DNASU Plasmid Repository • 480.965.5697 | Email

NME6 (Homo sapiens) in pJP1520 (retroviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  NME6
Gene Name:  NME/NM23 nucleoside diphosphate kinase 6
Sequence             
Original Clone ID: FLH138521.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00037958

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 585nts         Open reading frame : 1 to 585
Reference Sequence Alignment

ATGACCCAGAATCTGGGGAGTGAGATGGCCTCAATCTTGCGAAGCCCTCAGGCTCTCCAG
CTCACTCTAGCCCTGATCAAGCCTGACGCAGTCGCCCATCCACTGATTCTGGAGGCTGTT
CATCAGCAGATTCTAAGCAACAAGTTCCTGATTGTACGAATGAGAGAACTACTGTGGAGA
AAGGAAGATTGCCAGAGGTTTTACCGAGAGCATGAAGGGCGTTTTTTCTATCAGAGGCTG
GTGGAGTTCATGGCCAGCGGGCCAATCCGAGCCTACATCCTTGCCCACAAGGATGCCATC
CAGCTCTGGAGGACGCTCATGGGACCCACCAGAGTGTTCCGAGCACGCCATGTGGCCCCA
GATTCTATCCGTGGGAGTTTCGGCCTCACTGACACCCGCAACACCACCCATGGTTCGGAC
TCTGTGGTTTCAGCCAGCAGAGAGATTGCAGCCTTCTTCCCTGACTTCAGTGAACAGCGC
TGGTATGAGGAGGAAGAGCCCCAGTTGCGCTGTGGCCCTGTGTGCTATAGCCCAGAGGGA
GGTGTCCACTATGTAGCTGGAACAGGAGGCCTAGGACCAGCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 640
Start on reference sequence 56
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NME6
Symbol Nomenclature : NME6
SYNONYM : IPIA-ALPHA; NDK 6; NM23-H6
Designation : NDP kinase 6|inhibitor of p53-induced apoptosis-alpha|non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase)|nucleoside diphosphate kinase 6
Full Nomenclature : NME/NM23 nucleoside diphosphate kinase 6
GENEID : 10201
HGNC : 20567
MIM : 608294
Vega : OTTHUMG00000133531
Target GenBank: BC001808

 

Reference Sequence Alignment

  

HsCD00037958        ------------------------------------------------------------
NM_005793.3         ------------------------------------------------------------
XM_005264792.1      ------------------------------------------------------------
XM_005264790.1      ATGTTCCAGGAACTACCCTGGGCAAAGAAAATAACTGGGCAAGGTTTCCATCGGTGTCTC
XM_005264789.1      ATGTTCCAGGAACTACCCTGGGCAAAGAAAATAACTGGGCAAGGTTTCCATCGGTGTCTC
                                                                                

HsCD00037958        ------------------------------------------------------------
NM_005793.3         ------------------------------------------------------------
XM_005264792.1      ------------------------------------------------------------
XM_005264790.1      TGCTGGCGGGCATCGCCCCCGCCGGGCCGCCACCCTGAACGAGCCCGTCCGGTCCTCTGG
XM_005264789.1      TGCTGGCGGGCATCGCCCCCGCCGGGCCGCCACCCTGAACGAGCCCGTCCGGTCCTCTGG
                                                                                

HsCD00037958        ------------------------------------------------------------
NM_005793.3         ------------------------------------------------------------
XM_005264792.1      ------------------------------------------------------------
XM_005264790.1      GTTCCGCCACCCTGCGTAGCGCTGCGCCAGCACCCGGCCCAGCACTCCCTCCCGCCGAGG
XM_005264789.1      GTTCCGCCACCCTGCGTAGCGCTGCGCCAGCACCCGGCCCAGCACTCCCTCCCGCCGAGG
                                                                                

HsCD00037958        ------------------------------------------------------------
NM_005793.3         ------------------------------------------------------------
XM_005264792.1      ------------------------------------------------------------
XM_005264790.1      GCGCCCAGGCCGGCTTCCGAGGGCGCCCAGGCCGGCTTCCGAGGGGCGGTTCCCACAAGC
XM_005264789.1      GCGCCCAGGCCGGCTTCCGAGGGCGCCCAGGCCGGCTTCCGAGGGGCGGTTCCCACAAGC
                                                                                

HsCD00037958        ------------------------------------------------------------
NM_005793.3         ------------------------------------------------------------
XM_005264792.1      ------------------------------------------------------------
XM_005264790.1      GAGTTCCGGCGCCCAGAACGGCTTCATCCTCTCGTCCCGTCCGCGGCAAATGCATGCGCC
XM_005264789.1      GAGTTCCGGCGCCCAGAACGGCTTCATCCTCTCGTCCCGTCCGCGGCAAATGCATGCGCC
                                                                                

