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SNARK (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  NUAK2
Gene Name:  NUAK family, SNF1-like kinase, 2
Sequence             
Original Clone ID: FLH164624.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00037950

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1887nts         Open reading frame : 1 to 1887
Reference Sequence Alignment

ATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCC
CGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTG
AAGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGC
AAAGGCACCTACGGGAAGGTGAAGAAGGCGCGGGAGAGCTCGGGGCGCCTGGTGGCCATC
AAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAG
ATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGAAGTGTTTGAG
AACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTAC
ATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTC
TCTGCCGTGCACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAAC
ATCCTCTTGGATGCCAATGGGAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTAC
CATCAAGGCAAGTTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTCGCCAGAGATT
GTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCTGGTCCCTGGGTGTTCTCCTC
TACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGATCCTAGTGAAA
CAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATC
CGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCAC
TGGTGGGTCAACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGT
GGGCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCC
CGCCCCCTCCTGGAGAATGGGGCCAAGGTGTGCAGCTTCTTCAAGCAGCATGCACCTGGT
GGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGCTCAAGAAGTCCCGCAAGGAG
AATGACATGGCCCAGTCTCTCCACAGTGACACGGCTGATGACACTGCCCATCGCCCTGGC
AAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAA
GGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCC
CCCCTGCTCCCCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTAC
TCCTCTCCCGAGCCCAGTGAATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGT
GGGGATCCCAAGGAGCAGAAGCCTCCGCAAGCTTCAGGGCTGCTCCTCCATCGCAAAGGC
ATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGGAGCTCGCGGCCCCCACCACC
TTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAGCCGACCCTCA
GGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCT
GAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTT
GAGGAGCCCCCCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTG
GGGGACAGCTGCTTTTCCCTGACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCA
CTGAGGGTCTGCTCAAAGCTCACCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 2014
Start on reference sequence 128
Mutations:
None
Discrepancy:
g1323c
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NUAK2
Symbol Nomenclature : NUAK2
SYNONYM : SNARK
Designation : NUAK family SNF1-like kinase 2|SNF1/AMP activated protein kinase|SNF1/AMP kinase-related kinase|omphalocele kinase 2
Full Nomenclature : NUAK family, SNF1-like kinase, 2
GENEID : 81788
HGNC : 29558
MIM : 608131
Vega : OTTHUMG00000037196
Target GenBank: NM_030952

 

Reference Sequence Alignment

  

HsCD00037950      ATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCC
NM_030952.1       ATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCC
                  ************************************************************

HsCD00037950      CGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTG
NM_030952.1       CGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTG
                  ************************************************************

HsCD00037950      AAGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGC
NM_030952.1       AAGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGC
                  ************************************************************

HsCD00037950      AAAGGCACCTACGGGAAGGTGAAGAAGGCGCGGGAGAGCTCGGGGCGCCTGGTGGCCATC
NM_030952.1       AAAGGCACCTACGGGAAGGTGAAGAAGGCGCGGGAGAGCTCGGGGCGCCTGGTGGCCATC
                  ************************************************************

HsCD00037950      AAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAG
NM_030952.1       AAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAG
                  ************************************************************

HsCD00037950      ATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGAAGTGTTTGAG
NM_030952.1       ATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGAAGTGTTTGAG
                  ************************************************************

HsCD00037950      AACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTAC
NM_030952.1       AACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTAC
                  ************************************************************

HsCD00037950      ATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTC
NM_030952.1       ATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTC
                  ************************************************************

HsCD00037950      TCTGCCGTGCACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAAC
NM_030952.1       TCTGCCGTGCACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAAC
                  ************************************************************

HsCD00037950      ATCCTCTTGGATGCCAATGGGAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTAC
NM_030952.1       ATCCTCTTGGATGCCAATGGGAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTAC
                  ************************************************************

HsCD00037950      CATCAAGGCAAGTTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTCGCCAGAGATT
NM_030952.1       CATCAAGGCAAGTTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTCGCCAGAGATT
                  ************************************************************

