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gmuG (B. subtilis subsp. subtilis str. 168 ) in pSGX3

TOP

Explanation of Terms


Gene: Gene Symbol:  gmuG
Gene Name:  mannan endo-1,4-beta-mannosidase
Sequence             
Original Clone ID: 11098a1BCt5p1
Keyword: full-length cds
Species: Bacillus subtilis subsp. subtilis str. 168
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified. Actual 5' flanking sequence provided below.
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: PSI
New York SGX Research Center for Structural Genomics
BsCD00370437

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1050nts        
Open reading frame : 9 to 1058

TCCCTTGCAAACCAATTGAAGCGCATACTGTGTCGCCTGTGAATCCTAATGCCCAGCAGA
CAACAAAAACAGTGATGAACTGGCTTGCGCACCTGCCGAACCGAACGGAAAACAGAGTCC
TTTCCGGAGCGTTCGGAGGTTACAGCCATGACACATTTTCTATGGCTGAGGCTGATAGAA
TCCGAAGCGCCACCGGGCAATCGCCTGCTATTTATGGCTGCGATTATGCCAGAGGATGGC
TTGAAACAGCAAATATTGAAGATTCAATAGATGTAAGCTGCAACGGCGATTTAATGTCGT
ATTGGAAAAATGGCGGAATTCCGCAAATCAGTTTGCACCTGGCGAACCCTGCTTTTCAGT
CAGGGCATTTTAAAACACCGATTACAAATGATCAGTATAAAAAAATACTAGATTCTTCAA
CAGTAGAAGGAAAGCGGCTAAATGCCATGCTCAGCAAAATTGCTGACGGACTTCAAGAGT
TGGAGAACCAAGGTGTGCCTGTTCTGTTCAGGCCGCTGCATGAAATGAACGGCGAATGGT
TTTGGTGGGGACTCACATCATATAACCAAAAGGATAATGAAAGAATCTCTCTATATAAAC
AGCTCTACAAGAAAATCTATCATTATATGACCGACACAAGAGGACTTGATCATTTGATTT
GGGTTTACTCTCCCGACGCCAACCGAGATTTTAAAACTGATTTTTACCCGGGCGCGTCTT
ACGTGGATATTGTCGGATTAGATGCGTATTTTCAAGATGCCTACTCGATCAATGGATACG
ATCAGCTAACAGCGCTTAATAAACCATTTGCTTTTACAGAAGTCGGCCCGCAAACAGCAA
ACGGCAGCTTCGATTACAGCCTGTTCATCAATGCAATAAAACAAAAATATCCTAAAACCA
TTTACTTTCTGGCATGGAATGATGAATGGAGCGCAGCAGTAAACAAGGGTGCTTCAGCTT
TATATCATGACAGCTGGACACTCAACAAGGGAGAAATATGGAATGGTGATTCTTTAACGC
CAATCGTTGAGGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGAC
AAGCTTGCGGCCGCACTCGAGCACCACCA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
N153K; A158S; A160V

Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11098a
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : gmuG
GENEID : 938034
Locus Tag : BSU05880
GI : 225184779
GenBank Accession : CAB12407.1
Target GenBank: None

 

Protein

PDB - Structural Annotation : 3CBW
Structural Annotation Wiki : TOPSAN
UniProt : O05512
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 495447
HIP Master Clone ID : 409127
Original Clone ID : 11098a1BCt5p1

Experimental

Structural Biology Knowledgebase : SBKB (3CBW)
TargetTrack - Experimental data : NYSGXRC-11098a

Organism


TAX_ID : 224308
Species Specific ID: 938034

Reagents

Labome : gmuG-cDNA