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HIPK4 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  HIPK4
Gene Name:  homeodomain interacting protein kinase 4
Sequence             
Original Clone ID: FLH164619.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00037945

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1851nts         Open reading frame : 1 to 1851
Reference Sequence Alignment

ATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGG
ACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCAAG
ATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCAC
TGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCAT
GACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG
AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCACCCTGCAGGTG
CTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCCACGCTGATCTCAAGCCTGAG
AACATCATGCTGGTGGACCAGACCCGCTGCCCCTTCAGGGTCAAGGTGATTGACTTCGGA
TCCGCCAGCATTTTCAGCGAGGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTC
TACCGGGCCCCTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC
CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGGCAACAACGAG
TACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGCCCAAGCCACACCTGTTGCAC
GCCGCCTGCAAGGCCCACCACTTCTTCAAGCGCAACCCCCACCCTGACGCTGCCAACCCC
TGGCAGCTCAAGTCCTCGGCTGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGC
CGCAAGTATATGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC
AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCTCAAGAGCATG
GTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACGAACGCATCAGCCCCAGTGCT
GCCCTGCGCCACCCCTTCGTGTCCATGCAGCAGCTGCGCAGTGCCCACGAGACCACCCAC
TACTACCAGCTCTCGCTGCGCAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCC
ACGCCCGTCGTGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG
GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGAGAAGGCACCA
GGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTCTGCAGGAGGCTGGGCATGGG
CTGTGGGGTGAGACCTGCACCAATGCGGTCTCCGACATGATGGTCCCCCTCAAGGCAGCC
ATCACTGGCCACCATGTGCCCGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGC
CGCCTGGCAGGCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC
TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCCCTGCCGGGGC
AGCAGCTGGGAGGAAGGAGAGTATCTCGGGGCCTCTGCTGAGCCACTGGCCATCCTGCAG
CGAGATGAGGATGGGCCCAACATTGACAACATGACCATGGAAGCTGAGAGGCCAGACCCT
GAGCTCTTCGACCCCAGCAGCTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTG
GAGAGCGTCAGGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT
CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCAATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1920
Start on reference sequence 295
Mutations:
None
Discrepancy:
ins@754,225
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HIPK4
Symbol Nomenclature : HIPK4
Designation : homeodomain-interacting protein kinase 4
Full Nomenclature : homeodomain interacting protein kinase 4
GENEID : 147746
HGNC : 19007
MIM : 611712
Vega : OTTHUMG00000182598
Target GenBank: NM_144685

 

Reference Sequence Alignment

  

HsCD00037945      ATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGG
NM_144685.3       ATGTCCACCATCCAGTCGGAGACTGACTGCTACGACATCATCGAGGTCTTGGGCAAGGGG
                  ************************************************************

HsCD00037945      ACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCAAG
NM_144685.3       ACCTTCGGGGAGGTAGCCAAGGGCTGGCGGCGGAGCACGGGCGAGATGGTGGCCATCAAG
                  ************************************************************

HsCD00037945      ATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCAC
NM_144685.3       ATCCTCAAGAATGACGCCTACCGCAACCGCATCATCAAAAACGAGCTGAAGCTGCTGCAC
                  ************************************************************

HsCD00037945      TGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCAT
NM_144685.3       TGCATGCGAGGCCTAGACCCTGAAGAGGCCCACGTCATCCGCTTCCTTGAGTTCTTCCAT
                  ************************************************************

HsCD00037945      GACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG
NM_144685.3       GACGCCCTCAAGTTCTACCTGGTCTTTGAGCTGCTGGAGCAAAACCTTTTCGAGTTCCAG
                  ************************************************************

HsCD00037945      AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCACCCTGCAGGTG
NM_144685.3       AAGGAGAACAACTTCGCGCCCCTCCCCGCCCGCCACATCCGTACAGTCACCCTGCAGGTG
                  ************************************************************

HsCD00037945      CTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCCACGCTGATCTCAAGCCTGAG
NM_144685.3       CTCACAGCCCTGGCCCGGCTCAAGGAGCTGGCTATCATCCACGCTGATCTCAAGCCTGAG
                  ************************************************************

HsCD00037945      AACATCATGCTGGTGGACCAGACCCGCTGCCCCTTCAGGGTCAAGGTGATTGACTTCGGA
NM_144685.3       AACATCATGCTGGTGGACCAGACCCGCTGCCCCTTCAGGGTCAAGGTGATTGACTTCGGA
                  ************************************************************

HsCD00037945      TCCGCCAGCATTTTCAGCGAGGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTC
NM_144685.3       TCCGCCAGCATTTTCAGCGAGGTGCGCTACGTGAAGGAGCCATACATCCAGTCGCGCTTC
                  ************************************************************

HsCD00037945      TACCGGGCCCCTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC
NM_144685.3       TACCGGGCCCCTGAGATCCTGCTGGGGCTGCCCTTCTGCGAGAAGGTGGACGTGTGGTCC
                  ************************************************************

HsCD00037945      CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGGCAACAACGAG
NM_144685.3       CTGGGCTGCGTCATGGCTGAGCTGCACCTGGGCTGGCCTCTCTACCCCGGCAACAACGAG
                  ************************************************************

HsCD00037945      TACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGCCCAAGCCACACCTGTTGCAC
NM_144685.3       TACGACCAGGTGCGCTACATCTGCGAAACCCAGGGCCTGCCCAAGCCACACCTGTTGCAC
                  ************************************************************

