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IRAK4 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  IRAK4
Gene Name:  interleukin-1 receptor-associated kinase 4
Sequence             
Original Clone ID: FLH164615.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00037941

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1383nts         Open reading frame : 1 to 1383
Reference Sequence Alignment

ATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAATTAGG
AAGCTGTCAGATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTAGCTGTAGCTATTAAA
AAACCATCTGGTGATGATAGATACAATCAGTTTCACATAAGGAGATTTGAAGCATTACTT
CAAACTGGAAAAAGTCCCACTTCTGAATTACTGTTTGACTGGGGCACCACAAATTGCACA
GTTGGTGATCTTGTGGATCTTTTGATCCAAAATGAATTTTTTGCTCCTGCAAGTCTTTTG
CTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAAAGAAGCTATAACAGTT
CAGCAAAAACAGATGCCTTTCTGTGACAAAGACAGGACATTGATGACACCTGTGCAGAAT
CTTGAACAAAGCTATATGCCACCTGACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTT
AGTGATACACGTTTTCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTT
GATGAACGACCCATTTCTGTTGGTGGTAATAAAATGGGAGAGGGAGGATTTGGAGTTGTA
TATAAAGGCTACGTAAATAACACAACTGTGGCAGTGAAGAAGCTTGCAGCAATGGTTGAC
ATTACTACTGAAGAACTGAAACAGCAGTTTGATCAAGAAATAAAAGTAATGGCAAAGTGT
CAACATGAAAACTTAGTAGAACTACTTGGTTTCTCAAGTGATGGAGATGACCTCTGCTTA
GTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACT
CCACCACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGCATCAAT
TTTCTACATGAAAATCATCATATTCATAGAGATATTAAAAGTGCAAATATCTTACTGGAT
GAAGCTTTTACTGCTAAAATATCTGACTTTGGCCTTGCACGGGCTTCTGAGAAGTTTGCC
CAGACAGTCATGACTAGCAGAATTGTGGGAACAACAGCTTATATGGCACCAGAAGCTTTG
CGTGGAGAAATAACACCCAAATCTGATATTTACAGCTTTGGTGTGGTTTTACTAGAAATA
ATAACTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCTAGATATTAAA
GAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGATAAAAAGATGAATGAT
GCTGATTCCACTTCAGTTGAAGCTATGTACTCTGTTGCTAGTCAATGTCTGCATGAAAAG
AAAAATAAGAGACCAGACATTAAGAAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCT
TTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1432
Start on reference sequence 50
Mutations:
None
Discrepancy:
g291a; g1295t,G432V;
 c1302t; g1310t,R437L;
 c1332a,S444R; c1353a,H451Q
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IRAK4
Symbol Nomenclature : IRAK4
SYNONYM : IPD1; IRAK-4; NY-REN-64; REN64
Designation : renal carcinoma antigen NY-REN-64
Full Nomenclature : interleukin-1 receptor-associated kinase 4
GENEID : 51135
HGNC : 17967
MIM : 606883
Target GenBank: NM_016123

 

Reference Sequence Alignment

  

HsCD00037941        ATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAATTAGG
NM_001114182.2      ATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAATTAGG
NM_016123.3         ATGAACAAACCCATAACACCATCAACATATGTGCGCTGCCTCAATGTTGGACTAATTAGG
                    ************************************************************

HsCD00037941        AAGCTGTCAGATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTAGCTGTAGCTATTAAA
NM_001114182.2      AAGCTGTCAGATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTAGCTGTAGCTATTAAA
NM_016123.3         AAGCTGTCAGATTTTATTGATCCTCAAGAAGGATGGAAGAAGTTAGCTGTAGCTATTAAA
                    ************************************************************

HsCD00037941        AAACCATCTGGTGATGATAGATACAATCAGTTTCACATAAGGAGATTTGAAGCATTACTT
NM_001114182.2      AAACCATCTGGTGATGATAGATACAATCAGTTTCACATAAGGAGATTTGAAGCATTACTT
NM_016123.3         AAACCATCTGGTGATGATAGATACAATCAGTTTCACATAAGGAGATTTGAAGCATTACTT
                    ************************************************************

HsCD00037941        CAAACTGGAAAAAGTCCCACTTCTGAATTACTGTTTGACTGGGGCACCACAAATTGCACA
NM_001114182.2      CAAACTGGAAAAAGTCCCACTTCTGAATTACTGTTTGACTGGGGCACCACAAATTGCACA
NM_016123.3         CAAACTGGAAAAAGTCCCACTTCTGAATTACTGTTTGACTGGGGCACCACAAATTGCACA
                    ************************************************************

