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PPM1K (Homo sapiens) in pSGX3 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PPM1K
Gene Name:  protein phosphatase, Mg2+/Mn2+ dependent, 1K
Sequence             
Original Clone ID: 8700a2BCt11p1
Keyword: full-length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified. Actual 5' flanking sequence provided below. High number of silent and/or missense mutations in CDS. Reference sequence corrected.
Mutation/
Discrepancy :
No / No
Publications: PMID: 18058037
Title: Structural genomics of protein phosphatases
Authors: PSI
New York SGX Research Center for Structural Genomics
HsCD00370033

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 816nts         Open reading frame : 40 to 855
Reference Sequence Alignment

TTTTGTTTACTTTAAGAAGGAGATATACATATGTCCCTTAAAATCAGCTTGGAAAATGTG
GGGTGCGCCTCACAGATTGGCAAACGGAAAGAGAATGAAGATCGGTTTGACTTTGCTCAG
CTGACAGATGAGGTCCTGTACTTTGCAGTGTATGATGGACACGGTGGACCTGCAGCAGCT
GATTTCTGTCATACCCACATGGAGAAATGTATTATGGATTTGCTTCCTAAGGAGAAGAAC
TTGGAAACTCTGTTGACCTTGGCTTTTCTAGAAATAGATAAAGCCTTTTCGAGTCATGCC
CGCCTGTCTGCTGATGCAACTCTTCTGACCTCTGGGACTACTGCAACAGTAGCCCTATTG
CGAGATGGTATTGAACTGGTTGTAGCCAGTGTTGGGGACAGCCGGGCTATTTTGTGTAGA
AAAGGAAAACCCATGAAGCTGACCATTGACCATACTCCAGAAAGAAAAGATGAAAAAGAA
AGGATCAAGAAATGTGGTGGTTTTGTAGCTTGGAATAGTTTGGGGCAGCCTCACGTAAAT
GGCAGGCTTGCAATGACAAGAAGTATTGGAGATTTGGACCTTAAGACCAGTGGTGTCATA
GCAGAACCTGAAACTAAGAGGATTAAGTTACATCATGCTGATGACAGCTTCCTGGTCCTC
ACCACAGATGGAATTAACTTCATGGTGAATAGTCAAGAGATTTGTGACTTTGTCAATCAG
TGCCATGATCCCAACGAAGCAGCCCATGCGGTGACTGAACAGGCAATACAGTACGGTACT
GAGGATAACAGTACTGCAGTAGTAGTGCCTTTTGGTGCCTGGGGAAAAGAAGGGCATCAT
CACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCA
CCACCA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-8700a
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPM1K
Symbol Nomenclature : PPM1K
SYNONYM : BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP; UG0882E07
Designation : PP2C domain-containing protein phosphatase 1K|PP2C-kappa|PP2C-type mitochondrial phosphoprotein phosphatase|branched-chain alpha-ketoacid dehydrogenase phosphatase|protein phosphatase 1K, mitochondrial|protein phosphatase 2C kappa
Full Nomenclature : protein phosphatase, Mg2+/Mn2+ dependent, 1K
GENEID : 152926
GI : 167466276
GenBank Accession : NM_152542
HGNC : 25415
MIM : 611065
Vega : OTTHUMG00000130952
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00370033      ------------------------------------------------------------
NM_152542.4       ATGTCAACAGCTGCCTTAATTACTTTGGTCAGAAGTGGTGGGAACCAGGTGAGAAGGAGA
                                                                              

HsCD00370033      ------------------------------------------------------------
NM_152542.4       GTGCTGCTAAGCTCCCGCCTGCTGCAGGACGACAGGCGGGTGACACCCACGTGCCACAGC
                                                                              

HsCD00370033      ------------------------------------------------------------
NM_152542.4       TCCACTTCAGAGCCTAGGTGTTCTCGGTTTGACCCAGATGGTAGTGGGAGTCCAGCTACC
                                                                              

HsCD00370033      ---------------------------------------------TTTTGTTTACTTTAA
NM_152542.4       TGGGACAATTTTGGGATCTGGGATAACCGCATTGATGAGCCAATTCTGCTGCCACCCAGC
                                                                *      **  :..

HsCD00370033      GAAGGAGATATACATATGTCCCTTAAAATCAGCTTGGAAAATGTGGGGTGCGCCTCACAG
NM_152542.4       ATTAAGTATGGCAAGCCAATTCCCAAAATCAGCTTGGAAAATGTGGGGTGCGCCTCACAG
                  .::... **. ..* . .:  *  ************************************

HsCD00370033      ATTGGCAAACGGAAAGAGAATGAAGATCGGTTTGACTTTGCTCAGCTGACAGATGAGGTC
NM_152542.4       ATTGGCAAACGGAAAGAGAATGAAGATCGGTTTGACTTCGCTCAGCTGACAGATGAGGTC
                  ************************************** *********************

