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CNK (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PLK3
Gene Name:  polo-like kinase 3
Sequence             
Original Clone ID: FLH164616.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00037942

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1824nts         Open reading frame : 1 to 1824
Reference Sequence Alignment

ATGCTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCTCATCACGGACCCGCGCAGCGGC
CGCACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTACGAGGCC
ACTGACACAGAGACTGGCAGCGCCTACGCTGTCAAAGTCATCCCGCAGAGCCGCGTCGCC
AAGCCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCTGCAGCAC
CGCCACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATTTTCTTG
GAGCTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGGCACACCCTGTTGGAG
CCAGAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGC
GGCATCTTGCACCGGGACCTCAAGTTGGGAAATTTTTTCATCACTGAGAACATGGAACTG
AAGGTGGGGGATTTTGGGCTGGCAGCCCGGTTGGAGCCTCCGGAGCAGAGGAAGAAGACC
ATCTGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCTGCTGAGACAGGGCCACGGCCCT
GAGGCGGATGTATGGTCACTGGGCTGTGTCATGTACACGCTGCTCTGCGGGAGCCCTCCC
TTTGAGACGGCTGACCTGAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTG
CCTGCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCACCC
CGAGACCGCCCCTCTATTGACCAGATCCTGCGCCATGACTTCTTTACCAAGGGCTACACC
CCCGATCGACTCCCTATCAGCAGCTGCGTGACAGTCCCAGACCTGACACCCCCCAACCCA
GCTAGGAGTCTGTTTGCCAAAGTTACCAAGAGCCTCTTTGGCAGAAAGAAGAAGAGTAAG
AATCATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGTGAGCGGCCTCATGCGCACATCC
GTTGGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCCTGTCAGC
CTGGTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGCAAGCAGTGGAGAT
GGATTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGAGTCAGCCCTTTGTGCTCTGAGA
AATTGTATAGCCTTCATGCCCCCAGCGGAACAGAACCCGGCCCCCCTGGCCCAGCCAGAG
CCTCTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAA
CTGTCCAGCCGCCGTGTGGCTGTGCTCTTCAACGATGGCACACATATGGCCCTGTCGGCC
AACAGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACTTCTCCTTCTCCGTGGGT
GCTGTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCTGCGGTACTTCGCCTCCTACATG
GAGCAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCT
CCGCCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGAT
GGCACTGTCCAGGTGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGGGAG
CCCCTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGCTTGTACTTACCTCGCTTCCCAC
CTTCGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGCGACTCCGCTATGCTCTGCGCCTG
CTCCGGGACCGCAGCCCAGCTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1860
Start on reference sequence 37
Mutations:
None
Discrepancy:
a603g; t1212c
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PLK3
Symbol Nomenclature : PLK3
SYNONYM : CNK; FNK; PRK
Designation : FGF-inducible kinase|PLK-3|cytokine-inducible kinase|cytokine-inducible serine/threonine-protein kinase|proliferation-related kinase|serine/threonine-protein kinase PLK3
Full Nomenclature : polo-like kinase 3
GENEID : 1263
Locus Tag : RP11-269F19.6
HGNC : 2154
MIM : 602913
Vega : OTTHUMG00000008491
Target GenBank: NM_004073

 

Reference Sequence Alignment

  

HsCD00037942      ------------------------------------------------------------
NM_004073.2       ATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGCCGCCGCGCCC
                                                                              

HsCD00037942      ---------------------------------------------------------ATG
NM_004073.2       GCTCCCCCGGCCGGGCCCGGGCCGCCTCCGAGTGCCTTGCGCGGACCTGAGCTGGAGATG
                                                                           ***

HsCD00037942      CTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCTCATCACGGACCCGCGCAGCGGCCGC
NM_004073.2       CTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCTCATCACGGACCCGCGCAGCGGCCGC
                  ************************************************************

HsCD00037942      ACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTACGAGGCCACT
NM_004073.2       ACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTACGAGGCCACT
                  ************************************************************

HsCD00037942      GACACAGAGACTGGCAGCGCCTACGCTGTCAAAGTCATCCCGCAGAGCCGCGTCGCCAAG
NM_004073.2       GACACAGAGACTGGCAGCGCCTACGCTGTCAAAGTCATCCCGCAGAGCCGCGTCGCCAAG
                  ************************************************************

HsCD00037942      CCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCTGCAGCACCGC
NM_004073.2       CCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCTGCAGCACCGC
                  ************************************************************

HsCD00037942      CACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATTTTCTTGGAG
NM_004073.2       CACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATTTTCTTGGAG
                  ************************************************************

HsCD00037942      CTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGGCACACCCTGTTGGAGCCA
NM_004073.2       CTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGGCACACCCTGTTGGAGCCA
                  ************************************************************

HsCD00037942      GAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGCGGC
NM_004073.2       GAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGCGGC
                  ************************************************************

HsCD00037942      ATCTTGCACCGGGACCTCAAGTTGGGAAATTTTTTCATCACTGAGAACATGGAACTGAAG
NM_004073.2       ATCTTGCACCGGGACCTCAAGTTGGGAAATTTTTTCATCACTGAGAACATGGAACTGAAG
                  ************************************************************

HsCD00037942      GTGGGGGATTTTGGGCTGGCAGCCCGGTTGGAGCCTCCGGAGCAGAGGAAGAAGACCATC
NM_004073.2       GTGGGGGATTTTGGGCTGGCAGCCCGGTTGGAGCCTCCGGAGCAGAGGAAGAAGACCATC
                  ************************************************************

