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NJMU-R1 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  C17orf75
Gene Name:  chromosome 17 open reading frame 75
Sequence             
Original Clone ID: FLH164633.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00037956

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1101nts         Open reading frame : 1 to 1101
Reference Sequence Alignment

ATGCTCCCCTCTTTGCAGGAGTCGATGGATGGAGATGAAAAGGAACTAGAGAGCAGCGAA
GAGGGAGGCTCAGCCGAGGAGCGGAGACTCGAGCCGCCGTCCAGCAGCCACTACTGTCTT
TACAGCTATCGCGGAAGCAGATTGGCACAGCAACGAGGGGACAGTGAGGACGGAAGCCCA
AGTGGCACAAATGCAGAAACTCCCTCTGGTGATGATTTCAGCCTCTCCTTGGCAGATACT
AATCTACCATCCGAAGTGGAGCCAGAGCTGCGCAGTTTCATTGCTAAGCGTCTTTCAAGA
GGTGCAGTCTTTGAAGGGCTGGGTAATGTTGCATCTGTGGAGCTAAAAATTCCAGGTTAC
CGAGTTGGTTGTTATTACTGCCTTTTCCAAAATGAAAAACTGCTTCCTGAAACAGTAACG
ATAGACTCTGAACGTAACCCTTCAGAATATGTGGTCTGTTTTTTAGGAGGGTCTGAAAAA
GGACTTGAGCTTTTCAGGCTTGAATTGGACAAGTACATTCAAGGGCTGAAAAATAACATG
AATTGTGAGGCAAGGGGCCTGGAGAGTCACATAAAATCCTATCTGAGCAGCTGGTTTGAG
GATGTTGTATGCCCAATCCAAAGGGTTGTTCTTCTCTTTCAGGAAAAGCTTACCTTTCTG
CTACATGCTGCTTTGAGTTACACTCCTGTTGAAGTTAAAGAATCAGATGAAAAAACAAAG
AGAGACATTAACAGGTTTCTGAGTGTGGCCAGTCTTCAAGGACTTATTCATGAAGGCACC
ATGACTTCTTTGTGCATGGCCATGACAGAGGAGCAGCATAAGTCTGTGGTCATCGATTGC
AGCAGCTCCCAGCCTCAGTTCTGCAATGCAGGAAGTAACCGGTTTTGTGAGGATTGGATG
CAAGCTTTTTTAAATGGTGCCAAAGGAGGTAACCCTTTTTTTTTCCGACAAGTACTGGAG
AACTTTAAACTAAAGGCCATACAAGACACAAACAATTTGAAGAGATTTATCCGACAGGCA
GAAATGAATCATTATGCTTTGTTTAAATGTTACATGTTCCTAAAGAACTGTGGTAGTGGA
GATACTTTTGAAGATTGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1127
Start on reference sequence 27
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : C17orf75
Symbol Nomenclature : C17orf75
SYNONYM : NJMU-R1
Designation : protein Njmu-R1|spermatogenesis-related protein
Full Nomenclature : chromosome 17 open reading frame 75
GENEID : 64149
HGNC : 30173
Target GenBank: NM_022344

 

Reference Sequence Alignment

  

HsCD00037956        ATGCTCCCCTCTTTGCAGGAGTCGATGGATGGAGATGAAAAGGAACTAGAGAGCAGCGAA
XM_005258022.1      ------------------------ATGGATGGAGATGAAAAGGAACTAGAGAGCAGCGAA
NM_022344.3         ATGCTCCCCTCTTTGCAGGAGTCGATGGATGGAGATGAAAAGGAACTAGAGAGCAGCGAA
                                            ************************************

HsCD00037956        GAGGGAGGCTCAGCCGAGGAGCGGAGACTCGAGCCGCCGTCCAGCAGCCACTACTGTCTT
XM_005258022.1      GAGGGAGGCTCAGCCGAGGAGCGGAGACTCGAGCCGCCGTCCAGCAGCCACTACTGTCTT
NM_022344.3         GAGGGAGGCTCAGCCGAGGAGCGGAGACTCGAGCCGCCGTCCAGCAGCCACTACTGTCTT
                    ************************************************************

HsCD00037956        TACAGCTATCGCGGAAGCAGATTGGCACAGCAACGAGGGGACAGTGAGGACGGAAGCCCA
XM_005258022.1      TACAGCTATCGCGGAAGCAGATTGGCACAGCAACGAGGGGACAGTGAGGACGGAAGCCCA
NM_022344.3         TACAGCTATCGCGGAAGCAGATTGGCACAGCAACGAGGGGACAGTGAGGACGGAAGCCCA
                    ************************************************************

