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MGC22688 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  STK32A
Gene Name:  serine/threonine kinase 32A
Sequence             
Original Clone ID: FLH164632.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00037955

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 501nts         Open reading frame : 1 to 501
Reference Sequence Alignment

ATGGGAGCGAACACTTCAAGAAAACCACCAGTGTTTGATGAAAATGAAGATGTCAACTTT
GACCACTTTGAAATTTTGCGAGCCATTGGGAAAGGCAGTTTTGGGAAGGTCTGCATTGTA
CAGAAGAATGATACCAAGAAGATGTACGCAATGAAGTACATGAATAAACAAAAGTGCGTG
GAGCGCAATGAAGTGAGAAATGTCTTCAAGGAACTCCAGATCATGCAGGGTCTGGAGCAC
CCTTTCCTGGTTAATTTGTGGTATTCCTTCCAAGATGAGGAAGACATGTTCATGGTGGTG
GACCTCCTGCTGGGTGGAGACCTGCGTTATCACCTGCAACAGAACGTCCACTTCAAGGAA
GAAACAGTGAAGCTCTTCATCTGTGAGCTGGTCATGGCCCTGGACTACCTGCAGAACCAG
CGCATCATTCACAGGGATATGAAGCCTGACAATATTTTACTTGACGAACATGATACCTGG
CTCTCCTACAAGTCCCACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 691
Start on reference sequence 191
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STK32A
Symbol Nomenclature : STK32A
SYNONYM : YANK1
Designation : A930015B13Rik|serine/threonine-protein kinase 32A|yet another novel kinase 1
Full Nomenclature : serine/threonine kinase 32A
GENEID : 202374
HGNC : 28317
Vega : OTTHUMG00000163411
Target GenBank: XM_114476

 

Reference Sequence Alignment

  

HsCD00037955        ATGGGAGCGAACACTTCAAGAAAACCACCAGTGTTTGATGAAAATGAAGATGTCAACTTT
NM_145001.3         ATGGGAGCGAACACTTCAAGAAAACCACCAGTGTTTGATGAAAATGAAGATGTCAACTTT
NM_001287740.1      ATGGGAGCGAACACTTCAAGAAAACCACCAGTGTTTGATGAAAATGAAGATGTCAACTTT
NM_001112724.1      ATGGGAGCGAACACTTCAAGAAAACCACCAGTGTTTGATGAAAATGAAGATGTCAACTTT
                    ************************************************************

HsCD00037955        GACCACTTTGAAATTTTGCGAGCCATTGGGAAAGGCAGTTTTGGGAAGGTCTGCATTGTA
NM_145001.3         GACCACTTTGAAATTTTGCGAGCCATTGGGAAAGGCAGTTTTGGGAAGGTCTGCATTGTA
NM_001287740.1      GACCACTTTGAAATTTTGCGAGCCATTGGGAAAGGCAGTTTTGGGAAGGTCTGCATTGTA
NM_001112724.1      GACCACTTTGAAATTTTGCGAGCCATTGGGAAAGGCAGTTTTGGGAAGGTCTGCATTGTA
                    ************************************************************

HsCD00037955        CAGAAGAATGATACCAAGAAGATGTACGCAATGAAGTACATGAATAAACAAAAGTGCGTG
NM_145001.3         CAGAAGAATGATACCAAGAAGATGTACGCAATGAAGTACATGAATAAACAAAAGTGCGTG
NM_001287740.1      CAGAAGAATGATACCAAGAAGATGTACGCAATGAAGTACATGAATAAACAAAAGTGCGTG
NM_001112724.1      CAGAAGAATGATACCAAGAAGATGTACGCAATGAAGTACATGAATAAACAAAAGTGCGTG
                    ************************************************************

HsCD00037955        GAGCGCAATGAAGTGAGAAATGTCTTCAAGGAACTCCAGATCATGCAGGGTCTGGAGCAC
NM_145001.3         GAGCGCAATGAAGTGAGAAATGTCTTCAAGGAACTCCAGATCATGCAGGGTCTGGAGCAC
NM_001287740.1      GAGCGCAATGAAGTGAGAAATGTCTTCAAGGAACTCCAGATCATGCAGGGTCTGGAGCAC
NM_001112724.1      GAGCGCAATGAAGTGAGAAATGTCTTCAAGGAACTCCAGATCATGCAGGGTCTGGAGCAC
                    ************************************************************

HsCD00037955        CCTTTCCTGGTTAATTTGTGGTATTCCTTCCAAGATGAGGAAGACATGTTCATGGTGGTG
NM_145001.3         CCTTTCCTGGTTAATTTGTGGTATTCCTTCCAAGATGAGGAAGACATGTTCATGGTGGTG
NM_001287740.1      CCTTTCCTGGTTAATTTGTGGTATTCCTTCCAAGATGAGGAAGACATGTTCATGGTGGTG
NM_001112724.1      CCTTTCCTGGTTAATTTGTGGTATTCCTTCCAAGATGAGGAAGACATGTTCATGGTGGTG
                    ************************************************************

HsCD00037955        GACCTCCTGCTGGGTGGAGACCTGCGTTATCACCTGCAACAGAACGTCCACTTCAAGGAA
NM_145001.3         GACCTCCTGCTGGGTGGAGACCTGCGTTATCACCTGCAACAGAACGTCCACTTCAAGGAA
NM_001287740.1      GACCTCCTGCTGGGTGGAGACCTGCGTTATCACCTGCAACAGAACGTCCACTTCAAGGAA
NM_001112724.1      GACCTCCTGCTGGGTGGAGACCTGCGTTATCACCTGCAACAGAACGTCCACTTCAAGGAA
                    ************************************************************

