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CSTF1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CSTF1
Gene Name:  cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH015272.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003781

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1296nts         Open reading frame : 1 to 1296
Reference Sequence Alignment

ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
GCGCGGTTTTGGTACCGGAGATCGACCACTGACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1394
Start on reference sequence 99
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSTF1
Symbol Nomenclature : CSTF1
SYNONYM : CstF-50; CstFp50
Designation : CF-1 50 kDa subunit|CSTF 50 kDa subunit|cleavage stimulation factor 50 kDa subunit|cleavage stimulation factor subunit 1
Full Nomenclature : cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
GENEID : 1477
Locus Tag : RP5-1167H4.1
GI : 60653022
GenBank Accession : AY892289
HGNC : 2483
MIM : 600369
Vega : OTTHUMG00000032791
Target GenBank: BC007425

 

Reference Sequence Alignment

  

HsCD00003781        ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
NM_001033522.1      ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
NM_001324.2         ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
NM_001033521.1      ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC
                    ************************************************************

HsCD00003781        CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
NM_001033522.1      CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
NM_001324.2         CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
NM_001033521.1      CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT
                    ************************************************************

HsCD00003781        CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
NM_001033522.1      CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
NM_001324.2         CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
NM_001033521.1      CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC
                    ************************************************************

HsCD00003781        GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
NM_001033522.1      GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
NM_001324.2         GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
NM_001033521.1      GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG
                    ************************************************************

HsCD00003781        ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
NM_001033522.1      ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
NM_001324.2         ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
NM_001033521.1      ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA
                    ************************************************************

HsCD00003781        ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
NM_001033522.1      ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
NM_001324.2         ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
NM_001033521.1      ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA
                    ************************************************************

HsCD00003781        CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
NM_001033522.1      CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
NM_001324.2         CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
NM_001033521.1      CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG
                    ************************************************************

HsCD00003781        TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
NM_001033522.1      TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
NM_001324.2         TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
NM_001033521.1      TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG
                    ************************************************************

HsCD00003781        GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
NM_001033522.1      GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
NM_001324.2         GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
NM_001033521.1      GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT
                    ************************************************************

HsCD00003781        TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
NM_001033522.1      TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
NM_001324.2         TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
NM_001033521.1      TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT
                    ************************************************************

HsCD00003781        GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
NM_001033522.1      GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
NM_001324.2         GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
NM_001033521.1      GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA
                    ************************************************************

HsCD00003781        CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
NM_001033522.1      CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
NM_001324.2         CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
NM_001033521.1      CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT
                    ************************************************************

HsCD00003781        CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
NM_001033522.1      CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
NM_001324.2         CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
NM_001033521.1      CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA
                    ************************************************************

HsCD00003781        CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
NM_001033522.1      CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
NM_001324.2         CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
NM_001033521.1      CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA
                    ************************************************************

HsCD00003781        AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
NM_001033522.1      AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
NM_001324.2         AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
NM_001033521.1      AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT
                    ************************************************************

HsCD00003781        GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
NM_001033522.1      GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
NM_001324.2         GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
NM_001033521.1      GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC
                    ************************************************************

HsCD00003781        ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
NM_001033522.1      ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
NM_001324.2         ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
NM_001033521.1      ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA
                    ************************************************************

HsCD00003781        CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
NM_001033522.1      CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
NM_001324.2         CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
NM_001033521.1      CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG
                    ************************************************************

HsCD00003781        TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
NM_001033522.1      TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
NM_001324.2         TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
NM_001033521.1      TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC
                    ************************************************************

HsCD00003781        TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
NM_001033522.1      TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
NM_001324.2         TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
NM_001033521.1      TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA
                    ************************************************************

HsCD00003781        CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
NM_001033522.1      CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
NM_001324.2         CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
NM_001033521.1      CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA
                    ************************************************************

HsCD00003781        GCGCGGTTTTGGTACCGGAGATCGACCACTGACTTG
NM_001033522.1      GCGCGGTTTTGGTACCGGAGATCGACCACTGACTGA
NM_001324.2         GCGCGGTTTTGGTACCGGAGATCGACCACTGACTGA
NM_001033521.1      GCGCGGTTTTGGTACCGGAGATCGACCACTGACTGA
                    ********************************** .

Pathways

NCI : BARD1 signaling events
Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of Intronless pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : Processing of Capped Intronless Pre-mRNA
Reactome : Processing of Intronless Pre-mRNAs
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : WD40 : WD40 repeats
UniProt : Q5QPD8
UniProt : Q05048
HPRD : 02653

Clone

Cloning Information : 15272
HIP Master Clone ID : 29471
Original Clone ID : FLH015272.01L

Organism


TAX_ID : 9606
Species Specific ID: 1477

Genome

Chromosome : 20
Map Location : 20q13.2
Ensembl : ENSG00000101138

Reagents

Labome : CSTF1-antibody