HsCD00037950      GTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCTGGTCCCTGGGTGTTCTCCTC
NM_030952.1       GTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCTGGTCCCTGGGTGTTCTCCTC
                  ************************************************************

HsCD00037950      TACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGATCCTAGTGAAA
NM_030952.1       TACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGATCCTAGTGAAA
                  ************************************************************

HsCD00037950      CAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATC
NM_030952.1       CAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATC
                  ************************************************************

HsCD00037950      CGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCAC
NM_030952.1       CGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCAC
                  ************************************************************

HsCD00037950      TGGTGGGTCAACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGT
NM_030952.1       TGGTGGGTCAACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGT
                  ************************************************************

HsCD00037950      GGGCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCC
NM_030952.1       GGGCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCC
                  ************************************************************

HsCD00037950      CGCCCCCTCCTGGAGAATGGGGCCAAGGTGTGCAGCTTCTTCAAGCAGCATGCACCTGGT
NM_030952.1       CGCCCCCTCCTGGAGAATGGGGCCAAGGTGTGCAGCTTCTTCAAGCAGCATGCACCTGGT
                  ************************************************************

HsCD00037950      GGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGCTCAAGAAGTCCCGCAAGGAG
NM_030952.1       GGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGCTCAAGAAGTCCCGCAAGGAG
                  ************************************************************

HsCD00037950      AATGACATGGCCCAGTCTCTCCACAGTGACACGGCTGATGACACTGCCCATCGCCCTGGC
NM_030952.1       AATGACATGGCCCAGTCTCTCCACAGTGACACGGCTGATGACACTGCCCATCGCCCTGGC
                  ************************************************************

HsCD00037950      AAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAA
NM_030952.1       AAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAA
                  ************************************************************

HsCD00037950      GGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCC
NM_030952.1       GGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCC
                  ************************************************************

HsCD00037950      CCCCTGCTCCCCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTAC
NM_030952.1       CCGCTGCTCCCCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTAC
                  ** *********************************************************

HsCD00037950      TCCTCTCCCGAGCCCAGTGAATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGT
NM_030952.1       TCCTCTCCCGAGCCCAGTGAATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGT
                  ************************************************************

HsCD00037950      GGGGATCCCAAGGAGCAGAAGCCTCCGCAAGCTTCAGGGCTGCTCCTCCATCGCAAAGGC
NM_030952.1       GGGGATCCCAAGGAGCAGAAGCCTCCGCAAGCTTCAGGGCTGCTCCTCCATCGCAAAGGC
                  ************************************************************

HsCD00037950      ATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGGAGCTCGCGGCCCCCACCACC
NM_030952.1       ATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGGAGCTCGCGGCCCCCACCACC
                  ************************************************************

HsCD00037950      TTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAGCCGACCCTCA
NM_030952.1       TTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAGCCGACCCTCA
                  ************************************************************

HsCD00037950      GGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCT
NM_030952.1       GGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCT
                  ************************************************************

HsCD00037950      GAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTT
NM_030952.1       GAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTT
                  ************************************************************

HsCD00037950      GAGGAGCCCCCCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTG
NM_030952.1       GAGGAGCCCCCCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTG
                  ************************************************************

HsCD00037950      GGGGACAGCTGCTTTTCCCTGACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCA
NM_030952.1       GGGGACAGCTGCTTTTCCCTGACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCA
                  ************************************************************

HsCD00037950      CTGAGGGTCTGCTCAAAGCTCACCTTG
NM_030952.1       CTGAGGGTCTGCTCAAAGCTCACCTGA
                  ************************* .

Clone

Cloning Information : 164624
HIP Master Clone ID : 124262
Original Clone ID : FLH164624.01L

Organism


TAX_ID : 9606
Species Specific ID: 81788

Genome

Chromosome : 1
Map Location : 1q32.1
Ensembl : ENSG00000163545

Reagents

Labome : SNARK-antibody