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STK29 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  BRSK2
Gene Name:  BR serine/threonine kinase 2
Sequence             
Original Clone ID: FLH164631.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00037954

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 513nts         Open reading frame : 1 to 513
Reference Sequence Alignment

ATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCCTGGTTTGGGAAC
TTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGACAAACCTCTGAGC
TCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTCAGCCACAGCGTC
ATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCAGCCGTGTTCCAG
AAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAGGCGCAGAAGGAG
AACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGTCGCTTCAAGAGG
GTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCTGCGGCCCAGCAC
TTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGGCTTAAGGGCCAG
AAGGTGGCCACCAGCTACGAGAGTAGCCTCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 667
Start on reference sequence 155
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BRSK2
Symbol Nomenclature : BRSK2
SYNONYM : C11orf7; PEN11B; SAD1; SADA; STK29
Designation : BR serine/threonine-protein kinase 2|brain-selective kinase 2|brain-specific serine/threonine-protein kinase 2|protein kinase SAD1B|serine/threonine-protein kinase 29|serine/threonine-protein kinase BRSK2|serine/threonine-protein kinase SAD-A
Full Nomenclature : BR serine/threonine kinase 2
GENEID : 9024
Locus Tag : HUSSY-12
HGNC : 11405
MIM : 609236
Vega : OTTHUMG00000167089
Target GenBank: BC024291

 

Reference Sequence Alignment

  

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      ATGACATCGACGGGGAAGGACGGCGGCGCGCAGCACGCGCAGTATGTTGGGCCCTACCGG
NM_001282218.1      ------------------------------------------------------------
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      CTGGAGAAGACGCTGGGCAAGGGGCAGACAGGTCTGGTGAAGCTGGGGGTTCACTGCGTC
NM_001282218.1      ------------------------------------------------------------
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      ACCTGCCAGAAGGTGGCCATCAAGATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTG
NM_001282218.1      ------------------------------------------------------------
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      ATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAG
NM_001282218.1      ATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      CTGCACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGT
NM_001282218.1      CTGCACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGT
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      GGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAG
NM_001282218.1      GGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      TTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGG
NM_001282218.1      TTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      GATCTGAAACCTGAAAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGACTTT
NM_001282218.1      GATCTGAAACCTGAAAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGACTTT
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      GGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCAC
NM_001282218.1      GGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCAC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      TACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGG
NM_001282218.1      TACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      AGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAAC
NM_001282218.1      AGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAAC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      TTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCG
NM_001282218.1      TTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      CCCGACTGCCAGAGTCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACG
NM_001282218.1      CCCGACTGCCAGAGTCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      CTAGAGCACATTCAGAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAG
NM_001282218.1      CTAGAGCACATTCAGAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      CAGCCCATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCC
NM_001282218.1      CAGCCCATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      GACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAG
NM_001282218.1      GACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      GACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGG
NM_001282218.1      GACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGG
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      AAAGAAAGGTACCCGAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGACCCT
NM_001282218.1      AAAGAAAGGTACCCGAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGACCCT
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      CCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGC
NM_001282218.1      CCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      AAATCCATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCC
NM_001282218.1      AAATCCATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      ATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTCCTCAGGCCTT
NM_001282218.1      ATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTCCTCAGGCCTT
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      TCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCC
NM_001282218.1      TCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      CTCCCCACCCCCAAGGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGCTGGCACGCCC
NM_001282218.1      CTCCCCACCCCCAAGGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGCTGGCACGCCC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      AACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAAC
NM_001282218.1      AACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAAC
                                                                                

HsCD00037954        ------------------------------------------------------------
NM_001256629.1      TCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCG
NM_001282218.1      TCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCG
                                                                                

HsCD00037954        ------------ATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCC
NM_001256629.1      ACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCC
NM_001282218.1      ACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCC
                                ************************************************

HsCD00037954        TGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGAC
NM_001256629.1      TGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGAC
NM_001282218.1      TGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGAC
                    ************************************************************

HsCD00037954        AAACCTCTGAGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTC
NM_001256629.1      AAACCTCTGAGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTC
NM_001282218.1      AAACCTCTGAGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTC
                    ************************************************************

HsCD00037954        AGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCA
NM_001256629.1      AGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCA
NM_001282218.1      AGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCA
                    ************************************************************

HsCD00037954        GCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAG
NM_001256629.1      GCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAG
NM_001282218.1      GCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAG
                    ************************************************************

HsCD00037954        GCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGT
NM_001256629.1      GCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGT
NM_001282218.1      GCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGT
                    ************************************************************

HsCD00037954        CGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCT
NM_001256629.1      CGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCT
NM_001282218.1      CGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCT
                    ************************************************************

HsCD00037954        GCGGCCCAGCACTTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGG
NM_001256629.1      GCGGCCCAGCACTTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGG
NM_001282218.1      GCGGCCCAGCACTTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGG
                    ************************************************************

HsCD00037954        CTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTCTTG
NM_001256629.1      CTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTCTGA
NM_001282218.1      CTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTCTGA
                    ******************************************* .

Pathways

NCI : LKB1 signaling events

Protein

UniProt : Q8IWQ3

Clone

Cloning Information : 164631
HIP Master Clone ID : 124269
Original Clone ID : FLH164631.01L

Organism


TAX_ID : 9606
Species Specific ID: 9024

Genome

Chromosome : 11
Map Location : 11p15.5
Ensembl : ENSG00000174672

Reagents

Labome : BRSK2-antibody