HsCD00037955        GAAACAGTGAAGCTCTTCATCTGTGAGCTGGTCATGGCCCTGGACTACCTGCAGAACCAG
NM_145001.3         GAAACAGTGAAGCTCTTCATCTGTGAGCTGGTCATGGCCCTGGACTACCTGCAGAACCAG
NM_001287740.1      GAAACAGTGAAGCTCTTCATCTGTGAGCTGGTCATGGCCCTGGACTACCTGCAGAACCAG
NM_001112724.1      GAAACAGTGAAGCTCTTCATCTGTGAGCTGGTCATGGCCCTGGACTACCTGCAGAACCAG
                    ************************************************************

HsCD00037955        CGCATCATTCACAGGGATATGAAGCCTGACAATATTTTACTTGACGAACATGATACCTGG
NM_145001.3         CGCATCATTCACAGGGATATGAAGCCTGACAATATTTTACTTGACGAACATGATACCTGG
NM_001287740.1      CGCATCATTCACAGGGATATGAAGCCTGACAATATTTTACTTGACGAACATGGGCACGTG
NM_001112724.1      CGCATCATTCACAGGGATATGAAGCCTGACAATATTTTACTTGACGAACATGGGCACGTG
                    ****************************************************. ..*  *

HsCD00037955        CTCTCCTACAAGTCCCACTTG---------------------------------------
NM_145001.3         CTCTCCTACAAGTCCCACTGA---------------------------------------
NM_001287740.1      CACATCACAGATTTCAACATTGCTGCGATGCTGCCCAGGGAGACACAGATTACCACCATG
NM_001112724.1      CACATCACAGATTTCAACATTGCTGCGATGCTGCCCAGGGAGACACAGATTACCACCATG
                    *:*: *:...* * *.**:                                         

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      GCTGGCACCAAGCCTTACATGGCACCTGAGATGTTCAGCTCCAGAAAAGGAGCAGGCTAT
NM_001112724.1      GCTGGCACCAAGCCTTACATGGCACCTGAGATGTTCAGCTCCAGAAAAGGAGCAGGCTAT
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      TCCTTTGCTGTTGACTGGTGGTCCCTGGGAGTGACGGCATATGAACTGCTGAGAGGCCGG
NM_001112724.1      TCCTTTGCTGTTGACTGGTGGTCCCTGGGAGTGACGGCATATGAACTGCTGAGAGGCCGG
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      AGACCGTATCATATTCGCTCCAGTACTTCCAGCAAGGAAATTGTACACACGTTTGAGACG
NM_001112724.1      AGACCGTATCATATTCGCTCCAGTACTTCCAGCAAGGAAATTGTACACACGTTTGAGACG
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      ACTGTTGTAACTTACCCTTCTGCCTGGTCACAGGAAATGGTGTCACTTCTTAAAAAGCTA
NM_001112724.1      ACTGTTGTAACTTACCCTTCTGCCTGGTCACAGGAAATGGTGTCACTTCTTAAAAAGCTA
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      CTCGAACCTAATCCAGACCAACGATTTTCTCAGTTATCTGATGTCCAGAACTTCCCGTAT
NM_001112724.1      CTCGAACCTAATCCAGACCAACGATTTTCTCAGTTATCTGATGTCCAGAACTTCCCGTAT
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      ATGAATGATATAAACTGGGATGCAGTTTTTCAGAAGAGGCTCATTCCAGGTTTCATTCCT
NM_001112724.1      ATGAATGATATAAACTGGGATGCAGTTTTTCAGAAGAGGCTCATTCCAGGTTTCATTCCT
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      AATAAAGGCAGGCTGAATTGTGATCCTACCTTTGAACTTGAGGAAATGATTTTGGAGTCC
NM_001112724.1      AATAAAGGCAGGCTGAATTGTGATCCTACCTTTGAACTTGAGGAAATGATTTTGGAGTCC
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      AAACCTCTACATAAGAAAAAAAAGCGTCTGGCAAAGAAGGAGAAGGATATGAGGAAATGC
NM_001112724.1      AAACCTCTACATAAGAAAAAAAAGCGTCTGGCAAAGAAGGAGAAGGATATGAGGAAATGC
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      GATTCTTCTCAGACATGTCTTCTTCAAGAGCACCTTGACTCTGTCCAGAAGGAGTTCATA
NM_001112724.1      GATTCTTCTCAGACATGTCTTCTTCAAGAGCACCTTGACTCTGTCCAGAAGGAGTTCATA
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      ATTTTCAACAGAGAAAAAGTAAACAGGGACTTTAACAAAAGACAACCAAATCTAGCCTTG
NM_001112724.1      ATTTTCAACAGAGAAAAAGTAAACAGGGACTTTAACAAAAGACAACCAAATCTAGCCTTG
                                                                                

HsCD00037955        ------------------------------------------------------------
NM_145001.3         ------------------------------------------------------------
NM_001287740.1      GAACAAACCAAAGACCCACAAGTGACAAATGGACAAATGGACACAGGACTCAGTGAGACT
NM_001112724.1      GAACAAACCAAAGACCCACAAGGTGAGGATGGTCAGAATAACAACTTGTAA---------
                                                                                

HsCD00037955        ------------------------
NM_145001.3         ------------------------
NM_001287740.1      TTTCAGACCTCGAAAGTTTCATAA
NM_001112724.1      ------------------------
                                            

Clone

Cloning Information : 164632
HIP Master Clone ID : 124270
Original Clone ID : FLH164632.01L

Organism


TAX_ID : 9606
Species Specific ID: 202374

Genome

Chromosome : 5
Map Location : 5q32
Ensembl : ENSG00000169302

Reagents

Labome : STK32A-antibody