HsCD00037942      TGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCTGCTGAGACAGGGCCACGGCCCTGAG
NM_004073.2       TGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCTGCTGAGACAGGGCCACGGCCCTGAG
                  ************************************************************

HsCD00037942      GCGGATGTATGGTCACTGGGCTGTGTCATGTACACGCTGCTCTGCGGGAGCCCTCCCTTT
NM_004073.2       GCGGATGTATGGTCACTGGGCTGTGTCATGTACACGCTGCTCTGCGGGAGCCCTCCCTTT
                  ************************************************************

HsCD00037942      GAGACGGCTGACCTGAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTGCCT
NM_004073.2       GAGACGGCTGACCTGAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTGCCT
                  ************************************************************

HsCD00037942      GCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCACCCCGA
NM_004073.2       GCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCACCCCGA
                  ************************************************************

HsCD00037942      GACCGCCCCTCTATTGACCAGATCCTGCGCCATGACTTCTTTACCAAGGGCTACACCCCC
NM_004073.2       GACCGCCCCTCTATTGACCAGATCCTGCGCCATGACTTCTTTACCAAGGGCTACACCCCC
                  ************************************************************

HsCD00037942      GATCGACTCCCTATCAGCAGCTGCGTGACAGTCCCAGACCTGACACCCCCCAACCCAGCT
NM_004073.2       GATCGACTCCCTATCAGCAGCTGCGTGACAGTCCCAGACCTGACACCCCCCAACCCAGCT
                  ************************************************************

HsCD00037942      AGGAGTCTGTTTGCCAAAGTTACCAAGAGCCTCTTTGGCAGAAAGAAGAAGAGTAAGAAT
NM_004073.2       AGGAGTCTGTTTGCCAAAGTTACCAAGAGCCTCTTTGGCAGAAAGAAGAAGAGTAAGAAT
                  ************************************************************

HsCD00037942      CATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGTGAGCGGCCTCATGCGCACATCCGTT
NM_004073.2       CATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGTGAGCGGCCTCATGCGCACATCCGTT
                  ************************************************************

HsCD00037942      GGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCCTGTCAGCCTG
NM_004073.2       GGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCCTGTCAGCCTG
                  ************************************************************

HsCD00037942      GTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGCAAGCAGTGGAGATGGA
NM_004073.2       GTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGCAAGCAGTGGAGATGGA
                  ************************************************************

HsCD00037942      TTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGAGTCAGCCCTTTGTGCTCTGAGAAAT
NM_004073.2       TTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGAGTCAGCCCTTTGTGCTCTGAGAAAT
                  ************************************************************

HsCD00037942      TGTATAGCCTTCATGCCCCCAGCGGAACAGAACCCGGCCCCCCTGGCCCAGCCAGAGCCT
NM_004073.2       TGTATAGCCTTCATGCCCCCAGCGGAACAGAACCCGGCCCCCCTGGCCCAGCCAGAGCCT
                  ************************************************************

HsCD00037942      CTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAACTG
NM_004073.2       CTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAACTG
                  ************************************************************

HsCD00037942      TCCAGCCGCCGTGTGGCTGTGCTCTTCAACGATGGCACACATATGGCCCTGTCGGCCAAC
NM_004073.2       TCCAGCCGCCGTGTGGCTGTGCTCTTCAACGATGGCACACATATGGCCCTGTCGGCCAAC
                  ************************************************************

HsCD00037942      AGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACTTCTCCTTCTCCGTGGGTGCT
NM_004073.2       AGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACTTCTCCTTCTCCGTGGGTGCT
                  ************************************************************

HsCD00037942      GTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCTGCGGTACTTCGCCTCCTACATGGAG
NM_004073.2       GTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCTGCGGTACTTCGCCTCCTACATGGAG
                  ************************************************************

HsCD00037942      CAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCTCCG
NM_004073.2       CAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCTCCG
                  ************************************************************

HsCD00037942      CCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGATGGC
NM_004073.2       CCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGATGGC
                  ************************************************************

HsCD00037942      ACTGTCCAGGTGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGGGAGCCC
NM_004073.2       ACTGTCCAGGTGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGGGAGCCC
                  ************************************************************

HsCD00037942      CTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGCTTGTACTTACCTCGCTTCCCACCTT
NM_004073.2       CTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGCTTGTACTTACCTCGCTTCCCACCTT
                  ************************************************************

HsCD00037942      CGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGCGACTCCGCTATGCTCTGCGCCTGCTC
NM_004073.2       CGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGCGACTCCGCTATGCTCTGCGCCTGCTC
                  ************************************************************

HsCD00037942      CGGGACCGCAGCCCAGCTTTG
NM_004073.2       CGGGACCGCAGCCCAGCCTAG
                  ***************** *:*

Pathways

NCI : Direct p53 effectors
NCI : PLK3 signaling events
NCI : Polo-like kinase signaling events in the cell cycle
NCI : p73 transcription factor network
WikiPathway : Integrated Breast Cancer Pathway

Clone

Cloning Information : 164616
HIP Master Clone ID : 124252
Original Clone ID : FLH164616.01L

Organism


TAX_ID : 9606
Species Specific ID: 1263

Genome

Chromosome : 1
Map Location : 1p34.1
Ensembl : ENSG00000173846

Reagents

Labome : PLK3-antibody