HsCD00037958        ------------------------------------------------------------
NM_005793.3         ------------------------------------------------------------
XM_005264792.1      ------------------------------------------------------------
XM_005264790.1      TGGAAAACCCGCCACCCTCCAGGGCAACTCACACCTCACCTCACGCCGCGGCGCAACCGG
XM_005264789.1      TGGAAAACCCGCCACCCTCCAGGGCAACTCACACCTCACCTCACGCCGCGGCGCAACCGG
                                                                                

HsCD00037958        ------------------------------------------------------------
NM_005793.3         ------------------------------------------------------------
XM_005264792.1      ------------------------------------------------------------
XM_005264790.1      ACTAGGACCCCGAGCAGGGCTGCCCAGGGAGCGCCTGAAGGACCCTGCGAGGGCCGCAGG
XM_005264789.1      ACTAGGACCCCGAGCAGGGCTGCCCAGGGAGCGCCTGAAGGACCCTGCGAGGGCCGCAGG
                                                                                

HsCD00037958        ------------------------------ATGACCCAGAATCTGGGGAGTGAGATGGCC
NM_005793.3         ------------------------------ATGACCCAGAATCTGGGGAGTGAGATGGCC
XM_005264792.1      ------------------------------------------------------ATGGCC
XM_005264790.1      CTATCCAGCGTGGCGCCTGGTGGCCGGGCCCTGTGCCGGAGGACAAGGAGTGAGATGGCC
XM_005264789.1      CTATCCAGCGTGGCGCCTGGTGGCCGGGCCCTGTGCCGGAGGACAAGGAGTGAGATGGCC
                                                                          ******

HsCD00037958        TCAATCTTGCGAAGCCCTCAGGCTCTCCAGCTCACTCTAGCCCTGATCAAGCCTGACGCA
NM_005793.3         TCAATCTTGCGAAGCCCTCAGGCTCTCCAGCTCACTCTAGCCCTGATCAAGCCTGACGCA
XM_005264792.1      TCAATCTTGCGAAGCCCTCAGGCTCTCCAGCTCACTCTAGCCCTGATCAAGCCTGACGCA
XM_005264790.1      TCAATCTTGCGAAGCCCTCAGGCTCTCCAGCTCACTCTAGCCCTGATCAAGCCTGACGCA
XM_005264789.1      TCAATCTTGCGAAGCCCTCAGGCTCTCCAGCTCACTCTAGCCCTGATCAAGCCTGACGCA
                    ************************************************************

HsCD00037958        GTCGCCCATCCACTGATTCTGGAGGCTGTTCATCAGCAGATTCTAAGCAACAAGTTCCTG
NM_005793.3         GTCGCCCATCCACTGATTCTGGAGGCTGTTCATCAGCAGATTCTAAGCAACAAGTTCCTG
XM_005264792.1      GTCGCCCATCCACTGATTCTGGAGGCTGTTCATCAGCAGATTCTAAGCAACAAGTTCCTG
XM_005264790.1      GTCGCCCATCCACTGATTCTGGAGGCTGTTCATCAGCAGATTCTAAGCAACAAGTTCCTG
XM_005264789.1      GTCGCCCATCCACTGATTCTGGAGGCTGTTCATCAGCAGATTCTAAGCAACAAGTTCCTG
                    ************************************************************

HsCD00037958        ATTGTACGAATGAGAGAACTACTGTGGAGAAAGGAAGATTGCCAGAGGTTTTACCGAGAG
NM_005793.3         ATTGTACGAATGAGAGAACTACTGTGGAGAAAGGAAGATTGCCAGAGGTTTTACCGAGAG
XM_005264792.1      ATTGTACGAATGAGAGAACTACTGTGGAGAAAGGAAGATTGCCAGAGGTTTTACCGAGAG
XM_005264790.1      ATTGTACGAATGAGAGAACTACTGTGGAGAAAGGAAGATTGCCAGAGGTTTTACCGAGAG
XM_005264789.1      ATTGTACGAATGAGAGAACTACTGTGGAGAAAGGAAGATTGCCAGAGGTTTTACCGAGAG
                    ************************************************************

HsCD00037958        CATGAAGGGCGTTTTTTCTATCAGAGGCTGGTGGAGTTCATGGCCAGCGGGCCAATCCGA
NM_005793.3         CATGAAGGGCGTTTTTTCTATCAGAGGCTGGTGGAGTTCATGGCCAGCGGGCCAATCCGA
XM_005264792.1      CATGAAGGGCGTTTTTTCTATCAGAGGCTGGTGGAGTTCATGGCCAGCGGGCCAATCCGA
XM_005264790.1      CATGAAG----------------------------------------CGGGCCAATCCGA
XM_005264789.1      CATGAAGGGCGTTTTTTCTATCAGAGGCTGGTGGAGTTCATGGCCAGCGGGCCAATCCGA
                    *******                                        *************

