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KPNA1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KPNA1
Gene Name:  karyopherin alpha 1 (importin alpha 5)
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH025442.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003759

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1617nts         Open reading frame : 1 to 1617
Reference Sequence Alignment

ATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAAT
CCCGATGAGATGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGA
GAAGAGCAGTTATTCAAGCGGAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAA
GTTATGTCAGATGGAGGCTTTCATGAGGCTCAGATTAATAACATGGAGATGGCACCAGGT
GGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCAAAAGCCCAGAGCAACAGCTT
TCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCCTATTGATGAA
GTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAAT
TGTACACTGCAGTTTGAATCAGCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTT
CAGACCCGAATTGTGATTCAGGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCA
GAGTTTGAAGATGTCCAGGAACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGT
ACCATGTGCAGGGACTATGTCTTAGACTGCAATATCCTTCCCCCTCTTTTGCAGTTATTT
TCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTATGGGCTTTGTCTAATCTCTGT
AGAGGGAAAAGTCCACCTCCAGAATTTGCAAAGGTTTCTCCATGTCTGAATGTGCTTTCC
TGGTTGCTGTTTGTCAGTGACACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATAT
CTATCAGATGGACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGA
CTTGTGGAACTGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTG
GGAAACATTGTCACAGGGGATGATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTG
CAGAGTTTATTGCATTTGCTGAGTAGCCCAAAGGAATCTATCAAAAAGGAAGCATGTTGG
ACGATATCTAATATTACAGCTGGAAATAGGGCACAGATCCAGACTGTGATAGATGCCAAC
ATTTTCCCAGCCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAGAAAAGAAGCA
GCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTA
GAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTA
CAGGTTGCCCTAAATGGCTTGGAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGG
AATGGCACTGGCATTAACCCTTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAA
ATTGAGTTCTTACAGAGTCATGAAAACCAGGAGATCTACCAAAAGGCCTTTGATCTTATT
GAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTGCACCCCAGGTTGACCTTAAC
CAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGGAAGGTTTCCAGCTTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1794
Start on reference sequence 178
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KPNA1
Symbol Nomenclature : KPNA1
SYNONYM : IPOA5; NPI-1; RCH2; SRP1
Designation : RAG cohort protein 2|SRP1-beta|importin alpha 5|importin subunit alpha-1|importin subunit alpha-5|importin-alpha-S1|karyopherin subunit alpha-1|nucleoprotein interactor 1|recombination activating gene cohort 2
Full Nomenclature : karyopherin alpha 1 (importin alpha 5)
GENEID : 3836
GI : 60656076
GenBank Accession : AY889811
HGNC : 6394
MIM : 600686
Vega : OTTHUMG00000159487
Target GenBank: BC003009

 

Reference Sequence Alignment

  

HsCD00003759      ATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAAT
NM_002264.3       ATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAAT
                  ************************************************************

HsCD00003759      CCCGATGAGATGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGA
NM_002264.3       CCCGATGAGATGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGA
                  ************************************************************

HsCD00003759      GAAGAGCAGTTATTCAAGCGGAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAA
NM_002264.3       GAAGAGCAGTTATTCAAGCGGAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAA
                  ************************************************************

HsCD00003759      GTTATGTCAGATGGAGGCTTTCATGAGGCTCAGATTAATAACATGGAGATGGCACCAGGT
NM_002264.3       GTTATGTCAGATGGAGGCTTTCATGAGGCTCAGATTAGTAACATGGAGATGGCACCAGGT
                  *************************************.**********************

HsCD00003759      GGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCAAAAGCCCAGAGCAACAGCTT
NM_002264.3       GGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCAAAAGCCCAGAGCAACAGCTT
                  ************************************************************

HsCD00003759      TCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCCTATTGATGAA
NM_002264.3       TCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCCTATTGATGAA
                  ************************************************************

HsCD00003759      GTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAAT
NM_002264.3       GTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAAT
                  ************************************************************

HsCD00003759      TGTACACTGCAGTTTGAATCAGCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTT
NM_002264.3       TGTACACTGCAGTTTGAATCAGCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTT
                  ************************************************************

HsCD00003759      CAGACCCGAATTGTGATTCAGGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCA
NM_002264.3       CAGACCCGAATTGTGATTCAGGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCA
                  ************************************************************

HsCD00003759      GAGTTTGAAGATGTCCAGGAACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGT
NM_002264.3       GAGTTTGAAGATGTCCAGGAACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGT
                  ************************************************************

HsCD00003759      ACCATGTGCAGGGACTATGTCTTAGACTGCAATATCCTTCCCCCTCTTTTGCAGTTATTT
NM_002264.3       ACCATGTGCAGGGACTATGTCTTAGACTGCAATATCCTTCCCCCTCTTTTGCAGTTATTT
                  ************************************************************

