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CTDSP2 (Homo sapiens) in pSGX4 (His and Smt3-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CTDSP2
Gene Name:  CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2
Sequence             
Original Clone ID: 8717a3BSt5p1
Keyword: partial length cds
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX4               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified.
Mutation/
Discrepancy :
No / No
Publications: PMID: 18058037
Title: Structural genomics of protein phosphatases
Authors: PSI
New York SGX Research Center for Structural Genomics
HsCD00370061

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 558nts         Open reading frame : 51 to 608
Reference Sequence Alignment

AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTGGGACCTGCC
TGCTTCCAGAGGTGACAGAGGAAGATCAAGGAAGGATCTGTGTGGTCATTGACCTCGATG
AAACCCTTGTGCATAGCTCCTTTAAGCCAATCAACAATGCTGACTTCATAGTGCCTATAG
AGATTGAGGGGACCACTCACCAGGTGTATGTGCTCAAGAGGCCTTATGTGGATGAGTTCC
TGAGACGCATGGGGGAACTCTTTGAATGTGTTCTCTTCACTGCCAGCCTGGCCAAGTATG
CCGACCCTGTGACAGACCTGCTGGACCGGTGTGGGGTGTTCCGGGCCCGCCTATTCCGTG
AGTCTTGCGTGTTCCACCAGGGCTGCTACGTCAAGGACCTCAGCCGCCTGGGGAGGGACC
TGAGAAAGACCCTCATCCTGGACAACTCGCCTGCTTCTTACATATTCCACCCCGAGAATG
CAGTGCCTGTGCAGTCCTGGTTTGATGACATGGCAGACACTGAGTTGCTGAACCTGATCC
CAATCTTTGAGGAGCTGAGCGGAGCAGAGGACGTCTACACCAGCCTTGGGCAGCTGCGGG
CCCCTTAGCCTGCCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAGAGAACAGATTGGTGGATCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-8717a
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX4

pSGX4 in advanced viewed

Synonyms: pSGX-4, pSGX4_BS
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.
Size (bp): 5611
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
TOPO recognition site TOPO TOPO cloning site 5435 5444
bacterial origin ColE ColE1 bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4984 5003
protease cleavage site ULP site ULP cleavage site (recognizes the tertiary structure of SUMO) 5140 5140
repressor protein gene LacI lacI cds 3515 4351
ribosome binding site RBS ribosome binding site (RBS) 5062 5068
selectable marker KanR kanamycin resistance gene 563 1375
tag Smt3 Smt3 tag 5140 5424
tag His 6xHis tag 5083 5100
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 termination sequence 5539 5585

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CTDSP2
Symbol Nomenclature : CTDSP2
SYNONYM : OS4; PSR2; SCP2
Designation : NLI-interacting factor 2|carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2|conserved gene amplified in osteosarcoma|nuclear LIM interactor-interacting factor 2|protein OS-4|small C-terminal domain phosphatase 2|small CTD phosphatase 2
Full Nomenclature : CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2
GENEID : 10106
GI : 93004102
GenBank Accession : NM_005730
HGNC : 17077
MIM : 608711
Vega : OTTHUMG00000170483
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00370061        ------------------------------------------------------------
NM_005730.3         ATGGAACACGGCTCCATCATCACCCAGGCGCGGAGGGAAGACGCCCTGGTGCTCACCAAG
XM_005268556.1      ATGGAACACGGCTCCATCATCACCCAGGCGCGGAGGGAAGACGCCCTGGTGCTCACCAAG
                                                                                

HsCD00370061        ------------------------------------------------------------
NM_005730.3         CAAGGCCTGGTCTCCAAGTCCTCTCCTAAGAAGCCTCGTGGACGTAACATCTTCAAGGCC
XM_005268556.1      CAAGGCCTGGTCTCCAAGTCCTCTCCTAAGAAGCCTCGTGGACGTAACATCTTCAAGGCC
                                                                                

HsCD00370061        ------------------------------------------------------------
NM_005730.3         CTTTTCTGCTGTTTTCGCGCCCAGCATGTTGGCCAGTCAAGTTCCTCCACTGAGCTCGCT
XM_005268556.1      CTTTTCTGCTGTTTTCGCGCCCAGCATGTTGGCCAGTCAAGTTCCTCCACTGAGCTCGCT
                                                                                

HsCD00370061        -------AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTG-----------
NM_005730.3         GCGTATAAGGAGGAAGCAAACACCATTGCTAAGTCGGATCTGCTCCAGTGTCTCCAGTAC
XM_005268556.1      GCGTATAAGGAGGAAGCAAACACCATTGCTAAGTCGGATCTGCTCCAGTGTCTCCAGTAC
                           **** .*.*.:*: *  .: ***.* : .**:*:*.*  .             