HsCD00037941        GTTGGTGATCTTGTGGATCTTTTGATCCAAAATGAATTTTTTGCTCCTGCAAGTCTTTTG
NM_001114182.2      GTTGGTGATCTTGTGGATCTTTTGATCCAAAATGAATTTTTTGCTCCTGCGAGTCTTTTG
NM_016123.3         GTTGGTGATCTTGTGGATCTTTTGATCCAAAATGAATTTTTTGCTCCTGCGAGTCTTTTG
                    **************************************************.*********

HsCD00037941        CTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAAAGAAGCTATAACAGTT
NM_001114182.2      CTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAAAGAAGCTATAACAGTT
NM_016123.3         CTCCCAGATGCTGTTCCCAAAACTGCTAATACACTACCTTCTAAAGAAGCTATAACAGTT
                    ************************************************************

HsCD00037941        CAGCAAAAACAGATGCCTTTCTGTGACAAAGACAGGACATTGATGACACCTGTGCAGAAT
NM_001114182.2      CAGCAAAAACAGATGCCTTTCTGTGACAAAGACAGGACATTGATGACACCTGTGCAGAAT
NM_016123.3         CAGCAAAAACAGATGCCTTTCTGTGACAAAGACAGGACATTGATGACACCTGTGCAGAAT
                    ************************************************************

HsCD00037941        CTTGAACAAAGCTATATGCCACCTGACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTT
NM_001114182.2      CTTGAACAAAGCTATATGCCACCTGACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTT
NM_016123.3         CTTGAACAAAGCTATATGCCACCTGACTCCTCAAGTCCAGAAAATAAAAGTTTAGAAGTT
                    ************************************************************

HsCD00037941        AGTGATACACGTTTTCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTT
NM_001114182.2      AGTGATACACGTTTTCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTT
NM_016123.3         AGTGATACACGTTTTCACAGTTTTTCATTTTATGAATTGAAGAATGTCACAAATAACTTT
                    ************************************************************

HsCD00037941        GATGAACGACCCATTTCTGTTGGTGGTAATAAAATGGGAGAGGGAGGATTTGGAGTTGTA
NM_001114182.2      GATGAACGACCCATTTCTGTTGGTGGTAATAAAATGGGAGAGGGAGGATTTGGAGTTGTA
NM_016123.3         GATGAACGACCCATTTCTGTTGGTGGTAATAAAATGGGAGAGGGAGGATTTGGAGTTGTA
                    ************************************************************

HsCD00037941        TATAAAGGCTACGTAAATAACACAACTGTGGCAGTGAAGAAGCTTGCAGCAATGGTTGAC
NM_001114182.2      TATAAAGGCTACGTAAATAACACAACTGTGGCAGTGAAGAAGCTTGCAGCAATGGTTGAC
NM_016123.3         TATAAAGGCTACGTAAATAACACAACTGTGGCAGTGAAGAAGCTTGCAGCAATGGTTGAC
                    ************************************************************

HsCD00037941        ATTACTACTGAAGAACTGAAACAGCAGTTTGATCAAGAAATAAAAGTAATGGCAAAGTGT
NM_001114182.2      ATTACTACTGAAGAACTGAAACAGCAGTTTGATCAAGAAATAAAAGTAATGGCAAAGTGT
NM_016123.3         ATTACTACTGAAGAACTGAAACAGCAGTTTGATCAAGAAATAAAAGTAATGGCAAAGTGT
                    ************************************************************

HsCD00037941        CAACATGAAAACTTAGTAGAACTACTTGGTTTCTCAAGTGATGGAGATGACCTCTGCTTA
NM_001114182.2      CAACATGAAAACTTAGTAGAACTACTTGGTTTCTCAAGTGATGGAGATGACCTCTGCTTA
NM_016123.3         CAACATGAAAACTTAGTAGAACTACTTGGTTTCTCAAGTGATGGAGATGACCTCTGCTTA
                    ************************************************************

HsCD00037941        GTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACT
NM_001114182.2      GTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACT
NM_016123.3         GTATATGTTTACATGCCTAATGGTTCATTGCTAGACAGACTCTCTTGCTTGGATGGTACT
                    ************************************************************

HsCD00037941        CCACCACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGCATCAAT
NM_001114182.2      CCACCACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGCATCAAT
NM_016123.3         CCACCACTTTCTTGGCACATGAGATGCAAGATTGCTCAGGGTGCAGCTAATGGCATCAAT
                    ************************************************************

HsCD00037941        TTTCTACATGAAAATCATCATATTCATAGAGATATTAAAAGTGCAAATATCTTACTGGAT
NM_001114182.2      TTTCTACATGAAAATCATCATATTCATAGAGATATTAAAAGTGCAAATATCTTACTGGAT
NM_016123.3         TTTCTACATGAAAATCATCATATTCATAGAGATATTAAAAGTGCAAATATCTTACTGGAT
                    ************************************************************