HsCD00003759      TCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTATGGGCTTTGTCTAATCTCTGT
NM_002264.3       TCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTATGGGCTTTGTCTAATCTCTGT
                  ************************************************************

HsCD00003759      AGAGGGAAAAGTCCACCTCCAGAATTTGCAAAGGTTTCTCCATGTCTGAATGTGCTTTCC
NM_002264.3       AGAGGGAAAAGTCCACCTCCAGAATTTGCAAAGGTTTCTCCATGTCTGAATGTGCTTTCC
                  ************************************************************

HsCD00003759      TGGTTGCTGTTTGTCAGTGACACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATAT
NM_002264.3       TGGTTGCTGTTTGTCAGTGACACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATAT
                  ************************************************************

HsCD00003759      CTATCAGATGGACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGA
NM_002264.3       CTATCAGATGGACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGA
                  ************************************************************

HsCD00003759      CTTGTGGAACTGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTG
NM_002264.3       CTTGTGGAACTGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTG
                  ************************************************************

HsCD00003759      GGAAACATTGTCACAGGGGATGATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTG
NM_002264.3       GGAAACATTGTCACAGGGGATGATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTG
                  ************************************************************

HsCD00003759      CAGAGTTTATTGCATTTGCTGAGTAGCCCAAAGGAATCTATCAAAAAGGAAGCATGTTGG
NM_002264.3       CAGAGTTTATTGCATTTGCTGAGTAGCCCAAAGGAATCTATCAAAAAGGAAGCATGTTGG
                  ************************************************************

HsCD00003759      ACGATATCTAATATTACAGCTGGAAATAGGGCACAGATCCAGACTGTGATAGATGCCAAC
NM_002264.3       ACGATATCTAATATTACAGCTGGAAATAGGGCACAGATCCAGACTGTGATAGATGCCAAC
                  ************************************************************

HsCD00003759      ATTTTCCCAGCCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAGAAAAGAAGCA
NM_002264.3       ATTTTCCCAGCCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAGAAAAGAAGCA
                  ************************************************************

HsCD00003759      GCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTA
NM_002264.3       GCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTA
                  ************************************************************

HsCD00003759      GAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTA
NM_002264.3       GAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTA
                  ************************************************************

HsCD00003759      CAGGTTGCCCTAAATGGCTTGGAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGG
NM_002264.3       CAGGTTGCCCTAAATGGCTTGGAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGG
                  ************************************************************

HsCD00003759      AATGGCACTGGCATTAACCCTTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAA
NM_002264.3       AATGGCACTGGCATTAACCCTTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAA
                  ************************************************************

HsCD00003759      ATTGAGTTCTTACAGAGTCATGAAAACCAGGAGATCTACCAAAAGGCCTTTGATCTTATT
NM_002264.3       ATTGAGTTCTTACAGAGTCATGAAAACCAGGAGATCTACCAAAAGGCCTTTGATCTTATT
                  ************************************************************

HsCD00003759      GAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTGCACCCCAGGTTGACCTTAAC
NM_002264.3       GAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTGCACCCCAGGTTGACCTTAAC
                  ************************************************************

HsCD00003759      CAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGGAAGGTTTCCAGCTTTAG
NM_002264.3       CAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGGAAGGTTTCCAGCTTTGA
                  *******************************************************..

Pathways

NCI : Class I PI3K signaling events mediated by Akt
Reactome : Activation of DNA fragmentation factor
Reactome : Antiviral mechanism by IFN-stimulated genes
Reactome : Apoptosis
Reactome : Apoptosis induced DNA fragmentation
Reactome : Apoptotic execution phase
Reactome : Cytokine Signaling in Immune system
Reactome : Disease
Reactome : HIV Infection
Reactome : Host Interactions of HIV factors
Reactome : ISG15 antiviral mechanism
Reactome : Immune System
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Interactions of Vpr with host cellular proteins
Reactome : Interferon Signaling
Reactome : Transport of Ribonucleoproteins into the Host Nucleus
Reactome : Vpr-mediated nuclear import of PICs

Protein

SMART domain : ARM : Armadillo/beta-catenin-like repeats
UniProt : P52294
HPRD : 02819

Clone

Cloning Information : 25442
HIP Master Clone ID : 55818
Original Clone ID : FLH025442.01X

Organism


TAX_ID : 9606
Species Specific ID: 3836

Genome

Chromosome : 3
Map Location : 3q21
Ensembl : ENSG00000114030

Reagents

Labome : KPNA1-antibody