HsCD00037958        GCCTACATCCTTGCCCACAAGGATGCCATCCAGCTCTGGAGGACGCTCATGGGACCCACC
NM_005793.3         GCCTACATCCTTGCCCACAAGGATGCCATCCAGCTCTGGAGGACGCTCATGGGACCCACC
XM_005264792.1      GCCTACATCCTTGCCCACAAGGATGCCATCCAGCTCTGGAGGACGCTCATGGGACCCACC
XM_005264790.1      GCCTACATCCTTGCCCACAAGGATGCCATCCAGCTCTGGAGGACGCTCATGGGACCCACC
XM_005264789.1      GCCTACATCCTTGCCCACAAGGATGCCATCCAGCTCTGGAGGACGCTCATGGGACCCACC
                    ************************************************************

HsCD00037958        AGAGTGTTCCGAGCACGCCATGTGGCCCCAGATTCTATCCGTGGGAGTTTCGGCCTCACT
NM_005793.3         AGAGTGTTCCGAGCACGCCATGTGGCCCCAGATTCTATCCGTGGGAGTTTCGGCCTCACT
XM_005264792.1      AGAGTGTTCCGAGCACGCCATGTGGCCCCAGATTCTATCCGTGGGAGTTTCGGCCTCACT
XM_005264790.1      AGAGTGTTCCGAGCACGCCATGTGGCCCCAGATTCTATCCGTGGGAGTTTCGGCCTCACT
XM_005264789.1      AGAGTGTTCCGAGCACGCCATGTGGCCCCAGATTCTATCCGTGGGAGTTTCGGCCTCACT
                    ************************************************************

HsCD00037958        GACACCCGCAACACCACCCATGGTTCGGACTCTGTGGTTTCAGCCAGCAGAGAGATTGCA
NM_005793.3         GACACCCGCAACACCACCCATGGTTCGGACTCTGTGGTTTCAGCCAGCAGAGAGATTGCA
XM_005264792.1      GACACCCGCAACACCACCCATGGTTCGGACTCTGTGGTTTCAGCCAGCAGAGAGATTGCA
XM_005264790.1      GACACCCGCAACACCACCCATGGTTCGGACTCTGTGGTTTCAGCCAGCAGAGAGATTGCA
XM_005264789.1      GACACCCGCAACACCACCCATGGTTCGGACTCTGTGGTTTCAGCCAGCAGAGAGATTGCA
                    ************************************************************

HsCD00037958        GCCTTCTTCCCTGACTTCAGTGAACAGCGCTGGTATGAGGAGGAAGAGCCCCAGTTGCGC
NM_005793.3         GCCTTCTTCCCTGACTTCAGTGAACAGCGCTGGTATGAGGAGGAAGAGCCCCAGTTGCGC
XM_005264792.1      GCCTTCTTCCCTGACTTCAGTGAACAGCGCTGGTATGAGGAGGAAGAGCCCCAGTTGCGC
XM_005264790.1      GCCTTCTTCCCTGACTTCAGTGAACAGCGCTGGTATGAGGAGGAAGAGCCCCAGTTGCGC
XM_005264789.1      GCCTTCTTCCCTGACTTCAGTGAACAGCGCTGGTATGAGGAGGAAGAGCCCCAGTTGCGC
                    ************************************************************

HsCD00037958        TGTGGCCCTGTGTGCTATAGCCCAGAGGGAGGTGTCCACTATGTAGCTGGAACAGGAGGC
NM_005793.3         TGTGGCCCTGTGTGCTATAGCCCAGAGGGAGGTGTCCACTATGTAGCTGGAACAGGAGGC
XM_005264792.1      TGTGGCCCTGTGTGCTATAGCCCAGAGGGAGGTGTCCACTATGTAGCTGGAACAGGAGGC
XM_005264790.1      TGTGGCCCTGTGTGCTATAGCCCAGAGGGAGGTGTCCACTATGTAG--------------
XM_005264789.1      TGTGGCCCTGTGTGCTATAGCCCAGAGGGAGGTGTCCACTATGTAGCTGGAACAGGAGGC
                    **********************************************              

HsCD00037958        CTAGGACCAGCCTTG
NM_005793.3         CTAGGACCAGCCTGA
XM_005264792.1      CTAGGACCAGCCTGA
XM_005264790.1      ---------------
XM_005264789.1      CTAGGACCAGCCTGA
                                   

Pathways

Panther : De novo purine biosynthesis

Protein

SMART domain : NDK :
UniProt : O75414
HPRD : 09219

Clone

Cloning Information : 138521
HIP Master Clone ID : 87185
Original Clone ID : FLH138521.01L

Organism


TAX_ID : 9606
Species Specific ID: 10201

Genome

Chromosome : 3
Map Location : 3p21
Ensembl : ENSG00000172113

Reagents

Labome : NME6-antibody