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SRPK1 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SRPK1
Gene Name:  SRSF protein kinase 1
Sequence             
Original Clone ID: FLH164608.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00037934

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1968nts         Open reading frame : 1 to 1968
Reference Sequence Alignment

ATGGAGCGGAAAGTGCTTGCGCTCCAGGCCCGAAAGAAAAGGACCAAGGCCAAGAAGGAC
AAAGCCCAAAGGAAATCTGAAACTCAGCACCGAGGCTCTGCTCCCCACTCTGAGAGTGAT
CTACCAGAGCAGGAAGAGGAGATTCTGGGATCTGATGATGATGAGCAAGAAGATCCTAAT
GATTATTGTAAAGGAGGTTATCATCTTGTGAAAATTGGAGATCTATTCAATGGGAGATAC
CATGTGATCCGAAAGTTAGGCTGGGGACACTTTTCAACAGTATGGTTATCATGGGATATT
CAGGGGAAGAAATTTGTGGCAATGAAAGTAGTTAAAAGTGCTGAACATTACACTGAAACA
GCACTAGATGAAATCCGGTTGCTGAAGTCAGTTCGCAATTCAGACCCTAATGATCCAAAT
AGAGAAATGGTTGTTCAACTACTAGATGACTTTAAAATATCAGGAGTTAATGGAACACAT
ATCTGCATGGTATTTGAAGTTTTGGGGCATCATCTGCTCAAGTGGATCATCAAATCCAAT
TATCAGGGGCTTCCACTGCCTTGTGTCAAAAAAATTATTCAGCAAGTGTTACAGGGTCTT
GATTATTTACATACCAAGTGCCGTATCACCCACACTGACATTAAACCAGAGAACATCTTA
TTGTCAGTGAATGAGCAGTACATTCGGAGGCTGGCTGCAGAAGCAACAGAATGGCAGCGA
TCTGGAGCTCCTCCGCCTTCCGGATCTGCAGTCAGTACTGCTCCCCAGCCTAAACCAGCT
GACAAAATGTCAAAGAATAAGAAGAAGAAATTGAAGAAGAAGCAGAAGCGCCAGGCAGAA
TTACTAGAGAAGCGAATGCAGGAAATTGAGGAAATGGAGAAAGAGTCGGGCCCTGGGCAA
AAAAGACCAAACAAGCAAGAAGAATCAGAGAGTCCTGTTGAAAGACCCTTGAAAGAGAAC
CCACCTAATAAAATGACCCAAGAAAAACTTGAAGAGTCAAGTACCATTGGCCAGGATCAA
ACGCTTATGGAACGTGATACAGAGGGTGGTGCAGCAGAAATTAATTGCAATGGAGTGATT
GAAGTCATTAATTATACTCAGAACAGTAATAATGAAACATTGAGACATAAAGAGGATCTA
CATAATGCTAATGACTGTGATGTCCAAAATTTGAATCAGGAATCTAGTTTCCTAAGCTCC
CAAAATGGAGACAGCAGCACATCTCAAGAAACAGACTCTTGTACACCTATAACATCTGAG
GTGTCAGACACCATGGTGTGCCAGTCTTCCTCAACTGTAGGTCAGTCATTCAGTGAACAA
CACATTAGCCAACTTCAAGAAAGCATTCGGGCAGAGATACCCTGTGAAGATGAACAAGAG
CAAGAACATAACGGACCACTGGACAACAAAGGAAAATCCACGGCTGGAAATTTTCTTGTT
AATCCCCTTGAGCCAAAAAATGCAGAAAAGCTCAAGGTGAAGATTGCTGACCTTGGAAAT
GCTTGTTGGGTGCACAAACATTTCACTGAAGATATTCAAACAAGGCAATATCGTTCCTTG
GAAGTTCTAATCGGATCTGGCTATAATACCCCTGCTGACATTTGGAGCACGGCATGCATG
GCCTTTGAACTGGCCACAGGTGACTATTTGTTTGAACCTCATTCAGGGGAAGAGTACACT
CGAGATGAAGATCACATTGCATTGATCATAGAACTTCTGGGGAAGGTGCCTCGCAAGCTC
ATTGTGGCAGGAAAATATTCCAAGGAATTTTTCACCAAAAAAGGTGACCTGAAACATATC
ACGAAGCTGAAACCTTGGGGCCTTTTTGAGGTTCTAGTGGAGAAGTATGAGTGGTCGCAG
GAAGAGGCAGCTGGCTTCACAGATTTCTTACTGCCCATGTTGGAGCTGATCCCTGAGAAG
AGAGCCACTGCCGCCGAGTGTCTCCGGCACCCTTGGCTTAACTCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1974
Start on reference sequence 10
Mutations:
None
Discrepancy:
del@1509,3
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRPK1
Symbol Nomenclature : SRPK1
SYNONYM : SFRSK1
Designation : SFRS protein kinase 1|SR-protein-specific kinase 1|serine/arginine-rich splicing factor kinase 1|serine/threonine-protein kinase SRPK1
Full Nomenclature : SRSF protein kinase 1
GENEID : 6732
Locus Tag : RP3-422H11.1
HGNC : 11305
MIM : 601939
Vega : OTTHUMG00000014583
Target GenBank: NM_003137

