACTCCGGAATACTCTGACTCGAATACGAAAATGGATTGAACAT
NM_031302.3         TTGTTCTATGTAGTGGGACTCCGGAATACTCTGACTCGAATACGAAAATGGATTGAACAT
                    ************************************************************

HsCD00358307        TCCAAACTGAGAGAAATAAACTTTAAAATCGTGGAATTCAACCCGATGGTCCTCAAAGGG
XM_005269171.1      TCCAAACTGAGAGAAATAAACTTTAAAATCGTGGAATTCAACCCGATGGTCCTCAAAGGG
NM_031302.3         TCCAAACTGAGAGAAATAAACTTTAAAATCGTGGAATTCAACCCGATGGTCCTCAAAGGG
                    ************************************************************

HsCD00358307        AAGATCAGACCAGACTCATCGAGGCCTGAATTGCTCCAGCCTCTGAACTTTGTTCGATTT
XM_005269171.1      AAGATCAGACCAGACTCATCGAGGCCTGAATTGCTCCAGCCTCTGAACTTTGTTCGATTT
NM_031302.3         AAGATCAGACCAGACTCATCGAGGCCTGAATTGCTCCAGCCTCTGAACTTTGTTCGATTT
                    ************************************************************

HsCD00358307        TATCTCCCTCTACTTATCCACCAACACGAGAAAGTCATCTATTTGGACGATGATGTAATT
XM_005269171.1      TATCTCCCTCTACTTATCCACCAACACGAGAAAGTCATCTATTTGGACGATGATGTAATT
NM_031302.3         TATCTCCCTCTACTTATCCACCAACACGAGAAAGTCATCTATTTGGACGATGATGTAATT
                    ************************************************************

HsCD00358307        GTACAAGGTGATATCCAAGAACTGTATGACACCACCTTGGCCCTGGGCCACGCGGCGGCT
XM_005269171.1      GTACAAGGTGATATCCAAGAACTGTATGACACCACCTTGGCCCTGGGCCACGCGGCGGCT
NM_031302.3         GTACAAGGTGATATCCAAGAACTGTATGACACCACCTTGGCCCTGGGCCACGCGGCGGCT
                    ************************************************************

HsCD00358307        TTCTCAGATGACTGCGATTTGCCCTCTGCTCAGGACATAAACAGACTCGTGGGACTTCAG
XM_005269171.1      TTCTCAGATGACTGCGATTTGCCCTCTGCTCAGGACATAAACAGACTCGTGGGACTTCAG
NM_031302.3         TTCTCAGATGACTGCGATTTGCCCTCTGCTCAGGACATAAACAGACTCGTGGGACTTCAG
                    ************************************************************

HsCD00358307        AACACATATATGGGCTATCTGGACTACCGGAAGAAGGCCATCAAGGACCTTGGCATCAGC
XM_005269171.1      AACACATATATGGGCTATCTGGACTACCGGAAGAAGGCCATCAAGGACCTTGGCATCAGC
NM_031302.3         AACACATATATGGGCTATCTGGACTACCGGAAGAAGGCCATCAAGGACCTTGGCATCAGC
                    ************************************************************

HsCD00358307        CCCAGCACCTGCTCTTTCAATCCTGGTGTGATTGTTGCCAACATGACAGAATGGAAGCAC
XM_005269171.1      CCCAGCACCTGCTCTTTCAATCCTGGTGTGATTGTTGCCAACATGACAGAATGGAAGCAC
NM_031302.3         CCCAGCACCTGCTCTTTCAATCCTGGTGTGATTGTTGCCAACATGACAGAATGGAAGCAC
                    ************************************************************

HsCD00358307        CAGCGCATCACCAAGCAATTGGAGAAATGGATGCAAAAGAATGTGGAGGAAAACCTCTAT
XM_005269171.1      CAGCGCATCACCAAGCAATTGGAGAAATGGATGCAAAAGAATGTGGAGGAAAACCTCTAT
NM_031302.3         CAGCGCATCACCAAGCAATTGGAGAAATGGATGCAAAAGAATGTGGAGGAAAACCTCTAT
                    ************************************************************

HsCD00358307        AGCAGCTCCCTGGGAGGAGGGGTGGCCACCTCCCCAATGCTGATTGTGTTTCATGGGAAA
XM_005269171.1      AGCAGCTCCCTGGGAGGAGGGGTGGCCACCTCCCCAATGCTGATTGTGTTTCATGGGAAA
NM_031302.3         AGCAGCTCCCTGGGAGGAGGGGTGGCCACCTCCCCAATGCTGATTGTGTTTCATGGGAAA
                    ************************************************************

HsCD00358307        TATTCCACAATTAACCCCCTGTGGCACATAAGGCACCTGGGCTGGAATCCAGATGCCAGA
XM_005269171.1      TATTCCACAATTAACCCCCTGTGGCACATAAGGCACCTGGGCTGGAATCCAGATGCCAGA
NM_031302.3         TATTCCACAATTAACCCCCTGTGGCACATAAGGCACCTGGGCTGGAATCCAGATGCCAGA
                    ************************************************************

HsCD00358307        TATTCGGAGCATTTTCTGCAGGAAGCTAAATTACTCCACTGGAATGGAAGACATAAACCT
XM_005269171.1      TATTCGGAGCATTTTCTGCAGGAAGCTAAATTACTCCACTGGAATGGAAGACATAAACCT
NM_031302.3         TATTCGGAGCATTTTCTGCAGGAAGCTAAATTACTCCACTGGAATGGAAGACATAAACCT
                    ************************************************************

HsCD00358307        TGGGACTTCCCTAGTGTTCACAACGACTTATGGGAAAGCTGGTTTGTTCCTGACCCTGCA
XM_005269171.1      TGGGACTTCCCTAGTGTTCACAACGACTTATGGGAAAGCTGGTTTGTTCCTGACCCTGCA
NM_031302.3         TGGGACTTCCCTAGTGTTCACAACGACTTATGGGAAAGCTGGTTTGTTCCTGACCCTGCA
                    ************************************************************

HsCD00358307        GGGATATTTAAACTCAATCACCATAGCTAG
XM_005269171.1      GGGATATTTAAACTCAATCACCATAGCTGA
NM_031302.3         GGGATATTTAAACTCAATCACCATAGCTGA
                    ****************************..

Protein

UniProt : Q9H1C3
HPRD : 14260

Clone

Cloning Information : 560669

Organism


TAX_ID : 9606
Species Specific ID: 83468

Genome

Chromosome : 12
Map Location : 12q
Ensembl : ENSG00000120820

Reagents

Labome : GLT8D2-antibody