HsCD00037941        GAAGCTTTTACTGCTAAAATATCTGACTTTGGCCTTGCACGGGCTTCTGAGAAGTTTGCC
NM_001114182.2      GAAGCTTTTACTGCTAAAATATCTGACTTTGGCCTTGCACGGGCTTCTGAGAAGTTTGCC
NM_016123.3         GAAGCTTTTACTGCTAAAATATCTGACTTTGGCCTTGCACGGGCTTCTGAGAAGTTTGCC
                    ************************************************************

HsCD00037941        CAGACAGTCATGACTAGCAGAATTGTGGGAACAACAGCTTATATGGCACCAGAAGCTTTG
NM_001114182.2      CAGACAGTCATGACTAGCAGAATTGTGGGAACAACAGCTTATATGGCACCAGAAGCTTTG
NM_016123.3         CAGACAGTCATGACTAGCAGAATTGTGGGAACAACAGCTTATATGGCACCAGAAGCTTTG
                    ************************************************************

HsCD00037941        CGTGGAGAAATAACACCCAAATCTGATATTTACAGCTTTGGTGTGGTTTTACTAGAAATA
NM_001114182.2      CGTGGAGAAATAACACCCAAATCTGATATTTACAGCTTTGGTGTGGTTTTACTAGAAATA
NM_016123.3         CGTGGAGAAATAACACCCAAATCTGATATTTACAGCTTTGGTGTGGTTTTACTAGAAATA
                    ************************************************************

HsCD00037941        ATAACTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCTAGATATTAAA
NM_001114182.2      ATAACTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCTAGATATTAAA
NM_016123.3         ATAACTGGACTTCCAGCTGTGGATGAACACCGTGAACCTCAGTTATTGCTAGATATTAAA
                    ************************************************************

HsCD00037941        GAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGATAAAAAGATGAATGAT
NM_001114182.2      GAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGATAAAAAGATGAATGAT
NM_016123.3         GAAGAAATTGAAGATGAAGAAAAGACAATTGAAGATTATATTGATAAAAAGATGAATGAT
                    ************************************************************

HsCD00037941        GCTGATTCCACTTCAGTTGAAGCTATGTACTCTGTTGCTAGTCAATGTCTGCATGAAAAG
NM_001114182.2      GCTGATTCCACTTCAGTTGAAGCTATGTACTCTGTTGCTAGTCAATGTCTGCATGAAAAG
NM_016123.3         GCTGATTCCACTTCAGTTGAAGCTATGTACTCTGTTGCTAGTCAATGTCTGCATGAAAAG
                    ************************************************************

HsCD00037941        AAAAATAAGAGACCAGACATTAAGAAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCT
NM_001114182.2      AAAAATAAGAGACCAGACATTAAGAAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCT
NM_016123.3         AAAAATAAGAGACCAGACATTAAGAAGGTTCAACAGCTGCTGCAAGAGATGACAGCTTCT
                    ************************************************************

HsCD00037941        TTG
NM_001114182.2      TAA
NM_016123.3         TAA
                    *:.

Pathways

NCI : Endogenous TLR signaling
NCI : IL1-mediated signaling events
Panther : Toll receptor signaling pathway
Reactome : Activated TLR4 signalling
Reactome : Cytokine Signaling in Immune system
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Interleukin-1 signaling
Reactome : MyD88 cascade initiated on plasma membrane
Reactome : MyD88 dependent cascade initiated on endosome
Reactome : MyD88:Mal cascade initiated on plasma membrane
Reactome : Signaling by Interleukins
Reactome : TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling
Reactome : TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome : Toll Like Receptor 10 (TLR10) Cascade
Reactome : Toll Like Receptor 2 (TLR2) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll Like Receptor 5 (TLR5) Cascade
Reactome : Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome : Toll Like Receptor 9 (TLR9) Cascade
Reactome : Toll Like Receptor TLR1:TLR2 Cascade
Reactome : Toll Like Receptor TLR6:TLR2 Cascade
Reactome : Toll-Like Receptors Cascades
WikiPathway : IL-1 signaling pathway
WikiPathway : Regulation of toll-like receptor signaling pathway
WikiPathway : Toll-like receptor signaling pathway

Protein

SMART domain : S_TKc : Serine/Threonine protein kinases, catalytic domain
SMART domain : TyrKc : Tyrosine kinase, catalytic domain
UniProt : Q69FE3
UniProt : B4E359
UniProt : Q9NWZ3
UniProt : B2RAP9
HPRD : 06051

Clone

Cloning Information : 164615
HIP Master Clone ID : 124251
Original Clone ID : FLH164615.01L

Organism


TAX_ID : 9606
Species Specific ID: 51135

Genome

Chromosome : 12
Map Location : 12q12
Ensembl : ENSG00000198001

Reagents

Labome : IRAK4-antibody