HsCD00370061        ----------------------------GATCCCTTGGGACCTGCCTGCTTCCAGAGGTG
NM_005730.3         CAGTTCTACCAG------------------ATCCCAGGGACCTGCCTGCTCCCAGAGGTG
XM_005268556.1      CAGTTCTACCAGGTACGTGACTCTGGCCTGATCCCAGGGACCTGCCTGCTCCCAGAGGTG
                                                  : ** :************** *********

HsCD00370061        ACAGAGGAAGATCAAGGAAGGATCTGTGTGGTCATTGACCTCGATGAAACCCTTGTGCAT
NM_005730.3         ACAGAGGAAGATCAAGGAAGGATCTGTGTGGTCATTGACCTCGATGAAACCCTTGTGCAT
XM_005268556.1      ACAGAGGAAGATCAAGGAAGGATCTGTGTGGTCATTGACCTCGATGAAACCCTTGTGCAT
                    ************************************************************

HsCD00370061        AGCTCCTTTAAGCCAATCAACAATGCTGACTTCATAGTGCCTATAGAGATTGAGGGGACC
NM_005730.3         AGCTCCTTTAAGCCAATCAACAATGCTGACTTCATAGTGCCTATAGAGATTGAGGGGACC
XM_005268556.1      AGCTCCTTTAAGCCAATCAACAATGCTGACTTCATAGTGCCTATAGAGATTGAGGGGACC
                    ************************************************************

HsCD00370061        ACTCACCAGGTGTATGTGCTCAAGAGGCCTTATGTGGATGAGTTCCTGAGACGCATGGGG
NM_005730.3         ACTCACCAGGTGTATGTGCTCAAGAGGCCTTATGTGGATGAGTTCCTGAGACGCATGGGG
XM_005268556.1      ACTCACCAGGTGTATGTGCTCAAGAGGCCTTATGTGGATGAGTTCCTGAGACGCATGGGG
                    ************************************************************

HsCD00370061        GAACTCTTTGAATGTGTTCTCTTCACTGCCAGCCTGGCCAAGTATGCCGACCCTGTGACA
NM_005730.3         GAACTCTTTGAATGTGTTCTCTTCACTGCCAGCCTGGCCAAGTATGCCGACCCTGTGACA
XM_005268556.1      GAACTCTTTGAATGTGTTCTCTTCACTGCCAGCCTGGCCAAGTATGCCGACCCTGTGACA
                    ************************************************************

HsCD00370061        GACCTGCTGGACCGGTGTGGGGTGTTCCGGGCCCGCCTATTCCGTGAGTCTTGCGTGTTC
NM_005730.3         GACCTGCTGGACCGGTGTGGGGTGTTCCGGGCCCGCCTATTCCGTGAGTCTTGCGTGTTC
XM_005268556.1      GACCTGCTGGACCGGTGTGGGGTGTTCCGGGCCCGCCTATTCCGTGAGTCTTGCGTGTTC
                    ************************************************************

HsCD00370061        CACCAGGGCTGCTACGTCAAGGACCTCAGCCGCCTGGGGAGGGACCTGAGAAAGACCCTC
NM_005730.3         CACCAGGGCTGCTACGTCAAGGACCTCAGCCGCCTGGGGAGGGACCTGAGAAAGACCCTC
XM_005268556.1      CACCAGGGCTGCTACGTCAAGGACCTCAGCCGCCTGGGGAGGGACCTGAGAAAGACCCTC
                    ************************************************************

HsCD00370061        ATCCTGGACAACTCGCCTGCTTCTTACATATTCCACCCCGAGAATGCAGTGCCTGTGCAG
NM_005730.3         ATCCTGGACAACTCGCCTGCTTCTTACATATTCCACCCCGAGAATGCAGTGCCTGTGCAG
XM_005268556.1      ATCCTGGACAACTCGCCTGCTTCTTACATATTCCACCCCGAGAATGCAGTGCCTGTGCAG
                    ************************************************************

HsCD00370061        TCCTGGTTTGATGACATGGCAGACACTGAGTTGCTGAACCTGATCCCAATCTTTGAGGAG
NM_005730.3         TCCTGGTTTGATGACATGGCAGACACTGAGTTGCTGAACCTGATCCCAATCTTTGAGGAG
XM_005268556.1      TCCTGGTTTGATGACATGGCAGACACTGAGTTGCTGAACCTGATCCCAATCTTTGAGGAG
                    ************************************************************

HsCD00370061        CTGAGCGGAGCAGAGGACGTCTACACCAGCCTTGGGCAGCTGCGGGCCCCTTAGCCTGCC
NM_005730.3         CTGAGCGGAGCAGAGGACGTCTACACCAGCCTTGGGCAGCTGCGGGCCCCTTAG------
XM_005268556.1      CTGAGCGGAGCAGAGGACGTCTACACCAGCCTTGGGCAGCTGCGGGCCCCTTAG------
                    ******************************************************      

HsCD00370061        C
NM_005730.3         -
XM_005268556.1      -
                     

Pathways

NCI : BMP receptor signaling
NCI : Coregulation of Androgen receptor activity
NCI : Regulation of cytoplasmic and nuclear SMAD2/3 signaling
Reactome : IRE1alpha activates chaperones
Reactome : Metabolism of proteins
Reactome : Unfolded Protein Response (UPR)
Reactome : XBP1(S) activates chaperone genes

Protein

PDB - Structural Annotation : 2Q5E
SMART domain : CPDc : catalytic domain of ctd-like phosphatases
Structural Annotation Wiki : TOPSAN
UniProt : O14595
HPRD : 12281
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 494324
HIP Master Clone ID : 408004
Original Clone ID : 8717a3BSt5p1

Experimental

Structural Biology Knowledgebase : SBKB (2Q5E)
TargetTrack - Experimental data : NYSGXRC-8717a

Organism


TAX_ID : 9606
Species Specific ID: 10106

Genome

Chromosome : 12
Map Location : 12q14.1
Ensembl : ENSG00000175215

Reagents

Labome : CTDSP2-antibody