HsCD00037956        AGTGGCACAAATGCAGAAACTCCCTCTGGTGATGATTTCAGCCTCTCCTTGGCAGATACT
XM_005258022.1      AGTGGCACAAATGCAGAAACTCCCTCTGGTGATGATTTCAGCCTCTCCTTGGCAGATACT
NM_022344.3         AGTGGCACAAATGCAGAAACTCCCTCTGGTGATGATTTCAGCCTCTCCTTGGCAGATACT
                    ************************************************************

HsCD00037956        AATCTACCATCCGAAGTGGAGCCAGAGCTGCGCAGTTTCATTGCTAAGCGTCTTTCAAGA
XM_005258022.1      AATCTACCATCCGAAGTGGAGCCAGAGCTGCGCAGTTTCATTGCTAAGCGTCTTTCAAGA
NM_022344.3         AATCTACCATCCGAAGTGGAGCCAGAGCTGCGCAGTTTCATTGCTAAGCGTCTTTCAAGA
                    ************************************************************

HsCD00037956        GGTGCAGTCTTTGAAGGGCTGGGTAATGTTGCATCTGTGGAGCTAAAAATTCCAGGTTAC
XM_005258022.1      GGTGCAGTCTTTGAAGGGCTGGGTAATGTTGCATCTGTGGAGCTAAAAATTCCAGGTTAC
NM_022344.3         GGTGCAGTCTTTGAAGGGCTGGGTAATGTTGCATCTGTGGAGCTAAAAATTCCAGGTTAC
                    ************************************************************

HsCD00037956        CGAGTTGGTTGTTATTACTGCCTTTTCCAAAATGAAAAACTGCTTCCTGAAACAGTAACG
XM_005258022.1      CGAGTTGGTTGTTATTACTGCCTTTTCCAAAATGAAAAACTGCTTCCTGAAACAGTAACG
NM_022344.3         CGAGTTGGTTGTTATTACTGCCTTTTCCAAAATGAAAAACTGCTTCCTGAAACAGTAACG
                    ************************************************************

HsCD00037956        ATAGACTCTGAACGTAACCCTTCAGAATATGTGGTCTGTTTTTTAGGAGGGTCTGAAAAA
XM_005258022.1      ATAGACTCTGAACGTAACCCTTCAGAATATGTGGTCTGTTTTTTAGGAGGGTCTGAAAAA
NM_022344.3         ATAGACTCTGAACGTAACCCTTCAGAATATGTGGTCTGTTTTTTAGGAGGGTCTGAAAAA
                    ************************************************************

HsCD00037956        GGACTTGAGCTTTTCAGGCTTGAATTGGACAAGTACATTCAAGGGCTGAAAAATAACATG
XM_005258022.1      GGACTTGAGCTTTTCAGGCTTGAATTGGACAAGTACATTCAAGGGCTGAAAAATAACATG
NM_022344.3         GGACTTGAGCTTTTCAGGCTTGAATTGGACAAGTACATTCAAGGGCTGAAAAATAACATG
                    ************************************************************

HsCD00037956        AATTGTGAGGCAAGGGGCCTGGAGAGTCACATAAAATCCTATCTGAGCAGCTGGTTTGAG
XM_005258022.1      AATTGTGAGGCAAGGGGCCTGGAGAGTCACATAAAATCCTATCTGAGCAGCTGGTTTGAG
NM_022344.3         AATTGTGAGGCAAGGGGCCTGGAGAGTCACATAAAATCCTATCTGAGCAGCTGGTTTGAG
                    ************************************************************

HsCD00037956        GATGTTGTATGCCCAATCCAAAGGGTTGTTCTTCTCTTTCAGGAAAAGCTTACCTTTCTG
XM_005258022.1      GATGTTGTATGCCCAATCCAAAGGGTTGTTCTTCTCTTTCAGGAAAAGCTTACCTTTCTG
NM_022344.3         GATGTTGTATGCCCAATCCAAAGGGTTGTTCTTCTCTTTCAGGAAAAGCTTACCTTTCTG
                    ************************************************************