HsCD00037945      GCCGCCTGCAAGGCCCACCACTTCTTCAAGCGCAACCCCCACCCTGACGCTGCCAACCCC
NM_144685.3       GCCGCCTGCAAGGCCCACCACTTCTTCAAGCGCAACCCCCACCCTGACGCTGCCAACCCC
                  ************************************************************

HsCD00037945      TGGCAGCTCAAGTCCTCGGCTGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGC
NM_144685.3       TGGCAGCTCAAGTCCTCGGCTGACTACCTGGCCGAGACGAAGGTGCGCCCATTGGAGCGC
                  ************************************************************

HsCD00037945      CGCAAGTATATGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC
NM_144685.3       CGCAAGTATATGCTCAAGTCGTTGGACCAGATTGAGACAGTGAATGGTGGCAGTGTGGCC
                  ************************************************************

HsCD00037945      AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCTCAAGAGCATG
NM_144685.3       AGTCGGCTAACCTTCCCTGACCGGGAGGCGCTGGCGGAGCACGCCGACCTCAAGAGCATG
                  ************************************************************

HsCD00037945      GTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACGAACGCATCAGCCCCAGTGCT
NM_144685.3       GTGGAGCTGATCAAGCGCATGCTGACCTGGGAGTCACACGAACGCATCAGCCCCAGTGCT
                  ************************************************************

HsCD00037945      GCCCTGCGCCACCCCTTCGTGTCCATGCAGCAGCTGCGCAGTGCCCACGAGACCACCCAC
NM_144685.3       GCCCTGCGCCACCCCTTCGTGTCCATGCAGCAGCTGCGCAGTGCCCACGAGACCACCCAC
                  ************************************************************

HsCD00037945      TACTACCAGCTCTCGCTGCGCAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCC
NM_144685.3       TACTACCAGCTCTCGCTGCGCAGCTACCGCCTCTCGCTGCAAGTGGAGGGGAAGCCCCCC
                  ************************************************************

HsCD00037945      ACGCCCGTCGTGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG
NM_144685.3       ACGCCCGTCGTGGCCGCAGAAGATGGGACCCCCTACTACTGTCTGGCTGAGGAGAAGGAG
                  ************************************************************

HsCD00037945      GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGAGAAGGCACCA
NM_144685.3       GCTGCGGGTATGGGCAGTGTGGCCGGCAGCAGCCCCTTCTTCCGAGAGGAGAAGGCACCA
                  ************************************************************

HsCD00037945      GGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTCTGCAGGAGGCTGGGCATGGG
NM_144685.3       GGTATGCAAAGAGCCATCGACCAGCTGGATGACCTGAGTCTGCAGGAGGCTGGGCATGGG
                  ************************************************************

HsCD00037945      CTGTGGGGTGAGACCTGCACCAATGCGGTCTCCGACATGATGGTCCCCCTCAAGGCAGCC
NM_144685.3       CTGTGGGGTGAGACCTGCACCAATGCGGTCTCCGACATGATGGTCCCCCTCAAGGCAGCC
                  ************************************************************

HsCD00037945      ATCACTGGCCACCATGTGCCCGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGC
NM_144685.3       ATCACTGGCCACCATGTGCCCGACTCGGGCCCTGAGCCCATCCTGGCCTTCTACAGCAGC
                  ************************************************************

HsCD00037945      CGCCTGGCAGGCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC
NM_144685.3       CGCCTGGCAGGCCGCCACAAGGCCCGCAAGCCACCTGCGGGTTCCAAGTCCGACTCCAAC
                  ************************************************************

HsCD00037945      TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCCCTGCCGGGGC
NM_144685.3       TTCAGCAACCTCATTCGGCTGAGCCAGGTCTCGCCTGAGGATGACAGGCCCTGCCGGGGC
                  ************************************************************

HsCD00037945      AGCAGCTGGGAGGAAGGAGAGTATCTCGGGGCCTCTGCTGAGCCACTGGCCATCCTGCAG
NM_144685.3       AGCAGCTGGGAGGAAGGAGAGCATCTCGGGGCCTCTGCTGAGCCACTGGCCATCCTGCAG
                  ********************* **************************************

HsCD00037945      CGAGATGAGGATGGGCCCAACATTGACAACATGACCATGGAAGCTGAGAGGCCAGACCCT
NM_144685.3       CGAGATGAGGATGGGCCCAACATTGACAACATGACCATGGAAGCTGAGAGGCCAGACCCT
                  ************************************************************

HsCD00037945      GAGCTCTTCGACCCCAGCAGCTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTG
NM_144685.3       GAGCTCTTCGACCCCAGCAGCTGTCCTGGAGAATGGCTGAGTGAGCCAGACTGCACCCTG
                  ************************************************************

HsCD00037945      GAGAGCGTCAGGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT
NM_144685.3       GAGAGCGTCAGGGGCCCACGGGCTCAGGGGCTCCCACCCCGCCGCTCCCACCAGCATGGT
                  ************************************************************

HsCD00037945      CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCAATTG
NM_144685.3       CCACCCCGGGGGGCCACCAGCTTCCTCCAGCATGTCACCGGGCACCACTGA
                  ***********************************************.* .

Clone

Cloning Information : 164619
HIP Master Clone ID : 124255
Original Clone ID : FLH164619.01L

Organism


TAX_ID : 9606
Species Specific ID: 147746

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000160396

Reagents

Labome : HIPK4-antibody