HsCD00370033      CTGTACTTTGCAGTGTATGATGGACACGGTGGACCTGCAGCAGCTGATTTCTGTCATACC
NM_152542.4       CTGTACTTTGCAGTGTATGATGGACACGGTGGACCTGCAGCAGCTGATTTCTGTCATACC
                  ************************************************************

HsCD00370033      CACATGGAGAAATGTATTATGGATTTGCTTCCTAAGGAGAAGAACTTGGAAACTCTGTTG
NM_152542.4       CACATGGAGAAATGTATTATGGATTTGCTTCCTAAGGAGAAGAACTTGGAAACTCTGTTG
                  ************************************************************

HsCD00370033      ACCTTGGCTTTTCTAGAAATAGATAAAGCCTTTTCGAGTCATGCCCGCCTGTCTGCTGAT
NM_152542.4       ACCTTGGCTTTTCTAGAAATAGATAAAGCCTTTTCGAGTCATGCCCGCCTGTCTGCTGAT
                  ************************************************************

HsCD00370033      GCAACTCTTCTGACCTCTGGGACTACTGCAACAGTAGCCCTATTGCGAGATGGTATTGAA
NM_152542.4       GCAACTCTTCTGACCTCTGGGACTACTGCAACAGTAGCCCTATTGCGAGATGGTATTGAA
                  ************************************************************

HsCD00370033      CTGGTTGTAGCCAGTGTTGGGGACAGCCGGGCTATTTTGTGTAGAAAAGGAAAACCCATG
NM_152542.4       CTGGTTGTAGCCAGTGTTGGGGACAGCCGGGCTATTTTGTGTAGAAAAGGAAAACCCATG
                  ************************************************************

HsCD00370033      AAGCTGACCATTGACCATACTCCAGAAAGAAAAGATGAAAAAGAAAGGATCAAGAAATGT
NM_152542.4       AAGCTGACCATTGACCATACTCCAGAAAGAAAAGATGAAAAAGAAAGGATCAAGAAATGT
                  ************************************************************

HsCD00370033      GGTGGTTTTGTAGCTTGGAATAGTTTGGGGCAGCCTCACGTAAATGGCAGGCTTGCAATG
NM_152542.4       GGTGGTTTTGTAGCTTGGAATAGTTTGGGGCAGCCTCACGTAAATGGCAGGCTTGCAATG
                  ************************************************************

HsCD00370033      ACAAGAAGTATTGGAGATTTGGACCTTAAGACCAGTGGTGTCATAGCAGAACCTGAAACT
NM_152542.4       ACAAGAAGTATTGGAGATTTGGACCTTAAGACCAGTGGTGTCATAGCAGAACCTGAAACT
                  ************************************************************

HsCD00370033      AAGAGGATTAAGTTACATCATGCTGATGACAGCTTCCTGGTCCTCACCACAGATGGAATT
NM_152542.4       AAGAGGATTAAGTTACATCATGCTGATGACAGCTTCCTGGTCCTCACCACAGATGGAATT
                  ************************************************************

HsCD00370033      AACTTCATGGTGAATAGTCAAGAGATTTGTGACTTTGTCAATCAGTGCCATGATCCCAAC
NM_152542.4       AACTTCATGGTGAATAGTCAAGAGATTTGTGACTTTGTCAATCAGTGCCATGATCCCAAC
                  ************************************************************

HsCD00370033      GAAGCAGCCCATGCGGTGACTGAACAGGCAATACAGTACGGTACTGAGGATAACAGTACT
NM_152542.4       GAAGCAGCCCATGCGGTGACTGAACAGGCAATACAGTACGGTACTGAGGATAACAGTACT
                  ************************************************************

HsCD00370033      GCAGTAGTAGTGCCTTTTGGTGCCTGGGGAAAAGAAGGGCATCATCACCATCACCACTGA
NM_152542.4       GCAGTAGTAGTGCCTTTTGGTGCCTGGGGAAAATATAAGAACTCTGAAATCAACTTCTCA
                  ********************************* *:..*.*  .* *..: .** :** *

HsCD00370033      TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCA
NM_152542.4       TTCAGCAGAAGCTTTGCCTCCAGTGGACGATGGGCCTGA------------
                  * *...: ...  *      ...:* : *. *   *: :            

Protein

PDB - Structural Annotation : 2IQ1
SMART domain : PP2C_SIG : Sigma factor PP2C-like phosphatases
SMART domain : PP2Cc : Serine/threonine phosphatases, family 2C, catalytic domain
Structural Annotation Wiki : TOPSAN
UniProt : Q8N3J5
HPRD : 15167
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 494216
HIP Master Clone ID : 407896
Original Clone ID : 8700a2BCt11p1

Experimental

Structural Biology Knowledgebase : SBKB (2IQ1)
TargetTrack - Experimental data : NYSGXRC-8700a

Organism


TAX_ID : 9606
Species Specific ID: 152926

Genome

Chromosome : 4
Map Location : 4q22.1
Ensembl : ENSG00000163644

Reagents

Labome : PPM1K-antibody