HsCD00037956        CTACATGCTGCTTTGAGTTACACTCCTGTTGAAGTTAAAGAATCAGATGAAAAAACAAAG
XM_005258022.1      CTACATGCTGCTTTGAGTTACACTCCTGTTGAAGTTAAAGAATCAGATGAAAAAACAAAG
NM_022344.3         CTACATGCTGCTTTGAGTTACACTCCTGTTGAAGTTAAAGAATCAGATGAAAAAACAAAG
                    ************************************************************

HsCD00037956        AGAGACATTAACAGGTTTCTGAGTGTGGCCAGTCTTCAAGGACTTATTCATGAAGGCACC
XM_005258022.1      AGAGACATTAACAGGTTTCTGAGTGTGGCCAGTCTTCAAGGACTTATTCATGAAGGCACC
NM_022344.3         AGAGACATTAACAGGTTTCTGAGTGTGGCCAGTCTTCAAGGACTTATTCATGAAGGCACC
                    ************************************************************

HsCD00037956        ATGACTTCTTTGTGCATGGCCATGACAGAGGAGCAGCATAAGTCTGTGGTCATCGATTGC
XM_005258022.1      ATGACTTCTTTGTGCATGGCCATGACAGAGGAGCAGCATAAGTCTGTGGTCATCGATTGC
NM_022344.3         ATGACTTCTTTGTGCATGGCCATGACAGAGGAGCAGCATAAGTCTGTGGTCATCGATTGC
                    ************************************************************

HsCD00037956        AGCAGCTCCCAGCCTCAGTTCTGCAATGCAGGAAGTAACCGGTTTTGTGAGGATTGGATG
XM_005258022.1      AGCAGCTCCCAGCCTCAGTTCTGCAATGCAGGAAGTAACCGGTTTTGTGAGGATTGGATG
NM_022344.3         AGCAGCTCCCAGCCTCAGTTCTGCAATGCAGGAAGTAACCGGTTTTGTGAGGATTGGATG
                    ************************************************************

HsCD00037956        CAAGCTTTTTTAAATGGTGCCAAAGGAGGTAACCCTTTTTTTTTCCGACAAGTACTGGAG
XM_005258022.1      CAAGCTTTTTTAAATGGTGCCAAAGGAGGTAACCCTTTTCTTTTCCGACAAGTACTGGAG
NM_022344.3         CAAGCTTTTTTAAATGGTGCCAAAGGAGGTAACCCTTTTCTTTTCCGACAAGTACTGGAG
                    *************************************** ********************

HsCD00037956        AACTTTAAACTAAAGGCCATACAAGACACAAACAATTTGAAGAGATTTATCCGACAGGCA
XM_005258022.1      AACTTTAAACTAAAGGCCATACAAGACACAAACAATTTGAAGAGATTTATCCGACAGGCA
NM_022344.3         AACTTTAAACTAAAGGCCATACAAGACACAAACAATTTGAAGAGATTTATCCGACAGGCA
                    ************************************************************

HsCD00037956        GAAATGAATCATTATGCTTTGTTTAAATGTTACATGTTCCTAAAGAACTGTGGTAGTGGA
XM_005258022.1      GAAATGAATCATTATGCTTTGTTTAAATGTTACATGTTCCTAAAGAACTGTGGTAGTGGA
NM_022344.3         GAAATGAATCATTATGCTTTGTTTAAATGTTACATGTTCCTAAAGAACTGTGGTAGTGGA
                    ************************************************************

HsCD00037956        GATACTTTTGAAGATTGTTTG---------------------------------------
XM_005258022.1      GATATACTTTTGAAGATTGTTAAAGTGGAACATGAAGAAATGCCTGAAGCCAAAAATGTG
NM_022344.3         GATATACTTTTGAAGATTGTTAAAGTGGAACATGAAGAAATGCCTGAAGCCAAAAATGTG
                    **** : ** :..* : * *                                        

HsCD00037956        ---------------------------------------------------
XM_005258022.1      ATAGCTGTCCTTGAAGAATTCATGAAAGAAGCTCTTGACCAAAGTTTTTGA
NM_022344.3         ATAGCTGTCCTTGAAGAATTCATGAAAGAAGCTCTTGACCAAAGTTTTTGA
                                                                       

Protein

UniProt : Q9HAS0
HPRD : 11392

Clone

Cloning Information : 164633
HIP Master Clone ID : 124271
Original Clone ID : FLH164633.01L

Organism


TAX_ID : 9606
Species Specific ID: 64149

Genome

Chromosome : 17
Map Location : 17q11.2

Reagents

Labome : C17orf75-antibody