 

Reference Sequence Alignment

  

HsCD00037934      ATGGAGCGGAAAGTGCTTGCGCTCCAGGCCCGAAAGAAAAGGACCAAGGCCAAGAAGGAC
NM_003137.4       ATGGAGCGGAAAGTGCTTGCGCTCCAGGCCCGAAAGAAAAGGACCAAGGCCAAGAAGGAC
                  ************************************************************

HsCD00037934      AAAGCCCAAAGGAAATCTGAAACTCAGCACCGAGGCTCTGCTCCCCACTCTGAGAGTGAT
NM_003137.4       AAAGCCCAAAGGAAATCTGAAACTCAGCACCGAGGCTCTGCTCCCCACTCTGAGAGTGAT
                  ************************************************************

HsCD00037934      CTACCAGAGCAGGAAGAGGAGATTCTGGGATCTGATGATGATGAGCAAGAAGATCCTAAT
NM_003137.4       CTACCAGAGCAGGAAGAGGAGATTCTGGGATCTGATGATGATGAGCAAGAAGATCCTAAT
                  ************************************************************

HsCD00037934      GATTATTGTAAAGGAGGTTATCATCTTGTGAAAATTGGAGATCTATTCAATGGGAGATAC
NM_003137.4       GATTATTGTAAAGGAGGTTATCATCTTGTGAAAATTGGAGATCTATTCAATGGGAGATAC
                  ************************************************************

HsCD00037934      CATGTGATCCGAAAGTTAGGCTGGGGACACTTTTCAACAGTATGGTTATCATGGGATATT
NM_003137.4       CATGTGATCCGAAAGTTAGGCTGGGGACACTTTTCAACAGTATGGTTATCATGGGATATT
                  ************************************************************

HsCD00037934      CAGGGGAAGAAATTTGTGGCAATGAAAGTAGTTAAAAGTGCTGAACATTACACTGAAACA
NM_003137.4       CAGGGGAAGAAATTTGTGGCAATGAAAGTAGTTAAAAGTGCTGAACATTACACTGAAACA
                  ************************************************************

HsCD00037934      GCACTAGATGAAATCCGGTTGCTGAAGTCAGTTCGCAATTCAGACCCTAATGATCCAAAT
NM_003137.4       GCACTAGATGAAATCCGGTTGCTGAAGTCAGTTCGCAATTCAGACCCTAATGATCCAAAT
                  ************************************************************

HsCD00037934      AGAGAAATGGTTGTTCAACTACTAGATGACTTTAAAATATCAGGAGTTAATGGAACACAT
NM_003137.4       AGAGAAATGGTTGTTCAACTACTAGATGACTTTAAAATATCAGGAGTTAATGGAACACAT
                  ************************************************************

HsCD00037934      ATCTGCATGGTATTTGAAGTTTTGGGGCATCATCTGCTCAAGTGGATCATCAAATCCAAT
NM_003137.4       ATCTGCATGGTATTTGAAGTTTTGGGGCATCATCTGCTCAAGTGGATCATCAAATCCAAT
                  ************************************************************

HsCD00037934      TATCAGGGGCTTCCACTGCCTTGTGTCAAAAAAATTATTCAGCAAGTGTTACAGGGTCTT
NM_003137.4       TATCAGGGGCTTCCACTGCCTTGTGTCAAAAAAATTATTCAGCAAGTGTTACAGGGTCTT
                  ************************************************************

HsCD00037934      GATTATTTACATACCAAGTGCCGTATCACCCACACTGACATTAAACCAGAGAACATCTTA
NM_003137.4       GATTATTTACATACCAAGTGCCGTATCATCCACACTGACATTAAACCAGAGAACATCTTA
                  **************************** *******************************

HsCD00037934      TTGTCAGTGAATGAGCAGTACATTCGGAGGCTGGCTGCAGAAGCAACAGAATGGCAGCGA
NM_003137.4       TTGTCAGTGAATGAGCAGTACATTCGGAGGCTGGCTGCAGAAGCAACAGAATGGCAGCGA
                  ************************************************************

HsCD00037934      TCTGGAGCTCCTCCGCCTTCCGGATCTGCAGTCAGTACTGCTCCCCAGCCTAAACCAGCT
NM_003137.4       TCTGGAGCTCCTCCGCCTTCCGGATCTGCAGTCAGTACTGCTCCCCAGCCTAAACCAGCT
                  ************************************************************

HsCD00037934      GACAAAATGTCAAAGAATAAGAAGAAGAAATTGAAGAAGAAGCAGAAGCGCCAGGCAGAA
NM_003137.4       GACAAAATGTCAAAGAATAAGAAGAAGAAATTGAAGAAGAAGCAGAAGCGCCAGGCAGAA
                  ************************************************************

HsCD00037934      TTACTAGAGAAGCGAATGCAGGAAATTGAGGAAATGGAGAAAGAGTCGGGCCCTGGGCAA
NM_003137.4       TTACTAGAGAAGCGAATGCAGGAAATTGAGGAAATGGAGAAAGAGTCGGGCCCTGGGCAA
                  ************************************************************

HsCD00037934      AAAAGACCAAACAAGCAAGAAGAATCAGAGAGTCCTGTTGAAAGACCCTTGAAAGAGAAC
NM_003137.4       AAAAGACCAAACAAGCAAGAAGAATCAGAGAGTCCTGTTGAAAGACCCTTGAAAGAGAAC
                  ************************************************************

HsCD00037934      CCACCTAATAAAATGACCCAAGAAAAACTTGAAGAGTCAAGTACCATTGGCCAGGATCAA
NM_003137.4       CCACCTAATAAAATGACCCAAGAAAAACTTGAAGAGTCAAGTACCATTGGCCAGGATCAA
                  ************************************************************

HsCD00037934      ACGCTTATGGAACGTGATACAGAGGGTGGTGCAGCAGAAATTAATTGCAATGGAGTGATT
NM_003137.4       ACGCTTATGGAACGTGATACAGAGGGTGGTGCAGCAGAAATTAATTGCAATGGAGTGATT
                  ************************************************************

HsCD00037934      GAAGTCATTAATTATACTCAGAACAGTAATAATGAAACATTGAGACATAAAGAGGATCTA
NM_003137.4       GAAGTCATTAATTATACTCAGAACAGTAATAATGAAACATTGAGACATAAAGAGGATCTA
                  ************************************************************

HsCD00037934      CATAATGCTAATGACTGTGATGTCCAAAATTTGAATCAGGAATCTAGTTTCCTAAGCTCC
NM_003137.4       CATAATGCTAATGACTGTGATGTCCAAAATTTGAATCAGGAATCTAGTTTCCTAAGCTCC
                  ************************************************************

HsCD00037934      CAAAATGGAGACAGCAGCACATCTCAAGAAACAGACTCTTGTACACCTATAACATCTGAG
NM_003137.4       CAAAATGGAGACAGCAGCACATCTCAAGAAACAGACTCTTGTACACCTATAACATCTGAG
                  ************************************************************

HsCD00037934      GTGTCAGACACCATGGTGTGCCAGTCTTCCTCAACTGTAGGTCAGTCATTCAGTGAACAA
NM_003137.4       GTGTCAGACACCATGGTGTGCCAGTCTTCCTCAACTGTAGGTCAGTCATTCAGTGAACAA
                  ************************************************************

HsCD00037934      CACATTAGCCAACTTCAAGAAAGCATTCGGGCAGAGATACCCTGTGAAGATGAACAAGAG
NM_003137.4       CACATTAGCCAACTTCAAGAAAGCATTCGGGCAGAGATACCCTGTGAAGATGAACAAGAG
                  ************************************************************

HsCD00037934      CAAGAACATAACGGACCACTGGACAACAAAGGAAAATCCACGGCTGGAAATTTTCTTGTT
NM_003137.4       CAAGAACATAACGGACCACTGGACAACAAAGGAAAATCCACGGCTGGAAATTTTCTTGTT
                  ************************************************************

HsCD00037934      AATCCCCTTGAGCCAAAAAATGCAGAAAAGCTCAAGGTGAAGATTGCTGACCTTGGAAAT
NM_003137.4       AATCCCCTTGAGCCAAAAAATGCAGAAAAGCTCAAGGTGAAGATTGCTGACCTTGGAAAT
                  ************************************************************

HsCD00037934      GCTTGTTGGGTGCACAAACATTTCACTGAAGATATTCAAACAAGGCAATATCGTTCCTTG
NM_003137.4       GCTTGTTGGGTGCACAAACATTTCACTGAAGATATTCAAACAAGGCAATATCGTTCCTTG
                  ************************************************************

HsCD00037934      GAAGTTCTAATCGGATCTGGCTATAATACCCCTGCTGACATTTGGAGCACGGCATGCATG
NM_003137.4       GAAGTTCTAATCGGATCTGGCTATAATACCCCTGCTGACATTTGGAGCACGGCATGCATG
                  ************************************************************

HsCD00037934      GCCTTTGAACTGGCCACAGGTGACTATTTGTTTGAACCTCATTCAGGGGAAGAGTACACT
NM_003137.4       GCCTTTGAACTGGCCACAGGTGACTATTTGTTTGAACCTCATTCAGGGGAAGAGTACACT
                  ************************************************************

HsCD00037934      CGAGATGAAGATCACATTGCATTGATCATAGAACTTCTGGGGAAGGTGCCTCGCAAGCTC
NM_003137.4       CGAGATGAAGATCACATTGCATTGATCATAGAACTTCTGGGGAAGGTGCCTCGCAAGCTC
                  ************************************************************

HsCD00037934      ATTGTGGCAGGAAAATATTCCAAGGAATTTTTCACCAAAAAAGGTGACCTGAAACATATC
NM_003137.4       ATTGTGGCAGGAAAATATTCCAAGGAATTTTTCACCAAAAAAGGTGACCTGAAACATATC
                  ************************************************************

HsCD00037934      ACGAAGCTGAAACCTTGGGGCCTTTTTGAGGTTCTAGTGGAGAAGTATGAGTGGTCGCAG
NM_003137.4       ACGAAGCTGAAACCTTGGGGCCTTTTTGAGGTTCTAGTGGAGAAGTATGAGTGGTCGCAG
                  ************************************************************

HsCD00037934      GAAGAGGCAGCTGGCTTCACAGATTTCTTACTGCCCATGTTGGAGCTGATCCCTGAGAAG
NM_003137.4       GAAGAGGCAGCTGGCTTCACAGATTTCTTACTGCCCATGTTGGAGCTGATCCCTGAGAAG
                  ************************************************************

HsCD00037934      AGAGCCACTGCCGCCGAGTGTCTCCGGCACCCTTGGCTTAACTCCTTG
NM_003137.4       AGAGCCACTGCCGCCGAGTGTCTCCGGCACCCTTGGCTTAACTCCTAA
                  **********************************************:.

Pathways

WikiPathway : mRNA processing

Protein

SMART domain : TyrKc : Tyrosine kinase, catalytic domain
SMART domain : S_TKc : Serine/Threonine protein kinases, catalytic domain
UniProt : B4DS61
UniProt : Q96SB4
HPRD : 15992

Clone

Cloning Information : 164608
HIP Master Clone ID : 124241
Original Clone ID : FLH164608.01L

Organism


TAX_ID : 9606
Species Specific ID: 6732

Genome

Chromosome : 6
Map Location : 6p21.31
Ensembl : ENSG00000096063

Reagents

Labome : SRPK1-antibody